##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512940_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2459100 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.54689317229881 31.0 31.0 34.0 27.0 34.0 2 30.675219389207435 31.0 31.0 34.0 27.0 34.0 3 30.764925379203774 31.0 31.0 34.0 27.0 34.0 4 34.253560652271155 37.0 35.0 37.0 30.0 37.0 5 33.9280797852873 37.0 35.0 37.0 30.0 37.0 6 33.80667276645927 37.0 35.0 37.0 29.0 37.0 7 33.68750152494815 37.0 35.0 37.0 28.0 37.0 8 33.573431743320725 37.0 35.0 37.0 28.0 37.0 9 35.040978813387014 39.0 35.0 39.0 28.0 39.0 10 34.92701435484527 39.0 35.0 39.0 28.0 39.0 11 34.97146273026717 39.0 35.0 39.0 27.0 39.0 12 34.8227863852629 39.0 35.0 39.0 27.0 39.0 13 34.809229799520146 39.0 35.0 39.0 27.0 39.0 14 35.88266438941076 40.0 36.0 41.0 27.0 41.0 15 35.897745516652435 40.0 36.0 41.0 27.0 41.0 16 35.878701557480376 40.0 36.0 41.0 27.0 41.0 17 35.852095482086945 40.0 36.0 41.0 27.0 41.0 18 35.77335366597536 40.0 36.0 41.0 27.0 41.0 19 35.76036314098654 40.0 36.0 41.0 27.0 41.0 20 35.73528364035623 40.0 36.0 41.0 27.0 41.0 21 35.683665568704 40.0 36.0 41.0 27.0 41.0 22 35.64220080517262 40.0 36.0 41.0 27.0 41.0 23 35.54020820625432 40.0 36.0 41.0 27.0 41.0 24 35.53076654060428 40.0 36.0 41.0 27.0 41.0 25 35.43155910699036 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6904644542964355 1101 2 0.6125265764020646 1101 3 0.7019109315097012 1101 4 0.4134819796199949 1101 5 0.6996625462675325 1101 6 0.5490430436390596 1101 7 0.6477176417889154 1101 8 0.6168695190087092 1101 9 0.540526986511189 1101 10 0.8820447785939152 1101 11 0.9000268047359512 1101 12 1.0001797558842966 1101 13 1.007971963824815 1101 14 1.1840714674311101 1101 15 1.171503414353296 1101 16 1.2881466321169768 1101 17 1.180848926509448 1101 18 0.6523776269613109 1101 19 0.924645101008025 1101 20 0.956933171274919 1101 21 1.1115590666200745 1101 22 1.0411175971883608 1101 23 1.0661044825207142 1101 24 1.0242367831583294 1101 25 1.0372875338067686 1102 1 0.6169750028546268 1102 2 0.5850882310513974 1102 3 0.5765726625831498 1102 4 0.5286553119774169 1102 5 0.6934301065884014 1102 6 0.6314750174541501 1102 7 0.7413749260823437 1102 8 0.7143155582351852 1102 9 0.7898095633277222 1102 10 0.8358637257709276 1102 11 0.8358918739965588 1102 12 0.8393664580306606 1102 13 0.8999518488140978 1102 14 0.9724512132387204 1102 15 1.0386752674051252 1102 16 0.991381676734882 1102 17 0.9631367580916219 1102 18 1.0587148388734136 1102 19 1.0299502013529178 1102 20 1.0164499895842312 1102 21 1.031726885176127 1102 22 1.051562369541415 1102 23 1.084267361671401 1102 24 1.0422668564631579 1102 25 1.0438711441644273 1103 1 0.4697915391558425 1103 2 0.36018047650642515 1103 3 0.49122508769075424 1103 4 0.3860391857298566 1103 5 0.4355583974933026 1103 6 0.4897752528348036 1103 7 0.510245605750562 1103 8 0.5827397374978105 1103 9 0.7100447667357699 1103 10 0.7612523641884081 1103 11 0.7724455843506774 1103 12 0.7509848849101957 1103 13 0.832231997492471 1103 14 0.8654595713430098 1103 15 0.7772551235955447 1103 16 0.8755821621192084 1103 17 0.9993093977143914 1103 18 1.1240432182918525 1103 19 1.0344889436131766 1103 20 1.0444842855346224 1103 21 0.6922890901713785 1103 22 0.9175730592961955 1103 23 0.9942022726468807 1103 24 1.0906643902698718 1103 25 1.0008654309422056 1104 1 0.4475941668429755 1104 2 0.5218906105657943 1104 3 0.47608086823928275 1104 4 0.3877107630623371 1104 5 0.473269830767471 1104 6 0.4704098798798242 1104 7 0.5008979774205713 1104 8 0.42733850817679553 1104 9 0.48384893620140446 1104 10 0.5729488676667103 1104 11 0.5211959213677275 1104 12 0.5257456138740793 1104 13 0.5745676828987953 1104 14 0.7663054577453892 1104 15 0.6753135033640447 1104 16 0.6960691469072628 1104 17 0.577843791032933 1104 18 0.6388828363303318 1104 19 0.6472675401191523 1104 20 0.7381509268320343 1104 21 0.549171668285581 1104 22 0.7667057952641727 1104 23 0.5884740763130978 1104 24 0.6874587201661839 1104 25 0.8102132909341648 1105 1 0.48475318104306453 1105 2 0.5498855217773624 1105 3 0.5714435948906988 1105 4 0.3785140570151029 1105 5 0.4082109354770438 1105 6 0.5492355353283997 1105 7 0.6375131305766573 1105 8 0.6806185216138658 1105 9 0.8350010950830864 1105 10 0.7802608051241506 1105 11 0.7100398189393573 1105 12 0.7326137159293822 1105 13 0.7480188020682661 1105 14 0.9880138485322902 1105 15 0.8659246087249954 1105 16 0.9258895311665043 1105 17 0.7116013301513462 1105 18 0.9039604122922427 1105 19 0.9193686011962043 1105 20 0.9699509471259162 1105 21 0.8502923662323383 1105 22 0.8930547129273521 1105 23 0.9836070835751727 1105 24 0.9585686075893776 1105 25 0.9650513848902094 1106 1 0.33804840924086577 1106 2 0.367192300967659 1106 3 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1107 16 0.6525064575917696 1107 17 0.5283785543497075 1107 18 0.7626128783198425 1107 19 0.8752284780021213 1107 20 0.8243138005815709 1107 21 0.7736639565014372 1107 22 0.7187524834624028 1107 23 0.8216146301218785 1107 24 0.8481500550231971 1107 25 0.8399150677321217 1108 1 0.3577850642475475 1108 2 0.30099987887821555 1108 3 0.21980661659505074 1108 4 0.2640631274626344 1108 5 0.28523449499206777 1108 6 0.3508103812042904 1108 7 0.3719874900395226 1108 8 0.44126756351379726 1108 9 0.4083636662633978 1108 10 0.5206681839300202 1108 11 0.4946674463507179 1108 12 0.5155941875239947 1108 13 0.575453728275086 1108 14 0.5704440889565277 1108 15 0.5636709178690822 1108 16 0.46095942024714986 1108 17 0.48822299561673077 1108 18 0.5672003697787318 1108 19 0.4681252445972106 1108 20 0.5840472760165838 1108 21 0.6486246359299344 1108 22 0.6712594301235981 1108 23 0.6051453382536565 1108 24 0.6328955130251472 1108 25 0.6101543414384807 1109 1 -0.10423602137949217 1109 2 0.010984868136638681 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-1.4486360291999674 2216 12 -0.9606346888503907 2216 13 -1.332642832511013 2216 14 -1.3524121470724282 2216 15 -1.3622723920204294 2216 16 -1.295560760745417 2216 17 -1.4512213675466867 2216 18 -1.1818424138485426 2216 19 -1.4900200049150385 2216 20 -1.8829307086313634 2216 21 -1.7342721755003154 2216 22 -1.8746703807381024 2216 23 -1.020705752359632 2216 24 -1.3591827032883685 2216 25 -1.2073087446888167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59850.0 3 1.0 4 946.0 5 8186.0 6 9490.0 7 7509.0 8 5988.0 9 5436.0 10 4496.0 11 4138.0 12 3867.0 13 3668.0 14 3686.0 15 3752.0 16 3804.0 17 3976.0 18 4382.0 19 5166.0 20 6624.0 21 8697.0 22 11123.0 23 13851.0 24 16329.0 25 18856.0 26 21690.0 27 24180.0 28 27349.0 29 33238.0 30 42048.0 31 53989.0 32 68853.0 33 89232.0 34 116750.0 35 151901.0 36 209248.0 37 326534.0 38 1080267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.5758145516405 19.510455074516315 13.605230386052302 27.30849998779088 2 13.753585157478538 20.724558648967488 39.913675629712856 25.608180563841117 3 16.91598552315888 24.892643650115893 30.219104550445287 27.97226627627994 4 11.618571881838964 17.38075286575494 37.748602715653654 33.25207253675244 5 12.819777154580036 38.09636015349059 35.9941869243238 13.089675767605573 6 31.74365968017692 37.57959865227125 17.913323899720453 12.763417767831381 7 27.548824855387625 31.785634811602186 22.594394177996787 18.0711461550134 8 24.95222095245537 36.974878151219464 20.53082766008016 17.542073236245006 9 26.18998114063552 14.966319657072871 20.148148750948565 38.69555045134304 10 14.648772839404636 28.992592779578498 34.694641989774915 21.663992391241948 11 34.06057934319307 22.343701008191687 24.933091550604587 18.66262809801066 12 23.03830912567308 24.76620366321939 31.846613822098814 20.348873389008716 13 28.659511146068112 21.942060129472846 25.9563039317677 23.44212479269135 14 20.85955340638961 21.914483891761748 27.670909190734637 29.555053511114004 15 22.7986053580572 30.84854274720712 23.35777169631684 22.995080198418847 16 22.240431090551223 27.9459623698754 27.475718006320093 22.337888533253285 17 21.72069894419625 28.60303097116534 27.694081331954557 21.98218875268385 18 21.628252352722537 27.54452300182264 29.37611271921941 21.45111192623541 19 23.36805633218584 26.964255576996198 27.547545023658138 22.120143067159827 20 23.57561102177628 27.266346037315657 27.854979881362258 21.3030630595458 21 22.92055529394159 26.654532116299745 27.289237418333745 23.135675171424925 22 23.191168104259834 27.73541562376606 27.226527568971736 21.846888703002374 23 22.331318231318964 27.52671914661392 27.730127062849874 22.41183555921724 24 23.031900314668224 26.795760071375764 28.096114916932958 22.076224697023054 25 22.730095418039024 27.159187448072746 27.799618457979747 22.311098675908482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 150.0 2 360.0 3 570.0 4 570.0 5 570.0 6 1302.0 7 2034.0 8 2034.0 9 2034.0 10 3127.5 11 4221.0 12 4221.0 13 4221.0 14 7326.0 15 10431.0 16 10431.0 17 10431.0 18 19515.5 19 28600.0 20 28600.0 21 28600.0 22 45490.0 23 62380.0 24 62380.0 25 62380.0 26 89460.0 27 116540.0 28 116540.0 29 116540.0 30 149679.0 31 182818.0 32 182818.0 33 182818.0 34 208517.5 35 234217.0 36 234217.0 37 234217.0 38 254747.0 39 275277.0 40 275277.0 41 275277.0 42 290317.5 43 305358.0 44 305358.0 45 305358.0 46 311711.5 47 318065.0 48 318065.0 49 318065.0 50 307712.0 51 297359.0 52 297359.0 53 297359.0 54 266787.5 55 236216.0 56 236216.0 57 236216.0 58 204353.5 59 172491.0 60 172491.0 61 172491.0 62 140934.5 63 109378.0 64 109378.0 65 109378.0 66 84403.0 67 59428.0 68 59428.0 69 59428.0 70 43425.5 71 27423.0 72 27423.0 73 27423.0 74 19000.5 75 10578.0 76 10578.0 77 10578.0 78 7255.5 79 3933.0 80 3933.0 81 3933.0 82 2579.5 83 1226.0 84 1226.0 85 1226.0 86 785.0 87 344.0 88 344.0 89 344.0 90 199.5 91 55.0 92 55.0 93 55.0 94 31.0 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07807734537025741 2 1.219958521410272E-4 3 0.0 4 0.030905615875726895 5 0.07649139929242406 6 0.0784433329266805 7 0.03972998251392786 8 0.07954129559594973 9 0.05835468260745802 10 0.12130454231222805 11 0.005815135618722296 12 0.01801472083282502 13 0.008377048513683868 14 0.010776300272457403 15 0.010613639136269367 16 0.024033182871782358 17 0.03570411939327396 18 0.0013826196575983084 19 0.004676507665406043 20 0.0075637428327436865 21 2.439917042820544E-4 22 5.286486926111178E-4 23 8.133056809401814E-5 24 8.133056809401814E-5 25 0.005327152210158188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2459100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.94265180947056 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20872986931448 56.79953917138815 2 12.586205380920157 17.606251611203668 3 3.30053388807871 6.925442775577418 4 1.1691514379588854 3.270942077508006 5 0.5907152157191101 2.0658094325799006 6 0.30503905348740157 1.2801144183815876 7 0.18883195135358427 0.924518519681864 8 0.1364798023472972 0.7636607435681897 9 0.08533252819090281 0.5371544976550332 >10 0.39463045809343633 4.702160461787893 >50 0.019159081037069488 0.9361603500752318 >100 0.012344262320595519 1.7043420363371677 >500 0.0018593117899315568 0.9178752569791537 >1k 9.877593883896758E-4 1.566028647276797 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4828 0.19633199137895976 No Hit TATCAACGCAGAGTACTTTTTTTTT 3627 0.1474929852385019 No Hit GTCCTACAGTGGACATTTCTAAATT 3363 0.1367573502500915 No Hit CTTTAGGACGTGAAATATGGCGAGG 3179 0.12927493798544182 No Hit CTGTAGGACGTGGAATATGGCAAGA 3163 0.12862429344068968 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3147 0.12797364889593754 No Hit GGTATCAACGCAGAGTACTTTTTTT 2931 0.11918994754178357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.066528404700907E-5 0.0 4 0.0 0.0 0.0 4.066528404700907E-5 0.0 5 0.0 0.0 0.0 4.066528404700907E-5 0.0 6 0.0 0.0 0.0 4.066528404700907E-5 0.0 7 0.0 0.0 0.0 4.066528404700907E-5 0.0 8 0.0 0.0 0.0 4.066528404700907E-5 0.0 9 0.0 0.0 0.0 4.066528404700907E-5 0.0 10 0.0 0.0 0.0 1.219958521410272E-4 0.0 11 0.0 0.0 0.0 1.219958521410272E-4 0.0 12 0.0 0.0 0.0 2.0332642023504534E-4 0.0 13 0.0 0.0 0.0 2.439917042820544E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 65 5.3878182E-5 13.171231 6 GGACGTC 60 4.0481723E-4 12.683407 6 TAGGACC 680 0.0 12.594512 4 GACGGGT 55 0.0030581784 12.09678 7 GGTTTCG 55 0.0030600447 12.095795 8 ACTGCGC 75 2.0669788E-4 11.404607 8 GGTATCA 1135 0.0 11.057651 1 AATACCG 70 0.0014713263 10.875261 5 GGCGAGG 950 0.0 10.788325 19 GTATCAA 2370 0.0 10.751555 1 TGGCGAG 1995 0.0 10.750053 18 GTAGGAC 1935 0.0 10.613691 3 TGTAGGA 2070 0.0 10.563909 2 GACGTGG 975 0.0 10.235737 7 AGGACGT 2005 0.0 10.109111 5 GGACGTG 1990 0.0 9.942772 6 AGGACCT 1400 0.0 9.923675 5 CTGTAGG 2175 0.0 9.879478 1 GGCGAGA 1175 0.0 9.853168 19 CAGCGCT 155 4.0547093E-8 9.808218 9 >>END_MODULE