FastQCFastQC Report
Thu 26 May 2016
SRR1512939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534735
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51910.20479458404922013No Hit
CTGTAGGACGTGGAATATGGCAAGA48040.18952671581052852No Hit
GTCCTAAAGTGTGTATTTCTCATTT46810.1846741375331149No Hit
CTTTAGGACGTGAAATATGGCGAGG40100.15820194221486664No Hit
GTACATGGGAAGCAGTGGTATCAAC37630.14845733380412549No Hit
GTATCAACGCAGAGTACTTTTTTTT35750.14104038489230628No Hit
CCCATGTACTCTGCGTTGATACCAC30240.11930241228372984No Hit
GTCCTACAGTGTGCATTTCTCATTT27400.10809808520417322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8900.013.1255044
TGTAGGA28600.011.1576862
GAACCGC600.0059018911.0771416
GGCGAGG11200.011.02105919
GTAGGAC27500.011.0168873
GTCCTAC23450.010.9710231
AGGACGT26250.010.8898255
GGACGTG25700.010.8616316
TTTAACG700.00149548610.85407453
CGTAGAC700.00149548610.85407453
CTGTGCG1408.429197E-910.851299
CTGTAGG28800.010.818861
GACGTGG13600.010.7513437
GTCCTAA12900.010.5626411
ACCGTGC909.57176E-510.5498678
AGGACCT18200.010.4361985
TTAGGAC15800.010.338853
GATATAC6600.010.2503971
TAGGACG29100.010.2479634
TCCTACA26250.010.130472
Produced by FastQC (version 0.11.5)