##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512939_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2534735 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.928180263420042 31.0 30.0 34.0 23.0 34.0 2 29.040512321800897 31.0 30.0 34.0 22.0 34.0 3 29.271590126778538 31.0 30.0 34.0 23.0 34.0 4 32.66807812256508 35.0 33.0 37.0 25.0 37.0 5 32.79914981250506 35.0 35.0 37.0 26.0 37.0 6 32.83315336711727 37.0 35.0 37.0 26.0 37.0 7 32.65210209351273 36.0 33.0 37.0 25.0 37.0 8 32.64644824804171 37.0 33.0 37.0 25.0 37.0 9 33.955640333210376 39.0 34.0 39.0 23.0 39.0 10 33.83657857724772 38.0 34.0 39.0 23.0 39.0 11 33.89414041310038 38.0 34.0 39.0 23.0 39.0 12 33.781942096511074 38.0 34.0 39.0 22.0 39.0 13 33.77669460515596 38.0 34.0 39.0 22.0 39.0 14 34.76914825415675 39.0 34.0 41.0 22.0 41.0 15 34.70539326596271 39.0 34.0 41.0 19.0 41.0 16 34.59177152641203 39.0 34.0 41.0 18.0 41.0 17 34.538520594855086 39.0 34.0 41.0 18.0 41.0 18 34.23198204151519 39.0 33.0 41.0 17.0 41.0 19 34.46762876592622 39.0 34.0 41.0 18.0 41.0 20 34.540707805746955 39.0 34.0 41.0 18.0 41.0 21 34.47571008409163 39.0 34.0 41.0 18.0 41.0 22 34.391447626674974 39.0 34.0 41.0 18.0 41.0 23 34.11833071307257 39.0 34.0 41.0 10.0 41.0 24 34.11690058329569 39.0 34.0 41.0 8.0 41.0 25 34.03701885995972 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.60257673787466 1101 2 0.7856648864679272 1101 3 0.9281838830053317 1101 4 0.8603519461720666 1101 5 0.7679308890729288 1101 6 0.8354759868849584 1101 7 0.8315042981044343 1101 8 0.5968231816632255 1101 9 0.5924437153242508 1101 10 0.7043416099089086 1101 11 0.7693729890115435 1101 12 0.9940178453981972 1101 13 0.834169673527974 1101 14 0.8197300907739447 1101 15 0.5331359206517945 1101 16 1.0499710360827308 1101 17 1.0717892796470068 1101 18 1.1991905142382606 1101 19 0.6619096162903233 1101 20 0.8841289935245484 1101 21 0.9353123320374195 1101 22 0.9030678188854182 1101 23 1.1842610211410758 1101 24 0.8535075867124391 1101 25 0.9829603805749301 1102 1 0.5855362696854556 1102 2 0.6428335417473718 1102 3 0.6686812356544607 1102 4 0.9390574692219644 1102 5 0.7151694449810293 1102 6 0.6646138404984754 1102 7 0.8592214983920812 1102 8 0.7867536260365142 1102 9 0.8156762811360707 1102 10 0.8889269499137171 1102 11 0.8438955656154548 1102 12 0.8773024067767352 1102 13 0.8602472385904889 1102 14 0.8930297971988495 1102 15 0.9530572629034282 1102 16 1.0378189489826752 1102 17 0.9523911813624224 1102 18 1.2161888679320967 1102 19 1.1797813235432386 1102 20 1.1558394538757355 1102 21 0.9525510415792056 1102 22 1.285776456116949 1102 23 1.4199400712213404 1102 24 1.2857198254408502 1102 25 1.1424588905977728 1103 1 0.40456115150539773 1103 2 0.6337631832418431 1103 3 0.6707279206804095 1103 4 0.745923231038411 1103 5 0.2741228723588378 1103 6 0.6664236190006321 1103 7 0.5148121020626846 1103 8 0.6188247588790219 1103 9 0.8249847170378288 1103 10 0.7569376220257169 1103 11 0.7273078938094386 1103 12 0.9998697241362464 1103 13 0.8995994807331584 1103 14 1.0131535037750936 1103 15 1.1106242992535584 1103 16 1.0882995530904083 1103 17 0.9844001018492534 1103 18 1.298402847212337 1103 19 1.3276910040043433 1103 20 1.1979463685800198 1103 21 1.0999603692174134 1103 22 1.1909490583263036 1103 23 1.5444092757149903 1103 24 1.3435531181839337 1103 25 1.2341620104264166 1104 1 0.5545547840429776 1104 2 0.49768859340615634 1104 3 0.5944261581974963 1104 4 0.912307197716892 1104 5 0.6345589642610108 1104 6 0.703092403040344 1104 7 0.8767244735051563 1104 8 0.8795724728577241 1104 9 1.0058468612207037 1104 10 1.0281315544733332 1104 11 0.9077450290548512 1104 12 0.8253250488435881 1104 13 0.9250537314106921 1104 14 1.0582713222875881 1104 15 1.1706376325664323 1104 16 0.9850905310354676 1104 17 1.0539182153619322 1104 18 1.3158343509614667 1104 19 1.1637518616017957 1104 20 1.1682615912136143 1104 21 1.1858761049303865 1104 22 1.1550259578375233 1104 23 1.3623378251149916 1104 24 1.2680012586547633 1104 25 0.9810025727475136 1105 1 0.6074039224733419 1105 2 0.81415202078324 1105 3 0.8083576774275265 1105 4 0.8111791603185239 1105 5 0.592573843936421 1105 6 0.45601792390767315 1105 7 0.7181309397860716 1105 8 0.8371038508041053 1105 9 0.6964860587626376 1105 10 0.8998934925670525 1105 11 0.7984579644805336 1105 12 0.8292210562390494 1105 13 0.8390744043346672 1105 14 1.0048588940227035 1105 15 1.2279442621904337 1105 16 1.1443497181488311 1105 17 1.0158522583987448 1105 18 1.0343602331392674 1105 19 1.0865598043432243 1105 20 1.2043452297885935 1105 21 1.1302113558443097 1105 22 0.8215834668077164 1105 23 1.1477025967249261 1105 24 1.2359887484990324 1105 25 1.3504487852441969 1106 1 0.6300627385169584 1106 2 0.721666906435793 1106 3 0.7855619393242392 1106 4 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1.189449094578542 1107 18 1.4563651127219543 1107 19 1.307426699050012 1107 20 1.1302456651619934 1107 21 1.0432628709732725 1107 22 1.0641220354525274 1107 23 1.0936840423028755 1107 24 1.097723857264178 1107 25 1.1068901787266796 1108 1 0.42101133805196866 1108 2 0.5989567989866913 1108 3 0.48458492591903024 1108 4 0.5528155556111756 1108 5 0.5566433822202654 1108 6 0.6524986375777004 1108 7 0.6941577286737086 1108 8 0.6875506584309434 1108 9 0.800884832091775 1108 10 0.7737858797105233 1108 11 0.68688471453126 1108 12 0.8328869531957181 1108 13 0.746870309514513 1108 14 1.0716364235073854 1108 15 1.0913667163174807 1108 16 0.8958267141561791 1108 17 0.9432325883585051 1108 18 1.104488267447067 1108 19 1.0741276979290575 1108 20 1.0153508496521866 1108 21 0.9650857084413502 1108 22 0.8309988941798352 1108 23 1.2148484203863816 1108 24 1.1859927824376513 1108 25 1.312849699654123 1109 1 0.34806080694226793 1109 2 0.2358269009159315 1109 3 0.49577889962487376 1109 4 0.4667543826470819 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17 0.5486177924836682 1110 18 0.8520551940283099 1110 19 0.709269302217038 1110 20 0.7058409742795604 1110 21 0.6686255109582717 1110 22 0.6993420057936106 1110 23 0.6733361465522805 1110 24 0.8470488610993883 1110 25 0.9559765475541298 1111 1 0.38502151307633525 1111 2 0.4882728220067456 1111 3 0.5250327750171095 1111 4 0.4226029104912712 1111 5 0.4161530008255241 1111 6 0.5315445396053917 1111 7 0.5698002917335998 1111 8 0.37475035039591376 1111 9 0.7352766561231121 1111 10 0.6796807502954039 1111 11 0.45528880684560136 1111 12 0.5847680853075232 1111 13 0.6912515590023673 1111 14 0.5568853696089349 1111 15 0.7130800138971694 1111 16 0.6522505196047135 1111 17 0.7013532801383207 1111 18 0.8829795881019393 1111 19 0.6880948056989027 1111 20 0.6297650563203803 1111 21 0.7447961665905893 1111 22 0.5803628292845389 1111 23 0.8523865266934578 1111 24 0.987703791656422 1111 25 0.45897387961092306 1112 1 -0.12517367408311486 1112 2 0.16143635052638672 1112 3 0.1919337123919007 1112 4 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-3.8820063054994307 2216 24 -3.980004725957702 2216 25 -4.0938859215014105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124479.0 3 4.0 4 1070.0 5 6119.0 6 7523.0 7 6918.0 8 6007.0 9 5933.0 10 5326.0 11 5164.0 12 4862.0 13 4929.0 14 4862.0 15 5068.0 16 5224.0 17 5460.0 18 6156.0 19 7415.0 20 9253.0 21 11943.0 22 14954.0 23 18046.0 24 20811.0 25 23681.0 26 26802.0 27 29417.0 28 33523.0 29 39808.0 30 48798.0 31 60350.0 32 76726.0 33 96691.0 34 125436.0 35 163654.0 36 223438.0 37 343483.0 38 955402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47652057342465 18.53071117468595 12.695150500414323 25.29761775147507 2 14.557196584138115 19.887800848479444 41.650363163251974 23.904639404130467 3 18.81084686362883 24.259971110812923 29.590854914713756 27.338327110844496 4 12.29374917120792 16.569132309946134 38.28707621098363 32.85004230786232 5 13.178794198487262 37.75648423402445 36.03105201753652 13.033669549951771 6 32.242709642898575 36.859126885478 18.021985306825957 12.876178164797466 7 28.17686708688436 31.31970032854005 22.133482122070426 18.36995046250516 8 25.012272737317648 37.10222124706796 20.65083391474275 17.23467210087164 9 26.365709491788003 15.14944405619379 20.119140117096045 38.36570633492216 10 14.868288340333645 28.944128496408293 34.72526726678379 21.462315896474273 11 34.28209749077523 22.234156693469245 24.88496410517049 18.598781710585037 12 23.42521230842324 24.51555086937838 31.846519980727393 20.212716841470986 13 29.027947951502952 21.854568054692354 25.771779770536764 23.345704223267926 14 20.6834471333216 22.25256099303151 27.556356053604254 29.507635820042637 15 22.925272271571874 31.261160894868468 23.140824299051406 22.672742534508252 16 21.997368736741084 28.048642855338922 28.105585160117187 21.848403247802807 17 21.68836063083167 28.744157230433505 27.913374097071365 21.654108041663463 18 21.656708265650565 27.502524651140014 29.886221862037342 20.954545221172076 19 23.488148075362037 26.947812227093195 27.45206212478705 22.11197757275772 20 23.592582800273075 27.49063773869327 27.963624309915513 20.953155151118143 21 22.820789466830096 26.852219443144353 27.15888704640148 23.16810404362407 22 23.11199748399182 28.268235830781872 27.007232734308197 21.612533950918113 23 22.10772639649896 27.911984704659044 27.676832649002307 22.303456249839687 24 23.071911647312156 26.9795245613322 28.280602176538856 21.667961614816786 25 22.681326903201953 27.35499747248641 27.809634226756447 22.1540413975552 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 210.0 1 210.0 2 427.0 3 644.0 4 644.0 5 644.0 6 1226.0 7 1808.0 8 1808.0 9 1808.0 10 2697.5 11 3587.0 12 3587.0 13 3587.0 14 6562.5 15 9538.0 16 9538.0 17 9538.0 18 17752.0 19 25966.0 20 25966.0 21 25966.0 22 42933.0 23 59900.0 24 59900.0 25 59900.0 26 88606.0 27 117312.0 28 117312.0 29 117312.0 30 154640.5 31 191969.0 32 191969.0 33 191969.0 34 218910.0 35 245851.0 36 245851.0 37 245851.0 38 265642.0 39 285433.0 40 285433.0 41 285433.0 42 300114.0 43 314795.0 44 314795.0 45 314795.0 46 326584.5 47 338374.0 48 338374.0 49 338374.0 50 324546.5 51 310719.0 52 310719.0 53 310719.0 54 275226.5 55 239734.0 56 239734.0 57 239734.0 58 206543.5 59 173353.0 60 173353.0 61 173353.0 62 141587.0 63 109821.0 64 109821.0 65 109821.0 66 85153.0 67 60485.0 68 60485.0 69 60485.0 70 44310.0 71 28135.0 72 28135.0 73 28135.0 74 19581.0 75 11027.0 76 11027.0 77 11027.0 78 7569.0 79 4111.0 80 4111.0 81 4111.0 82 2731.5 83 1352.0 84 1352.0 85 1352.0 86 915.0 87 478.0 88 478.0 89 478.0 90 292.5 91 107.0 92 107.0 93 107.0 94 58.5 95 10.0 96 10.0 97 10.0 98 13.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3522261695995834 2 0.04047760416769406 3 0.036098448161247625 4 0.03657187043221481 5 0.041463900565542354 6 0.01960757238922412 7 0.01944976496556839 8 0.026156580470936806 9 0.022724269006424734 10 0.02221139487954362 11 0.020751676210728143 12 0.02240865415911328 13 0.022093039311801826 14 0.029865054926846397 15 0.0306146401892111 16 0.023316046845133713 17 0.024460150666637738 18 0.1065989146794438 19 0.026590550885990053 20 0.024657409946207395 21 0.028444788113944852 22 0.022171943023629688 23 0.024854669225777053 24 0.020830579922556006 25 0.02461795809029346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2534735.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.346072288666 #Duplication Level Percentage of deduplicated Percentage of total 1 80.50312211446702 54.21569081384202 2 12.637541988624024 17.021776326338507 3 3.569849643506604 7.212460564537928 4 1.3804998228316885 3.71884963451654 5 0.6189526302041829 2.0842014288495427 6 0.36037850657821147 1.4562046173178633 7 0.2107898762219037 0.9937109169231498 8 0.14635843217781025 0.7885332442802102 9 0.10499982939763759 0.6364193490819808 >10 0.42425541209108725 4.911587484891911 >50 0.022079648655403773 1.0294655909517074 >100 0.017063666058453636 2.290695314175901 >500 0.002406365666053329 1.1982900034639385 >1k 0.0016433716743611394 2.236931856628482 >5k 5.869184551289784E-5 0.2051828542003154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5191 0.20479458404922013 No Hit CTGTAGGACGTGGAATATGGCAAGA 4804 0.18952671581052852 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4681 0.1846741375331149 No Hit CTTTAGGACGTGAAATATGGCGAGG 4010 0.15820194221486664 No Hit GTACATGGGAAGCAGTGGTATCAAC 3763 0.14845733380412549 No Hit GTATCAACGCAGAGTACTTTTTTTT 3575 0.14104038489230628 No Hit CCCATGTACTCTGCGTTGATACCAC 3024 0.11930241228372984 No Hit GTCCTACAGTGTGCATTTCTCATTT 2740 0.10809808520417322 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.945185591393183E-5 2 0.0 0.0 0.0 0.0 3.945185591393183E-5 3 0.0 0.0 0.0 0.0 3.945185591393183E-5 4 0.0 0.0 0.0 7.890371182786366E-5 3.945185591393183E-5 5 0.0 0.0 0.0 7.890371182786366E-5 3.945185591393183E-5 6 0.0 0.0 0.0 7.890371182786366E-5 3.945185591393183E-5 7 0.0 0.0 0.0 7.890371182786366E-5 3.945185591393183E-5 8 0.0 0.0 0.0 1.5780742365572732E-4 3.945185591393183E-5 9 0.0 0.0 0.0 1.9725927956965915E-4 7.890371182786366E-5 10 0.0 0.0 0.0 2.36711135483591E-4 7.890371182786366E-5 11 0.0 0.0 0.0 2.761629913975228E-4 7.890371182786366E-5 12 0.0 0.0 0.0 2.761629913975228E-4 1.9725927956965915E-4 13 0.0 0.0 0.0 3.1561484731145465E-4 1.9725927956965915E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 890 0.0 13.125504 4 TGTAGGA 2860 0.0 11.157686 2 GAACCGC 60 0.00590189 11.077141 6 GGCGAGG 1120 0.0 11.021059 19 GTAGGAC 2750 0.0 11.016887 3 GTCCTAC 2345 0.0 10.971023 1 AGGACGT 2625 0.0 10.889825 5 GGACGTG 2570 0.0 10.861631 6 TTTAACG 70 0.001495486 10.8540745 3 CGTAGAC 70 0.001495486 10.8540745 3 CTGTGCG 140 8.429197E-9 10.85129 9 CTGTAGG 2880 0.0 10.81886 1 GACGTGG 1360 0.0 10.751343 7 GTCCTAA 1290 0.0 10.562641 1 ACCGTGC 90 9.57176E-5 10.549867 8 AGGACCT 1820 0.0 10.436198 5 TTAGGAC 1580 0.0 10.33885 3 GATATAC 660 0.0 10.250397 1 TAGGACG 2910 0.0 10.247963 4 TCCTACA 2625 0.0 10.13047 2 >>END_MODULE