FastQCFastQC Report
Thu 26 May 2016
SRR1512939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534735
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50470.19911351679761397No Hit
CTGTAGGACGTGGAATATGGCAAGA48950.1931168346986963No Hit
GTATCAACGCAGAGTACTTTTTTTT42230.16660518752453413No Hit
GTCCTAAAGTGTGTATTTCTCATTT41910.1653427281352883No Hit
CTTTAGGACGTGAAATATGGCGAGG41670.16439588359335394No Hit
GTACATGGGAAGCAGTGGTATCAAC36100.1424211998492939No Hit
CCCATGTACTCTGCGTTGATACCAC35590.1404091551976834No Hit
TATCAACGCAGAGTACTTTTTTTTT30080.11867118258910696No Hit
GTCCTACAGTGTGCATTTCTCATTT26880.10604658869664876No Hit
GGTATCAACGCAGAGTACTTTTTTT25710.10143072155471874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9050.012.8256124
AACCGTG657.989395E-411.6987657
GGCGAGG10700.011.17564919
TGCACCG1201.2505006E-711.0997615
CCGGTTT2150.011.03778212
GATATAC6550.010.8847291
AGGACCG1408.167262E-910.8732375
GCGTATT700.001501589110.84877611
GTCCTAC25150.010.7344061
TGTAGGA25150.010.470242
AGGACCT18000.010.3069225
GCACCGC1201.5001315E-610.304686
TTAGACT1303.766654E-710.2459344
GGACCTG18000.010.1989916
GAACCGT750.002620936610.1461466
TGCGTAT750.002647711410.13372210
GCCGGTT2350.010.09859511
AGGACGT24200.010.0644835
GTAGGAC25600.010.0639843
TTAGGAC15600.010.0554143
Produced by FastQC (version 0.11.5)