##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512939_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2534735
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.51494771642795	31.0	31.0	34.0	27.0	34.0
2	30.68560618762908	31.0	31.0	34.0	27.0	34.0
3	30.78649957490625	31.0	31.0	34.0	27.0	34.0
4	34.25250608051729	37.0	35.0	37.0	30.0	37.0
5	33.91124910493602	37.0	35.0	37.0	30.0	37.0
6	33.77412234415038	37.0	35.0	37.0	29.0	37.0
7	33.64908284297964	37.0	35.0	37.0	28.0	37.0
8	33.54211150278037	37.0	35.0	37.0	28.0	37.0
9	35.01340258449108	39.0	35.0	39.0	28.0	39.0
10	34.9062063687131	39.0	35.0	39.0	27.0	39.0
11	34.92522926459768	39.0	35.0	39.0	27.0	39.0
12	34.78326057753572	39.0	35.0	39.0	27.0	39.0
13	34.75994334713491	39.0	35.0	39.0	27.0	39.0
14	35.824217127234206	40.0	36.0	41.0	27.0	41.0
15	35.83782959559875	40.0	36.0	41.0	27.0	41.0
16	35.81485875249287	40.0	36.0	41.0	27.0	41.0
17	35.78988848932926	40.0	36.0	41.0	27.0	41.0
18	35.715998713869496	40.0	36.0	41.0	27.0	41.0
19	35.70186193033986	40.0	36.0	41.0	27.0	41.0
20	35.67772567941027	40.0	36.0	41.0	27.0	41.0
21	35.62554349863004	40.0	36.0	41.0	27.0	41.0
22	35.58252953464563	40.0	36.0	41.0	27.0	41.0
23	35.47084724833168	40.0	36.0	41.0	26.0	41.0
24	35.459915533576485	40.0	36.0	41.0	26.0	41.0
25	35.36709715216778	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6615942452496242
1101	2	0.6623813760784039
1101	3	0.6201149179220522
1101	4	0.5466313998753023
1101	5	0.6977295613041079
1101	6	0.7204669740047933
1101	7	0.7261516574387414
1101	8	0.8168180643155409
1101	9	0.6304641446196726
1101	10	0.9672556513794817
1101	11	0.958062974564335
1101	12	0.9984047026468019
1101	13	1.057187551091829
1101	14	1.083105701721884
1101	15	1.0420970142263428
1101	16	1.2300030987824755
1101	17	1.1399497536525445
1101	18	0.6580432519188903
1101	19	1.0531240491210667
1101	20	0.9808214806979478
1101	21	1.1536875446065338
1101	22	0.9803932976859713
1101	23	1.059954394396442
1101	24	1.0027252088454262
1101	25	1.060187724940512
1102	1	0.5018247874094008
1102	2	0.5076046085094497
1102	3	0.4495725047843777
1102	4	0.30480841767847977
1102	5	0.455816218246035
1102	6	0.4416884210507632
1102	7	0.588591902023559
1102	8	0.5535548283697835
1102	9	0.7727647119322469
1102	10	0.8347604167188578
1102	11	0.6811819323134571
1102	12	0.7482387378434652
1102	13	0.7440372359002083
1102	14	0.8067857593156447
1102	15	0.9015950390902034
1102	16	0.8688322861627356
1102	17	0.877634868759209
1102	18	0.7702490703767069
1102	19	0.8177919550994162
1102	20	0.8452055282392124
1102	21	0.8053507369957913
1102	22	0.8842663892591887
1102	23	0.7474466453292905
1102	24	0.7936613069381053
1102	25	0.8334833075140367
1103	1	0.542092651800516
1103	2	0.48689577794561956
1103	3	0.49968974814474976
1103	4	0.5373932512897
1103	5	0.5669452827345651
1103	6	0.6614393218419607
1103	7	0.6715447018137368
1103	8	0.7309619079329082
1103	9	0.8028666715043684
1103	10	0.821080333415189
1103	11	0.8289038828932718
1103	12	0.8558875791481029
1103	13	0.8563590291147705
1103	14	1.0595158435560137
1103	15	0.7952005924601266
1103	16	0.8844525051002705
1103	17	0.9557839692169381
1103	18	1.0390056662914589
1103	19	1.045718277560809
1103	20	1.0798702215955274
1103	21	0.740475427104883
1103	22	0.8415908208396488
1103	23	0.8607831946589286
1103	24	1.001837743364412
1103	25	1.0736471984324325
1104	1	0.38104507892887085
1104	2	0.4173005644361645
1104	3	0.40576916600013746
1104	4	0.4212313019153697
1104	5	0.47545456516502327
1104	6	0.5659295591667473
1104	7	0.5220021617946244
1104	8	0.47704579068363273
1104	9	0.6646955499154998
1104	10	0.7019119956092865
1104	11	0.7342440102322954
1104	12	0.6735756225880039
1104	13	0.6869478327120504
1104	14	0.7817836268045255
1104	15	0.73396900248175
1104	16	0.8004353727905382
1104	17	0.7238680739338221
1104	18	0.8700251046966159
1104	19	0.9245228363570348
1104	20	0.9071014034425815
1104	21	0.6442408221714047
1104	22	0.8103002410527864
1104	23	0.7253897187251255
1104	24	0.8284638884208348
1104	25	0.9781325770509568
1105	1	0.4854005032687212
1105	2	0.5472623590273962
1105	3	0.5182204707452911
1105	4	0.42585759300560255
1105	5	0.3529627303843199
1105	6	0.40618054919717395
1105	7	0.5455763541545195
1105	8	0.6461476178506018
1105	9	0.7521105468800684
1105	10	0.712895599121353
1105	11	0.6367127587084482
1105	12	0.5607757528290236
1105	13	0.6378944021589206
1105	14	0.7729379833124881
1105	15	0.7853927267299241
1105	16	0.9076742631514918
1105	17	0.5887355972981254
1105	18	0.780527554326909
1105	19	0.8442282021684022
1105	20	0.868537044950557
1105	21	0.7773015816200086
1105	22	0.8586224203916757
1105	23	0.8819085681974173
1105	24	0.9010906083156911
1105	25	0.9072724187028527
1106	1	0.4495238639250587
1106	2	0.5069289321391537
1106	3	0.433621633575644
1106	4	0.35961447106025446
1106	5	0.468528263430251
1106	6	0.5558941761994234
1106	7	0.5003076136965987
1106	8	0.5785617290584639
1106	9	0.5574703570811081
1106	10	0.5899952380308591
1106	11	0.4896272958216912
1106	12	0.7069893893610057
1106	13	0.5562910856363601
1106	14	0.710062455600017
1106	15	0.678740896261111
1106	16	0.8620671696708868
1106	17	0.6783039785523783
1106	18	0.8432939523530862
1106	19	0.7360743724016672
1106	20	0.9024784171938975
1106	21	0.904789596637066
1106	22	0.8480246019782527
1106	23	0.888985424372585
1106	24	0.9520648808009184
1106	25	0.9137750691454869
1107	1	0.5063059190051042
1107	2	0.5596445239672825
1107	3	0.5396571588348138
1107	4	0.5856663854116846
1107	5	0.5857008881633803
1107	6	0.6121351763687244
1107	7	0.6124361347057388
1107	8	0.6262561856011288
1107	9	0.5211494143971152
1107	10	0.6602356867106636
1107	11	0.6917746225485928
1107	12	0.5836679531025553
1107	13	0.5991242598165272
1107	14	0.7902133533016809
1107	15	0.7564759711289568
1107	16	0.6523463090558437
1107	17	0.6146749825534528
1107	18	0.8480994189402438
1107	19	0.9662171385351712
1107	20	0.8915908216448827
1107	21	0.7789360135174377
1107	22	0.8064611749439763
1107	23	0.8315288257686433
1107	24	0.8419562240334812
1107	25	0.8357903668664619
1108	1	0.5686674354893348
1108	2	0.4567074443707675
1108	3	0.5362577317123325
1108	4	0.5414472948590259
1108	5	0.5216112245888951
1108	6	0.5513919862906462
1108	7	0.5087607617128711
1108	8	0.5499487874781579
1108	9	0.5854945517010961
1108	10	0.7174391775897959
1108	11	0.5859855071236311
1108	12	0.5998389876892247
1108	13	0.5950056172784528
1108	14	0.6463093673978619
1108	15	0.6951967631684468
1108	16	0.6505687548872174
1108	17	0.7080827330565143
1108	18	0.753891249308623
1108	19	0.6860539337474805
1108	20	0.6964383653426296
1108	21	0.8557697682377068
1108	22	0.8948903062379046
1108	23	0.8196822557816859
1108	24	0.8704308440820441
1108	25	0.8401842948832794
1109	1	0.2580256805758907
1109	2	0.19813395134534773
1109	3	0.22754277979815996
1109	4	0.3714700553710131
1109	5	0.28302570901909263
1109	6	0.05664973544102736
1109	7	0.25802909388577433
1109	8	0.39365303686562214
1109	9	0.4756332051636889
1109	10	0.35013931695331735
1109	11	0.3550585189612576
1109	12	0.33255853871160923
1109	13	0.3997408682416079
1109	14	0.5964954569359691
1109	15	0.5593619082603425
1109	16	0.40992443455083105
1109	17	0.3892494073295012
1109	18	0.42538697544829773
1109	19	0.5809998640080138
1109	20	0.5165583196947665
1109	21	0.473121515579237
1109	22	0.5408069127068771
1109	23	0.44391780513225143
1109	24	0.1783049142716635
1109	25	0.3054396017211758
1110	1	0.1653629698173127
1110	2	0.1632777419603606
1110	3	0.1893102858056821
1110	4	0.306003201679772
1110	5	0.15323203855457024
1110	6	0.27908934140187824
1110	7	0.33280448488557113
1110	8	0.4531085898488314
1110	9	0.5627034396583355
1110	10	0.603645099157184
1110	11	0.46975457720794367
1110	12	0.562295631354381
1110	13	0.1898302785323338
1110	14	0.5152896312773692
1110	15	0.5266525610346946
1110	16	0.4909293284705072
1110	17	0.479098764236511
1110	18	0.3264893144522034
1110	19	0.4792636553163234
1110	20	0.4490592451197486
1110	21	0.4608406617864844
1110	22	0.43971070193530437
1110	23	0.5684976498094159
1110	24	0.5117024186573786
1110	25	0.45754876596646454
1111	1	-0.28054833516906896
1111	2	-0.07615061142118407
1111	3	-0.04360128108775996
1111	4	-0.06024813135196894
1111	5	0.08736723773552058
1111	6	-0.1341289449740657
1111	7	0.20926749897216013
1111	8	0.15799315650543377
1111	9	-0.37279931537836575
1111	10	-0.1920348128219942
1111	11	0.036982297250595764
1111	12	0.007528421616953551
1111	13	0.048852788754849996
1111	14	0.23546330448002095
1111	15	0.37245064447652965
1111	16	0.16651188370595804
1111	17	0.33400369448025913
1111	18	0.297489937264686
1111	19	0.3417410204059905
1111	20	0.4166661038265289
1111	21	0.2469870314914786
1111	22	0.2297957653381104
1111	23	0.2663010766565179
1111	24	0.42462333400375485
1111	25	0.46968947396766225
1112	1	0.020026183214742588
1112	2	-0.1260681094739553
1112	3	-0.005179009596162132
1112	4	-0.21938191748648705
1112	5	0.01838635239682418
1112	6	-0.06825601977384821
1112	7	-0.07044539719238685
1112	8	0.026224034603750113
1112	9	-0.19212251235327216
1112	10	0.03498475550571811
1112	11	-0.08148332572333317
1112	12	0.052840016469836826
1112	13	-0.10264666631481845
1112	14	0.042336120419257384
1112	15	0.04340312464636753
1112	16	-0.1623299007092882
1112	17	0.009505753145027995
1112	18	0.03357998876822421
1112	19	0.03789858227893461
1112	20	0.18657591205465707
1112	21	0.2695527797159585
1112	22	0.15729346711753323
1112	23	0.3031789260168978
1112	24	0.266266713718764
1112	25	0.2810616173029743
1113	1	-0.2603824281603906
1113	2	-0.5636646139293617
1113	3	-0.13236459104160758
1113	4	-0.1948523855641966
1113	5	-0.08690710240126265
1113	6	-0.14885119960581505
1113	7	-0.03337389209135466
1113	8	0.14954364299894962
1113	9	0.043245311579518386
1113	10	-0.3457715459037445
1113	11	-0.05318639591452268
1113	12	-0.12082164459410905
1113	13	-0.044501239918972146
1113	14	-0.03515976808309773
1113	15	0.06909055256524965
1113	16	-0.23526979001535864
1113	17	-0.06909499602621594
1113	18	0.01901372893669162
1113	19	-0.011445877135493276
1113	20	0.09477698775219068
1113	21	-0.166460349362076
1113	22	-0.2837574512061707
1113	23	0.022161983056328438
1113	24	-0.2050809189971048
1113	25	-0.23948692671351068
1114	1	-0.5034999818042571
1114	2	-0.31032443164181345
1114	3	-0.35279931147632837
1114	4	-0.11983150001699272
1114	5	-0.3146069827327267
1114	6	-0.6445088873687652
1114	7	-0.410386242787915
1114	8	-0.8627156087339856
1114	9	-0.6152274468932433
1114	10	-0.6629108656746467
1114	11	-0.6560719882737942
1114	12	-0.6287841183460543
1114	13	-0.47497344144380094
1114	14	-1.5805946766759007
1114	15	-0.8399034037971305
1114	16	-0.7726564247704175
1114	17	-0.8807137576449762
1114	18	-1.003594217355463
1114	19	-0.8505360951730836
1114	20	-0.338784016861446
1114	21	-0.306768326512163
1114	22	-0.3847595811556701
1114	23	-0.2963610512561772
1114	24	-1.0675332209230888
1114	25	-0.8998194396776
1115	1	-1.5958159145402746
1115	2	-1.361217281458842
1115	3	-1.151246324062683
1115	4	-2.3864241685051546
1115	5	-1.594060873098499
1115	6	-1.4077421513704707
1115	7	-1.1720113412470354
1115	8	-1.191859814415075
1115	9	-1.6141097820973016
1115	10	-1.4282156171474298
1115	11	-1.252842683148998
1115	12	-1.6216604475441088
1115	13	-1.3770509800479829
1115	14	-2.09834372695569
1115	15	-2.0388659421551623
1115	16	-1.4850929904344454
1115	17	-1.5248814909520547
1115	18	-1.533438610929366
1115	19	-1.3458726202227211
1115	20	-1.2436861013229503
1115	21	-1.318796491972705
1115	22	-1.5135223090474952
1115	23	-1.9096023663417583
1115	24	-1.4577743842175295
1115	25	-1.3173886482304198
1116	1	-0.8274159933587839
1116	2	-0.917696331983425
1116	3	-0.7629094955338154
1116	4	-0.8834912444696457
1116	5	-0.9108051262098655
1116	6	-1.571202695187914
1116	7	-0.917369463388475
1116	8	-1.1026580259110759
1116	9	-1.1658270492042462
1116	10	-1.150971383715465
1116	11	-1.0070683922128225
1116	12	-1.6901839200191873
1116	13	-1.3075666231505068
1116	14	-1.553735263704695
1116	15	-1.4621057588239097
1116	16	-1.7401202564918208
1116	17	-1.5290896020932152
1116	18	-2.320403331320456
1116	19	-1.892851126283972
1116	20	-1.5773150250920338
1116	21	-1.6071407255055874
1116	22	-2.7890484427826294
1116	23	-2.6691324929761535
1116	24	-1.7751587854188813
1116	25	-2.1892899000419277
1201	1	0.6181854126435802
1201	2	0.6053555635455332
1201	3	0.5384228366454842
1201	4	0.5255701564757089
1201	5	0.6657549887306118
1201	6	0.621256168375659
1201	7	0.7313141369831371
1201	8	0.7330119862842963
1201	9	0.7553533479846877
1201	10	0.6573075343090338
1201	11	0.8120781324427426
1201	12	0.6921539850914016
1201	13	0.702075221870011
1201	14	0.7011931725610054
1201	15	0.7677150477728318
1201	16	0.8895629909310685
1201	17	0.788691461347554
1201	18	0.8910184117812818
1201	19	0.5633331519435245
1201	20	0.9193666408894217
1201	21	0.777129473790076
1201	22	1.0178206493198374
1201	23	0.9370635004557073
1201	24	0.8157088682127309
1201	25	0.8311787754578361
1202	1	0.7040259331684666
1202	2	0.7969150266614768
1202	3	0.7338968071346841
1202	4	0.8442853523977263
1202	5	0.9460769534831286
1202	6	0.9822371595900492
1202	7	0.8419527223170036
1202	8	0.8605152208798259
1202	9	1.1242483510931933
1202	10	1.0942033914396134
1202	11	1.0782512407302818
1202	12	1.0916216978044417
1202	13	1.0715495252919283
1202	14	1.0489094475090752
1202	15	1.2576363852320185
1202	16	1.068372293917875
1202	17	1.282120809444912
1202	18	1.3126787425345157
1202	19	1.4328377041581675
1202	20	1.5381833760633938
1202	21	1.4226213975584159
1202	22	1.3811948230966067
1202	23	1.2997015767213398
1202	24	1.1387680651654293
1202	25	1.3397483956349134
1203	1	0.5077195182731806
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2211	7	0.10909623994651696
2211	8	0.0232660814888348
2211	9	-0.17529771096551627
2211	10	-0.30869174597604143
2211	11	-0.10817322114522909
2211	12	-0.215506317514766
2211	13	-0.11729100441326779
2211	14	-1.2420779570869485
2211	15	-0.7043477625782586
2211	16	-0.36876467749835484
2211	17	-0.1327796292049328
2211	18	-0.39847922317679263
2211	19	-0.26632981489330376
2211	20	-0.385268898420577
2211	21	-0.4967984018933578
2211	22	-0.26650446472537226
2211	23	-0.38534255730287725
2211	24	-0.4162936979871148
2211	25	-0.4818852709254102
2212	1	-0.05352775438396051
2212	2	-0.010715475504667893
2212	3	0.029179848692741928
2212	4	0.22553612279870094
2212	5	0.07827625658968174
2212	6	0.09822271663880144
2212	7	0.05023832837078146
2212	8	-0.15829308532036634
2212	9	-0.16899857707386445
2212	10	-0.13350608997907187
2212	11	-0.09823521975453531
2212	12	0.05465748349317323
2212	13	0.04093341496750469
2212	14	-0.07275152194847578
2212	15	-0.01549368250313421
2212	16	0.04376756598591669
2212	17	-0.00481634688109267
2212	18	0.11048945088250406
2212	19	0.06841549736901698
2212	20	-0.09725762399973803
2212	21	-0.0997036951151884
2212	22	-0.008891131103247574
2212	23	-0.4554251598751833
2212	24	-0.18362557440416793
2212	25	-0.3794964460608412
2213	1	-0.3122377256332811
2213	2	-0.3783188966951023
2213	3	-0.29804120651538213
2213	4	-0.396510562446565
2213	5	-0.3768461996372565
2213	6	-0.09358524567433335
2213	7	-0.7222302876441304
2213	8	-0.44970218963537434
2213	9	-0.5584353606893941
2213	10	-0.26914539635136947
2213	11	-0.3751092303840622
2213	12	-0.5439272026067883
2213	13	-0.39593471712110073
2213	14	-0.3549001687805813
2213	15	-0.4905091312284142
2213	16	-0.8156339865106546
2213	17	-0.7163011198550535
2213	18	-0.8353768113084428
2213	19	-1.0533573241099248
2213	20	-0.512835857748982
2213	21	-1.3270903726962757
2213	22	-0.743113117247546
2213	23	-0.8476136222856283
2213	24	-1.045491148424631
2213	25	-0.9267891071137981
2214	1	-1.009356968955089
2214	2	-0.8319584975081469
2214	3	-0.7414467274035559
2214	4	-1.3434393635313313
2214	5	-1.2768564496587302
2214	6	-2.6913996986789037
2214	7	-1.36275248276133
2214	8	-1.959129442677046
2214	9	-1.4683049983340055
2214	10	-1.6257702566322436
2214	11	-1.321418550736496
2214	12	-1.44487359051503
2214	13	-1.539195301651631
2214	14	-1.6947829101158902
2214	15	-2.157855026503235
2214	16	-1.5870938161758517
2214	17	-1.6494089922404456
2214	18	-1.7876836458330487
2214	19	-3.3595607082506973
2214	20	-2.02370061701577
2214	21	-2.3049999954375195
2214	22	-2.245901900403993
2214	23	-2.160424647198184
2214	24	-1.4235932950268122
2214	25	-1.2046246345841212
2215	1	-1.3682579109698452
2215	2	-1.2252042623260913
2215	3	-1.2007261312086293
2215	4	-1.0793647528766854
2215	5	-1.8181451820805492
2215	6	-1.5165711979980685
2215	7	-1.759657397025446
2215	8	-2.3919933344679976
2215	9	-1.956932058903078
2215	10	-1.4301049868522142
2215	11	-1.9636786119693994
2215	12	-2.788978327040006
2215	13	-2.2193288936368134
2215	14	-2.0850313430370377
2215	15	-3.257770054721206
2215	16	-2.7967792283810624
2215	17	-2.454146605909692
2215	18	-3.096423078290897
2215	19	-3.8169916868226714
2215	20	-3.1454708122962955
2215	21	-2.2468550764799247
2215	22	-3.273628144493969
2215	23	-3.1854842665563226
2215	24	-2.2128165021349773
2215	25	-2.548272859470906
2216	1	-1.0932552192752318
2216	2	-0.9927411122818448
2216	3	-1.3767445765383428
2216	4	-0.9301811898083088
2216	5	-0.7516940698198127
2216	6	-1.1672773411016877
2216	7	-2.575376105747324
2216	8	-1.5618185187505986
2216	9	-1.7478351815314355
2216	10	-1.7119121512241549
2216	11	-2.108324240526052
2216	12	-1.589395999262507
2216	13	-2.0100534453055303
2216	14	-2.1440474695703173
2216	15	-2.0980417451842044
2216	16	-1.8966128685238175
2216	17	-1.9395929413972581
2216	18	-1.7994690261744353
2216	19	-2.092062393044557
2216	20	-2.5624358412746915
2216	21	-2.375278715073314
2216	22	-2.877064322445186
2216	23	-1.8086797150976892
2216	24	-2.0763173857986246
2216	25	-1.8172180565686062
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60877.0
3	2.0
4	947.0
5	8774.0
6	10250.0
7	8104.0
8	6494.0
9	5962.0
10	5047.0
11	4528.0
12	4297.0
13	4016.0
14	4013.0
15	4104.0
16	4078.0
17	4314.0
18	4647.0
19	5659.0
20	7147.0
21	9347.0
22	12037.0
23	14549.0
24	17188.0
25	19967.0
26	22494.0
27	25335.0
28	28686.0
29	34411.0
30	43584.0
31	55530.0
32	70144.0
33	91373.0
34	118354.0
35	155732.0
36	213894.0
37	331879.0
38	1116971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.583599024441476	19.37412321934313	13.717952716919434	27.32432503929596
2	13.514312147029175	20.416138176180155	40.259474856345925	25.81007482044474
3	17.124946000272217	24.28612853020138	30.177079655269683	28.41184581425672
4	11.481405039398293	17.133670061440583	37.36103454113725	34.02389035802388
5	12.699793277817871	38.075138538605245	36.10954064720309	13.115527536373786
6	32.30552137017379	37.2130550123403	17.674757053474096	12.806666564011815
7	27.93428313992657	31.40447529164935	22.197397642020082	18.463843926404
8	25.02386432273881	37.43295684683013	20.3047685219911	17.238410308439956
9	25.99921139039609	14.930653271091854	20.034749344108103	39.035385994403946
10	14.515804801411663	29.164150586462256	34.74959193095547	21.57045268117061
11	34.19759379534714	22.159034866847573	24.844177456606804	18.799193881198477
12	23.17557754291105	24.610187387033353	31.851100439655045	20.363134630400552
13	28.90703043750661	21.74548929313716	25.780585456846666	23.566894812509567
14	20.529394242472662	22.18460937546235	27.477683811417602	29.808312570647384
15	22.82291819961911	31.24599295272582	23.125017507714244	22.80607133994083
16	22.016661359621555	28.079832163218665	27.986791179020244	21.916715298139536
17	21.62660978925498	28.742928963085586	27.933846466503955	21.696614781155475
18	21.5555602021698	27.539579619055658	29.809078072719426	21.095782106055115
19	23.367548188737857	26.967387063162885	27.555866268929	22.10919847917026
20	23.535209111554042	27.49515905292611	27.860261939819175	21.109369895700674
21	22.610810618882486	26.81638675519681	27.26984728156451	23.302955344356207
22	22.985819385748687	28.119821929611383	27.091940437001526	21.802418247638407
23	21.961664631608432	27.84598784488319	27.744162604769336	22.448184918739038
24	23.050860563672558	27.047058981336896	28.179209337153328	21.722871117837215
25	22.56476982389804	27.3028657007545	27.860769142151952	22.271595333195513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	123.0
1	123.0
2	291.0
3	459.0
4	459.0
5	459.0
6	1147.5
7	1836.0
8	1836.0
9	1836.0
10	2695.0
11	3554.0
12	3554.0
13	3554.0
14	6425.0
15	9296.0
16	9296.0
17	9296.0
18	17640.5
19	25985.0
20	25985.0
21	25985.0
22	43080.0
23	60175.0
24	60175.0
25	60175.0
26	88961.0
27	117747.0
28	117747.0
29	117747.0
30	154994.5
31	192242.0
32	192242.0
33	192242.0
34	218400.0
35	244558.0
36	244558.0
37	244558.0
38	265201.0
39	285844.0
40	285844.0
41	285844.0
42	301006.0
43	316168.0
44	316168.0
45	316168.0
46	328845.0
47	341522.0
48	341522.0
49	341522.0
50	326361.5
51	311201.0
52	311201.0
53	311201.0
54	276023.0
55	240845.0
56	240845.0
57	240845.0
58	206713.0
59	172581.0
60	172581.0
61	172581.0
62	140655.0
63	108729.0
64	108729.0
65	108729.0
66	84005.0
67	59281.0
68	59281.0
69	59281.0
70	43007.5
71	26734.0
72	26734.0
73	26734.0
74	18533.5
75	10333.0
76	10333.0
77	10333.0
78	7070.0
79	3807.0
80	3807.0
81	3807.0
82	2528.5
83	1250.0
84	1250.0
85	1250.0
86	809.5
87	369.0
88	369.0
89	369.0
90	228.0
91	87.0
92	87.0
93	87.0
94	47.0
95	7.0
96	7.0
97	7.0
98	4.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08016617121710948
2	0.0
3	0.0
4	0.029273277088137418
5	0.07365661499131074
6	0.07795686728592931
7	0.03771597425371883
8	0.0656873400966965
9	0.04584305657198879
10	0.10604658869664876
11	0.004773674565585751
12	0.009784060266655095
13	0.0039057337354792515
14	0.0055232598279504564
15	0.0054443561161225925
16	0.014518282976326912
17	0.024854669225777053
18	0.0013413631010736823
19	0.0026038224903195007
20	0.004221348582790706
21	1.9725927956965917E-4
22	2.761629913975228E-4
23	0.0
24	3.945185591393183E-5
25	0.0026432743462334327
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2534735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.93905188041317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25849543042143	52.2623004215005
2	13.363578220055466	17.62363355120394
3	3.8573994379353187	7.630597849844809
4	1.4813260252355451	3.9070893451925137
5	0.6739588221135187	2.2220102868302734
6	0.3815302747040799	1.509464674659637
7	0.23486976404839788	1.0840962689709397
8	0.14410796335094653	0.7601873977422995
9	0.09833329826886063	0.583560401051029
>10	0.4606914708934336	5.170225244339014
>50	0.0238901998922613	1.0929548970996026
>100	0.017450904754161438	2.31162184794036
>500	0.002513204242734903	1.2044169663089046
>1k	0.0017951458876271041	2.4387026842082786
>5k	5.9838196254236805E-5	0.1991381631077254
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5047	0.19911351679761397	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4895	0.1931168346986963	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4223	0.16660518752453413	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4191	0.1653427281352883	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4167	0.16439588359335394	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3610	0.1424211998492939	No Hit
CCCATGTACTCTGCGTTGATACCAC	3559	0.1404091551976834	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3008	0.11867118258910696	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2688	0.10604658869664876	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2571	0.10143072155471874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.945185591393183E-5
2	0.0	0.0	0.0	0.0	3.945185591393183E-5
3	0.0	0.0	0.0	0.0	3.945185591393183E-5
4	0.0	0.0	0.0	3.945185591393183E-5	3.945185591393183E-5
5	0.0	0.0	0.0	3.945185591393183E-5	3.945185591393183E-5
6	0.0	0.0	0.0	3.945185591393183E-5	3.945185591393183E-5
7	0.0	0.0	0.0	7.890371182786366E-5	3.945185591393183E-5
8	3.945185591393183E-5	0.0	0.0	7.890371182786366E-5	3.945185591393183E-5
9	3.945185591393183E-5	0.0	0.0	2.761629913975228E-4	3.945185591393183E-5
10	3.945185591393183E-5	0.0	0.0	2.761629913975228E-4	3.945185591393183E-5
11	3.945185591393183E-5	0.0	0.0	3.1561484731145465E-4	3.945185591393183E-5
12	3.945185591393183E-5	3.945185591393183E-5	0.0	3.1561484731145465E-4	1.5780742365572732E-4
13	3.945185591393183E-5	3.945185591393183E-5	0.0	3.1561484731145465E-4	2.36711135483591E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	905	0.0	12.825612	4
AACCGTG	65	7.989395E-4	11.698765	7
GGCGAGG	1070	0.0	11.175649	19
TGCACCG	120	1.2505006E-7	11.099761	5
CCGGTTT	215	0.0	11.037782	12
GATATAC	655	0.0	10.884729	1
AGGACCG	140	8.167262E-9	10.873237	5
GCGTATT	70	0.0015015891	10.848776	11
GTCCTAC	2515	0.0	10.734406	1
TGTAGGA	2515	0.0	10.47024	2
AGGACCT	1800	0.0	10.306922	5
GCACCGC	120	1.5001315E-6	10.30468	6
TTAGACT	130	3.766654E-7	10.245934	4
GGACCTG	1800	0.0	10.198991	6
GAACCGT	75	0.0026209366	10.146146	6
TGCGTAT	75	0.0026477114	10.133722	10
GCCGGTT	235	0.0	10.098595	11
AGGACGT	2420	0.0	10.064483	5
GTAGGAC	2560	0.0	10.063984	3
TTAGGAC	1560	0.0	10.055414	3
>>END_MODULE