FastQCFastQC Report
Thu 26 May 2016
SRR1512938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536958
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44370.28868713393599565No Hit
CCCATGTACTCTGCGTTGATACCAC37480.24385832273881264No Hit
GTCCTACAGTGGACATTTCTAAATT35860.23331802170260996No Hit
GTCCTAAAGTGTGTATTTCTCATTT34920.22720204455814666No Hit
CTGTAGGACGTGGAATATGGCAAGA33590.21854858753459755No Hit
CTTTAGGACGTGAAATATGGCGAGG30890.20098141914092643No Hit
GTATCAACGCAGAGTACTTTTTTTT29600.19258821646395022No Hit
GTCCTACAGTGTGCATTTCTCATTT20650.13435630641826257No Hit
GAGTACATGGGAAGCAGTGGTATCA18660.12140865267626051No Hit
GGTATCAACGCAGAGTACTTTTTTT18280.1189362363838179No Hit
TATCAACGCAGAGTACTTTTTTTTT16680.10852606252090168No Hit
CTGAAGGACCTGGAATATGGCGAGA16350.10637896416167521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG250.00604878218.9908899
TAGGACC5850.014.4493524
GGCGAGG8400.012.32106819
ATGTCGA752.0698605E-411.40232517
TGTCGAG752.0698605E-411.40232518
TGTAGGA19750.011.3971292
AGGACGT17900.011.3012545
TAGGACA6250.011.2451684
GGACGTG18050.011.2040566
CTAGGAC1701.0913936E-1111.1736563
GTAGGAC19600.011.0966953
GTAATAC1201.2793862E-711.0805423
TCGAACT1201.2840064E-711.07765719
CTGTAGG19700.010.9807581
AATGTCC5050.010.9056588
GGACCTG11350.010.8747786
TAGCACC1053.4759978E-610.8544084
CGTGCGC700.001497365110.85193510
AGGACCT11600.010.8072715
AAATGTC4850.010.7669797
Produced by FastQC (version 0.11.5)