##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512938_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1536958
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.686878886736007	31.0	30.0	34.0	22.0	34.0
2	28.828893827938042	31.0	30.0	34.0	16.0	34.0
3	29.0578285158085	31.0	30.0	34.0	22.0	34.0
4	32.432193332543896	35.0	33.0	37.0	23.0	37.0
5	32.57332471023932	35.0	33.0	37.0	25.0	37.0
6	32.590068173626086	36.0	33.0	37.0	25.0	37.0
7	32.39457096420332	36.0	33.0	37.0	23.0	37.0
8	32.38018085074543	36.0	33.0	37.0	23.0	37.0
9	33.65047776191672	38.0	34.0	39.0	19.0	39.0
10	33.52959612429227	38.0	34.0	39.0	19.0	39.0
11	33.57586479266187	38.0	34.0	39.0	19.0	39.0
12	33.4699276102535	38.0	34.0	39.0	18.0	39.0
13	33.46410832306413	38.0	34.0	39.0	18.0	39.0
14	34.42528032646305	39.0	34.0	41.0	17.0	41.0
15	34.36837376167729	39.0	34.0	41.0	17.0	41.0
16	34.264746336594754	39.0	34.0	41.0	17.0	41.0
17	34.20653459626092	39.0	33.0	41.0	17.0	41.0
18	33.9047085216382	39.0	33.0	41.0	11.0	41.0
19	34.13178304156652	39.0	34.0	41.0	17.0	41.0
20	34.21346126569497	39.0	34.0	41.0	16.0	41.0
21	34.13933171888887	39.0	34.0	41.0	11.0	41.0
22	34.056256579555196	39.0	34.0	41.0	10.0	41.0
23	33.794092616714316	39.0	34.0	41.0	2.0	41.0
24	33.79176984667115	39.0	34.0	41.0	2.0	41.0
25	33.69895013396592	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4875194930353146
1101	2	0.726296002871436
1101	3	0.9074494612349326
1101	4	0.834954161139116
1101	5	0.6445402694458906
1101	6	0.8564455741664929
1101	7	0.8186863648620744
1101	8	0.6675511158781404
1101	9	0.4664167817294711
1101	10	0.5057298761992683
1101	11	0.7393348561858772
1101	12	0.919315224427919
1101	13	0.7974013140616663
1101	14	0.7649761437633558
1101	15	0.5297995712749142
1101	16	0.895364066638912
1101	17	0.9270607416843575
1101	18	1.159688154832935
1101	19	0.7761034135518088
1101	20	0.9855909357238133
1101	21	0.8136893724532968
1101	22	0.8026631417359411
1101	23	0.9293561554850598
1101	24	0.7796459427600837
1101	25	1.1661820823887936
1102	1	0.36111363484756254
1102	2	0.3973227186588808
1102	3	0.39410923519599095
1102	4	0.6121205177841489
1102	5	0.5662798848758754
1102	6	0.47862166795625427
1102	7	0.6557837517252523
1102	8	0.7612599833052229
1102	9	0.5679262773292635
1102	10	0.7466488173923906
1102	11	0.6222592663927387
1102	12	0.6639335531240604
1102	13	0.6553831139238895
1102	14	0.6116546108251697
1102	15	0.8553482697296673
1102	16	0.7911143481526395
1102	17	0.6162604008713046
1102	18	0.9061664401254745
1102	19	0.9675315390817261
1102	20	1.0130666067190077
1102	21	0.7498799395391273
1102	22	0.9992782909421223
1102	23	1.3091671563194254
1102	24	1.1392868592416576
1102	25	1.0617113446608641
1103	1	0.1105428792576788
1103	2	0.3176014887635574
1103	3	0.31947368065958415
1103	4	0.4784830831905609
1103	5	0.09105168321359258
1103	6	0.3530767262755674
1103	7	0.30284810680663554
1103	8	0.30899979116927767
1103	9	0.6329327408639358
1103	10	0.5303624533049671
1103	11	0.5586667010667838
1103	12	0.6345338572588517
1103	13	0.6627850009541802
1103	14	0.8796800948527306
1103	15	0.8602891190005693
1103	16	1.0554799562478863
1103	17	0.7897504883301707
1103	18	1.0824957223512897
1103	19	1.1978241280089676
1103	20	1.018064615360295
1103	21	0.8009732088807908
1103	22	0.9956534481722912
1103	23	1.1791840661061812
1103	24	0.9802247355548204
1103	25	1.0414162480967235
1104	1	0.2501373534109774
1104	2	0.32211780577987525
1104	3	0.3375760847914009
1104	4	0.46859060638632144
1104	5	0.33170196430504717
1104	6	0.31621203867558734
1104	7	0.379762613868202
1104	8	0.36250143658268996
1104	9	0.348681861024815
1104	10	0.5238379320745636
1104	11	0.5420097469098266
1104	12	0.38566507897830604
1104	13	0.38655551148939793
1104	14	0.44749626561007716
1104	15	0.5439698173871506
1104	16	0.4167453248661914
1104	17	0.5226878940616899
1104	18	0.6501204995348857
1104	19	0.5159202475756786
1104	20	0.46060369392878897
1104	21	0.529751375158952
1104	22	0.4731289955007796
1104	23	0.7514165721622135
1104	24	0.6210304168016734
1104	25	0.4591570040727788
1105	1	0.5453770401947828
1105	2	0.602965634421821
1105	3	0.622374007456969
1105	4	0.6963922165408718
1105	5	0.4005595772803119
1105	6	0.34557789340909295
1105	7	0.6180537345122588
1105	8	0.5523391723763069
1105	9	0.5275140582393689
1105	10	0.6788483628202897
1105	11	0.7200229102171107
1105	12	0.6049311070678627
1105	13	0.5818602067940901
1105	14	0.6588584328545934
1105	15	0.8063728555254883
1105	16	0.8428229237379128
1105	17	0.7707394748338601
1105	18	0.7602713780681185
1105	19	0.7899348359284986
1105	20	0.7807890233052959
1105	21	0.8822242699112621
1105	22	0.5854431519325871
1105	23	0.8775569758761463
1105	24	1.0049387878423985
1105	25	0.9546266079982644
1106	1	0.7008179958189622
1106	2	0.8009356204618392
1106	3	0.8374595123429636
1106	4	0.9532241468255691
1106	5	0.950503525839892
1106	6	0.7988264974109498
1106	7	0.6468356203788375
1106	8	0.7523198716444668
1106	9	0.9627878642379315
1106	10	1.1117690658957144
1106	11	0.9944528776438233
1106	12	1.2605637480425216
1106	13	1.2703992735904848
1106	14	1.3442353523520367
1106	15	1.4651649998632337
1106	16	1.4349614448259445
1106	17	1.328259621625179
1106	18	1.475622598508913
1106	19	0.811415492893687
1106	20	1.155147868857135
1106	21	0.9463727639253889
1106	22	1.0791717360520465
1106	23	1.1620405094435569
1106	24	1.321612240611131
1106	25	1.3010152559952175
1107	1	0.3978911285293556
1107	2	0.6393056710371887
1107	3	0.662960210901101
1107	4	0.8344721982331862
1107	5	0.7054826277980766
1107	6	0.6300746466558422
1107	7	0.8075954703095007
1107	8	0.687900059726843
1107	9	0.9118717160001069
1107	10	0.7364496271946805
1107	11	0.7507597944782276
1107	12	0.8720659545991865
1107	13	0.8336766722669466
1107	14	0.9987949280774302
1107	15	1.1415357653029332
1107	16	1.0121439661316387
1107	17	1.0831196882327987
1107	18	1.4157686891271055
1107	19	1.267359531277208
1107	20	1.096115629518465
1107	21	1.12646199804216
1107	22	1.0872596174054365
1107	23	1.1941958711218703
1107	24	1.1508263529204896
1107	25	1.2379612524287253
1108	1	0.25646658942310907
1108	2	0.37272300630845123
1108	3	0.2679276146713967
1108	4	0.37333831653306504
1108	5	0.34737658792497683
1108	6	0.4835270059198251
1108	7	0.6550107654572699
1108	8	0.5556592866307923
1108	9	0.6040668391258137
1108	10	0.6211905146564334
1108	11	0.48365016472612865
1108	12	0.5737063198310537
1108	13	0.5529218105210276
1108	14	0.6900780148757804
1108	15	0.5795793334163335
1108	16	0.42593622826905175
1108	17	0.4901105221232456
1108	18	0.875703215769498
1108	19	0.8688200414043905
1108	20	0.6814811543205721
1108	21	0.5783159662910364
1108	22	0.43802233627529574
1108	23	0.6073446356112058
1108	24	0.6132428960588285
1108	25	0.7524900296467578
1109	1	0.08181621518164306
1109	2	-0.06430315170534584
1109	3	0.3517557167881016
1109	4	0.4507650113760917
1109	5	0.258209153137976
1109	6	0.2001144334586371
1109	7	0.3721262515905579
1109	8	0.44893336575518106
1109	9	0.5830138330310533
1109	10	0.6027044922400009
1109	11	0.15747129997763665
1109	12	0.3762883625291664
1109	13	0.42012309997027586
1109	14	0.30687659965888514
1109	15	0.6375932969337867
1109	16	0.5635223832379452
1109	17	0.3897174982555285
1109	18	-1.2125863278098166
1109	19	0.14314358527164472
1109	20	0.4079496156031652
1109	21	0.4902468470451069
1109	22	0.6655985456717914
1109	23	0.29482810792329417
1109	24	0.5929600512610591
1109	25	0.713848419423833
1110	1	0.5784690101060583
1110	2	0.5417739795420466
1110	3	0.6841438094371668
1110	4	0.43344506468282873
1110	5	0.5920743001811744
1110	6	0.4157812173129116
1110	7	0.697410813077262
1110	8	0.5216308336852933
1110	9	0.9164754186611219
1110	10	1.0631826176743502
1110	11	0.9951714250685981
1110	12	0.286205377352033
1110	13	0.6692280864391975
1110	14	0.795817414467038
1110	15	0.9510954349262803
1110	16	1.0546137813951049
1110	17	0.5087870831661263
1110	18	1.0718031383870112
1110	19	0.9770563159336447
1110	20	0.9132174982956514
1110	21	0.9144781917320159
1110	22	1.1192826787424082
1110	23	0.8728943488991661
1110	24	1.183614530416655
1110	25	1.1233116729333261
1111	1	0.11710782148824705
1111	2	0.3712765182041444
1111	3	0.44113450724697145
1111	4	0.3495146086384793
1111	5	0.386488495065592
1111	6	0.5742539448880635
1111	7	0.49825794860979755
1111	8	0.2395328716841405
1111	9	0.6248310891714794
1111	10	0.6612097383200535
1111	11	0.39946432138889065
1111	12	0.5727703866459066
1111	13	0.6429853479276346
1111	14	0.553845533112586
1111	15	0.746863250263047
1111	16	0.6748395788593555
1111	17	0.6633983830193344
1111	18	0.8345529606182893
1111	19	0.5644407662644397
1111	20	0.49899051154825713
1111	21	0.7005013702783458
1111	22	0.5718766050817052
1111	23	0.7799823932768959
1111	24	0.9766549785185816
1111	25	0.36541163093416884
1112	1	0.18031577601456306
1112	2	0.543056728734701
1112	3	0.617632895427203
1112	4	0.5460751916889137
1112	5	0.6899769602280799
1112	6	0.5801291394966128
1112	7	0.2626369313897925
1112	8	0.5458213387217441
1112	9	0.8275585517780755
1112	10	0.8235567187471275
1112	11	0.7358352293572494
1112	12	0.8072109479380316
1112	13	0.5546970020781785
1112	14	0.8071044613197245
1112	15	0.759489215188971
1112	16	1.0878182395011038
1112	17	1.057589755082759
1112	18	0.9977980876132264
1112	19	0.8924587201674541
1112	20	0.873293137112114
1112	21	0.8716667853330051
1112	22	0.8313548466867786
1112	23	1.0261266394700996
1112	24	0.72562657205075
1112	25	1.0342500772326275
1113	1	-0.21693985802328086
1113	2	-0.125232039996785
1113	3	-0.26269659054967676
1113	4	0.06119892146385553
1113	5	-0.14901003914977196
1113	6	-0.07749104346798674
1113	7	-0.264912136825707
1113	8	0.056935593397149376
1113	9	-0.1733882371396689
1113	10	-0.4616114680465131
1113	11	-0.21643888380282306
1113	12	0.015079098611202824
1113	13	-0.10237416348679318
1113	14	-0.062071799703545594
1113	15	-0.4228819013455478
1113	16	-0.5101482330123162
1113	17	-0.3837927661809388
1113	18	-0.2632675829708404
1113	19	-0.3934972842768758
1113	20	-0.41855596310364973
1113	21	-0.386944787775235
1113	22	-0.22386672423775877
1113	23	-0.6029908193518807
1113	24	-0.026934135745641186
1113	25	-0.054877695837433293
1114	1	-1.4729173343495923
1114	2	-1.0237681048119178
1114	3	-0.9157886744557118
1114	4	-1.2213846012359468
1114	5	-1.329789803069069
1114	6	-1.9155688989877007
1114	7	-1.8930891766306495
1114	8	-1.417411178624043
1114	9	-1.3782595600342518
1114	10	-1.4133584168864886
1114	11	-2.06178582159162
1114	12	-1.6525641004273446
1114	13	-1.5464628215289373
1114	14	-1.8733215493253894
1114	15	-1.8317447700921434
1114	16	-2.4693787259637396
1114	17	-4.525055777211392
1114	18	-3.4891437338469906
1114	19	-2.3169969147532505
1114	20	-1.4804326941664243
1114	21	-2.456373787436796
1114	22	-2.529772713283279
1114	23	-2.444854565285393
1114	24	-1.842293540639929
1114	25	-1.7838816742600194
1115	1	-1.0946741770903294
1115	2	-0.7587678378090388
1115	3	-0.9010941031220483
1115	4	-0.8307012820371753
1115	5	-0.690449870390001
1115	6	-0.8543121028593355
1115	7	-2.1553491795088497
1115	8	-1.536337157280105
1115	9	-1.2458630598536828
1115	10	-0.8522088744570127
1115	11	-1.596150359264822
1115	12	-1.2418778306204814
1115	13	-1.0968144054895745
1115	14	-1.0668863273916926
1115	15	-2.527654376493693
1115	16	-1.1919616484595892
1115	17	-1.3560561691173092
1115	18	-1.423733096995342
1115	19	-1.3924588071067419
1115	20	-1.1225615850545694
1115	21	-1.8828697050080265
1115	22	-2.0841385723944583
1115	23	-1.378892182823229
1115	24	-1.3259139548654844
1115	25	-1.774259071515953
1116	1	-0.7525586632984016
1116	2	-1.5619779746007367
1116	3	-1.2845685133186535
1116	4	-0.7629679062541541
1116	5	-1.3471679952395057
1116	6	-1.184707524082004
1116	7	-0.7538808867298386
1116	8	-1.384671791037011
1116	9	-1.4602701450919255
1116	10	-2.219728072286614
1116	11	-0.9131719189610976
1116	12	-0.9792296536856924
1116	13	-1.280588978369778
1116	14	-1.155710885177328
1116	15	-0.7545652253225654
1116	16	-0.7292524501033952
1116	17	-0.9223407886238277
1116	18	-0.653568994394135
1116	19	-1.3196314691883586
1116	20	-1.6958530154039195
1116	21	-2.113071610339606
1116	22	-1.304098285392783
1116	23	-1.0786939235014827
1116	24	-1.7696745547245456
1116	25	-1.530040127620687
1201	1	-0.06712034145582635
1201	2	-0.0899805323146694
1201	3	0.10521072718885094
1201	4	0.0644274071567068
1201	5	0.05315697987771273
1201	6	0.14285219169145336
1201	7	0.1337638308675153
1201	8	-0.3597497467690616
1201	9	-0.002884978208058442
1201	10	0.11959511606704609
1201	11	-0.31590084344858127
1201	12	-0.9860827178169274
1201	13	-0.6135733918653159
1201	14	-0.00693545068122603
1201	15	-0.4263198549898064
1201	16	0.21350264724021173
1201	17	0.327073262014423
1201	18	0.5303541179089208
1201	19	0.02036267421924265
1201	20	0.0424014028612234
1201	21	0.07479077598583217
1201	22	0.20599195796526004
1201	23	0.6099223927173725
1201	24	0.19695544054453507
1201	25	0.1898722091712841
1202	1	0.3760449048943926
1202	2	0.37635346035660433
1202	3	0.06616484045002835
1202	4	0.4842489086441901
1202	5	0.4554255973362018
1202	6	0.44213151587890565
1202	7	0.562282229577832
1202	8	0.4561411814125975
1202	9	0.8002522683754947
1202	10	0.8165983986154473
1202	11	0.4022401417401795
1202	12	0.5618271102072327
1202	13	0.6223246423177358
1202	14	0.7611596636776738
1202	15	0.3726704564545713
1202	16	0.4820829848283097
1202	17	0.6815725805807062
1202	18	0.9432817374514855
1202	19	0.744066491838943
1202	20	0.5651591677586367
1202	21	0.7671380367283547
1202	22	0.818852759882823
1202	23	0.9044237264183792
1202	24	0.9778996902515473
1202	25	0.7026338452485561
1203	1	0.08863246090806598
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2211	11	-0.09517659615886487
2211	12	-0.026510980256574612
2211	13	-0.1477924934468433
2211	14	0.05218569382891758
2211	15	0.12315533774914655
2211	16	0.0930102364252221
2211	17	0.008496203399410263
2211	18	0.14374452257067816
2211	19	-0.10686872815447401
2211	20	-0.259676026350931
2211	21	-0.1292366955938249
2211	22	-0.2315192973828033
2211	23	0.3355383327545667
2211	24	0.15167624156514847
2211	25	0.15928041007853722
2212	1	0.21925001689393397
2212	2	0.306123194042101
2212	3	0.302833894004781
2212	4	0.21244180714475647
2212	5	0.2130266887749741
2212	6	0.014301845712864747
2212	7	0.07152044608270103
2212	8	0.21782689575503866
2212	9	0.385489652337327
2212	10	0.43345670018794635
2212	11	0.16370200259960654
2212	12	0.404721673065886
2212	13	0.12439425297481677
2212	14	0.23416521683236624
2212	15	0.2040646086669966
2212	16	0.2917102398253846
2212	17	0.3276135671111149
2212	18	-2.538533848310042
2212	19	-0.6899591052194936
2212	20	-0.03513993226101775
2212	21	-0.15860812500338284
2212	22	0.18783427782452122
2212	23	0.1375465304868584
2212	24	-0.3523849560798027
2212	25	-0.010270174716588087
2213	1	0.37155395451072337
2213	2	0.6093262210504875
2213	3	0.1614689783630361
2213	4	0.13681173052646756
2213	5	0.12065598129192523
2213	6	0.19472363025744954
2213	7	0.2684029696495287
2213	8	-8.961766158677165E-5
2213	9	0.2534015850932292
2213	10	0.26533466330285194
2213	11	0.2310370415554317
2213	12	0.021263112551984875
2213	13	0.33222455566217945
2213	14	0.2906420690023239
2213	15	0.10570906586543316
2213	16	-0.2843098665806636
2213	17	0.16005939742996844
2213	18	0.23514412585233657
2213	19	0.4283324161122408
2213	20	-0.18519867249848687
2213	21	0.054181079726546955
2213	22	0.29090082808851747
2213	23	-0.3111322183460885
2213	24	-0.024700655216619793
2213	25	-0.16744476929443408
2214	1	-1.7944189086070743
2214	2	-3.7913835094351995
2214	3	-2.008504164021595
2214	4	-1.6289193922353071
2214	5	-1.6639692884296586
2214	6	-1.7284714441737101
2214	7	-1.905539907191617
2214	8	-1.6081397024234327
2214	9	-1.8745102625841632
2214	10	-1.8408870963836712
2214	11	-1.549224683833632
2214	12	-2.108454151592987
2214	13	-2.068990255079992
2214	14	-3.2373107959917995
2214	15	-3.4184278796098937
2214	16	-2.598777415992352
2214	17	-3.104262300512403
2214	18	-2.6150204888576063
2214	19	-4.784723090353442
2214	20	-2.9133433152660224
2214	21	-2.875886577028325
2214	22	-3.0399758297856074
2214	23	-2.959981442616993
2214	24	-3.313111299646664
2214	25	-3.1550610304787448
2215	1	-0.9912468839537816
2215	2	-2.7760430321196985
2215	3	-1.3545067415574863
2215	4	-0.9121798946593636
2215	5	-0.9492606638014287
2215	6	-1.4917347747593581
2215	7	-1.8764937679196052
2215	8	-1.7289453145997022
2215	9	-1.4556836737264334
2215	10	-2.9508692421765517
2215	11	-1.9193770021788588
2215	12	-2.2740487610710076
2215	13	-1.6488996159499614
2215	14	-2.99709238374011
2215	15	-2.5766853641785943
2215	16	-2.340877820327485
2215	17	-2.6096932904954215
2215	18	-1.4824262714133667
2215	19	-2.2162562873371954
2215	20	-2.3655105952758646
2215	21	-2.0232556394697667
2215	22	-1.5910718573682345
2215	23	-2.203412577457069
2215	24	-1.93676346796137
2215	25	-1.9744018618892731
2216	1	-1.3764047871328202
2216	2	-1.4622003615540358
2216	3	-2.0024205934819967
2216	4	-2.522606624993184
2216	5	-1.5792983582114282
2216	6	-1.665358558636516
2216	7	-1.6274922687802196
2216	8	-2.0722519348732504
2216	9	-2.2175068246336274
2216	10	-1.6796854156078531
2216	11	-2.445470047529369
2216	12	-3.0256981228571433
2216	13	-3.289891515661857
2216	14	-2.8378887167094575
2216	15	-2.500444564144047
2216	16	-1.8265669000533222
2216	17	-1.8867718811810192
2216	18	-1.9675767714349597
2216	19	-2.2161291998010277
2216	20	-2.223432899138462
2216	21	-2.595166209327811
2216	22	-2.9887626891746635
2216	23	-3.1905779701637265
2216	24	-2.874844076007367
2216	25	-2.7841061674422605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82145.0
3	3.0
4	682.0
5	4010.0
6	5437.0
7	4603.0
8	4199.0
9	3803.0
10	3442.0
11	3330.0
12	3187.0
13	3187.0
14	3167.0
15	3140.0
16	3271.0
17	3418.0
18	3818.0
19	4651.0
20	5789.0
21	7733.0
22	9563.0
23	11684.0
24	13289.0
25	14908.0
26	16369.0
27	18460.0
28	20724.0
29	24382.0
30	30076.0
31	37770.0
32	46968.0
33	59619.0
34	77328.0
35	100129.0
36	136184.0
37	209733.0
38	556757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07637159508938	19.10510872077611	12.547517593425695	25.271002090708816
2	14.078089524880752	20.363564644129227	42.49763065258597	23.06071517840405
3	18.337971991012548	25.510556092176074	29.925655638898075	26.225816277913307
4	11.800493111025483	17.358211053601742	39.25615477541839	31.585141059954385
5	12.364950276185555	38.555841815911876	36.6702554081727	12.408952499729867
6	30.78046390132384	37.83458051635264	18.833016738217815	12.5519388441057
7	27.08646782901142	31.89343929447166	23.173120473961777	17.846972402555146
8	24.22583865580782	37.93647653273599	21.188286087288148	16.649398724168048
9	26.464562716955985	15.13885833807323	20.666965160789847	37.72961378418094
10	14.638425590595505	29.246785329165764	35.36935118281795	20.74543789742078
11	33.20949162425267	22.715029359530767	25.724584629180956	18.350894387035602
12	23.29872285040267	25.099747823964858	32.56396323110714	19.037566094525342
13	28.76546246837222	22.600128593592185	25.91427701246369	22.720131925571906
14	20.054565287001434	23.335901928268516	28.121949251159123	28.487583533570927
15	22.54802166490725	32.16784445627513	23.42625483731144	21.85787904150618
16	20.97311931172943	28.516502289137417	29.297900241115965	21.212478158017188
17	21.180265862112503	29.436556105548274	28.544969171917483	20.83820886042174
18	21.054030132452272	27.644209633709583	31.01774692413351	20.284013309704633
19	22.95442046929963	27.549079265494992	28.163574773273808	21.332925491931572
20	23.236057077182792	28.004550347328426	28.538591396771807	20.220801178716975
21	21.953014818483503	27.408955390956358	27.915292084031062	22.722737706529074
22	22.571017837189522	29.193015063571302	27.38847119751715	20.847495901722024
23	21.110390903517782	28.914534353251415	28.47108605135774	21.503988691873065
24	22.495343730924265	27.76711235827827	28.98861019966967	20.7489337111278
25	21.955484575921634	27.97445774444859	28.523465255230573	21.546592424399204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	223.0
1	223.0
2	407.0
3	591.0
4	591.0
5	591.0
6	1212.5
7	1834.0
8	1834.0
9	1834.0
10	2610.5
11	3387.0
12	3387.0
13	3387.0
14	5718.0
15	8049.0
16	8049.0
17	8049.0
18	14224.0
19	20399.0
20	20399.0
21	20399.0
22	32202.5
23	44006.0
24	44006.0
25	44006.0
26	62866.0
27	81726.0
28	81726.0
29	81726.0
30	106195.0
31	130664.0
32	130664.0
33	130664.0
34	145718.0
35	160772.0
36	160772.0
37	160772.0
38	170525.0
39	180278.0
40	180278.0
41	180278.0
42	186922.0
43	193566.0
44	193566.0
45	193566.0
46	201222.5
47	208879.0
48	208879.0
49	208879.0
50	195018.0
51	181157.0
52	181157.0
53	181157.0
54	155452.0
55	129747.0
56	129747.0
57	129747.0
58	109690.5
59	89634.0
60	89634.0
61	89634.0
62	71727.0
63	53820.0
64	53820.0
65	53820.0
66	40864.5
67	27909.0
68	27909.0
69	27909.0
70	20335.5
71	12762.0
72	12762.0
73	12762.0
74	8868.5
75	4975.0
76	4975.0
77	4975.0
78	3399.0
79	1823.0
80	1823.0
81	1823.0
82	1164.5
83	506.0
84	506.0
85	506.0
86	348.5
87	191.0
88	191.0
89	191.0
90	114.5
91	38.0
92	38.0
93	38.0
94	23.0
95	8.0
96	8.0
97	8.0
98	11.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3526446396062872
2	0.0435926030509617
3	0.038647770466076495
4	0.03838751611950359
5	0.043722730224248156
6	0.019193758059751796
7	0.019128694473108568
8	0.02550492596414476
9	0.023162636844988607
10	0.021536047178907947
11	0.02075528413918923
12	0.0216661743521944
13	0.021731237938837626
14	0.02927861398945189
15	0.03018950420245706
16	0.022967446085058928
17	0.02420365423128023
18	0.10000273267063901
19	0.02687126128365251
20	0.02420365423128023
21	0.02804240584323059
22	0.021926428698767306
23	0.023683145538134418
24	0.020820347725832456
25	0.023878336298064098
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1536958.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.95914031789611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.46545422478277	62.35486067118655
2	10.326562813038482	15.068342905560856
3	2.3393907340163533	5.120398104644554
4	0.7592442258214391	2.2157522402903513
5	0.3415927198346172	1.2461155588992807
6	0.1872787744640204	0.8198219030810451
7	0.11953967039528919	0.6105058110147469
8	0.07674424213968717	0.44793551446880253
9	0.05707230150836197	0.37475514486124695
>10	0.28327787544933297	3.6607993461605814
>50	0.02142966430806224	1.0591723521239014
>100	0.017858981548602052	2.739691938382992
>500	0.0026786898194006313	1.3395759934465354
>1k	0.0018750828735804416	2.9422725158786545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4437	0.28868713393599565	No Hit
CCCATGTACTCTGCGTTGATACCAC	3748	0.24385832273881264	No Hit
GTCCTACAGTGGACATTTCTAAATT	3586	0.23331802170260996	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3492	0.22720204455814666	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3359	0.21854858753459755	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3089	0.20098141914092643	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2960	0.19258821646395022	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2065	0.13435630641826257	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1866	0.12140865267626051	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1828	0.1189362363838179	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1668	0.10852606252090168	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1635	0.10637896416167521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.9519075992967928E-4	0.0
4	0.0	0.0	0.0	1.9519075992967928E-4	0.0
5	0.0	0.0	0.0	1.9519075992967928E-4	0.0
6	0.0	0.0	0.0	1.9519075992967928E-4	0.0
7	0.0	0.0	0.0	3.253179332161321E-4	0.0
8	0.0	0.0	0.0	3.253179332161321E-4	0.0
9	0.0	0.0	0.0	3.253179332161321E-4	0.0
10	0.0	6.506358664322643E-5	0.0	3.253179332161321E-4	6.506358664322643E-5
11	0.0	6.506358664322643E-5	0.0	3.253179332161321E-4	6.506358664322643E-5
12	0.0	6.506358664322643E-5	0.0	3.253179332161321E-4	1.9519075992967928E-4
13	0.0	6.506358664322643E-5	0.0	3.253179332161321E-4	1.9519075992967928E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	25	0.006048782	18.990889	9
TAGGACC	585	0.0	14.449352	4
GGCGAGG	840	0.0	12.321068	19
ATGTCGA	75	2.0698605E-4	11.402325	17
TGTCGAG	75	2.0698605E-4	11.402325	18
TGTAGGA	1975	0.0	11.397129	2
AGGACGT	1790	0.0	11.301254	5
TAGGACA	625	0.0	11.245168	4
GGACGTG	1805	0.0	11.204056	6
CTAGGAC	170	1.0913936E-11	11.173656	3
GTAGGAC	1960	0.0	11.096695	3
GTAATAC	120	1.2793862E-7	11.080542	3
TCGAACT	120	1.2840064E-7	11.077657	19
CTGTAGG	1970	0.0	10.980758	1
AATGTCC	505	0.0	10.905658	8
GGACCTG	1135	0.0	10.874778	6
TAGCACC	105	3.4759978E-6	10.854408	4
CGTGCGC	70	0.0014973651	10.851935	10
AGGACCT	1160	0.0	10.807271	5
AAATGTC	485	0.0	10.766979	7
>>END_MODULE