FastQCFastQC Report
Thu 26 May 2016
SRR1512938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536958
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC45970.29909730779891186No Hit
CCCATGTACTCTGCGTTGATACCAC43150.280749376365522No Hit
GTATCAACGCAGAGTACTTTTTTTT36840.23969425319364615No Hit
GTCCTACAGTGGACATTTCTAAATT33960.22095594024039694No Hit
CTGTAGGACGTGGAATATGGCAAGA33830.22011011361403499No Hit
CTTTAGGACGTGAAATATGGCGAGG32830.21360375494971234No Hit
GTCCTAAAGTGTGTATTTCTCATTT32280.21002525768433492No Hit
TATCAACGCAGAGTACTTTTTTTTT27900.18152740673460172No Hit
GGTATCAACGCAGAGTACTTTTTTT23480.15276930143829565No Hit
GTCCTACAGTGTGCATTTCTCATTT19110.12433651407520568No Hit
CTGAAGGACCTGGAATATGGCGAGA17100.11125873315991719No Hit
GAGTACATGGGAAGCAGTGGTATCA16880.1098273342537662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5750.014.0681734
TCCAACG951.0545991E-611.98590518
GTACTAG1052.6877024E-711.77261
CCAACGA855.38663E-511.16334219
GGCGAGG8950.011.1321619
GTCGAGG600.005927226511.07031519
GGACCTG11950.011.0653086
AGGACCT12350.010.9422855
TAGGACA6350.010.7905954
AGGACAT5650.010.6115475
CTAGGAC2350.010.5198533
GTAGGAC18300.010.4955333
CTAGCTC1002.3752345E-510.4591633
TGTAGGA18400.010.438152
GTCCTAC18950.010.3365681
CTAAGGT1303.748246E-710.2487594
GTCCTAT2250.010.1425481
TCCCGTT750.002666272210.12472511
CCCGTTT750.002667712310.12406612
TTAGGAC13600.010.0676423
Produced by FastQC (version 0.11.5)