##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1536958 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.467193638342753 31.0 31.0 34.0 27.0 34.0 2 30.636041453312323 31.0 31.0 34.0 27.0 34.0 3 30.728018592570518 31.0 31.0 34.0 27.0 34.0 4 34.190173056127755 37.0 35.0 37.0 30.0 37.0 5 33.86931588241188 37.0 35.0 37.0 30.0 37.0 6 33.72867833733908 37.0 35.0 37.0 29.0 37.0 7 33.59879254995907 37.0 35.0 37.0 28.0 37.0 8 33.478950628449184 37.0 35.0 37.0 28.0 37.0 9 34.92774363385337 39.0 35.0 39.0 28.0 39.0 10 34.81523698110163 39.0 35.0 39.0 27.0 39.0 11 34.84343553955281 39.0 35.0 39.0 27.0 39.0 12 34.70804016765585 39.0 35.0 39.0 27.0 39.0 13 34.69048666261537 39.0 35.0 39.0 27.0 39.0 14 35.73948735098812 40.0 36.0 41.0 27.0 41.0 15 35.75638501507523 40.0 36.0 41.0 27.0 41.0 16 35.73034071197782 40.0 36.0 41.0 27.0 41.0 17 35.717929182189756 40.0 36.0 41.0 27.0 41.0 18 35.65085903453445 40.0 36.0 41.0 27.0 41.0 19 35.62865022986965 40.0 36.0 41.0 27.0 41.0 20 35.613688858121044 40.0 36.0 41.0 27.0 41.0 21 35.55224671071038 40.0 36.0 41.0 27.0 41.0 22 35.531712642765775 40.0 36.0 41.0 27.0 41.0 23 35.419843613163145 40.0 36.0 41.0 26.0 41.0 24 35.414589728541706 40.0 36.0 41.0 26.0 41.0 25 35.31377565294562 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6362496915677553 1101 2 0.6531247340295003 1101 3 0.6816524187378228 1101 4 0.5206198195116158 1101 5 0.75262674066731 1101 6 0.8411093955102302 1101 7 0.7933070759343579 1101 8 0.7845464234499886 1101 9 0.6150178848036418 1101 10 0.9681833215637852 1101 11 0.9741689693606403 1101 12 1.0066247712923513 1101 13 0.9292149337240616 1101 14 0.9846604928712921 1101 15 0.9845364908851195 1101 16 1.1856591026138616 1101 17 1.2149953543208056 1101 18 0.7548440728614025 1101 19 0.9801599175197069 1101 20 0.9910477616830349 1101 21 1.0550821497877934 1101 22 0.9989477368291944 1101 23 1.1259438631152108 1101 24 0.982416307871766 1101 25 1.1647495123821159 1102 1 0.5500941047608023 1102 2 0.3875784293724891 1102 3 0.5053114546401112 1102 4 0.369658307864718 1102 5 0.48227551926751744 1102 6 0.3558533362313483 1102 7 0.45439069724802295 1102 8 0.48577812072706905 1102 9 0.6688137257272899 1102 10 0.7301357251740228 1102 11 0.6274833139770948 1102 12 0.8054858293474894 1102 13 0.8871681015481627 1102 14 0.8163096514998855 1102 15 0.8554958962671435 1102 16 0.7546203104459082 1102 17 0.5682431679884203 1102 18 0.7767131993313541 1102 19 0.8562843485813687 1102 20 0.7480203038529396 1102 21 0.8084421705594451 1102 22 0.6965463004119812 1102 23 0.8879286574763796 1102 24 0.7757846220613516 1102 25 0.9179853397397579 1103 1 0.682980924514009 1103 2 0.684616400627192 1103 3 0.7107596645628433 1103 4 0.6283995009634893 1103 5 0.5571801083771319 1103 6 0.692218630536729 1103 7 0.6937107789943013 1103 8 0.9187209600157047 1103 9 1.051135824310947 1103 10 0.9806867012750331 1103 11 0.8302041335318364 1103 12 0.980349585546179 1103 13 0.8884254400737817 1103 14 1.145299375552547 1103 15 0.9440996252707166 1103 16 1.0651953303595008 1103 17 1.0483847287845336 1103 18 1.1276472605498782 1103 19 1.176299045747335 1103 20 1.1866013032065794 1103 21 0.8523784497626821 1103 22 1.032328937909604 1103 23 1.133889115931403 1103 24 1.2731797639506937 1103 25 1.2468106258383287 1104 1 0.2925180377275929 1104 2 0.4307371099831947 1104 3 0.2634871658197575 1104 4 0.29070125150052917 1104 5 0.1840369117045242 1104 6 0.34611049031094154 1104 7 0.26602327042444074 1104 8 0.15938552420968222 1104 9 0.37359114676626604 1104 10 0.43934283272528063 1104 11 0.3961320781362545 1104 12 0.2553792943994182 1104 13 0.3323471229660484 1104 14 0.5168742875686405 1104 15 0.494050491398049 1104 16 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-2.0791644542066408 2216 20 -2.277520026076303 2216 21 -2.2215491024788676 2216 22 -2.5095885802713767 2216 23 -1.6249240572251793 2216 24 -1.9944396781923643 2216 25 -1.7811627700928128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38323.0 3 0.0 4 575.0 5 5365.0 6 6656.0 7 5238.0 8 4092.0 9 3648.0 10 3154.0 11 2767.0 12 2588.0 13 2400.0 14 2325.0 15 2378.0 16 2491.0 17 2635.0 18 2853.0 19 3442.0 20 4456.0 21 5804.0 22 7320.0 23 9040.0 24 10518.0 25 12200.0 26 13761.0 27 15263.0 28 17468.0 29 20901.0 30 26565.0 31 33361.0 32 43105.0 33 55210.0 34 72271.0 35 94296.0 36 129641.0 37 202186.0 38 672662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.21742267275969 19.837720560561447 13.613318869135913 27.33153789754295 2 12.978445706968838 21.02408917366535 41.02023740432387 24.97722771504194 3 16.744504404154178 25.525616184697302 30.61482486834383 27.115054542804685 4 11.015175862847741 18.009272005066215 38.406706876162175 32.568845255923875 5 11.930477769985655 38.82691004135469 36.75136204784798 12.491250140811687 6 30.87161181475262 38.3333517826248 18.367810718083323 12.427225684539254 7 26.746705633862405 32.069177620766304 23.243498325560505 17.940618419810782 8 24.29464206649755 38.19943274454752 20.82638805690265 16.67953713205228 9 25.962624476040254 15.00983960435872 20.547684812377003 38.47985110722402 10 14.274640795519536 29.578920764617983 35.45360272746577 20.692835712396707 11 33.05304157657444 22.563996119337528 25.79241579047025 18.59054651361778 12 23.014068028113932 25.285294196116865 32.507367836084924 19.19326993968428 13 28.685846653371026 22.382283184055645 26.061834472572055 22.87003569000127 14 20.012584525848382 23.124879620616237 28.12576457176173 28.73677128177365 15 22.484371980377702 32.150612009320525 23.440435235547618 21.92458077475415 16 20.941351486955824 28.515946567334545 29.154928569243875 21.38777337646576 17 20.970582492678165 29.408981451350474 28.693459160429548 20.926976895541817 18 20.932780709345376 27.65711720269892 31.099804152487785 20.31029793546792 19 22.87661267864068 27.437724642848778 28.268236249450183 21.417426429060356 20 23.116074240456417 27.92489022958739 28.715991993982477 20.243043535973722 21 21.796740297342136 27.279612219005173 28.122580435277662 22.801067048375028 22 22.480736160713413 28.955585325974155 27.569070371327054 20.994608141985378 23 20.837733261242832 28.814534173695165 28.665538463340223 21.68219410172178 24 22.429724728619426 27.82181142466017 29.0030423772704 20.745421469450005 25 21.843893920011865 27.881978920903283 28.51742298347689 21.75670417560795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 137.0 1 137.0 2 344.0 3 551.0 4 551.0 5 551.0 6 1158.5 7 1766.0 8 1766.0 9 1766.0 10 2550.0 11 3334.0 12 3334.0 13 3334.0 14 5635.5 15 7937.0 16 7937.0 17 7937.0 18 14318.5 19 20700.0 20 20700.0 21 20700.0 22 32961.5 23 45223.0 24 45223.0 25 45223.0 26 64793.5 27 84364.0 28 84364.0 29 84364.0 30 107470.0 31 130576.0 32 130576.0 33 130576.0 34 145424.5 35 160273.0 36 160273.0 37 160273.0 38 170111.5 39 179950.0 40 179950.0 41 179950.0 42 186543.5 43 193137.0 44 193137.0 45 193137.0 46 201073.5 47 209010.0 48 209010.0 49 209010.0 50 195383.5 51 181757.0 52 181757.0 53 181757.0 54 155688.0 55 129619.0 56 129619.0 57 129619.0 58 108959.5 59 88300.0 60 88300.0 61 88300.0 62 70854.0 63 53408.0 64 53408.0 65 53408.0 66 40575.0 67 27742.0 68 27742.0 69 27742.0 70 19941.5 71 12141.0 72 12141.0 73 12141.0 74 8405.0 75 4669.0 76 4669.0 77 4669.0 78 3161.5 79 1654.0 80 1654.0 81 1654.0 82 1079.0 83 504.0 84 504.0 85 504.0 86 332.5 87 161.0 88 161.0 89 161.0 90 101.5 91 42.0 92 42.0 93 42.0 94 22.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07885706701159043 2 1.3012717328645285E-4 3 0.0 4 0.032857111254829346 5 0.07931251211809301 6 0.07918238494480656 7 0.040534614478730066 8 0.07430261594656458 9 0.05328707746080244 10 0.11451191249207851 11 0.006961803770825227 12 0.013728416781720775 13 0.0073521852906845864 14 0.009759537996483963 15 0.008783584196835567 16 0.019193758059751796 17 0.0297991226825977 18 0.0031230521588748684 19 0.004814705411598756 20 0.007417248877327812 21 5.205086931458114E-4 22 7.156994530754907E-4 23 6.506358664322643E-5 24 1.3012717328645285E-4 25 0.005205086931458114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1536958.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.5700160974695 #Duplication Level Percentage of deduplicated Percentage of total 1 84.45071834029223 60.44139271057577 2 10.998452077091455 15.743187844093645 3 2.5319020770166385 5.436248172278917 4 0.83595399070734 2.3931696228667274 5 0.3821005737917175 1.3673472108562774 6 0.2011300682130595 0.8636929331816284 7 0.11567811201077863 0.5795359037115414 8 0.08017564376666063 0.45905376920039054 9 0.05603697970774707 0.3609510785763327 >10 0.30123675471249595 3.7987429832910937 >50 0.023445424779397855 1.1520337793352537 >100 0.01835444337476753 2.751006491967721 >500 0.002634904179854659 1.30178817720915 >1k 0.0021806103557417866 3.3518493228555686 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4597 0.29909730779891186 No Hit CCCATGTACTCTGCGTTGATACCAC 4315 0.280749376365522 No Hit GTATCAACGCAGAGTACTTTTTTTT 3684 0.23969425319364615 No Hit GTCCTACAGTGGACATTTCTAAATT 3396 0.22095594024039694 No Hit CTGTAGGACGTGGAATATGGCAAGA 3383 0.22011011361403499 No Hit CTTTAGGACGTGAAATATGGCGAGG 3283 0.21360375494971234 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3228 0.21002525768433492 No Hit TATCAACGCAGAGTACTTTTTTTTT 2790 0.18152740673460172 No Hit GGTATCAACGCAGAGTACTTTTTTT 2348 0.15276930143829565 No Hit GTCCTACAGTGTGCATTTCTCATTT 1911 0.12433651407520568 No Hit CTGAAGGACCTGGAATATGGCGAGA 1710 0.11125873315991719 No Hit GAGTACATGGGAAGCAGTGGTATCA 1688 0.1098273342537662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.506358664322643E-5 0.0 4 0.0 0.0 0.0 6.506358664322643E-5 0.0 5 0.0 0.0 0.0 6.506358664322643E-5 0.0 6 0.0 0.0 0.0 6.506358664322643E-5 0.0 7 0.0 0.0 0.0 6.506358664322643E-5 0.0 8 0.0 0.0 0.0 6.506358664322643E-5 0.0 9 0.0 0.0 0.0 6.506358664322643E-5 0.0 10 0.0 0.0 0.0 6.506358664322643E-5 0.0 11 0.0 0.0 0.0 6.506358664322643E-5 0.0 12 0.0 0.0 0.0 6.506358664322643E-5 1.9519075992967928E-4 13 0.0 0.0 0.0 6.506358664322643E-5 1.9519075992967928E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 575 0.0 14.068173 4 TCCAACG 95 1.0545991E-6 11.985905 18 GTACTAG 105 2.6877024E-7 11.7726 1 CCAACGA 85 5.38663E-5 11.163342 19 GGCGAGG 895 0.0 11.13216 19 GTCGAGG 60 0.0059272265 11.070315 19 GGACCTG 1195 0.0 11.065308 6 AGGACCT 1235 0.0 10.942285 5 TAGGACA 635 0.0 10.790595 4 AGGACAT 565 0.0 10.611547 5 CTAGGAC 235 0.0 10.519853 3 GTAGGAC 1830 0.0 10.495533 3 CTAGCTC 100 2.3752345E-5 10.459163 3 TGTAGGA 1840 0.0 10.43815 2 GTCCTAC 1895 0.0 10.336568 1 CTAAGGT 130 3.748246E-7 10.248759 4 GTCCTAT 225 0.0 10.142548 1 TCCCGTT 75 0.0026662722 10.124725 11 CCCGTTT 75 0.0026677123 10.124066 12 TTAGGAC 1360 0.0 10.067642 3 >>END_MODULE