FastQCFastQC Report
Thu 26 May 2016
SRR1512937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292226
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42480.18532204067138233No Hit
GTCCTAAAGTGTGTATTTCTCATTT41410.18065408908196662No Hit
CTGTAGGACGTGGAATATGGCAAGA40280.1757243831978173No Hit
CTTTAGGACGTGAAATATGGCGAGG35100.15312626241915064No Hit
GTACATGGGAAGCAGTGGTATCAAC33530.14627702504028836No Hit
GTATCAACGCAGAGTACTTTTTTTT28560.12459504429318924No Hit
CCCATGTACTCTGCGTTGATACCAC26710.11652428687223684No Hit
GTCCTACAGTGTGCATTTCTCATTT23670.10326206927240159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG400.005291674314.2453035
GGCGAGG9550.012.42665919
TAGGACC7550.011.6991514
AATCCCG2350.011.31196219
GTCCTAA11500.011.267471
TAGAACG855.3383475E-511.1737614
CGTGCGC855.352605E-511.170835510
TAGGACT3150.011.1560254
CGTACTG600.005891539611.079685
TACCGAA600.005904949711.0762967
AGGACCT14850.010.6799795
CACCTTT6700.010.49640814
GGCGTGC1002.4148705E-510.4440488
GTCCTAG3650.010.441251
TGGCGAG21800.010.37402718
CTCGAAC1409.660107E-810.18099218
AGGACGT23800.010.1752165
TGTAGGA24550.010.1749452
TCGAACT1409.784162E-810.17210819
GTAGGAC23600.010.1416093
Produced by FastQC (version 0.11.5)