##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512937_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2292226
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.884632230853327	31.0	30.0	34.0	23.0	34.0
2	28.99211421561399	31.0	30.0	34.0	20.0	34.0
3	29.217698865644138	31.0	30.0	34.0	23.0	34.0
4	32.620872898222075	35.0	33.0	37.0	25.0	37.0
5	32.74793454048597	35.0	33.0	37.0	26.0	37.0
6	32.77787705051771	37.0	35.0	37.0	25.0	37.0
7	32.59732024678195	36.0	33.0	37.0	25.0	37.0
8	32.5902616059673	36.0	33.0	37.0	25.0	37.0
9	33.89282121396407	38.0	34.0	39.0	23.0	39.0
10	33.76082244944434	38.0	34.0	39.0	22.0	39.0
11	33.820496757300546	38.0	34.0	39.0	23.0	39.0
12	33.710397229592544	38.0	34.0	39.0	22.0	39.0
13	33.70388391022526	38.0	34.0	39.0	22.0	39.0
14	34.68084604223144	39.0	34.0	41.0	20.0	41.0
15	34.62044056737861	39.0	34.0	41.0	19.0	41.0
16	34.5014296147064	39.0	34.0	41.0	18.0	41.0
17	34.44620076728909	39.0	34.0	41.0	18.0	41.0
18	34.14005774299742	39.0	33.0	41.0	17.0	41.0
19	34.379169854979395	39.0	34.0	41.0	18.0	41.0
20	34.45065233532819	39.0	34.0	41.0	18.0	41.0
21	34.376735540038375	39.0	34.0	41.0	18.0	41.0
22	34.29346408251193	39.0	34.0	41.0	17.0	41.0
23	34.02162526731657	39.0	34.0	41.0	10.0	41.0
24	34.00884816767631	39.0	34.0	41.0	2.0	41.0
25	33.92276067019569	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5790354329096381
1101	2	0.8500382934823847
1101	3	0.7923326228983107
1101	4	0.8307606289989096
1101	5	0.706711348331666
1101	6	0.837004159988318
1101	7	0.8856897915508029
1101	8	0.599841352374682
1101	9	0.47906242599849946
1101	10	0.6812545192815023
1101	11	0.8121970948677628
1101	12	1.0212491445728276
1101	13	0.8184574008602539
1101	14	0.7354596950283323
1101	15	0.6301326478212985
1101	16	1.1141932826770287
1101	17	1.0570185317569312
1101	18	1.286385641900523
1101	19	0.6580350846651513
1101	20	0.8008000851820967
1101	21	0.8105809440652862
1101	22	1.0209955173331764
1101	23	1.1551974363969606
1101	24	0.5778020061268876
1101	25	0.8952328129234814
1102	1	0.5436337282567472
1102	2	0.6448449050528922
1102	3	0.6966040628406525
1102	4	0.8701162053601408
1102	5	0.6928359787670999
1102	6	0.666486499406318
1102	7	0.879479035778715
1102	8	0.9108981719424278
1102	9	0.9253018558309591
1102	10	1.0374283096699628
1102	11	0.9687914582438708
1102	12	1.0285953252119455
1102	13	0.9738793620831458
1102	14	0.9702304687843935
1102	15	1.1327107026271932
1102	16	1.0560582440083905
1102	17	1.0292669991237986
1102	18	1.383378095122076
1102	19	1.3133528984886382
1102	20	1.2827969475085936
1102	21	0.9795875349888163
1102	22	1.2693823435481022
1102	23	1.5086920398953154
1102	24	1.4096162950866002
1102	25	1.2538694016624987
1103	1	0.45974388032776403
1103	2	0.5608042488155256
1103	3	0.5263861175711355
1103	4	0.6764744425512319
1103	5	0.23015437491139323
1103	6	0.567094554455096
1103	7	0.4347353635543385
1103	8	0.5673844209277235
1103	9	0.7518988640153523
1103	10	0.7309899555650432
1103	11	0.751760464098524
1103	12	0.9125097729672689
1103	13	0.8524324809991555
1103	14	1.1324389816797122
1103	15	1.0197999145439454
1103	16	1.1350208043657943
1103	17	0.9999294082600443
1103	18	1.2378208915287559
1103	19	1.2900642319900726
1103	20	1.0825462598546451
1103	21	1.08028409644254
1103	22	1.1557993056191123
1103	23	1.4626024190979763
1103	24	1.2176297625178094
1103	25	1.1536954501989385
1104	1	0.5973323845612164
1104	2	0.5797991124635544
1104	3	0.7228349160503207
1104	4	0.9385038764144582
1104	5	0.7566092004461353
1104	6	0.7570276297514837
1104	7	0.9480630530553
1104	8	0.8766376350670342
1104	9	1.003551775292216
1104	10	0.9712906347991108
1104	11	1.0083927219682423
1104	12	0.9409827911517894
1104	13	0.9840285358906513
1104	14	1.1306043877743548
1104	15	1.2994053572422075
1104	16	1.1256889714092964
1104	17	1.1494101408434219
1104	18	1.3092376676462862
1104	19	1.2649986485676123
1104	20	1.203514722909027
1104	21	1.2549558309455193
1104	22	1.1538365061389015
1104	23	1.493466517704782
1104	24	1.3378728885970759
1104	25	1.0832111838332779
1105	1	0.704776451676814
1105	2	0.7622096642780178
1105	3	0.8872835845173483
1105	4	0.8362831738101164
1105	5	0.7285633646498582
1105	6	0.5149343432429845
1105	7	0.8261179679245032
1105	8	0.9399496808719192
1105	9	0.7205010522502988
1105	10	0.9034838683785438
1105	11	0.878381170296727
1105	12	0.8525908688222898
1105	13	0.917030772819686
1105	14	1.0164808273869284
1105	15	1.1277146793252228
1105	16	0.9944418931322332
1105	17	0.9198552472231114
1105	18	1.0295844495978628
1105	19	1.0496982857910382
1105	20	1.0823353706525864
1105	21	0.9951904187362928
1105	22	0.7820882630361012
1105	23	1.04948246596566
1105	24	1.1626205096867963
1105	25	1.233886319150102
1106	1	0.4926271759149259
1106	2	0.5465975158470258
1106	3	0.5641048162405227
1106	4	0.5549899512596284
1106	5	0.5761889034797889
1106	6	0.5187681474725281
1106	7	0.2985939245708238
1106	8	0.5491992394787246
1106	9	0.6167495748598455
1106	10	0.7709444924190549
1106	11	0.7167651374520077
1106	12	0.788178948940434
1106	13	0.7182013959616711
1106	14	0.9823959800563742
1106	15	0.9065395558541596
1106	16	0.829069698254159
1106	17	0.8150796761766586
1106	18	1.0890651300537613
1106	19	0.6924213842292133
1106	20	0.7279165620417558
1106	21	0.6403556752742503
1106	22	0.8143050898125992
1106	23	1.1657014676452206
1106	24	0.9426353623086996
1106	25	1.0194309703246773
1107	1	0.44631740782082474
1107	2	0.6103717319207078
1107	3	0.6734072916920972
1107	4	0.8085910442207691
1107	5	0.7098388589103166
1107	6	0.6176152933792167
1107	7	0.8026945356394535
1107	8	0.7685282975857888
1107	9	0.9736933063080073
1107	10	0.6915696357808514
1107	11	0.7992043109701896
1107	12	1.0060119328035668
1107	13	0.8929986940315899
1107	14	1.1156473811871521
1107	15	1.2616227361735923
1107	16	1.0735070714246504
1107	17	1.1841910136796088
1107	18	1.5230846285447797
1107	19	1.371976987476316
1107	20	1.2190758582810375
1107	21	1.230129406539902
1107	22	1.2629668205045448
1107	23	1.263786491098429
1107	24	1.1640598027973041
1107	25	1.2631645312850495
1108	1	0.389521004322674
1108	2	0.5546847478088743
1108	3	0.4400636627650556
1108	4	0.5422402803583637
1108	5	0.5568067892721871
1108	6	0.5960549418093919
1108	7	0.611813487243694
1108	8	0.648685437789986
1108	9	0.7154006724347823
1108	10	0.7211981214556218
1108	11	0.5471392021098467
1108	12	0.6384805795342317
1108	13	0.5898011414977589
1108	14	0.7334954202989437
1108	15	0.7399573043359311
1108	16	0.6989825448156992
1108	17	0.8533275606708628
1108	18	0.879868597297552
1108	19	0.9129675550031351
1108	20	0.7945380150824377
1108	21	0.6568433301793064
1108	22	0.6613012167442989
1108	23	0.7499393868897855
1108	24	0.8354337147015087
1108	25	0.9209069091492452
1109	1	0.0331705286300128
1109	2	0.0563721854887298
1109	3	0.3092005980486974
1109	4	0.4075946848831933
1109	5	0.17117188374312775
1109	6	0.1538851923967286
1109	7	0.30511174611481096
1109	8	0.3591009622166439
1109	9	0.5498755355790408
1109	10	0.6915173994368331
1109	11	0.2148403949635238
1109	12	0.5220445388198272
1109	13	0.5509403607879548
1109	14	0.3559624882765462
1109	15	0.6289358363787798
1109	16	0.591433959962302
1109	17	0.429415659689937
1109	18	-1.236732210058996
1109	19	0.29956123194120465
1109	20	0.5184871305980607
1109	21	0.6443949262851305
1109	22	0.6687498052907443
1109	23	0.20702477862247548
1109	24	0.7007156787198809
1109	25	0.8200228508900338
1110	1	0.5282862104046906
1110	2	0.40937025357770196
1110	3	0.5032357288395843
1110	4	0.35384314444783627
1110	5	0.5530500534489988
1110	6	0.46940046032354843
1110	7	0.5432385616949915
1110	8	0.41698865234640436
1110	9	0.7291734967202572
1110	10	0.8627743706486868
1110	11	0.7353462527497499
1110	12	0.22548800720863227
1110	13	0.5022996400460045
1110	14	0.6414130857145537
1110	15	0.7373259916558652
1110	16	0.8199467460640832
1110	17	0.5036137092681372
1110	18	0.9506390447035429
1110	19	0.8435433846772682
1110	20	0.9151726365775446
1110	21	0.8764660767443075
1110	22	0.9753858400441118
1110	23	0.8180473091704954
1110	24	1.0154206498524019
1110	25	0.9920965796345769
1111	1	0.1769532850549531
1111	2	0.48202062850849003
1111	3	0.45392025169050143
1111	4	0.3544870223165688
1111	5	0.29548249458662923
1111	6	0.45057140391598693
1111	7	0.3184895252878235
1111	8	0.24983938403277506
1111	9	0.5118993792915347
1111	10	0.4287699349774243
1111	11	0.2107584158757021
1111	12	0.47721733616764794
1111	13	0.4710142769942891
1111	14	0.4453676124152395
1111	15	0.6325597923298076
1111	16	0.5231467331803898
1111	17	0.5608396202142174
1111	18	0.7724887901739308
1111	19	0.4448808346570985
1111	20	0.3882542317627298
1111	21	0.5052803782564581
1111	22	0.24559835961888865
1111	23	0.6125491934105227
1111	24	0.7804589098177033
1111	25	0.21375276296338797
1112	1	-0.21636238705853117
1112	2	0.09427887717050609
1112	3	0.1950985948109647
1112	4	0.2260389905324942
1112	5	0.2055826768614324
1112	6	0.23685281418122628
1112	7	-0.06394108096339579
1112	8	0.17270553677936817
1112	9	0.45855729187002225
1112	10	0.3970991131797419
1112	11	0.3595777617983913
1112	12	0.3301054200477296
1112	13	-0.002200355800788145
1112	14	0.30259861168215707
1112	15	0.2836261994346998
1112	16	0.38044438289136906
1112	17	0.41451266420350663
1112	18	0.3832215777541421
1112	19	0.26279710149762536
1112	20	0.11349699701389682
1112	21	0.2097051719155587
1112	22	0.24630641536957398
1112	23	0.4536057904270905
1112	24	0.18375799201584186
1112	25	0.39054838408277703
1113	1	-0.4288627449990834
1113	2	-0.24765010026492007
1113	3	-0.4119834976617831
1113	4	-0.054183123512139275
1113	5	-0.4555230850269609
1113	6	-0.1439183806720692
1113	7	-0.29517127491944706
1113	8	-0.026278389303520555
1113	9	-0.19134105301285587
1113	10	-0.26440336615515747
1113	11	-0.1805494622055832
1113	12	-0.008457723052131882
1113	13	-0.07449450418862824
1113	14	-0.15211624094178688
1113	15	-0.5178820427431106
1113	16	-0.4507422019739522
1113	17	-0.49533404178889384
1113	18	-0.5520839483707434
1113	19	-0.4975864556678715
1113	20	-0.5128977780085364
1113	21	-0.3455644289091353
1113	22	-0.30525014878291756
1113	23	-0.8407472200915862
1113	24	-0.2092285207841158
1113	25	-0.17313699571601404
1114	1	-1.2867023716569008
1114	2	-1.0818282008667133
1114	3	-0.8943528988518992
1114	4	-1.1148993334227093
1114	5	-1.08719906901284
1114	6	-1.515013164175933
1114	7	-1.6134367983729945
1114	8	-1.1662414206823506
1114	9	-1.286027745752385
1114	10	-1.40192033816772
1114	11	-2.0811184981591886
1114	12	-1.6353580923775581
1114	13	-1.5980437353519434
1114	14	-1.8621473016790375
1114	15	-1.757230864653934
1114	16	-2.472114385431638
1114	17	-4.621480742513832
1114	18	-3.449643421068803
1114	19	-2.2664686088675694
1114	20	-1.53539794637571
1114	21	-2.5155590531857612
1114	22	-2.6674707795344617
1114	23	-2.4233693524378097
1114	24	-2.003086666628576
1114	25	-1.9149121088828096
1115	1	-1.177888288509024
1115	2	-0.6150120006554296
1115	3	-0.7232358290717045
1115	4	-0.734291825499664
1115	5	-0.6141618694881146
1115	6	-0.7255286563612913
1115	7	-1.8261007060554277
1115	8	-1.3355326295553098
1115	9	-1.0788286673976586
1115	10	-0.7272711096417837
1115	11	-1.5356186395093019
1115	12	-1.029499608288475
1115	13	-0.8432723718228274
1115	14	-0.9645187952927756
1115	15	-2.331997636961212
1115	16	-1.0811399623632951
1115	17	-1.060963231041093
1115	18	-1.0884566752460714
1115	19	-1.1803316544205629
1115	20	-0.9290362346151682
1115	21	-1.7586137673715712
1115	22	-1.7353916362224453
1115	23	-1.1150066331591404
1115	24	-0.9916824754381892
1115	25	-1.2609382575916328
1116	1	-1.1694602538444379
1116	2	-2.073881265167106
1116	3	-1.715878063562542
1116	4	-1.3438501205889253
1116	5	-1.826591649142589
1116	6	-1.6137148227975224
1116	7	-1.2438990525442577
1116	8	-1.9446917553107426
1116	9	-1.9317505550572704
1116	10	-2.900613289587202
1116	11	-1.5520710495738186
1116	12	-1.4119397495446009
1116	13	-2.018986249098244
1116	14	-1.7237684148797712
1116	15	-1.4908347332910878
1116	16	-1.2947752160255632
1116	17	-1.6784420537754343
1116	18	-1.472854426341108
1116	19	-1.9106619916665153
1116	20	-2.2279984957327486
1116	21	-2.804152166563501
1116	22	-2.1330654609975355
1116	23	-1.9037839931319596
1116	24	-2.4739119810041146
1116	25	-2.2988441364753136
1201	1	0.30509632270481646
1201	2	0.22909757507238382
1201	3	0.5539455064304342
1201	4	0.49561733027822896
1201	5	0.5528759618106847
1201	6	0.5485145636980988
1201	7	0.38772624746619044
1201	8	0.04360836569020421
1201	9	0.2675747121506262
1201	10	0.5680189678264469
1201	11	0.0627962541349234
1201	12	-0.3584112551213323
1201	13	-0.0547671582545064
1201	14	0.45731700224123273
1201	15	0.1329412119872515
1201	16	0.6096616765155716
1201	17	0.6823532480291874
1201	18	1.005511595772525
1201	19	0.4759033998890345
1201	20	0.5261631392962727
1201	21	0.40687418784058593
1201	22	0.802593435350623
1201	23	1.0032433495773887
1201	24	0.8046510829596585
1201	25	0.6202503771619092
1202	1	0.5717457610393808
1202	2	0.6809026245002521
1202	3	0.405086665351984
1202	4	0.653471814115214
1202	5	0.7911531176965596
1202	6	0.7475389276128084
1202	7	0.7951134400549549
1202	8	0.7220131966017576
1202	9	1.1256449317791493
1202	10	1.1619765665022683
1202	11	0.7417501563521469
1202	12	0.8838747591803653
1202	13	0.9486450328622169
1202	14	1.1444767154222149
1202	15	0.9076680665953916
1202	16	1.1134408965301006
1202	17	1.2344880346486633
1202	18	1.5339985338617481
1202	19	1.3374392947918494
1202	20	1.1766610162859834
1202	21	1.3214308745052037
1202	22	1.2496508253237337
1202	23	1.2835858771699762
1202	24	1.4806645014314768
1202	25	1.2421738114009315
1203	1	0.21814469077608223
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2211	13	0.2532963007519413
2211	14	0.48593416819398527
2211	15	0.4216963190934706
2211	16	0.2909800257721571
2211	17	0.40775123226946874
2211	18	0.47227799684704763
2211	19	0.23751263362757413
2211	20	0.028563584255223873
2211	21	0.19773822844793898
2211	22	0.17238824295629485
2211	23	0.6082539693850393
2211	24	0.5038838939639234
2211	25	0.4607734915643178
2212	1	-0.08533062762312937
2212	2	-0.027341454756257377
2212	3	-0.14117309124298671
2212	4	-0.15851135568392039
2212	5	-0.07814353288038944
2212	6	-0.2529634023618499
2212	7	-0.36581986963807367
2212	8	-0.2866881317342518
2212	9	-0.17742056909185777
2212	10	-0.03133396930842025
2212	11	-0.24395061304436894
2212	12	-0.14934461304233082
2212	13	-0.26075437353291164
2212	14	-0.19620231257962217
2212	15	-0.30203529793413253
2212	16	-0.048628817456666695
2212	17	-0.17084526582146964
2212	18	-2.9110367238506996
2212	19	-1.0593667198105479
2212	20	-0.4107197519020147
2212	21	-0.4096911627378148
2212	22	-0.14796259049624183
2212	23	-0.2897923833435314
2212	24	-0.6695304913293398
2212	25	-0.23813172098694935
2213	1	-0.04935475774287923
2213	2	0.033906043692223164
2213	3	-0.39081705110166354
2213	4	-0.3749299686516352
2213	5	-0.24922876837695895
2213	6	-0.11905411304240232
2213	7	-0.0438069563046497
2213	8	-0.5479302827172319
2213	9	-0.31921362997553615
2213	10	-0.33626387891636256
2213	11	-0.37289654722390964
2213	12	-0.45302343853519744
2213	13	-0.20448304186042066
2213	14	-0.29685020736123846
2213	15	-0.3827880746573129
2213	16	-0.9654194691957372
2213	17	-0.5707236428115792
2213	18	-0.311178654497418
2213	19	-0.2709629056352867
2213	20	-1.0076615427507036
2213	21	-0.5526576421662668
2213	22	-0.45275666493152755
2213	23	-1.0175470263480833
2213	24	-0.6774110003030174
2213	25	-0.7140256727886225
2214	1	-1.7727545908869047
2214	2	-3.7504838819727837
2214	3	-1.9139161076775828
2214	4	-1.4763750252377044
2214	5	-1.513496962868384
2214	6	-1.599611965249526
2214	7	-1.7456649693711839
2214	8	-1.4614197591552518
2214	9	-1.6381131423738395
2214	10	-1.6848582990469367
2214	11	-1.5823360610178057
2214	12	-2.1434092091853287
2214	13	-1.9808561957635575
2214	14	-3.161944215009399
2214	15	-3.3270142482568943
2214	16	-2.45652113531861
2214	17	-2.8390205167030764
2214	18	-2.2280756955775693
2214	19	-4.577685558018203
2214	20	-2.7610319095385947
2214	21	-2.528000434220157
2214	22	-2.882092344106958
2214	23	-2.887000397954111
2214	24	-3.0833726207671326
2214	25	-2.791989214816123
2215	1	-0.7546983469349584
2215	2	-2.488622952654513
2215	3	-1.2987083917936637
2215	4	-0.7917058095375467
2215	5	-0.6556232816391798
2215	6	-1.3408406432250004
2215	7	-1.7704624122455215
2215	8	-1.6480569736955317
2215	9	-1.4643749308107985
2215	10	-2.9302363213880653
2215	11	-1.936022465835343
2215	12	-2.049075161043664
2215	13	-1.433839256154748
2215	14	-2.7409641689514643
2215	15	-2.7558319822470416
2215	16	-2.3221937294996664
2215	17	-2.6644659232166
2215	18	-1.4578594323392835
2215	19	-1.8924197133811163
2215	20	-2.2700136279782868
2215	21	-1.9929857840210587
2215	22	-1.5791385335379076
2215	23	-2.25547423158584
2215	24	-2.047938442139227
2215	25	-2.0402762879120218
2216	1	-1.9658693239464533
2216	2	-1.869435387346261
2216	3	-2.466743266401817
2216	4	-2.994608761470669
2216	5	-2.2343458521720976
2216	6	-2.1853906693483047
2216	7	-2.1191595125590013
2216	8	-2.7328074961615734
2216	9	-2.9573249046875
2216	10	-2.494267799966959
2216	11	-3.004991248489471
2216	12	-3.490026702318481
2216	13	-4.266584815193408
2216	14	-3.5557695503516733
2216	15	-3.286308079425723
2216	16	-2.443036859071242
2216	17	-2.6968392252019697
2216	18	-2.607232473091635
2216	19	-2.935794190915079
2216	20	-2.7448405611026345
2216	21	-3.1243174193397607
2216	22	-3.590018888814818
2216	23	-3.609307826152193
2216	24	-3.6750478987745474
2216	25	-3.547424276417768
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113848.0
3	5.0
4	882.0
5	5524.0
6	6699.0
7	6287.0
8	5502.0
9	5255.0
10	4672.0
11	4604.0
12	4496.0
13	4369.0
14	4483.0
15	4522.0
16	4654.0
17	5088.0
18	5591.0
19	6829.0
20	8493.0
21	11180.0
22	13757.0
23	16731.0
24	18947.0
25	22036.0
26	24347.0
27	27413.0
28	31172.0
29	36774.0
30	45196.0
31	56334.0
32	70864.0
33	89967.0
34	115833.0
35	151506.0
36	206012.0
37	313638.0
38	838716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51235474056993	18.56720121640749	12.27221364360539	25.648230399417187
2	14.65136831906569	20.245579503472896	41.416653755594496	23.68639842186692
3	18.633175250380443	24.948667045181292	29.565119862722263	26.853037841716006
4	12.12235473201353	16.899820764832725	38.701703716020276	32.27612078713347
5	12.976244355246635	38.010253626018915	36.16386773023196	12.849634288502488
6	31.55715816627513	37.246183812313035	18.482853771856032	12.71380424955581
7	27.68684075262521	31.488495159737816	22.778510335462936	18.04615375217404
8	24.844077338861737	37.010343062581576	21.02482849925448	17.120751099302215
9	26.5707108413705	15.02961275427632	20.140600039533123	38.259076364820054
10	14.723760944075853	28.96771702159689	35.03919468182819	21.269327352499058
11	34.141656641712274	22.239515904879216	25.120026739029598	18.498800714378905
12	23.25767704235333	24.69372990634305	32.14378593295656	19.90480711834706
13	29.034695965773103	22.017178899232686	25.723928936867434	23.22419619812677
14	20.66444898570115	22.203559722689512	27.88343254422816	29.248558747381175
15	23.001046442314323	31.046564501080915	23.238743326639245	22.713645729965517
16	22.089382778754473	27.93127838728581	28.06052521687679	21.91881361708292
17	21.741405183311436	28.571397403064363	27.923363475427205	21.763833938197
18	21.666131670258785	27.444922665723325	29.862713370761245	21.026232293256644
19	23.552616191289076	27.017976551413565	27.332727656729734	22.096679600567626
20	23.6464830923566	27.497005517701805	27.81406064890268	21.042450741038916
21	22.884748897182835	26.887313009817028	27.088174317767567	23.139763775232574
22	23.055854450125622	28.277128063887513	26.997466595804653	21.669550890182208
23	22.23998177791043	27.901309232699923	27.570815682865824	22.287893306523824
24	23.091184618559815	27.091139239401674	28.148997229689666	21.668678912348845
25	22.800530430597416	27.342178788623233	27.7266929729291	22.13059780785025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	245.0
1	245.0
2	416.0
3	587.0
4	587.0
5	587.0
6	1121.0
7	1655.0
8	1655.0
9	1655.0
10	2544.5
11	3434.0
12	3434.0
13	3434.0
14	6331.0
15	9228.0
16	9228.0
17	9228.0
18	16997.0
19	24766.0
20	24766.0
21	24766.0
22	41009.5
23	57253.0
24	57253.0
25	57253.0
26	82610.5
27	107968.0
28	107968.0
29	107968.0
30	142054.5
31	176141.0
32	176141.0
33	176141.0
34	200156.0
35	224171.0
36	224171.0
37	224171.0
38	241460.5
39	258750.0
40	258750.0
41	258750.0
42	271838.0
43	284926.0
44	284926.0
45	284926.0
46	294717.0
47	304508.0
48	304508.0
49	304508.0
50	290864.0
51	277220.0
52	277220.0
53	277220.0
54	246105.0
55	214990.0
56	214990.0
57	214990.0
58	184400.0
59	153810.0
60	153810.0
61	153810.0
62	125803.5
63	97797.0
64	97797.0
65	97797.0
66	75699.5
67	53602.0
68	53602.0
69	53602.0
70	39538.5
71	25475.0
72	25475.0
73	25475.0
74	17864.5
75	10254.0
76	10254.0
77	10254.0
78	7011.5
79	3769.0
80	3769.0
81	3769.0
82	2472.5
83	1176.0
84	1176.0
85	1176.0
86	764.0
87	352.0
88	352.0
89	352.0
90	231.0
91	110.0
92	110.0
93	110.0
94	65.5
95	21.0
96	21.0
97	21.0
98	19.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35437168935349306
2	0.039743027083716874
3	0.035467706936401555
4	0.03590396409429088
5	0.040964547125806966
6	0.01788654347346204
7	0.017799292041884178
8	0.023776015104967834
9	0.02080946643132047
10	0.020111454978697565
11	0.019413443526074655
12	0.019980577831330767
13	0.020111454978697565
14	0.028051335252283153
15	0.028443966694383538
16	0.02124572358920979
17	0.0229035007891892
18	0.10867165803022914
19	0.025041160862846856
20	0.022292740768144154
21	0.025957300894414424
22	0.01954432067344145
23	0.021900109326043766
24	0.018540929210296018
25	0.0219437350418327
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2292226.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.58387796252318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53911927582801	59.8005411933754
2	11.213374023100789	16.053935952355488
3	2.831021693438414	6.079665342370535
4	1.0064439199501645	2.8818063496734414
5	0.45611322027644396	1.6325176548681208
6	0.2467258469392003	1.0596955750497532
7	0.1490615868047862	0.7469284507113709
8	0.10602443567016812	0.6071712211246962
9	0.07419743178062893	0.47802059115454626
>10	0.34000401524424406	4.208651960244037
>50	0.019635739414664736	0.9875220568124156
>100	0.014491975401135905	2.139534672970022
>500	0.0025041980999248063	1.324252018582647
>1k	0.0012826380511804189	1.9997569607076553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4248	0.18532204067138233	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4141	0.18065408908196662	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4028	0.1757243831978173	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3510	0.15312626241915064	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3353	0.14627702504028836	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2856	0.12459504429318924	No Hit
CCCATGTACTCTGCGTTGATACCAC	2671	0.11652428687223684	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2367	0.10326206927240159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.3625715788931806E-5	0.0	0.0	0.0	0.0
6	4.3625715788931806E-5	0.0	0.0	0.0	0.0
7	4.3625715788931806E-5	0.0	0.0	4.3625715788931806E-5	0.0
8	4.3625715788931806E-5	0.0	0.0	4.3625715788931806E-5	4.3625715788931806E-5
9	4.3625715788931806E-5	0.0	0.0	4.3625715788931806E-5	4.3625715788931806E-5
10	4.3625715788931806E-5	0.0	0.0	8.725143157786361E-5	4.3625715788931806E-5
11	4.3625715788931806E-5	0.0	0.0	8.725143157786361E-5	4.3625715788931806E-5
12	4.3625715788931806E-5	0.0	0.0	8.725143157786361E-5	4.3625715788931806E-5
13	4.3625715788931806E-5	0.0	0.0	8.725143157786361E-5	4.3625715788931806E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCCG	40	0.0052916743	14.245303	5
GGCGAGG	955	0.0	12.426659	19
TAGGACC	755	0.0	11.699151	4
AATCCCG	235	0.0	11.311962	19
GTCCTAA	1150	0.0	11.26747	1
TAGAACG	85	5.3383475E-5	11.173761	4
CGTGCGC	85	5.352605E-5	11.1708355	10
TAGGACT	315	0.0	11.156025	4
CGTACTG	60	0.0058915396	11.07968	5
TACCGAA	60	0.0059049497	11.076296	7
AGGACCT	1485	0.0	10.679979	5
CACCTTT	670	0.0	10.496408	14
GGCGTGC	100	2.4148705E-5	10.444048	8
GTCCTAG	365	0.0	10.44125	1
TGGCGAG	2180	0.0	10.374027	18
CTCGAAC	140	9.660107E-8	10.180992	18
AGGACGT	2380	0.0	10.175216	5
TGTAGGA	2455	0.0	10.174945	2
TCGAACT	140	9.784162E-8	10.172108	19
GTAGGAC	2360	0.0	10.141609	3
>>END_MODULE