FastQCFastQC Report
Thu 26 May 2016
SRR1512937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292226
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA40580.17703315467148525No Hit
GTCCTACAGTGGACATTTCTAAATT38990.1700966658610451No Hit
GTCCTAAAGTGTGTATTTCTCATTT38790.1692241515452665No Hit
CTTTAGGACGTGAAATATGGCGAGG36840.16071713696642476No Hit
GTATCAACGCAGAGTACTTTTTTTT34110.1488073165560464No Hit
CCCATGTACTCTGCGTTGATACCAC31960.13942778766142605No Hit
GTACATGGGAAGCAGTGGTATCAAC31370.1368538704298791No Hit
TATCAACGCAGAGTACTTTTTTTTT25570.11155095527229864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG604.0336655E-412.6887364
TAGGACC6850.012.3645724
TCCAACG2450.012.00536518
CGTGCGC1459.640644E-1111.79403910
CGGTAGG657.963487E-411.7032051
CCAACGT1001.960003E-611.38573419
GGCGAGG9850.010.69218519
GTCTTAG3150.010.5653931
GTAGGAC22550.010.4997993
GACGTGG11950.010.4982257
GTAATAC1451.4235411E-810.4925293
TGTAGGA23300.010.2429962
ATATCGA750.002616582710.1481036
CGTGGAA12700.010.1009919
GTGCGCC1606.2664185E-910.08485811
GGACGTG22750.010.0365846
ACGTGGA12950.09.9804598
CTGTAGG23000.09.9636261
TGGCAAG17350.09.8982618
GTCCTAC22100.09.8960931
Produced by FastQC (version 0.11.5)