##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512937_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2292226 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.416609008012298 31.0 31.0 34.0 27.0 34.0 2 30.539202068207935 31.0 31.0 34.0 27.0 34.0 3 30.64803165132932 31.0 31.0 34.0 27.0 34.0 4 34.13187224994394 37.0 35.0 37.0 30.0 37.0 5 33.792198500496895 37.0 35.0 37.0 28.0 37.0 6 33.66216202067336 37.0 35.0 37.0 28.0 37.0 7 33.530343430359835 37.0 35.0 37.0 28.0 37.0 8 33.40773771870662 37.0 35.0 37.0 27.0 37.0 9 34.854795731310965 39.0 35.0 39.0 27.0 39.0 10 34.73140650180218 39.0 35.0 39.0 27.0 39.0 11 34.774536629459746 39.0 35.0 39.0 27.0 39.0 12 34.62198884403196 39.0 35.0 39.0 27.0 39.0 13 34.60733278481267 39.0 35.0 39.0 27.0 39.0 14 35.64457605838168 40.0 36.0 41.0 27.0 41.0 15 35.655180161118494 40.0 36.0 41.0 27.0 41.0 16 35.62526251774476 40.0 36.0 41.0 27.0 41.0 17 35.596952481997846 40.0 36.0 41.0 27.0 41.0 18 35.52127669784742 40.0 36.0 41.0 27.0 41.0 19 35.50660057079887 40.0 36.0 41.0 26.0 41.0 20 35.48176488705739 40.0 36.0 41.0 26.0 41.0 21 35.4268641050228 40.0 36.0 41.0 26.0 41.0 22 35.38334614475187 40.0 36.0 41.0 26.0 41.0 23 35.276024266368154 40.0 36.0 41.0 25.0 41.0 24 35.260851678673916 40.0 36.0 41.0 25.0 41.0 25 35.16523021726479 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5318946550715289 1101 2 0.5949155560434889 1101 3 0.6014858618595582 1101 4 0.3963723530858374 1101 5 0.6775168015955657 1101 6 0.724968564887952 1101 7 0.6878507438805954 1101 8 0.7145800118016226 1101 9 0.4901891882323639 1101 10 0.7996047105318453 1101 11 0.9463448587309671 1101 12 1.0311988484607113 1101 13 1.0646935871200611 1101 14 1.0118410530766013 1101 15 1.008639060672877 1101 16 1.2508596996139048 1101 17 1.1191998605430555 1101 18 0.488681491776525 1101 19 0.9129521270793362 1101 20 0.9265362661491352 1101 21 1.1369428058466298 1101 22 1.064247547403859 1101 23 1.0364546908450691 1101 24 1.0129591718798707 1101 25 1.0404716947802015 1102 1 0.6860751792935922 1102 2 0.6066148996014391 1102 3 0.5505626636036176 1102 4 0.48851943286020827 1102 5 0.7013286662418778 1102 6 0.5990963750525964 1102 7 0.6214848469683503 1102 8 0.628383894591245 1102 9 0.8183213409269712 1102 10 0.9554951565136207 1102 11 0.8411722521711127 1102 12 0.8968268818777005 1102 13 0.8833251561528073 1102 14 0.9464480714131298 1102 15 0.9971497576225161 1102 16 0.8782326832309835 1102 17 0.947605176988084 1102 18 0.9888280122892752 1102 19 0.9243754899476855 1102 20 1.0016142814782043 1102 21 0.9838612053078037 1102 22 1.0232675890136278 1102 23 1.0503191894048953 1102 24 1.0304412551547841 1102 25 1.0298213635041833 1103 1 0.5758621150409198 1103 2 0.5009894926390679 1103 3 0.513685124701734 1103 4 0.499418137427746 1103 5 0.6664528427845013 1103 6 0.7063531758179167 1103 7 0.6040693695334696 1103 8 0.6110796538631718 1103 9 0.7681509376650837 1103 10 0.8499995257091868 1103 11 0.7644005408185279 1103 12 0.8523025983515353 1103 13 0.8480126429346839 1103 14 0.8280659641334296 1103 15 0.8268482290291885 1103 16 0.8427145223196106 1103 17 0.976905377362165 1103 18 0.9166737148250306 1103 19 0.9853522121088432 1103 20 0.9840865546774253 1103 21 0.6851999951599339 1103 22 0.9238222068208941 1103 23 0.9600477196494026 1103 24 0.937456404301166 1103 25 0.8776745534854982 1104 1 0.5007882284494336 1104 2 0.5399307747180409 1104 3 0.45925767756040514 1104 4 0.4286581826248508 1104 5 0.5510325224331467 1104 6 0.5326376412273746 1104 7 0.5737410866172112 1104 8 0.4677866983182213 1104 9 0.6697243591629771 1104 10 0.6846707724735666 1104 11 0.6184289713337776 1104 12 0.6390217085305281 1104 13 0.6524807930234644 1104 14 0.8237306647490215 1104 15 0.8802521218799839 1104 16 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25 -1.3319674066586984 2215 1 -1.2425160085534017 2215 2 -1.0337430578217557 2215 3 -1.0025034920639904 2215 4 -1.0136877991959494 2215 5 -1.6156858443249007 2215 6 -1.454115112103068 2215 7 -1.6101453174610825 2215 8 -2.315353060254374 2215 9 -1.8174443995538994 2215 10 -1.35248355831083 2215 11 -1.9863959697521665 2215 12 -2.6947324395000614 2215 13 -2.1629667133488724 2215 14 -2.1607857590474424 2215 15 -3.194771924674143 2215 16 -2.456123132125441 2215 17 -2.348811458225036 2215 18 -2.9532864237568006 2215 19 -3.702973847592361 2215 20 -3.113689962975485 2215 21 -2.202242701060648 2215 22 -3.2520913039507846 2215 23 -3.072550115487985 2215 24 -2.0353990705425957 2215 25 -2.1248025489291607 2216 1 -0.9979006741751384 2216 2 -0.8112868143525809 2216 3 -1.259249834801711 2216 4 -0.7468252077643953 2216 5 -0.5063043134994061 2216 6 -1.0300882051020253 2216 7 -2.209146101160144 2216 8 -1.3456364980809141 2216 9 -1.293959551069051 2216 10 -1.3919101518322563 2216 11 -1.7973351024585398 2216 12 -1.294663865832348 2216 13 -1.7505744719695642 2216 14 -1.755172514533342 2216 15 -1.8382149758758146 2216 16 -1.561406569951977 2216 17 -1.804088365226086 2216 18 -1.613879422711868 2216 19 -1.8456462613854612 2216 20 -2.2416843464655614 2216 21 -2.001001844216347 2216 22 -2.4797297574513024 2216 23 -1.5317357215485856 2216 24 -1.880680549121486 2216 25 -1.7904275489291592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58205.0 3 3.0 4 930.0 5 8080.0 6 9314.0 7 7544.0 8 6082.0 9 5350.0 10 4825.0 11 4296.0 12 3861.0 13 3713.0 14 3644.0 15 3761.0 16 3789.0 17 3975.0 18 4384.0 19 5305.0 20 6721.0 21 8626.0 22 11043.0 23 13505.0 24 16113.0 25 18789.0 26 21384.0 27 23944.0 28 26808.0 29 32438.0 30 41237.0 31 52528.0 32 66715.0 33 85741.0 34 111511.0 35 145015.0 36 198478.0 37 310676.0 38 963893.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.56252608637187 19.468292069568317 13.295492449577988 27.673689394481826 2 13.608960725932228 20.803586035402283 39.936960813183696 25.65049242548179 3 16.94200310091588 25.049449748846754 30.194404914698637 27.814142235538732 4 11.38521439493915 17.639229281440052 37.74844292227878 33.22711340134202 5 12.551174508731266 38.24524208593345 36.31182035646317 12.891763048872122 6 31.63017923916674 37.74618371850872 18.016821544499816 12.606815497824725 7 27.453896762790087 31.605381165919283 22.805669208863865 18.13505286242676 8 24.89291221158766 37.360017532187456 20.58984285641942 17.157227399805468 9 26.304753557704565 14.783133224272877 20.043699838155213 38.86841337986734 10 14.319937700282628 29.274359412240774 35.058309897417736 21.34739299005886 11 34.13935241578857 22.17388686647284 24.983377601558054 18.70338311618053 12 22.993248983071627 24.776533180683185 32.1595007921199 20.07071704412529 13 28.922010253439083 21.84804474830822 25.798642211168843 23.43130278708386 14 20.613654102639835 22.128701834540173 27.681622705734064 29.576021357085924 15 22.86626746506986 31.14383255347229 23.200418834462223 22.789481146995627 16 22.023411633910044 28.04853891088953 27.878850815846747 22.049198639353676 17 21.643322602787848 28.62385008830117 27.93140480251913 21.801422506391855 18 21.63630612845878 27.53659733659079 29.704320132625128 21.122776402325304 19 23.397606351070547 26.985418487192586 27.441933312363204 22.175041849373667 20 23.524584442214564 27.438157148466473 27.85083547838227 21.186422930936697 21 22.717429145046413 26.763641154549873 27.281000323703942 23.237929376699775 22 22.959193689608192 28.096050159256166 27.163809391685522 21.780946759450124 23 22.022881697913597 27.762632376839097 27.669796813140074 22.544689112107232 24 22.960898656020813 27.105826963606944 28.16178879629076 21.77148558408148 25 22.757569729110934 27.238460209514535 27.6916579848841 22.312312076490432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 126.0 2 284.0 3 442.0 4 442.0 5 442.0 6 1086.0 7 1730.0 8 1730.0 9 1730.0 10 2587.5 11 3445.0 12 3445.0 13 3445.0 14 6277.0 15 9109.0 16 9109.0 17 9109.0 18 17246.5 19 25384.0 20 25384.0 21 25384.0 22 41294.5 23 57205.0 24 57205.0 25 57205.0 26 83248.0 27 109291.0 28 109291.0 29 109291.0 30 142652.5 31 176014.0 32 176014.0 33 176014.0 34 199500.0 35 222986.0 36 222986.0 37 222986.0 38 241253.5 39 259521.0 40 259521.0 41 259521.0 42 272543.5 43 285566.0 44 285566.0 45 285566.0 46 295395.0 47 305224.0 48 305224.0 49 305224.0 50 292073.5 51 278923.0 52 278923.0 53 278923.0 54 246972.0 55 215021.0 56 215021.0 57 215021.0 58 184311.5 59 153602.0 60 153602.0 61 153602.0 62 125236.0 63 96870.0 64 96870.0 65 96870.0 66 74873.5 67 52877.0 68 52877.0 69 52877.0 70 38545.5 71 24214.0 72 24214.0 73 24214.0 74 16890.5 75 9567.0 76 9567.0 77 9567.0 78 6577.0 79 3587.0 80 3587.0 81 3587.0 82 2350.5 83 1114.0 84 1114.0 85 1114.0 86 721.0 87 328.0 88 328.0 89 328.0 90 196.5 91 65.0 92 65.0 93 65.0 94 40.0 95 15.0 96 15.0 97 15.0 98 7.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07669400835694212 2 4.3625715788931806E-5 3 0.0 4 0.032893789704854584 5 0.07761014838850969 6 0.07464359971486233 7 0.03930676992582756 8 0.06901588237809012 9 0.049820567430960125 10 0.11556452112488037 11 0.00602034877887259 12 0.011997071841956246 13 0.0051042087473050215 14 0.006674734515706567 15 0.006587483084128703 16 0.016316017705060495 17 0.02778958095754956 18 0.0022249115052355222 19 0.0033591801157477493 20 0.005496840189405407 21 3.4900572631145444E-4 22 3.0538001052252264E-4 23 4.3625715788931806E-5 24 1.3087714736679542E-4 25 0.0030538001052252263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2292226.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.42248513209476 #Duplication Level Percentage of deduplicated Percentage of total 1 82.55762033256273 58.13912790411023 2 11.80899665644906 16.632377829274812 3 3.089053652750969 6.5261650479929445 4 1.0400516201591061 2.9297207902906295 5 0.4922277500130865 1.7331950703450523 6 0.2623503025047695 1.1085216166525615 7 0.16189075026248081 0.7980524267368255 8 0.1127741722409357 0.6353469974337271 9 0.07692609834646877 0.48755943153668313 >10 0.3582628993598782 4.300049224064309 >50 0.019528665130829456 0.9458058325031422 >100 0.016469296227812208 2.316222385498736 >500 0.002358331478873227 1.2306932707383442 >1k 0.0014894725129704885 2.2171621728220465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 4058 0.17703315467148525 No Hit GTCCTACAGTGGACATTTCTAAATT 3899 0.1700966658610451 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3879 0.1692241515452665 No Hit CTTTAGGACGTGAAATATGGCGAGG 3684 0.16071713696642476 No Hit GTATCAACGCAGAGTACTTTTTTTT 3411 0.1488073165560464 No Hit CCCATGTACTCTGCGTTGATACCAC 3196 0.13942778766142605 No Hit GTACATGGGAAGCAGTGGTATCAAC 3137 0.1368538704298791 No Hit TATCAACGCAGAGTACTTTTTTTTT 2557 0.11155095527229864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.3625715788931806E-5 0.0 0.0 0.0 0.0 9 4.3625715788931806E-5 0.0 0.0 0.0 0.0 10 4.3625715788931806E-5 0.0 0.0 0.0 0.0 11 4.3625715788931806E-5 0.0 0.0 0.0 0.0 12 4.3625715788931806E-5 0.0 0.0 0.0 0.0 13 4.3625715788931806E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAACG 60 4.0336655E-4 12.688736 4 TAGGACC 685 0.0 12.364572 4 TCCAACG 245 0.0 12.005365 18 CGTGCGC 145 9.640644E-11 11.794039 10 CGGTAGG 65 7.963487E-4 11.703205 1 CCAACGT 100 1.960003E-6 11.385734 19 GGCGAGG 985 0.0 10.692185 19 GTCTTAG 315 0.0 10.565393 1 GTAGGAC 2255 0.0 10.499799 3 GACGTGG 1195 0.0 10.498225 7 GTAATAC 145 1.4235411E-8 10.492529 3 TGTAGGA 2330 0.0 10.242996 2 ATATCGA 75 0.0026165827 10.148103 6 CGTGGAA 1270 0.0 10.100991 9 GTGCGCC 160 6.2664185E-9 10.084858 11 GGACGTG 2275 0.0 10.036584 6 ACGTGGA 1295 0.0 9.980459 8 CTGTAGG 2300 0.0 9.963626 1 TGGCAAG 1735 0.0 9.89826 18 GTCCTAC 2210 0.0 9.896093 1 >>END_MODULE