FastQCFastQC Report
Thu 26 May 2016
SRR1512936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847545
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30580.1655169427537624No Hit
GTACATGGGAAGCAGTGGTATCAAC23060.12481428057232707No Hit
GTCCTACAGTGGACATTTCTAAATT20710.1120946986406285No Hit
GGTATCAACGCAGAGTACTTTTTTT19310.10451707536216981No Hit
GTCCTAAAGTGTGTATTTCTCATTT19110.1034345577509614No Hit
CCCATGTACTCTGCGTTGATACCAC18920.10240616602031345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT400.00529835314.2423876
ACCGTGC655.4795913E-513.1471758
GTCCTAC11800.011.0666091
TAGGACC5850.011.0401224
TCCAACG909.500024E-510.55734618
GGACGTG11050.010.311236
GGCGAGA7300.010.27532919
ATACACT5200.010.2283494
TTAGGAC7250.010.2182713
TGGCGAG12250.010.16090818
AGGACGT11700.010.06572155
ACTGTTC4450.010.0286188
CCGTGCA951.6526424E-49.9949279
AGGACCT9800.09.9820615
ACGTGAA7000.09.9021188
TTAGACT1356.463506E-79.8495214
GACGTGA6100.09.8062337
TCCTACA14450.09.793532
GTCCTAA7300.09.7929691
CTAGACA2151.0913936E-119.718634
Produced by FastQC (version 0.11.5)