##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512936_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847545 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.862193884316756 31.0 30.0 34.0 22.0 34.0 2 28.971744125312238 31.0 30.0 34.0 20.0 34.0 3 29.206994687544825 31.0 30.0 34.0 22.0 34.0 4 32.60597062588462 35.0 33.0 37.0 25.0 37.0 5 32.73265062555986 35.0 33.0 37.0 25.0 37.0 6 32.76123450308382 37.0 35.0 37.0 25.0 37.0 7 32.58258445667088 36.0 33.0 37.0 25.0 37.0 8 32.57320335905215 37.0 33.0 37.0 25.0 37.0 9 33.88058856482522 39.0 34.0 39.0 23.0 39.0 10 33.75802267333137 38.0 34.0 39.0 22.0 39.0 11 33.813179651916464 38.0 34.0 39.0 23.0 39.0 12 33.710094206095114 38.0 34.0 39.0 22.0 39.0 13 33.6967456814313 38.0 34.0 39.0 22.0 39.0 14 34.69026410723419 39.0 34.0 41.0 19.0 41.0 15 34.62799661171988 39.0 34.0 41.0 19.0 41.0 16 34.5108465558349 39.0 34.0 41.0 18.0 41.0 17 34.45364199518821 39.0 34.0 41.0 17.0 41.0 18 34.147342013320376 39.0 33.0 41.0 17.0 41.0 19 34.38306184693742 39.0 34.0 41.0 18.0 41.0 20 34.45701078999429 39.0 34.0 41.0 18.0 41.0 21 34.38879486020638 39.0 34.0 41.0 18.0 41.0 22 34.300706613370714 39.0 34.0 41.0 16.0 41.0 23 34.0335520921006 39.0 34.0 41.0 10.0 41.0 24 34.03254968079262 39.0 34.0 41.0 2.0 41.0 25 33.95041419830099 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5901447349774855 1101 2 0.8231874065769489 1101 3 0.8533414202877836 1101 4 0.8270880690712374 1101 5 0.6966926696196794 1101 6 0.8900780445761427 1101 7 0.9520889927536942 1101 8 0.8074283562547464 1101 9 0.7088062999410596 1101 10 0.8639719367129501 1101 11 0.8828632122495392 1101 12 1.1197008703064384 1101 13 0.9378966805724289 1101 14 0.8436419038536442 1101 15 0.6457525016908221 1101 16 1.1542485270673453 1101 17 1.0784476605407889 1101 18 1.2591974540384072 1101 19 0.7432781362177678 1101 20 0.94361543341752 1101 21 0.9609188421095283 1101 22 0.9516407002229315 1101 23 1.1830383785076322 1101 24 0.9229463161994786 1101 25 1.0638343064840043 1102 1 0.6617542698001841 1102 2 0.6978133623485014 1102 3 0.8104499210829061 1102 4 0.9671924289577873 1102 5 0.8680585279964177 1102 6 0.7525416440022639 1102 7 0.9595024489766644 1102 8 1.0028463097211073 1102 9 1.0308144070001006 1102 10 1.0584485450115864 1102 11 1.0827289111468659 1102 12 1.1401605750510981 1102 13 1.0521028734240616 1102 14 1.2097966370078552 1102 15 1.1968433635336808 1102 16 1.089545220628743 1102 17 1.0008069505884905 1102 18 1.3578889400606613 1102 19 1.2157738108282672 1102 20 1.2129222270885478 1102 21 1.1824080792070362 1102 22 1.3642752822770419 1102 23 1.5480811753041053 1102 24 1.4777122754409362 1102 25 1.3667665051354874 1103 1 0.35066762036504073 1103 2 0.5057948008407855 1103 3 0.4650965246448351 1103 4 0.6506423079989858 1103 5 0.15110322146798438 1103 6 0.6492306225666837 1103 7 0.31959700046379425 1103 8 0.4988520534525307 1103 9 0.7745980286703613 1103 10 0.6787547136032686 1103 11 0.7317590178396784 1103 12 0.9427884599043139 1103 13 0.8757869721256597 1103 14 1.1280517501545049 1103 15 1.1787097372824888 1103 16 1.2058337984288698 1103 17 1.0755509892933404 1103 18 1.338022050004831 1103 19 1.3759164314327492 1103 20 1.1462594249463933 1103 21 0.9549932569706598 1103 22 1.1673128722285568 1103 23 1.4136891833193914 1103 24 1.2876544279303204 1103 25 1.2017082830443755 1104 1 0.697071282484977 1104 2 0.6237945040282504 1104 3 0.6142829019257903 1104 4 0.9497037603984069 1104 5 0.6456007173562952 1104 6 0.6658469987101796 1104 7 0.8750731185268421 1104 8 0.8296054447066155 1104 9 0.8673654931346277 1104 10 0.9456166435389122 1104 11 1.0325990266073646 1104 12 0.8044951759225754 1104 13 0.8437051006835645 1104 14 1.0675095595705884 1104 15 1.1538410695070098 1104 16 1.042044415237541 1104 17 1.0112758196635099 1104 18 1.3071940650739222 1104 19 1.1144203156250896 1104 20 1.189224259983547 1104 21 1.2758745802859224 1104 22 1.2291194451616931 1104 23 1.4509899271569893 1104 24 1.3820758411973557 1104 25 0.9055362372200335 1105 1 0.7708945122900452 1105 2 0.7795922935062016 1105 3 0.7896751586574311 1105 4 0.9182701416645571 1105 5 0.5544516323635591 1105 6 0.39385998606397266 1105 7 0.7718205545741412 1105 8 0.8171073830286133 1105 9 0.8322150674194759 1105 10 0.9130176459794086 1105 11 0.8418314033837575 1105 12 0.9824647448636199 1105 13 0.8890561099230965 1105 14 1.0703949755165922 1105 15 1.1246411134025465 1105 16 1.0447405316752238 1105 17 1.056041992425854 1105 18 1.068399474721879 1105 19 1.063037367610292 1105 20 1.0707294475296223 1105 21 1.1486964431266742 1105 22 0.7450382575011147 1105 23 1.0549734472451604 1105 24 1.0346043673330954 1105 25 1.1182549694023862 1106 1 0.8043990579508247 1106 2 0.8287048746955534 1106 3 0.893060967380837 1106 4 0.9275771235803134 1106 5 1.0014262298122887 1106 6 0.8643647786146147 1106 7 0.7977330306437977 1106 8 0.8021753189880982 1106 9 1.0746673967575902 1106 10 1.1117952170663088 1106 11 0.9286161961465282 1106 12 1.1149915713035767 1106 13 1.008367316660653 1106 14 1.3496963537984215 1106 15 1.3647552366223863 1106 16 1.375616201120927 1106 17 1.3177989879824281 1106 18 1.570562736684991 1106 19 1.2209627385195958 1106 20 1.2827295913031946 1106 21 1.2373703263625018 1106 22 1.312927254874893 1106 23 1.6239538274555585 1106 24 1.4177162756149784 1106 25 1.561823429487248 1107 1 0.6369416931788834 1107 2 0.6589174512441964 1107 3 0.862855443477855 1107 4 0.9977653101185879 1107 5 0.855364069848477 1107 6 0.8774370441789472 1107 7 1.0312832600766342 1107 8 0.9390155609171984 1107 9 1.0501768511560456 1107 10 0.9348292745724578 1107 11 0.9707382391438486 1107 12 1.1256317250045456 1107 13 1.088707459689246 1107 14 1.2289392966572734 1107 15 1.3030699252048805 1107 16 1.2936498627914972 1107 17 1.2738092783062172 1107 18 1.6362507748141155 1107 19 1.5828826788476746 1107 20 1.4441509889834379 1107 21 1.3885416844007992 1107 22 1.4296599836579276 1107 23 1.3727716335643123 1107 24 1.4059124353793564 1107 25 1.45661745603649 1108 1 0.48901798570927113 1108 2 0.565177863291332 1108 3 0.42880555277104193 1108 4 0.6541460906438772 1108 5 0.6217899068785968 1108 6 0.6266352441430811 1108 7 0.6774789442353111 1108 8 0.5804745890627956 1108 9 0.6879754727226484 1108 10 0.6299409009857797 1108 11 0.5195767933875146 1108 12 0.5897477847777495 1108 13 0.6780827345441196 1108 14 0.8923206800676056 1108 15 0.7473002782656692 1108 16 0.7041302384430992 1108 17 0.7610933159399593 1108 18 1.0548388189688396 1108 19 1.0200334191080245 1108 20 1.0308081837282828 1108 21 0.856564216823827 1108 22 0.7366561378908401 1108 23 0.9713217900317517 1108 24 1.0877283844459953 1108 25 1.1628759681384437 1109 1 0.3359468192600801 1109 2 0.31752230899066447 1109 3 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-3.948673765480912 2216 25 -3.984749079965674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93541.0 3 2.0 4 776.0 5 4609.0 6 5761.0 7 5086.0 8 4496.0 9 4430.0 10 4025.0 11 3786.0 12 3654.0 13 3545.0 14 3553.0 15 3680.0 16 3706.0 17 4075.0 18 4385.0 19 5501.0 20 6828.0 21 8920.0 22 10859.0 23 13311.0 24 15389.0 25 17272.0 26 19563.0 27 21617.0 28 24437.0 29 28850.0 30 35570.0 31 44267.0 32 55485.0 33 70665.0 34 91425.0 35 119820.0 36 162294.0 37 250274.0 38 692088.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.622213277841965 18.49373739480621 12.432869619724423 25.451179707627404 2 14.887257617278557 20.20580229486236 40.99855914568873 23.908380942170357 3 18.542083582399876 24.814499571172387 29.82940024776706 26.81401659866067 4 12.267739310336614 16.829813834092892 38.655888972933575 32.246557882636914 5 13.182493300621124 37.725801150414256 36.06900103042376 13.02270451854086 6 31.5510849723587 37.22052840026937 18.341348912645707 12.887037714726219 7 27.591571849431425 31.734946985715972 22.553726157647255 18.119755007205352 8 25.005061950697144 36.450646792780525 21.004171913590607 17.54011934293173 9 26.47029844476108 15.096774752378767 20.222382707440982 38.210544095419166 10 14.776514355874983 28.834964280842012 34.87062497157842 21.51789639170458 11 34.00627649605432 22.51412807105206 24.827618314982224 18.6519771179114 12 22.95283069428978 24.849758850842655 31.868176485778182 20.329233969089383 13 28.753643367879462 21.89734472648214 25.90061022351764 23.448401682120753 14 21.0323886310886 21.9180232989377 27.835933985079887 29.213654084893804 15 23.170670303498213 30.312139116386373 23.48046511451634 23.03672546559908 16 22.363260261469257 28.016860322535415 27.46840178282861 22.15147763316672 17 21.945746309017686 28.2376997483132 27.73053650054111 22.086017442128007 18 21.78699393762729 27.479144170039017 29.228449427219832 21.50541246511386 19 23.310374772074653 27.132753251590604 27.629370644514417 21.927501331820327 20 23.468749154102266 27.315215331727256 27.868500121809276 21.347535392361205 21 23.11356737234248 26.757877742653097 27.299708565615504 22.828846319388912 22 23.04826434791647 27.728369718092555 27.153933354338072 22.069432579652904 23 22.422489674583588 27.573585340805064 27.454482359495024 22.549442625116328 24 22.9551221218127 27.028880805841016 27.973059955727898 22.042937116618386 25 22.74866943021675 27.09809375678413 27.68272698186007 22.47050983113904 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 272.0 1 272.0 2 421.0 3 570.0 4 570.0 5 570.0 6 1144.0 7 1718.0 8 1718.0 9 1718.0 10 2565.5 11 3413.0 12 3413.0 13 3413.0 14 6031.0 15 8649.0 16 8649.0 17 8649.0 18 15427.5 19 22206.0 20 22206.0 21 22206.0 22 34468.0 23 46730.0 24 46730.0 25 46730.0 26 66453.5 27 86177.0 28 86177.0 29 86177.0 30 109876.0 31 133575.0 32 133575.0 33 133575.0 34 153165.5 35 172756.0 36 172756.0 37 172756.0 38 188660.5 39 204565.0 40 204565.0 41 204565.0 42 215120.5 43 225676.0 44 225676.0 45 225676.0 46 234360.5 47 243045.0 48 243045.0 49 243045.0 50 234491.0 51 225937.0 52 225937.0 53 225937.0 54 203446.5 55 180956.0 56 180956.0 57 180956.0 58 155669.0 59 130382.0 60 130382.0 61 130382.0 62 106387.5 63 82393.0 64 82393.0 65 82393.0 66 63754.0 67 45115.0 68 45115.0 69 45115.0 70 32942.0 71 20769.0 72 20769.0 73 20769.0 74 14468.5 75 8168.0 76 8168.0 77 8168.0 78 5645.0 79 3122.0 80 3122.0 81 3122.0 82 2039.0 83 956.0 84 956.0 85 956.0 86 625.5 87 295.0 88 295.0 89 295.0 90 181.0 91 67.0 92 67.0 93 67.0 94 42.0 95 17.0 96 17.0 97 17.0 98 16.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3538750071040218 2 0.03918713752574362 3 0.03512769648371217 4 0.034857067080910076 5 0.03962014457022698 6 0.016724897093169586 7 0.016616645332048745 8 0.023328254521540748 9 0.019972449926794745 10 0.019322939360069714 11 0.018186295868300905 12 0.01883580643502594 13 0.019106435837828038 14 0.02749594732469304 15 0.027928954369176393 16 0.019918324046234327 17 0.0220833592686511 18 0.10273092130367596 19 0.024302520371628295 20 0.02137972282136565 21 0.02565566738563878 22 0.018565177032223844 23 0.021866855746409425 24 0.017969792346059228 25 0.021866855746409425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1847545.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.57894540231226 #Duplication Level Percentage of deduplicated Percentage of total 1 81.9078445663638 58.62877134236834 2 12.142399599836667 17.38280316019534 3 3.262594445268938 7.005992090033778 4 1.1764249615307365 3.368290323653035 5 0.5238113380615012 1.874693158410816 6 0.2743271516649109 1.1781628926836696 7 0.17105143436823608 0.857057689714182 8 0.10201990091326374 0.5841981533935849 9 0.07790201276608284 0.5018529526662309 >10 0.3293621987481481 4.066922297804219 >50 0.017789600325452275 0.8692288670034317 >100 0.012048792064947059 1.7836411274047126 >500 0.0016664986850471206 0.8229664648743714 >1k 7.57499402294145E-4 1.0754194797943253 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3058 0.1655169427537624 No Hit GTACATGGGAAGCAGTGGTATCAAC 2306 0.12481428057232707 No Hit GTCCTACAGTGGACATTTCTAAATT 2071 0.1120946986406285 No Hit GGTATCAACGCAGAGTACTTTTTTT 1931 0.10451707536216981 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1911 0.1034345577509614 No Hit CCCATGTACTCTGCGTTGATACCAC 1892 0.10240616602031345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 5.412588056041937E-5 11 0.0 0.0 0.0 0.0 5.412588056041937E-5 12 0.0 0.0 0.0 0.0 1.623776416812581E-4 13 0.0 0.0 0.0 0.0 2.1650352224167747E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCGT 40 0.005298353 14.242387 6 ACCGTGC 65 5.4795913E-5 13.147175 8 GTCCTAC 1180 0.0 11.066609 1 TAGGACC 585 0.0 11.040122 4 TCCAACG 90 9.500024E-5 10.557346 18 GGACGTG 1105 0.0 10.31123 6 GGCGAGA 730 0.0 10.275329 19 ATACACT 520 0.0 10.228349 4 TTAGGAC 725 0.0 10.218271 3 TGGCGAG 1225 0.0 10.160908 18 AGGACGT 1170 0.0 10.0657215 5 ACTGTTC 445 0.0 10.028618 8 CCGTGCA 95 1.6526424E-4 9.994927 9 AGGACCT 980 0.0 9.982061 5 ACGTGAA 700 0.0 9.902118 8 TTAGACT 135 6.463506E-7 9.849521 4 GACGTGA 610 0.0 9.806233 7 TCCTACA 1445 0.0 9.79353 2 GTCCTAA 730 0.0 9.792969 1 CTAGACA 215 1.0913936E-11 9.71863 4 >>END_MODULE