FastQCFastQC Report
Thu 26 May 2016
SRR1512936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847545
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35040.18965708548370946No Hit
TATCAACGCAGAGTACTTTTTTTTT25980.1406190376959695No Hit
GTACATGGGAAGCAGTGGTATCAAC23150.12530141349737084No Hit
GGTATCAACGCAGAGTACTTTTTTT22900.12394826648336037No Hit
CCCATGTACTCTGCGTTGATACCAC22240.12037595836637267No Hit
GTCCTACAGTGGACATTTCTAAATT20040.1084682646430804No Hit
CTGTAGGACGTGGAATATGGCAAGA18610.10072826372294044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC453.5261124E-516.8963347
TACACCG500.001481982313.321035
TAGGACC5400.012.5104914
GGACGTG9950.011.2826156
TTAGGAC7300.010.9398853
AGGACGT10550.010.9129635
CCTAATA1053.4350815E-610.8654642
GGCGAGG5950.010.68597319
CCCGTTT1252.2670429E-710.63085712
TCCAACG1355.7807483E-810.54391818
TGTAGGA14250.010.5414062
GTAGGAC13000.010.5311423
GACGTGG5400.010.3842067
ATTATAC1855.820766E-1110.2781423
TAGGACG11700.010.2469464
GACGTGA5900.09.98745257
CTATTAG1356.36599E-79.8604731
CTGTAGG14200.09.8431121
CTAATAC1651.0091753E-89.7953813
TGGCGAG13150.09.74209818
Produced by FastQC (version 0.11.5)