##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847545 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.468940134069808 31.0 31.0 34.0 27.0 34.0 2 30.623842450386864 31.0 31.0 34.0 27.0 34.0 3 30.70879734999689 31.0 31.0 34.0 27.0 34.0 4 34.18671209632242 37.0 35.0 37.0 30.0 37.0 5 33.83953516693775 37.0 35.0 37.0 29.0 37.0 6 33.68988847362311 37.0 35.0 37.0 29.0 37.0 7 33.57366018148408 37.0 35.0 37.0 28.0 37.0 8 33.45762457747985 37.0 35.0 37.0 28.0 37.0 9 34.912907669366646 39.0 35.0 39.0 28.0 39.0 10 34.80758898971337 39.0 35.0 39.0 27.0 39.0 11 34.82450170361209 39.0 35.0 39.0 27.0 39.0 12 34.68284128397414 39.0 35.0 39.0 27.0 39.0 13 34.655759399635734 39.0 35.0 39.0 27.0 39.0 14 35.71709268245158 40.0 36.0 41.0 27.0 41.0 15 35.73203196674506 40.0 36.0 41.0 27.0 41.0 16 35.70598334546655 40.0 36.0 41.0 27.0 41.0 17 35.67777997288293 40.0 36.0 41.0 27.0 41.0 18 35.606384688870904 40.0 36.0 41.0 27.0 41.0 19 35.58991147712234 40.0 36.0 41.0 27.0 41.0 20 35.56328370892184 40.0 36.0 41.0 27.0 41.0 21 35.50937324936605 40.0 36.0 41.0 26.0 41.0 22 35.47030789507157 40.0 36.0 41.0 26.0 41.0 23 35.36295299979162 40.0 36.0 41.0 25.0 41.0 24 35.345719860679985 40.0 36.0 41.0 25.0 41.0 25 35.24759829936483 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5650888026555805 1101 2 0.5868702983695577 1101 3 0.6459898625139573 1101 4 0.4144132972277035 1101 5 0.7199639727029989 1101 6 0.619613078474984 1101 7 0.6960997343100033 1101 8 0.7712417733716208 1101 9 0.6045882171010035 1101 10 0.7932739374996061 1101 11 0.9673513792263577 1101 12 1.037614616649435 1101 13 1.0238946034435443 1101 14 1.0403360257475285 1101 15 0.9860794431400208 1101 16 1.2686007315310093 1101 17 1.1087259832773526 1101 18 0.6366868746904899 1101 19 0.9493143452387116 1101 20 0.8466572923472029 1101 21 1.1786151473322093 1101 22 1.087424924756938 1101 23 0.9578795316141822 1101 24 1.0340422569716452 1101 25 0.9845706260995897 1102 1 0.7135711976700279 1102 2 0.5566415647521836 1102 3 0.5622168349130341 1102 4 0.5713868655686056 1102 5 0.7573585464636707 1102 6 0.7435557224591278 1102 7 0.7444370194831649 1102 8 0.7041186226950273 1102 9 0.8666862387230267 1102 10 0.919863207200109 1102 11 0.838249330339039 1102 12 0.8787154260673802 1102 13 0.9220678256017649 1102 14 1.0155971464369387 1102 15 0.967872653282356 1102 16 0.9694454041487148 1102 17 1.048258288147423 1102 18 1.1635943203203851 1102 19 1.1411806936705204 1102 20 1.1672591269538017 1102 21 1.1533588122875784 1102 22 1.1584000775376992 1102 23 1.2732114747955734 1102 24 1.0433490102361773 1102 25 1.131647506283457 1103 1 0.5898818916854083 1103 2 0.48276557030236233 1103 3 0.5609508756344788 1103 4 0.6137983435993988 1103 5 0.6293177203822609 1103 6 0.8066227974226337 1103 7 0.780834208434591 1103 8 0.7354786250137408 1103 9 0.8111940411734508 1103 10 0.9575735157291732 1103 11 0.9175297231253836 1103 12 0.8678335554934193 1103 13 0.9929999857571588 1103 14 1.106986191639841 1103 15 0.8647312369848876 1103 16 0.8883628493082867 1103 17 1.1519688211791603 1103 18 1.1028448547423721 1103 19 1.164467933542788 1103 20 1.1515952757406183 1103 21 0.7682745832565345 1103 22 1.1037890652639817 1103 23 1.1210908947533724 1103 24 1.0719329411993925 1103 25 1.1889858330409595 1104 1 0.3814368671764079 1104 2 0.449181695619739 1104 3 0.4753647611888354 1104 4 0.40217246994292566 1104 5 0.5117315043954846 1104 6 0.7051843062066752 1104 7 0.6855388335842747 1104 8 0.5682739449439751 1104 9 0.7075049249669476 1104 10 0.7509225472562306 1104 11 0.7927904714737508 1104 12 0.6645307593533474 1104 13 0.8931486849322852 1104 14 0.8098479184932259 1104 15 0.8161688250705978 1104 16 0.8015067384605175 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2216 16 -2.1817512466257725 2216 17 -2.3368611376438224 2216 18 -2.187471748553996 2216 19 -2.3869561897585143 2216 20 -2.5786115989184566 2216 21 -2.5347919302077457 2216 22 -2.8345558737787897 2216 23 -1.9193863285570671 2216 24 -2.422475792218414 2216 25 -2.1792331304327135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46840.0 3 1.0 4 768.0 5 6784.0 6 7988.0 7 6242.0 8 4962.0 9 4509.0 10 3916.0 11 3541.0 12 3227.0 13 3061.0 14 2961.0 15 2982.0 16 2995.0 17 3186.0 18 3524.0 19 4285.0 20 5308.0 21 6845.0 22 8812.0 23 10724.0 24 12834.0 25 14833.0 26 16795.0 27 18357.0 28 21223.0 29 25057.0 30 31304.0 31 40167.0 32 51136.0 33 65874.0 34 86099.0 35 113228.0 36 156252.0 37 241202.0 38 809723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.708808283980716 19.278206827588782 13.35914313061971 27.65384175781079 2 13.751947181714948 20.62605342666094 39.687270979496006 25.934728412128116 3 16.85485333239515 24.74050699712321 30.384970325485984 28.019669344995656 4 11.39422808813744 17.418876987773007 37.77806894393493 33.408825980154624 5 12.747646421276645 37.93447298848302 36.137081437804916 13.180799152435421 6 31.586856108065888 37.57545127214244 18.01881738340974 12.818875236381935 7 27.294932000911814 31.82547920446725 22.607361780489402 18.27222701413153 8 25.03607128465768 36.77120839886825 20.63716187232715 17.55555844414692 9 26.115279480503727 14.940662106489805 20.06328003500275 38.88077837800372 10 14.416450430837136 29.057219007657704 34.961554362202286 21.56477619930288 11 33.94853901956093 22.504922980657746 24.68570327444521 18.860834725336108 12 22.6811674223429 25.001299156847207 31.912218137095692 20.405315283714202 13 28.63094683921423 21.830066491578673 25.847653957991874 23.691332711215228 14 20.807247658649437 21.838351273493345 27.776737297814595 29.577663770042623 15 22.97774217295284 30.436441778894036 23.470153293205733 23.115662754947387 16 22.18720378762083 28.02757133321893 27.463444922564566 22.321779956595666 17 21.85270162870823 28.289845531501136 27.71608373738839 22.14136910240224 18 21.669492979503865 27.570271266493062 29.264326916259048 21.495908837744025 19 23.284860307617695 27.038768316763083 27.607811255066338 22.06856012055288 20 23.33688054206257 27.321373863170123 27.835646957826466 21.50609863694084 21 22.88488008127551 26.747361225151455 27.376521141862465 22.99123755171057 22 22.9541985559176 27.681619883737298 27.164608073438195 22.199573486906914 23 22.20427648582308 27.438898646582356 27.548557680597767 22.808267186996797 24 22.769188301232177 27.084969513597773 27.957749337634535 22.18809284753551 25 22.66976927465442 27.035721129020214 27.714646669676174 22.579862926649195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 165.0 2 305.5 3 446.0 4 446.0 5 446.0 6 1049.5 7 1653.0 8 1653.0 9 1653.0 10 2531.5 11 3410.0 12 3410.0 13 3410.0 14 5967.0 15 8524.0 16 8524.0 17 8524.0 18 15369.5 19 22215.0 20 22215.0 21 22215.0 22 34676.0 23 47137.0 24 47137.0 25 47137.0 26 67128.0 27 87119.0 28 87119.0 29 87119.0 30 110642.5 31 134166.0 32 134166.0 33 134166.0 34 153313.5 35 172461.0 36 172461.0 37 172461.0 38 188183.5 39 203906.0 40 203906.0 41 203906.0 42 214828.5 43 225751.0 44 225751.0 45 225751.0 46 235229.5 47 244708.0 48 244708.0 49 244708.0 50 235925.5 51 227143.0 52 227143.0 53 227143.0 54 203805.5 55 180468.0 56 180468.0 57 180468.0 58 155393.5 59 130319.0 60 130319.0 61 130319.0 62 106012.5 63 81706.0 64 81706.0 65 81706.0 66 62957.5 67 44209.0 68 44209.0 69 44209.0 70 32155.0 71 20101.0 72 20101.0 73 20101.0 74 13989.5 75 7878.0 76 7878.0 77 7878.0 78 5378.0 79 2878.0 80 2878.0 81 2878.0 82 1866.5 83 855.0 84 855.0 85 855.0 86 560.5 87 266.0 88 266.0 89 266.0 90 161.0 91 56.0 92 56.0 93 56.0 94 30.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07988979970717898 2 1.623776416812581E-4 3 0.0 4 0.030635248397197362 5 0.07112140705639104 6 0.08043105851278319 7 0.03691385054220601 8 0.06500518255306366 9 0.045086858506829336 10 0.10565371885393861 11 0.004708951608756485 12 0.010446294948160937 13 0.005033706892119002 14 0.005791469219964872 15 0.005683217458844034 16 0.014289232467950712 17 0.023653009804903265 18 0.0014072728945709037 19 0.003085175191943904 20 0.004492448086514807 21 1.0825176112083874E-4 22 2.7062940280209684E-4 23 0.0 24 0.0 25 0.003626433997548098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1847545.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.268345308144 #Duplication Level Percentage of deduplicated Percentage of total 1 80.83733059558486 56.80305460079151 2 12.774872330206783 17.95338280332849 3 3.4936155230573704 7.364717458442624 4 1.275297466455101 3.584521709738729 5 0.5676581311907875 1.994419878974498 6 0.30511689978335166 1.2864035803996154 7 0.1758142121261316 0.8647921637430808 8 0.1113553036672477 0.6259802343986719 9 0.07248493883215935 0.45840570403480835 >10 0.3520495128160662 4.231985432795232 >50 0.01874261266654185 0.9007830460790213 >100 0.013194213043537127 1.890645491069868 >500 0.0015426628563430472 0.7385858956730427 >1k 9.255977138058276E-4 1.3023220005307008 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3504 0.18965708548370946 No Hit TATCAACGCAGAGTACTTTTTTTTT 2598 0.1406190376959695 No Hit GTACATGGGAAGCAGTGGTATCAAC 2315 0.12530141349737084 No Hit GGTATCAACGCAGAGTACTTTTTTT 2290 0.12394826648336037 No Hit CCCATGTACTCTGCGTTGATACCAC 2224 0.12037595836637267 No Hit GTCCTACAGTGGACATTTCTAAATT 2004 0.1084682646430804 No Hit CTGTAGGACGTGGAATATGGCAAGA 1861 0.10072826372294044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 5.412588056041937E-5 13 0.0 0.0 0.0 0.0 5.412588056041937E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGTC 45 3.5261124E-5 16.896334 7 TACACCG 50 0.0014819823 13.32103 5 TAGGACC 540 0.0 12.510491 4 GGACGTG 995 0.0 11.282615 6 TTAGGAC 730 0.0 10.939885 3 AGGACGT 1055 0.0 10.912963 5 CCTAATA 105 3.4350815E-6 10.865464 2 GGCGAGG 595 0.0 10.685973 19 CCCGTTT 125 2.2670429E-7 10.630857 12 TCCAACG 135 5.7807483E-8 10.543918 18 TGTAGGA 1425 0.0 10.541406 2 GTAGGAC 1300 0.0 10.531142 3 GACGTGG 540 0.0 10.384206 7 ATTATAC 185 5.820766E-11 10.278142 3 TAGGACG 1170 0.0 10.246946 4 GACGTGA 590 0.0 9.9874525 7 CTATTAG 135 6.36599E-7 9.860473 1 CTGTAGG 1420 0.0 9.843112 1 CTAATAC 165 1.0091753E-8 9.795381 3 TGGCGAG 1315 0.0 9.742098 18 >>END_MODULE