FastQCFastQC Report
Thu 26 May 2016
SRR1512935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215927
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35460.1600233220679201No Hit
GTATCAACGCAGAGTACTTTTTTTT31810.14355166032094016No Hit
GTCCTACAGTGGACATTTCTAAATT30370.13705325130295357No Hit
GTCCTAAAGTGTGTATTTCTCATTT29010.13091586500818844No Hit
CCCATGTACTCTGCGTTGATACCAC28970.1307353536465777No Hit
CTGTAGGACGTGGAATATGGCAAGA28620.12915587923248373No Hit
CTTTAGGACGTGAAATATGGCGAGG26050.11755802424899377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6950.012.983624
CTGGTCG550.003081527812.08441459
AGGACCT12750.011.84497455
GTCGTAC752.021128E-411.4320761
ACCGTGC752.0867004E-411.3928488
TGTAGGA19450.011.2322112
GTCCTAA9700.011.098151
GTAGGAC19000.010.9983133
CTGTAGG18800.010.8949311
AGGACGT16150.010.8216265
GGACGTG16150.010.69844156
TGCACCG803.7781737E-410.6856195
TCGAACT1803.6379788E-1110.54893419
GACGTGA8300.010.5228017
GACGTGG8950.010.1828627
GGCGAGG8300.010.18035619
GTATAAT3750.010.1618461
TAGGACG17800.010.1388944
TGGCGAG17500.010.09970118
GGACCTG13100.010.0731476
Produced by FastQC (version 0.11.5)