##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512935_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2215927
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.69830549472072	31.0	30.0	34.0	20.0	34.0
2	28.814115717710916	31.0	30.0	34.0	16.0	34.0
3	29.033001989686483	31.0	30.0	34.0	22.0	34.0
4	32.40859288234675	35.0	33.0	37.0	22.0	37.0
5	32.52802596836448	35.0	33.0	37.0	25.0	37.0
6	32.548599750804065	36.0	33.0	37.0	24.0	37.0
7	32.36323895146366	36.0	33.0	37.0	22.0	37.0
8	32.35725770749668	36.0	33.0	37.0	22.0	37.0
9	33.63755259085701	38.0	34.0	39.0	19.0	39.0
10	33.50687590340296	38.0	34.0	39.0	18.0	39.0
11	33.56462600076627	38.0	34.0	39.0	19.0	39.0
12	33.4581761944324	38.0	34.0	39.0	18.0	39.0
13	33.44457691972705	38.0	34.0	39.0	18.0	39.0
14	34.416060637376596	39.0	34.0	41.0	17.0	41.0
15	34.34335607626063	39.0	34.0	41.0	17.0	41.0
16	34.22448889336156	39.0	34.0	41.0	17.0	41.0
17	34.16825915294141	39.0	33.0	41.0	17.0	41.0
18	33.869493444504265	39.0	33.0	41.0	11.0	41.0
19	34.10005293495679	39.0	34.0	41.0	16.0	41.0
20	34.171984004888245	39.0	34.0	41.0	16.0	41.0
21	34.10645477039632	39.0	34.0	41.0	10.0	41.0
22	34.021193387688314	39.0	34.0	41.0	10.0	41.0
23	33.75157530008886	39.0	34.0	41.0	2.0	41.0
24	33.74020714581302	39.0	34.0	41.0	2.0	41.0
25	33.65813359375106	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5189079214124135
1101	2	0.6907638927667534
1101	3	0.7405186058490436
1101	4	0.7707411820057715
1101	5	0.6481725980626649
1101	6	0.8245348488940678
1101	7	0.8268310790592324
1101	8	0.6715885416436578
1101	9	0.5499758572701552
1101	10	0.6287341971382077
1101	11	0.9740531500839325
1101	12	1.1282836439985218
1101	13	0.9469644670283586
1101	14	0.9100627556176022
1101	15	0.5405724854532679
1101	16	1.0761826912535923
1101	17	1.1507644369392125
1101	18	1.2008155431531975
1101	19	0.7210388397442884
1101	20	0.8751972586715837
1101	21	0.9707428132842182
1101	22	0.9120082478454492
1101	23	1.1807843486082987
1101	24	0.868280730574611
1101	25	1.0470179873671626
1102	1	0.5987244908973501
1102	2	0.5597932902400302
1102	3	0.6640608799015233
1102	4	0.7445324842409491
1102	5	0.6021570489858945
1102	6	0.6104192026355619
1102	7	0.6820545970378546
1102	8	0.6771036048117836
1102	9	0.765579598766756
1102	10	0.9211115052042942
1102	11	0.8482028099478782
1102	12	0.8807192611025059
1102	13	0.7782205408864726
1102	14	0.7618484699033203
1102	15	0.8582595602832015
1102	16	0.9763770547132609
1102	17	0.8407364971918838
1102	18	1.2474749211901255
1102	19	1.204256769773444
1102	20	1.0304572197988904
1102	21	0.8344272156165786
1102	22	1.1585643702944282
1102	23	1.2419723952555302
1102	24	1.1874984176494436
1102	25	1.11714675315919
1103	1	0.44702946072581184
1103	2	0.5069668274289931
1103	3	0.6271465128257887
1103	4	0.7321960546525048
1103	5	0.15582763682235878
1103	6	0.6454097104666019
1103	7	0.5357319649950014
1103	8	0.4723914209349047
1103	9	0.7926322803044883
1103	10	0.7330046844028857
1103	11	0.7984994402278929
1103	12	0.9559345299342894
1103	13	0.8229473020228184
1103	14	1.1052870081093005
1103	15	1.2086095840136224
1103	16	1.1340038429700883
1103	17	1.0302425653999023
1103	18	1.3759539706172035
1103	19	1.390619404528337
1103	20	1.295850636301708
1103	21	1.1511622485001638
1103	22	1.217635324257408
1103	23	1.4437826874787305
1103	24	1.3208693241515803
1103	25	1.3530118965587477
1104	1	0.8696803546631706
1104	2	0.8398022219712686
1104	3	0.7606053110513571
1104	4	1.087505908127433
1104	5	0.8084808832264443
1104	6	0.8763446479581987
1104	7	1.0756818910603343
1104	8	1.022586339606768
1104	9	1.117077770289697
1104	10	1.18577555414344
1104	11	1.224810100262843
1104	12	1.0648873461730304
1104	13	1.116827527859627
1104	14	1.249792317962772
1104	15	1.4818469142999504
1104	16	1.130917070554446
1104	17	1.2221534405175234
1104	18	1.4673893113888141
1104	19	1.3560667092736054
1104	20	1.4361971210442803
1104	21	1.4045733252577932
1104	22	1.3015158861608924
1104	23	1.6459267928692398
1104	24	1.5384775376211337
1104	25	1.276432383183291
1105	1	0.8266498486554106
1105	2	0.9050866497665488
1105	3	0.8997103082810902
1105	4	1.0451497759154407
1105	5	0.7652684492786932
1105	6	0.6050938106762018
1105	7	0.9128774718652863
1105	8	0.997171006389209
1105	9	0.9061798732112933
1105	10	1.1841820307781035
1105	11	1.0010304646148924
1105	12	1.0062738851593593
1105	13	1.0466298052666545
1105	14	1.2401848688405792
1105	15	1.3467650053796945
1105	16	1.3957003695695533
1105	17	1.190194490076351
1105	18	1.2029231458509244
1105	19	1.2901132828262405
1105	20	1.2944620246638223
1105	21	1.2738250225627752
1105	22	0.9772557953623107
1105	23	1.191179300580508
1105	24	1.3715251613941533
1105	25	1.4116945661531801
1106	1	0.4286478105187399
1106	2	0.450903325252078
1106	3	0.5234523958033819
1106	4	0.5046249067285373
1106	5	0.44514748390741943
1106	6	0.4748365449149432
1106	7	0.25180661721970665
1106	8	0.5085666890670097
1106	9	0.5400810431801375
1106	10	0.633655103857059
1106	11	0.5823783294707567
1106	12	0.674063976679534
1106	13	0.6178793398267928
1106	14	0.7473996766874009
1106	15	0.7796951208087819
1106	16	0.7765903885790948
1106	17	0.586246335177961
1106	18	0.9131035405439363
1106	19	0.5654003857325449
1106	20	0.7244797113786987
1106	21	0.5568036417251818
1106	22	0.6647602047926071
1106	23	0.8568932853336761
1106	24	0.7445445922836811
1106	25	0.813945580322148
1107	1	0.38175448487532293
1107	2	0.5130057656391038
1107	3	0.6665640989261838
1107	4	0.8031177445267943
1107	5	0.7086934608299273
1107	6	0.5576370838541749
1107	7	0.7742237377333296
1107	8	0.7157337131341208
1107	9	0.8175907168619148
1107	10	0.6737101622346202
1107	11	0.7709556918296485
1107	12	0.9291712871315028
1107	13	0.7659782791087153
1107	14	1.0234679750096873
1107	15	1.0686083847522383
1107	16	0.99185432300321
1107	17	1.0653534888640266
1107	18	1.4333326316500816
1107	19	1.2051760556648432
1107	20	1.113726941525229
1107	21	1.092182737798808
1107	22	1.1810070065747098
1107	23	1.230522470757414
1107	24	1.0950077452007534
1107	25	1.0950782074992915
1108	1	0.34918543403816216
1108	2	0.3851016840390784
1108	3	0.4059507923716481
1108	4	0.5049804044220636
1108	5	0.5805628928214617
1108	6	0.5574751930726833
1108	7	0.6817578294011071
1108	8	0.7371473241558064
1108	9	0.8709912146499548
1108	10	0.8111993832410818
1108	11	0.5662515876050875
1108	12	0.7148513525434126
1108	13	0.7351106481621699
1108	14	0.8893231845546055
1108	15	0.9476469672220915
1108	16	0.9140098437239033
1108	17	0.9940312396796429
1108	18	1.0862639434402368
1108	19	1.092417398987152
1108	20	0.9790481123482877
1108	21	0.8432262102540378
1108	22	0.8180399205372737
1108	23	0.9630353539293921
1108	24	0.9747956851489903
1108	25	1.0519411835435548
1109	1	0.29256606113889205
1109	2	0.2215154580987715
1109	3	0.5436616277636936
1109	4	0.5745607980874965
1109	5	0.4411945127108794
1109	6	0.45090245475994095
1109	7	0.5092375710677999
1109	8	0.5419254999563918
1109	9	0.6341011250243156
1109	10	0.8852025197613784
1109	11	0.39920556399066953
1109	12	0.663768279029
1109	13	0.5705700857464322
1109	14	0.41612429788156646
1109	15	0.7386446554977582
1109	16	0.6636023650054454
1109	17	0.4721157715907225
1109	18	-0.9451207725495578
1109	19	0.39426033422443396
1109	20	0.5832896132604759
1109	21	0.6877029797037224
1109	22	0.7676115185655021
1109	23	0.4175387562537125
1109	24	0.8377361598051252
1109	25	0.9504884932165609
1110	1	0.39515697471166433
1110	2	0.34454773731849286
1110	3	0.35546464390641574
1110	4	0.25856838545175975
1110	5	0.3611755328349844
1110	6	0.2494827803529347
1110	7	0.42866531175828015
1110	8	0.16818278570310952
1110	9	0.47792187166000843
1110	10	0.633637515324331
1110	11	0.49824135419261495
1110	12	0.07309039870835221
1110	13	0.24017070578628363
1110	14	0.4129987113120066
1110	15	0.448051421361626
1110	16	0.6665074096101193
1110	17	0.2593953893201686
1110	18	0.7017799945401109
1110	19	0.5628185188127333
1110	20	0.5812596935859062
1110	21	0.5223492461158017
1110	22	0.4847947431592772
1110	23	0.45556779081221777
1110	24	0.6720888343330174
1110	25	0.7924158383564617
1111	1	0.14479592795379403
1111	2	0.3976181391218745
1111	3	0.3135977676651116
1111	4	0.2794968641389275
1111	5	0.3577379841705479
1111	6	0.31327729581462904
1111	7	0.3362426318060585
1111	8	0.1293370310277666
1111	9	0.6062771350571694
1111	10	0.487627081998788
1111	11	0.29890486026742025
1111	12	0.4296262211923789
1111	13	0.37970949169712753
1111	14	0.45103855804567416
1111	15	0.5928120774483929
1111	16	0.45977102763587396
1111	17	0.4841999793750489
1111	18	0.7144076214280446
1111	19	0.34169928378220504
1111	20	0.2603093768599578
1111	21	0.4742238127852971
1111	22	0.3599387733734716
1111	23	0.6733275882557308
1111	24	0.6844360052572469
1111	25	0.17140334686602188
1112	1	3.934808666627987E-5
1112	2	0.273147453760874
1112	3	0.339166869935557
1112	4	0.3725707684220083
1112	5	0.31002993063713546
1112	6	0.3637731338494845
1112	7	-0.014569983357766603
1112	8	0.2651049521919404
1112	9	0.574889298742363
1112	10	0.512080573537375
1112	11	0.47329428079916624
1112	12	0.5413966680926166
1112	13	0.24806245602475485
1112	14	0.39867260194657206
1112	15	0.505645526621926
1112	16	0.5373285838735882
1112	17	0.5621842339416787
1112	18	0.4120539229672744
1112	19	0.4161334612582337
1112	20	0.18976517747257304
1112	21	0.15678791857732932
1112	22	0.272085794800482
1112	23	0.48412100199475105
1112	24	0.27619668580132384
1112	25	0.4384214212506734
1113	1	-0.4431240766616682
1113	2	-0.23660561598804364
1113	3	-0.3657260537920841
1113	4	-0.137165685526206
1113	5	-0.2845198922060135
1113	6	-0.1540993077022108
1113	7	-0.37571635689036853
1113	8	-0.05337212618941578
1113	9	-0.266292825509133
1113	10	-0.5571307206033111
1113	11	-0.38142826207648994
1113	12	-0.1017363781088747
1113	13	-0.3457707800183414
1113	14	-0.10278513061749095
1113	15	-0.5577321625238341
1113	16	-0.4401103198272338
1113	17	-0.43719084984653733
1113	18	-0.49230285345067415
1113	19	-0.5438467748547282
1113	20	-0.6578304026920208
1113	21	-0.428003728197659
1113	22	-0.2958779363918467
1113	23	-0.6751902414555815
1113	24	-0.03965995923022092
1113	25	-0.17215932611021145
1114	1	-1.4061877387888266
1114	2	-1.1192265497925113
1114	3	-1.0416880819572398
1114	4	-1.2827392192351503
1114	5	-1.2711076940484105
1114	6	-1.6436503698460676
1114	7	-1.7951331894962728
1114	8	-1.264037547938905
1114	9	-1.4106559805038685
1114	10	-1.639840096949559
1114	11	-2.323178109096016
1114	12	-1.696020996422078
1114	13	-1.6015274477542754
1114	14	-1.8127057449795174
1114	15	-1.8096574422053138
1114	16	-2.5343980991484756
1114	17	-4.331182381136532
1114	18	-3.500586500800736
1114	19	-2.542459543898591
1114	20	-1.6706041593300327
1114	21	-2.6031002559411682
1114	22	-2.682024872032656
1114	23	-2.4271291093664153
1114	24	-2.1036217663711234
1114	25	-1.8666333668037964
1115	1	-1.2363729200481117
1115	2	-0.7917071039823682
1115	3	-0.8999956045230633
1115	4	-0.8928998508796937
1115	5	-0.7590107206207968
1115	6	-0.9873611928510826
1115	7	-1.979652185210437
1115	8	-1.3049189995503774
1115	9	-1.0677939967892556
1115	10	-0.8430406518286233
1115	11	-1.6274006749268821
1115	12	-1.2322191778451312
1115	13	-1.1143555209727012
1115	14	-1.139214691956795
1115	15	-2.417435411523279
1115	16	-1.2712061441516553
1115	17	-1.2772920662070177
1115	18	-1.3145659463895853
1115	19	-1.3899561479079665
1115	20	-1.17860246367853
1115	21	-1.9097762629369512
1115	22	-1.9855747855143946
1115	23	-1.426467859336114
1115	24	-1.3203694584784458
1115	25	-1.4975732464307825
1116	1	-1.3587652253110676
1116	2	-2.1745645977843964
1116	3	-1.8121671163271493
1116	4	-1.4823255024144757
1116	5	-2.168411218562575
1116	6	-1.7856547198911947
1116	7	-1.442961204051194
1116	8	-2.1729403966922405
1116	9	-2.13782570701753
1116	10	-2.9344727405664486
1116	11	-1.7253984615466358
1116	12	-1.5570758166871634
1116	13	-2.2173256856524333
1116	14	-2.0716268920724588
1116	15	-1.5798244056975577
1116	16	-1.5336172850749676
1116	17	-1.9189689141476975
1116	18	-1.626237461147113
1116	19	-2.1347737317631896
1116	20	-2.2785985554287045
1116	21	-2.9018725356722683
1116	22	-1.9829164473847598
1116	23	-1.9661625287341877
1116	24	-2.6290710270298376
1116	25	-2.550794870170222
1201	1	0.14807776550663476
1201	2	0.2456158246427087
1201	3	0.3513430423852242
1201	4	0.42820284104661255
1201	5	0.3912924082643272
1201	6	0.3409854504870182
1201	7	0.2520513807030227
1201	8	-0.13066107706503516
1201	9	0.2234220951959287
1201	10	0.28520026221192296
1201	11	0.027349201600621598
1201	12	-0.6035912500872271
1201	13	-0.21513305881472178
1201	14	0.3544090528553596
1201	15	0.10690022651410658
1201	16	0.49690799205006186
1201	17	0.5428642928958283
1201	18	0.9218479952799541
1201	19	0.37711937694137276
1201	20	0.3263856165770278
1201	21	0.314172952243716
1201	22	0.5338361160033855
1201	23	0.7454365262053173
1201	24	0.5986442282868651
1201	25	0.47486284417109204
1202	1	0.701207692421626
1202	2	0.6970497587933515
1202	3	0.46986391291741114
1202	4	0.830232919025029
1202	5	0.7496017212762638
1202	6	0.7718931780890159
1202	7	0.7261537629415926
1202	8	0.8244205782821794
1202	9	1.1040311323349883
1202	10	1.1838599351506787
1202	11	0.8135112971898195
1202	12	0.9061622374984921
1202	13	0.9871695931112328
1202	14	1.1745202129922134
1202	15	0.8017847571522125
1202	16	0.9538160852832789
1202	17	1.2190047255227796
1202	18	1.6025529762492638
1202	19	1.3200135541955405
1202	20	1.210497015886176
1202	21	1.159528817257069
1202	22	1.400757006329144
1202	23	1.440553012736757
1202	24	1.551273336655285
1202	25	1.3240492459888031
1203	1	0.4540373752918647
1203	2	0.6630145587674221
1203	3	0.8451806105110506
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2211	18	0.1624340140709748
2211	19	-0.17292536358410615
2211	20	-0.2881060038098795
2211	21	-0.05970186458693405
2211	22	-0.24536425998120848
2211	23	0.42388247020988246
2211	24	0.1753947526540358
2211	25	0.15723589806084703
2212	1	-0.2355826396677081
2212	2	-0.3425042574474908
2212	3	-0.24698846828790977
2212	4	-0.4235343253109072
2212	5	-0.415749303465347
2212	6	-0.4671075635887547
2212	7	-0.464419224118064
2212	8	-0.40670598499552213
2212	9	-0.33684350969564747
2212	10	-0.30823473729464723
2212	11	-0.5302317557289911
2212	12	-0.41325316172547133
2212	13	-0.6058358806149471
2212	14	-0.4852774093108465
2212	15	-0.5878857569268732
2212	16	-0.3893116898604845
2212	17	-0.4266746244238675
2212	18	-2.945998063443941
2212	19	-1.4605113057808126
2212	20	-0.768435007477656
2212	21	-0.8653711611680421
2212	22	-0.590919939558546
2212	23	-0.6082355802460953
2212	24	-1.0925588684228842
2212	25	-0.7963474827597707
2213	1	-0.22566085214017306
2213	2	-0.10833725488654977
2213	3	-0.4816756387552701
2213	4	-0.5330122438249916
2213	5	-0.4105564187238997
2213	6	-0.47975257830702844
2213	7	-0.26976535807677493
2213	8	-0.6483480804872102
2213	9	-0.4006720529730785
2213	10	-0.5420233396894716
2213	11	-0.6156556543011291
2213	12	-0.5922786204756179
2213	13	-0.39980240518815435
2213	14	-0.48018647357046973
2213	15	-0.7011883915594055
2213	16	-1.2966984324189
2213	17	-0.801118485294424
2213	18	-0.5650845938800373
2213	19	-0.44872349324646166
2213	20	-1.1462938341684321
2213	21	-0.7720308249480539
2213	22	-0.7798763873035739
2213	23	-1.1358626195314727
2213	24	-0.9378018319467998
2213	25	-1.0780496727904278
2214	1	-1.8422606249535036
2214	2	-3.8720587889375047
2214	3	-2.099143048286294
2214	4	-1.8007030535832023
2214	5	-1.7315517127142748
2214	6	-1.8770566298026736
2214	7	-2.050675186604927
2214	8	-1.7977222352987
2214	9	-1.9774708845844806
2214	10	-1.9104919933379811
2214	11	-1.7506586293646897
2214	12	-2.2614688622671473
2214	13	-2.06705182369846
2214	14	-3.2939410037808905
2214	15	-3.473813479459011
2214	16	-2.6869250781699705
2214	17	-3.0106797986497575
2214	18	-2.4755816999545743
2214	19	-4.65361341589481
2214	20	-3.0232300596241544
2214	21	-2.9135335025052562
2214	22	-3.033358293507934
2214	23	-3.1482194107901975
2214	24	-3.3437368132850374
2214	25	-3.226597300853392
2215	1	-0.753197830900433
2215	2	-2.576514684337507
2215	3	-1.1953872344370104
2215	4	-0.7168393418605099
2215	5	-0.6001190189417009
2215	6	-1.206004625536945
2215	7	-1.786482673140096
2215	8	-1.6421594009183522
2215	9	-1.2594064147089554
2215	10	-2.8651676275964526
2215	11	-1.8092915346314342
2215	12	-2.0954070450303703
2215	13	-1.376774820104849
2215	14	-2.66317281393799
2215	15	-2.712273873590945
2215	16	-2.215204447630569
2215	17	-2.4382463610629443
2215	18	-1.2612743881709392
2215	19	-2.0136953250777765
2215	20	-2.1685460014453355
2215	21	-1.9141445951952676
2215	22	-1.5037449872984325
2215	23	-2.1292084148856567
2215	24	-1.8774270280950063
2215	25	-1.8028720461194148
2216	1	-2.4168666142223465
2216	2	-2.3745197918659713
2216	3	-2.879093650903787
2216	4	-3.7021327824217067
2216	5	-2.862781995799274
2216	6	-2.7634182789152923
2216	7	-2.5184841222463845
2216	8	-3.2233532311735154
2216	9	-3.585893072788071
2216	10	-3.064457388964719
2216	11	-3.6496692404116224
2216	12	-4.142294772526384
2216	13	-4.4293838518519095
2216	14	-3.954399818549444
2216	15	-3.72563085327403
2216	16	-2.816330759087336
2216	17	-2.9713834165385116
2216	18	-3.067466028842432
2216	19	-3.3693088118054426
2216	20	-3.2439141544094774
2216	21	-3.515924493051621
2216	22	-4.1110652242096535
2216	23	-4.057099452893713
2216	24	-4.009993684041653
2216	25	-4.3019386603514675
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121992.0
3	3.0
4	992.0
5	5909.0
6	7253.0
7	6729.0
8	5749.0
9	5382.0
10	5023.0
11	4825.0
12	4645.0
13	4512.0
14	4467.0
15	4482.0
16	4678.0
17	4880.0
18	5509.0
19	6679.0
20	8377.0
21	10931.0
22	13648.0
23	16518.0
24	19097.0
25	21634.0
26	23910.0
27	26285.0
28	29650.0
29	35555.0
30	43226.0
31	53704.0
32	67498.0
33	86026.0
34	110022.0
35	143130.0
36	194606.0
37	298322.0
38	810079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.555169071623325	18.50190675471254	12.67036241926864	25.2725617543955
2	14.692343878524882	20.018257023130076	41.28055590919885	24.00884318914619
3	18.524805303210538	24.836633468932256	29.698872878153736	26.939688349703466
4	12.284803534966619	16.880567118725022	38.40285455416326	32.4317747921451
5	13.166056738241835	37.90824798398565	35.915935520046375	13.009759757726137
6	31.728572763303458	36.97990506122003	18.331480506045274	12.960041669431238
7	27.76117786183053	31.60421387111043	22.540419040053443	18.094189227005604
8	25.037431248152135	36.660730930601545	20.87686394525581	17.424973875990514
9	26.497435534024145	15.214823394212788	20.18311230433619	38.10462876742688
10	14.966578393966461	28.88593593360906	34.59552612229478	21.551959550129702
11	33.99006996163394	22.514105168133604	24.80749266531257	18.688332204919885
12	23.271646452929147	24.793386564597448	31.752524272282876	20.182442710190525
13	28.852010314606325	21.9596578468327	25.7139362283621	23.474395610198876
14	21.023368476932163	22.070408776811366	27.659017411700127	29.247205334556348
15	23.218700669306212	30.565495361983753	23.311646072571445	22.904157896138592
16	22.25394376515205	28.0189342253682	27.648627214495956	22.078494794983794
17	21.954072017784398	28.476071182730177	27.673426092059085	21.89643070742634
18	21.84229262439645	27.4343503339405	29.40433725099026	21.319019790672797
19	23.498233694057575	27.098825562279327	27.408843175685377	21.99409756797773
20	23.568851831522995	27.40161702680841	27.79752318292119	21.232007958747403
21	23.030807502425137	26.84972986051332	27.09732198758022	23.022140649481322
22	23.224381872657958	27.85357768648086	26.954089448706874	21.967950992154304
23	22.501400404706803	27.666083935812036	27.380044785867852	22.452470873613308
24	23.055413877617344	27.091414617853225	27.898587684099823	21.954583820429608
25	22.868000462212336	27.144999259738057	27.48344340968263	22.503556868366985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	250.0
1	250.0
2	412.5
3	575.0
4	575.0
5	575.0
6	1177.5
7	1780.0
8	1780.0
9	1780.0
10	2742.0
11	3704.0
12	3704.0
13	3704.0
14	6554.5
15	9405.0
16	9405.0
17	9405.0
18	17250.5
19	25096.0
20	25096.0
21	25096.0
22	39883.0
23	54670.0
24	54670.0
25	54670.0
26	78287.0
27	101904.0
28	101904.0
29	101904.0
30	131821.0
31	161738.0
32	161738.0
33	161738.0
34	184492.0
35	207246.0
36	207246.0
37	207246.0
38	226244.0
39	245242.0
40	245242.0
41	245242.0
42	259464.0
43	273686.0
44	273686.0
45	273686.0
46	285576.0
47	297466.0
48	297466.0
49	297466.0
50	285197.0
51	272928.0
52	272928.0
53	272928.0
54	243954.0
55	214980.0
56	214980.0
57	214980.0
58	184367.5
59	153755.0
60	153755.0
61	153755.0
62	125853.0
63	97951.0
64	97951.0
65	97951.0
66	75788.0
67	53625.0
68	53625.0
69	53625.0
70	39342.0
71	25059.0
72	25059.0
73	25059.0
74	17425.5
75	9792.0
76	9792.0
77	9792.0
78	6686.0
79	3580.0
80	3580.0
81	3580.0
82	2329.5
83	1079.0
84	1079.0
85	1079.0
86	686.5
87	294.0
88	294.0
89	294.0
90	188.5
91	83.0
92	83.0
93	83.0
94	50.0
95	17.0
96	17.0
97	17.0
98	19.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36043606129624306
2	0.04011865011798674
3	0.036824317768590756
4	0.03610227232214779
5	0.04120171828765117
6	0.01778036911865779
7	0.017464474235838995
8	0.024008011094228288
9	0.020578295223624244
10	0.020036761138792027
11	0.019269587851946386
12	0.019720866255973232
13	0.01931471569234907
14	0.028656178655704814
15	0.029242840580939716
16	0.02111982930845646
17	0.022654175882147742
18	0.1064565755099333
19	0.02567774118912762
20	0.022699303722550426
21	0.026625425837584
22	0.02008188897919471
23	0.022383408839731632
24	0.018818309447919537
25	0.022338280999328948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2215927.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.51543909378717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.73188945911555	60.71854730280213
2	11.075721096184015	16.06321557140211
3	2.7784275346767155	6.0443667800205185
4	1.005643728727415	2.9169878624232757
5	0.46591297428777245	1.6892941954985097
6	0.25014545829988377	1.088364464755962
7	0.15261135761525407	0.774667572571839
8	0.10437598726081823	0.6055096437652616
9	0.07187212525923134	0.46906548495991496
>10	0.3286420201362619	4.126811323576482
>50	0.018906620110078317	0.9271729888570479
>100	0.012609159018284261	1.8661257396476276
>500	0.0021200826249094602	1.0602545777208414
>1k	0.001122396683774334	1.6496164919984335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3546	0.1600233220679201	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3181	0.14355166032094016	No Hit
GTCCTACAGTGGACATTTCTAAATT	3037	0.13705325130295357	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2901	0.13091586500818844	No Hit
CCCATGTACTCTGCGTTGATACCAC	2897	0.1307353536465777	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2862	0.12915587923248373	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2605	0.11755802424899377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.025568080536949E-5
2	0.0	0.0	0.0	0.0	9.025568080536949E-5
3	0.0	0.0	0.0	0.0	9.025568080536949E-5
4	0.0	0.0	0.0	0.0	9.025568080536949E-5
5	0.0	0.0	0.0	0.0	9.025568080536949E-5
6	0.0	0.0	0.0	0.0	1.8051136161073897E-4
7	0.0	0.0	0.0	0.0	1.8051136161073897E-4
8	0.0	0.0	0.0	0.0	1.8051136161073897E-4
9	0.0	0.0	0.0	0.0	1.8051136161073897E-4
10	0.0	0.0	0.0	0.0	1.8051136161073897E-4
11	0.0	0.0	0.0	0.0	1.8051136161073897E-4
12	0.0	0.0	0.0	0.0	2.707670424161085E-4
13	0.0	0.0	0.0	0.0	2.707670424161085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	695	0.0	12.98362	4
CTGGTCG	55	0.0030815278	12.0844145	9
AGGACCT	1275	0.0	11.8449745	5
GTCGTAC	75	2.021128E-4	11.432076	1
ACCGTGC	75	2.0867004E-4	11.392848	8
TGTAGGA	1945	0.0	11.232211	2
GTCCTAA	970	0.0	11.09815	1
GTAGGAC	1900	0.0	10.998313	3
CTGTAGG	1880	0.0	10.894931	1
AGGACGT	1615	0.0	10.821626	5
GGACGTG	1615	0.0	10.6984415	6
TGCACCG	80	3.7781737E-4	10.685619	5
TCGAACT	180	3.6379788E-11	10.548934	19
GACGTGA	830	0.0	10.522801	7
GACGTGG	895	0.0	10.182862	7
GGCGAGG	830	0.0	10.180356	19
GTATAAT	375	0.0	10.161846	1
TAGGACG	1780	0.0	10.138894	4
TGGCGAG	1750	0.0	10.099701	18
GGACCTG	1310	0.0	10.073147	6
>>END_MODULE