FastQCFastQC Report
Thu 26 May 2016
SRR1512935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215927
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42350.1911164041053699No Hit
GTACATGGGAAGCAGTGGTATCAAC35840.16173818000322213No Hit
CCCATGTACTCTGCGTTGATACCAC35710.16115151807798722No Hit
TATCAACGCAGAGTACTTTTTTTTT29720.13411994167677907No Hit
GTCCTACAGTGGACATTTCTAAATT29190.13172816613543678No Hit
CTGTAGGACGTGGAATATGGCAAGA28830.13010356388094013No Hit
CTTTAGGACGTGAAATATGGCGAGG27350.12342464350134279No Hit
GTCCTAAAGTGTGTATTTCTCATTT26820.12103286796000048No Hit
GGTATCAACGCAGAGTACTTTTTTT24440.11029244194416153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.012.4432434
ACTGCGC802.8565724E-511.8794928
CGTGCGC1052.7218812E-711.76183510
GTACCGT752.0491451E-411.4151466
TCCAACG1451.2423698E-911.12504818
AAGGCGT1553.0559022E-1011.0469176
GTGCGCC1658.185452E-1110.9304411
GGCGTGC1502.1209416E-910.7707398
AATCACG803.7557402E-410.6925097
TTTAGAC2050.010.6678723
TTATACT2500.010.657274
TGGCGAG17100.010.54334518
GGCGAGG7900.010.33027519
GCGTGCG1303.8338658E-710.2325579
GTCATAT2053.6379788E-1210.2049751
AGGACCT13300.010.1593215
CAGCGTG750.002644493510.1351049
GGACCTG12750.010.0721886
ATTTAGA5400.010.0374691
GTCCTAA10250.010.019431
Produced by FastQC (version 0.11.5)