FastQCFastQC Report
Thu 26 May 2016
SRR1512934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008955
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33920.16884400098558702No Hit
GTCCTAAAGTGTGTATTTCTCATTT31990.1592370162596972No Hit
CTGTAGGACGTGGAATATGGCAAGA29910.1488833746898263No Hit
GTACATGGGAAGCAGTGGTATCAAC28060.13967460694739303No Hit
CTTTAGGACGTGAAATATGGCGAGG26590.1323573698763785No Hit
CCCATGTACTCTGCGTTGATACCAC23930.11911665517644746No Hit
GTATCAACGCAGAGTACTTTTTTTT21850.10876301360657654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.014.5528274
ACCGACC400.005296411514.2432598
GGCGAGG7650.011.791512519
GGTTAGC752.0212567E-411.431891
GTCTTAG2100.010.8875141
AGGACCT13500.010.763455
TGGCGAG17400.010.70328218
TGCCCGA909.560678E-510.55082610
GTAGGAC18500.010.52440553
GGACCTG13300.010.4233796
GTCCTAA10350.010.4009941
GCTAGAC1106.0648945E-610.3610553
ACGTGAA9950.010.3066818
GGACGTG17300.010.1539026
AGGACGT17900.010.1338425
TGTAGGA19450.010.1080232
ATGGCGA19550.010.06082717
TTAGGAC12000.010.0516623
TATGGCG19400.010.0406616
GGCGAGA10250.010.00477219
Produced by FastQC (version 0.11.5)