##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512934_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2008955 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.143881271606382 31.0 28.0 33.0 16.0 34.0 2 28.261625571503593 31.0 28.0 34.0 16.0 34.0 3 28.48666147325351 31.0 28.0 34.0 16.0 34.0 4 31.83129089501756 35.0 32.0 37.0 17.0 37.0 5 31.95607517341105 35.0 33.0 37.0 19.0 37.0 6 31.976964640820725 35.0 33.0 37.0 18.0 37.0 7 31.75946101331289 35.0 32.0 37.0 17.0 37.0 8 31.760917989701113 35.0 32.0 37.0 17.0 37.0 9 32.987680659845545 38.0 33.0 39.0 17.0 39.0 10 32.849717390384555 37.0 33.0 39.0 16.0 39.0 11 32.89835411943025 37.0 33.0 39.0 16.0 39.0 12 32.78343268017452 37.0 33.0 39.0 11.0 39.0 13 32.77410494510828 37.0 33.0 39.0 11.0 39.0 14 33.67402853722458 39.0 33.0 41.0 10.0 41.0 15 33.5959103115799 39.0 33.0 41.0 10.0 41.0 16 33.4866002473923 38.0 33.0 41.0 10.0 41.0 17 33.410070409740385 38.0 33.0 41.0 10.0 41.0 18 33.11678459696708 38.0 32.0 41.0 8.0 41.0 19 33.328353795878954 39.0 32.0 41.0 2.0 41.0 20 33.39302124736492 39.0 33.0 41.0 2.0 41.0 21 33.31641574848615 39.0 33.0 41.0 2.0 41.0 22 33.21948177037316 39.0 33.0 41.0 2.0 41.0 23 32.947047096624864 39.0 32.0 41.0 2.0 41.0 24 32.92114109076609 39.0 32.0 41.0 2.0 41.0 25 32.82050469024941 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4363599922978132 1101 2 0.7227576565130356 1101 3 0.8524441417897499 1101 4 0.6792724958504905 1101 5 0.5230560859179398 1101 6 0.827627519645457 1101 7 0.6788517768401903 1101 8 0.48349238303419995 1101 9 0.4084793180302668 1101 10 0.6282223946619965 1101 11 0.7898856441470983 1101 12 0.9981035171042336 1101 13 0.8077720558873338 1101 14 0.6836592935439754 1101 15 0.3611004332382919 1101 16 0.9314808030416799 1101 17 0.8472876503322198 1101 18 1.2328707457199783 1101 19 0.6184096617728585 1101 20 0.7648127526881865 1101 21 0.9003751344038662 1101 22 0.8291343435172394 1101 23 1.016695056997122 1101 24 0.7208007717986149 1101 25 1.0531523880801075 1102 1 0.5126227994385708 1102 2 0.6014210200866081 1102 3 0.6495661252269969 1102 4 0.9200135057551222 1102 5 0.6777114433085849 1102 6 0.653082092231724 1102 7 0.8096434300378945 1102 8 0.7446601865199263 1102 9 0.730328666442638 1102 10 0.8233853128877655 1102 11 0.8280312918703956 1102 12 0.7374657511455496 1102 13 0.721976712775728 1102 14 0.8092217922358387 1102 15 1.0117790556911146 1102 16 0.9788598595244977 1102 17 0.7825275296193226 1102 18 1.0955641851926003 1102 19 1.012210587042027 1102 20 1.0538437665695497 1102 21 0.8241604860658427 1102 22 1.1328519511507125 1102 23 1.3528393334057824 1102 24 1.2400495642432858 1102 25 1.074132668746124 1103 1 0.3466996368971387 1103 2 0.5226518456249423 1103 3 0.5595536604288682 1103 4 0.748948324734009 1103 5 0.24095072115248328 1103 6 0.7466075127836795 1103 7 0.5539549196352667 1103 8 0.40833567444812857 1103 9 0.8315074807595693 1103 10 0.7522142852129861 1103 11 0.8064377260510938 1103 12 1.0185149385039622 1103 13 0.9448337663782169 1103 14 1.2113858539705262 1103 15 1.2100091662004928 1103 16 1.2491545910646806 1103 17 0.9543332901147039 1103 18 1.342927082054885 1103 19 1.4031514131897111 1103 20 1.224927845335344 1103 21 1.1015285663095256 1103 22 1.2098694203801728 1103 23 1.614641649883609 1103 24 1.40519386973574 1103 25 1.3599362779839552 1104 1 0.542440473246959 1104 2 0.47010566137331367 1104 3 0.5168113996282209 1104 4 0.747262849390026 1104 5 0.6113490240227115 1104 6 0.5052307778922334 1104 7 0.8430281971566487 1104 8 0.8848429878386348 1104 9 0.8334835486800856 1104 10 1.0117467681254269 1104 11 1.051296579851062 1104 12 0.9112549817002105 1104 13 0.8847740312197061 1104 14 1.0292577486283676 1104 15 1.1620531350557286 1104 16 1.0310290063035197 1104 17 1.0106318338110114 1104 18 1.2701988737621193 1104 19 1.1276172345299074 1104 20 1.0854203685525121 1104 21 1.1945785749207403 1104 22 1.0372588699208407 1104 23 1.3709257743513135 1104 24 1.1762637515383858 1104 25 1.0445278442136257 1105 1 0.7935223335698396 1105 2 0.8287502116888064 1105 3 0.8773674194419527 1105 4 1.0748046250564371 1105 5 0.8518785357038503 1105 6 0.7051281743121969 1105 7 0.9227823270359039 1105 8 1.0573456825545726 1105 9 0.8349028162017973 1105 10 1.138611496413283 1105 11 1.0272023374598476 1105 12 1.0555063048360083 1105 13 1.0434382998919673 1105 14 1.0458083002881935 1105 15 1.3279007897445325 1105 16 1.188264356434921 1105 17 1.0748084618433253 1105 18 1.0669552229973007 1105 19 1.1052049187697506 1105 20 1.0545834700191676 1105 21 1.0515885075225597 1105 22 0.8074576960541364 1105 23 0.9067730657076325 1105 24 1.0729115551935138 1105 25 1.1841112910883567 1106 1 0.7530223470121484 1106 2 0.7801545965689023 1106 3 0.9233132200656762 1106 4 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1107 17 1.1773469613292065 1107 18 1.4034720819385171 1107 19 1.3154086198318709 1107 20 1.1479713764851738 1107 21 1.1563397801898603 1107 22 1.184106015657818 1107 23 1.2831059116680876 1107 24 1.1879475286614039 1107 25 1.2344294764861914 1108 1 0.4430641188921385 1108 2 0.5297735238124446 1108 3 0.5457916726530705 1108 4 0.6473379784129136 1108 5 0.6926584369230362 1108 6 0.8346868680620112 1108 7 0.8102721300675526 1108 8 0.8901294919936227 1108 9 0.9466730840668802 1108 10 0.9335877282070584 1108 11 0.8203280975974891 1108 12 0.8744463392461412 1108 13 0.9331968356814713 1108 14 1.0128654181429013 1108 15 1.078681692120611 1108 16 1.0595345835121392 1108 17 1.0790207367684275 1108 18 1.208160615895764 1108 19 1.2393907186570274 1108 20 1.108757274563672 1108 21 0.9496092759660968 1108 22 0.9396822481291238 1108 23 1.159467290144761 1108 24 1.2745078442386273 1108 25 1.3758466482418115 1109 1 0.33204189185972055 1109 2 0.18904137519931297 1109 3 0.5163010333229643 1109 4 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-4.01681230575576 2216 25 -4.354326211450992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 130067.0 3 4.0 4 1048.0 5 6034.0 6 7179.0 7 6492.0 8 5948.0 9 5405.0 10 5233.0 11 4813.0 12 4667.0 13 4566.0 14 4570.0 15 4623.0 16 4816.0 17 5059.0 18 5691.0 19 6760.0 20 8526.0 21 11429.0 22 13909.0 23 16822.0 24 19244.0 25 21899.0 26 24455.0 27 26853.0 28 29944.0 29 35532.0 30 43334.0 31 53484.0 32 65372.0 33 82149.0 34 104285.0 35 133891.0 36 178156.0 37 268522.0 38 658174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75344036811814 18.23412585428524 12.29975933657215 25.712674441024475 2 14.823875997866704 20.040395207749985 41.08554153331484 24.05018726106847 3 18.658361513192457 24.57162547535617 29.528873919384775 27.241139092066604 4 12.303159408512197 16.662135299647847 38.366984957853305 32.66772033398665 5 13.280382441550682 37.76983791051465 35.91210815925105 13.037671488683614 6 32.056867668064164 36.82015221600394 18.21942026084926 12.903559855082639 7 28.052524146171464 31.285970327591357 22.43179328885791 18.22971223737927 8 25.15947414855841 36.62707445844511 20.798406353872288 17.41504503912419 9 26.600794410251456 14.964197768123416 19.905065087272607 38.52994273435252 10 14.949869208870497 28.62857011987776 34.75792936781134 21.6636313034404 11 34.337347298153766 22.130061620923534 24.788966882425367 18.74362419849734 12 23.374163762832012 24.505178110986478 31.697626796631006 20.423031329550508 13 29.127216585488743 21.791181802204672 25.57211641626227 23.509485196044313 14 21.039777613024555 21.91530434063885 27.487526084313263 29.55739196202333 15 23.35509036329045 30.506529349584472 23.047481244955545 23.090899042169536 16 22.436492016288554 27.670178195264487 27.581755186761242 22.311574601685713 17 22.062185710729402 28.309036594473486 27.58790141896938 22.04087627582773 18 22.112996528924715 27.18369596031007 29.209743722345866 21.493563788419355 19 23.78645707303812 26.80563895286832 27.140970834341573 22.266933139751988 20 23.883983360758375 27.194057271453147 27.509217054475844 21.412742313312638 21 23.237955541481572 26.667343811148385 26.80819982135335 23.286500826016688 22 23.36836518628087 27.900135072363042 26.66252770028971 22.068972041066377 23 22.66272872836192 27.563908516621392 27.14334507014204 22.630017684874655 24 23.32314037407596 26.847378441243947 27.7466722693092 22.08280891537089 25 23.001895452854903 26.992453538502286 27.36487243408111 22.640778574561697 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 192.0 2 324.5 3 457.0 4 457.0 5 457.0 6 900.0 7 1343.0 8 1343.0 9 1343.0 10 1950.5 11 2558.0 12 2558.0 13 2558.0 14 4812.0 15 7066.0 16 7066.0 17 7066.0 18 13736.0 19 20406.0 20 20406.0 21 20406.0 22 33402.0 23 46398.0 24 46398.0 25 46398.0 26 67686.5 27 88975.0 28 88975.0 29 88975.0 30 115881.0 31 142787.0 32 142787.0 33 142787.0 34 164412.5 35 186038.0 36 186038.0 37 186038.0 38 203081.5 39 220125.0 40 220125.0 41 220125.0 42 233995.5 43 247866.0 44 247866.0 45 247866.0 46 258245.5 47 268625.0 48 268625.0 49 268625.0 50 259548.0 51 250471.0 52 250471.0 53 250471.0 54 224471.5 55 198472.0 56 198472.0 57 198472.0 58 171672.0 59 144872.0 60 144872.0 61 144872.0 62 118248.5 63 91625.0 64 91625.0 65 91625.0 66 71418.0 67 51211.0 68 51211.0 69 51211.0 70 37678.0 71 24145.0 72 24145.0 73 24145.0 74 16988.5 75 9832.0 76 9832.0 77 9832.0 78 6805.5 79 3779.0 80 3779.0 81 3779.0 82 2492.0 83 1205.0 84 1205.0 85 1205.0 86 785.5 87 366.0 88 366.0 89 366.0 90 237.0 91 108.0 92 108.0 93 108.0 94 64.0 95 20.0 96 20.0 97 20.0 98 16.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34789231217224875 2 0.03962258985392903 3 0.036038637002819875 4 0.03638707686334437 5 0.04007058396031767 6 0.018019318501409937 7 0.017670878640885436 8 0.02384324188446232 9 0.020956168754402163 10 0.020358843279217304 11 0.019114415205915513 12 0.020109957664556945 13 0.020109957664556945 14 0.02807429733368841 15 0.027974743087824265 16 0.021304608614926668 17 0.0226485909340926 18 0.08939971278600069 19 0.024739230097239606 20 0.022399705319432243 21 0.025933881047609332 22 0.020010403418692802 23 0.022847699425820885 24 0.018865529591255155 25 0.023096585040481246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2008955.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.80410833769967 #Duplication Level Percentage of deduplicated Percentage of total 1 83.12308031064073 59.68578663988558 2 11.354651647491353 16.306212740666187 3 2.9828312958534116 6.425386245616184 4 1.0643624225374795 3.057023787938306 5 0.5010854519952447 1.7989997040755883 6 0.2755550938134589 1.1871592685511962 7 0.1509339720996631 0.7586375499138485 8 0.10609058642714894 0.6094191969140075 9 0.06901698126796965 0.44601325200956543 >10 0.3357107793809971 4.179335733023409 >50 0.01937200072831294 0.9402600904219566 >100 0.013835418987029148 1.9652350434379344 >500 0.0023623463684814953 1.0927417946134927 >1k 0.0011116924086970975 1.5477889529328004 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3392 0.16884400098558702 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3199 0.1592370162596972 No Hit CTGTAGGACGTGGAATATGGCAAGA 2991 0.1488833746898263 No Hit GTACATGGGAAGCAGTGGTATCAAC 2806 0.13967460694739303 No Hit CTTTAGGACGTGAAATATGGCGAGG 2659 0.1323573698763785 No Hit CCCATGTACTCTGCGTTGATACCAC 2393 0.11911665517644746 No Hit GTATCAACGCAGAGTACTTTTTTTT 2185 0.10876301360657654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 620 0.0 14.552827 4 ACCGACC 40 0.0052964115 14.243259 8 GGCGAGG 765 0.0 11.7915125 19 GGTTAGC 75 2.0212567E-4 11.43189 1 GTCTTAG 210 0.0 10.887514 1 AGGACCT 1350 0.0 10.76345 5 TGGCGAG 1740 0.0 10.703282 18 TGCCCGA 90 9.560678E-5 10.550826 10 GTAGGAC 1850 0.0 10.5244055 3 GGACCTG 1330 0.0 10.423379 6 GTCCTAA 1035 0.0 10.400994 1 GCTAGAC 110 6.0648945E-6 10.361055 3 ACGTGAA 995 0.0 10.306681 8 GGACGTG 1730 0.0 10.153902 6 AGGACGT 1790 0.0 10.133842 5 TGTAGGA 1945 0.0 10.108023 2 ATGGCGA 1955 0.0 10.060827 17 TTAGGAC 1200 0.0 10.051662 3 TATGGCG 1940 0.0 10.04066 16 GGCGAGA 1025 0.0 10.004772 19 >>END_MODULE