FastQCFastQC Report
Thu 26 May 2016
SRR1512934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008955
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT31220.1554041777939277No Hit
CTGTAGGACGTGGAATATGGCAAGA30740.15301487589318824No Hit
GTCCTAAAGTGTGTATTTCTCATTT29910.1488833746898263No Hit
CTTTAGGACGTGAAATATGGCGAGG29590.14729050675600003No Hit
GTACATGGGAAGCAGTGGTATCAAC28640.1425616800774532No Hit
CCCATGTACTCTGCGTTGATACCAC27530.13703641943199327No Hit
GTATCAACGCAGAGTACTTTTTTTT27350.13614043121921596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7500.012.177824
TAACCTC1552.5465852E-1111.6622275
CTAGGAC3450.011.5732153
AGGACCT14450.010.60028655
TGGCGAG18100.010.53967618
TAGAAAT6000.010.4653154
GGCGAGG7450.010.44667719
GTATTAA2200.010.3710611
GGACCTG13700.010.2744816
GTCCTAC17900.010.1972451
TCCAACG1606.2700565E-910.08413718
GTACCGT856.52596E-410.0702986
GAAATGT6050.010.061056
ATTTAGA5500.010.0253591
ACGGAGG951.639931E-410.0026768
CTACACT4000.09.9896184
TATGGCG19950.09.9451316
GCCCTAA1151.017915E-59.9201451
TCTAGGA4050.09.8579272
AGGACGT15750.09.8461545
Produced by FastQC (version 0.11.5)