##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512934_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2008955
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.601638662886923	31.0	31.0	34.0	27.0	34.0
2	30.719330696805056	31.0	31.0	34.0	27.0	34.0
3	30.831089297669685	31.0	31.0	34.0	27.0	34.0
4	34.341910097538275	37.0	35.0	37.0	31.0	37.0
5	34.0393946106309	37.0	35.0	37.0	30.0	37.0
6	33.923934085133816	37.0	35.0	37.0	30.0	37.0
7	33.80130416062082	37.0	35.0	37.0	29.0	37.0
8	33.68309842679403	37.0	35.0	37.0	28.0	37.0
9	35.15150613129712	39.0	35.0	39.0	28.0	39.0
10	35.044273764220705	39.0	35.0	39.0	28.0	39.0
11	35.093455054991274	39.0	35.0	39.0	28.0	39.0
12	34.94740449636751	39.0	35.0	39.0	27.0	39.0
13	34.95217214920195	39.0	35.0	39.0	27.0	39.0
14	36.01130438461787	40.0	36.0	41.0	27.0	41.0
15	36.02845758118026	40.0	36.0	41.0	27.0	41.0
16	36.004232548762914	40.0	36.0	41.0	27.0	41.0
17	35.981397293617825	40.0	36.0	41.0	27.0	41.0
18	35.89443914871164	40.0	36.0	41.0	27.0	41.0
19	35.87621474846375	40.0	36.0	41.0	27.0	41.0
20	35.86128310489782	40.0	36.0	41.0	27.0	41.0
21	35.80432961415263	40.0	36.0	41.0	27.0	41.0
22	35.775207010609996	40.0	36.0	41.0	27.0	41.0
23	35.66916282345797	40.0	36.0	41.0	27.0	41.0
24	35.6523496046452	40.0	36.0	41.0	27.0	41.0
25	35.55309949700217	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5909086985804528
1101	2	0.569639517160553
1101	3	0.5541999259146344
1101	4	0.32648567951427054
1101	5	0.6675812210981604
1101	6	0.6121220088224391
1101	7	0.6289407834219531
1101	8	0.566061397694547
1101	9	0.431616455412815
1101	10	0.7853308992564578
1101	11	0.9160566515325499
1101	12	0.7916323766066782
1101	13	0.8750007329554137
1101	14	0.9949624488944195
1101	15	0.9813581506151934
1101	16	0.9942992049028518
1101	17	0.9298701439597821
1101	18	0.381463073460111
1101	19	0.747169339910748
1101	20	0.7737940118994473
1101	21	1.016403997330798
1101	22	0.896108752745107
1101	23	0.8136100601551277
1101	24	0.8590504186023296
1101	25	0.9609915368083861
1102	1	0.5013146239930286
1102	2	0.46789595532387196
1102	3	0.49761769316013726
1102	4	0.3848910669436023
1102	5	0.5761820150498025
1102	6	0.555204021034811
1102	7	0.6360426079913566
1102	8	0.6436624769168446
1102	9	0.8405062021808476
1102	10	0.8150209464889073
1102	11	0.7456572416086971
1102	12	0.8037576391060668
1102	13	0.853806693096594
1102	14	1.0110643494326297
1102	15	0.919970115083494
1102	16	0.8923742481391841
1102	17	0.9316765351077123
1102	18	0.8279123259037107
1102	19	0.9990198201034346
1102	20	0.9080195718400077
1102	21	0.8797135206419853
1102	22	0.9211758456123391
1102	23	0.9558606983319393
1102	24	0.9563663744566995
1102	25	0.9872353711250526
1103	1	0.6435418466184899
1103	2	0.5111413228695803
1103	3	0.5604609861191463
1103	4	0.5645252181764704
1103	5	0.6045095415047186
1103	6	0.7445427426679458
1103	7	0.6910069876709599
1103	8	0.6284334979581345
1103	9	0.7651998465993444
1103	10	0.796298873691704
1103	11	0.8235604944119288
1103	12	0.7714065321224624
1103	13	0.9015002619241059
1103	14	0.9448023001920518
1103	15	0.8159104552891776
1103	16	0.880830040143664
1103	17	1.0352367545066699
1103	18	1.0591351989625437
1103	19	1.0921904258571402
1103	20	1.1998984203271448
1103	21	0.7533308999336015
1103	22	1.0848352318907217
1103	23	1.1799364636327425
1103	24	1.144057328453414
1103	25	1.233845829214097
1104	1	0.44631463931970927
1104	2	0.5592360742895686
1104	3	0.5216035650260231
1104	4	0.4121405638453126
1104	5	0.5495423284497676
1104	6	0.6422314036822527
1104	7	0.6648749678250283
1104	8	0.5624343270157794
1104	9	0.6803830063227281
1104	10	0.7308510318431871
1104	11	0.7656604755382901
1104	12	0.6821229949839278
1104	13	0.834084512171259
1104	14	0.9021483595666311
1104	15	0.9179688337730028
1104	16	0.8964741351815491
1104	17	0.7283700336830918
1104	18	0.8375825034019968
1104	19	0.9019044163296996
1104	20	1.001302922313677
1104	21	0.7457251888438705
1104	22	0.8649730996842493
1104	23	0.7524315834630642
1104	24	0.716217023618249
1104	25	1.0175961841747991
1105	1	0.44153700637030013
1105	2	0.4879933863558392
1105	3	0.4282604217155779
1105	4	0.41555797080192036
1105	5	0.35479781931195475
1105	6	0.45510306253726895
1105	7	0.5876000144526969
1105	8	0.7761758265495047
1105	9	0.6926780262454599
1105	10	0.6532630826734049
1105	11	0.6353372761535354
1105	12	0.6131475396072119
1105	13	0.6892755115172591
1105	14	0.8379463739546011
1105	15	0.7647441444222736
1105	16	0.8591332066853852
1105	17	0.6433305515529995
1105	18	0.9024589249403476
1105	19	0.9736699624184268
1105	20	0.8800096706090415
1105	21	0.6715953322996455
1105	22	0.7794264659597303
1105	23	0.7928674613349074
1105	24	0.9147840932811988
1105	25	0.9455322134351789
1106	1	0.43260179380263963
1106	2	0.44952822881201726
1106	3	0.5086006016632432
1106	4	0.43563461434782624
1106	5	0.531630789703037
1106	6	0.503190670523793
1106	7	0.6267893762120309
1106	8	0.6318889184590262
1106	9	0.6205667777241146
1106	10	0.7020356881506942
1106	11	0.6135663420029047
1106	12	0.7477492664954042
1106	13	0.55063997274965
1106	14	0.7674224376890493
1106	15	0.7107368810964729
1106	16	0.9019497505789928
1106	17	0.8085857761291138
1106	18	0.9506619127168108
1106	19	0.8690759424521204
1106	20	0.9631032828254149
1106	21	0.9157641721390561
1106	22	0.9259834434125054
1106	23	1.0156064655289043
1106	24	0.9345806887932611
1106	25	0.9949371673728749
1107	1	0.42497663676929065
1107	2	0.39241740967841565
1107	3	0.4193465500135183
1107	4	0.39055382887537604
1107	5	0.4515241557240586
1107	6	0.5090558349163032
1107	7	0.4789590455613535
1107	8	0.5280158263414805
1107	9	0.34241331441953804
1107	10	0.5916704989136221
1107	11	0.545245544553346
1107	12	0.48615114958408867
1107	13	0.5600265078084732
1107	14	0.7760743494552003
1107	15	0.7041901998223068
1107	16	0.5338382755119468
1107	17	0.5909892300592574
1107	18	0.6879002574296962
1107	19	0.7436724029975679
1107	20	0.8321494307264885
1107	21	0.6131377968908183
1107	22	0.6901569903610607
1107	23	0.657203950704826
1107	24	0.6909121274745686
1107	25	0.6726405469171013
1108	1	0.440405050183891
1108	2	0.40587961043700105
1108	3	0.3593200165065973
1108	4	0.4857975624782327
1108	5	0.49757808762200284
1108	6	0.4804983341103437
1108	7	0.4482555682778582
1108	8	0.5289151957464995
1108	9	0.45140329396222967
1108	10	0.5848303937884722
1108	11	0.40860101054290254
1108	12	0.6040582401203949
1108	13	0.576095559127836
1108	14	0.6182986699410762
1108	15	0.5870785641962328
1108	16	0.6152683517418964
1108	17	0.6599060017028009
1108	18	0.5558845501072085
1108	19	0.6043746100111989
1108	20	0.6022527811664276
1108	21	0.7413692006215626
1108	22	0.7360074039324971
1108	23	0.7354158876645869
1108	24	0.7981236374952516
1108	25	0.7422380364603001
1109	1	0.09095713795325722
1109	2	0.17036576765672606
1109	3	0.2368979358192007
1109	4	0.23692161929398026
1109	5	0.15357223382115137
1109	6	-0.033601843997665526
1109	7	0.13234937026034288
1109	8	0.33155740569748815
1109	9	0.3337146041736787
1109	10	0.2817145624099311
1109	11	0.3245744990364656
1109	12	0.3428973147116494
1109	13	0.35053506004896207
1109	14	0.44676818806127017
1109	15	0.4447050926429341
1109	16	0.2493065096768774
1109	17	0.33009373271971043
1109	18	0.3471123189573646
1109	19	0.5754947611559587
1109	20	0.4285158304602348
1109	21	0.5838167858110666
1109	22	0.47102833221167373
1109	23	0.46657678954519355
1109	24	0.007653474704063967
1109	25	0.15280358232487856
1110	1	0.21854299319125658
1110	2	0.2686842145997055
1110	3	0.23936974704862735
1110	4	0.4238010107319212
1110	5	0.29831421721902274
1110	6	0.36788566191582106
1110	7	0.29839716345802003
1110	8	0.4447906654723184
1110	9	0.5170713081483882
1110	10	0.6314414327930535
1110	11	0.4462803009044194
1110	12	0.5274173572792691
1110	13	0.23379998114117484
1110	14	0.5036721588040578
1110	15	0.5707288447687162
1110	16	0.5485300378222107
1110	17	0.44426540636924017
1110	18	0.30531371439711563
1110	19	0.48344653926045567
1110	20	0.653088619857229
1110	21	0.5204933956233404
1110	22	0.5043809286191134
1110	23	0.4839367852393295
1110	24	0.4724507733412011
1110	25	0.4880069446755826
1111	1	-0.23235439753639753
1111	2	-0.05719792025530168
1111	3	-0.024008981620976755
1111	4	0.0033660475724559547
1111	5	0.25961975914983526
1111	6	0.10564469511950136
1111	7	0.24260864401071558
1111	8	0.2786860673714955
1111	9	-0.20665980623266478
1111	10	-0.06125386348558948
1111	11	0.19291594921567423
1111	12	0.1435891963584055
1111	13	0.24146303475664155
1111	14	0.40379052134807125
1111	15	0.5361162441471237
1111	16	0.2202023577971559
1111	17	0.46478081307353136
1111	18	0.36947991475220476
1111	19	0.4184613192266369
1111	20	0.3804078387258514
1111	21	0.3885462166356817
1111	22	0.23960345711017084
1111	23	0.37432305242089825
1111	24	0.4660335200430552
1111	25	0.47322736229118334
1112	1	0.061600560861862164
1112	2	-0.12347150192505296
1112	3	-0.0051139512894380346
1112	4	-0.27237950069830674
1112	5	-0.003845876538477455
1112	6	0.014601466550033138
1112	7	-0.1098187611970971
1112	8	0.09690684570134778
1112	9	-0.15576132623971972
1112	10	0.1304242203842989
1112	11	-0.05614186201336224
1112	12	0.16726034170478954
1112	13	0.07093049327740886
1112	14	0.0647150698050325
1112	15	-0.024492346685796917
1112	16	-0.23873814534380244
1112	17	-0.07510766094130616
1112	18	-0.04734100666019714
1112	19	0.050548952386506585
1112	20	0.2296596934088626
1112	21	0.31269570849664063
1112	22	0.18485282254887636
1112	23	0.32003116402528065
1112	24	0.22503517627253444
1112	25	0.26925770363125423
1113	1	-0.116583291843952
1113	2	-0.566164435367341
1113	3	-0.1645788982479246
1113	4	-0.17657055320977122
1113	5	-0.02769681553153447
1113	6	-0.061506810281755975
1113	7	0.15240522784859678
1113	8	0.26305126879667995
1113	9	0.20821137633763698
1113	10	-0.015118746554016127
1113	11	0.0942286555922962
1113	12	-0.03361305803165493
1113	13	0.07489671750202831
1113	14	0.11314340670882217
1113	15	0.1925068508818768
1113	16	-0.09324288263923108
1113	17	0.05201116529897121
1113	18	0.20745643439047257
1113	19	-0.02623955148210655
1113	20	0.14486335662286365
1113	21	-0.13006524764933403
1113	22	-0.12829749731978524
1113	23	0.11976260685749907
1113	24	-0.2641773357823496
1113	25	-0.1172063672772623
1114	1	-0.5839679145903318
1114	2	-0.3749702099353911
1114	3	-0.3439124009018393
1114	4	-0.11824513377324308
1114	5	-0.34645569565992673
1114	6	-0.6880987918478993
1114	7	-0.4903872366492905
1114	8	-0.9823954798800472
1114	9	-0.701084323655742
1114	10	-0.7681416710885642
1114	11	-0.8070979343647835
1114	12	-0.8483468934373235
1114	13	-0.5647025816579969
1114	14	-1.8067707183086625
1114	15	-0.9598411838951009
1114	16	-1.048130910201678
1114	17	-1.0468389270911729
1114	18	-1.2478061229392665
1114	19	-1.0782494972258974
1114	20	-0.40950051330948156
1114	21	-0.49142573853178817
1114	22	-0.5674727588464705
1114	23	-0.5055631512201941
1114	24	-1.2550294232106651
1114	25	-1.1858327356452278
1115	1	-1.698823486986118
1115	2	-1.5400863984662898
1115	3	-1.1707303308573493
1115	4	-2.4069432572646363
1115	5	-1.5789400460163776
1115	6	-1.3663102960787796
1115	7	-1.1333761782317993
1115	8	-1.1453505056021385
1115	9	-1.5241650354361127
1115	10	-1.295335246812563
1115	11	-1.3315044973463372
1115	12	-1.855177145732938
1115	13	-1.3802418583248581
1115	14	-2.032636450976355
1115	15	-2.1270402191129065
1115	16	-1.6276455403775643
1115	17	-1.557727131506212
1115	18	-1.4180445479975319
1115	19	-1.2340345235455459
1115	20	-1.2521328675554884
1115	21	-1.401644453278216
1115	22	-1.6907907730031297
1115	23	-2.152901806428261
1115	24	-1.4837344548177853
1115	25	-1.341127720335109
1116	1	-1.6417129044496868
1116	2	-1.6057882186492165
1116	3	-1.3009400549564951
1116	4	-1.6589510736195265
1116	5	-1.4875788329683104
1116	6	-2.337054584207955
1116	7	-1.5989401978937323
1116	8	-1.9071426352814527
1116	9	-2.221033449948372
1116	10	-2.141767802031538
1116	11	-1.9916778491688873
1116	12	-2.726096052171762
1116	13	-2.1774355246565023
1116	14	-2.8566059521737728
1116	15	-2.7375626534468154
1116	16	-3.058249472595847
1116	17	-2.7982153790943514
1116	18	-3.695752525773486
1116	19	-2.998656915653875
1116	20	-2.6518486644882913
1116	21	-2.6671648744019834
1116	22	-4.169194085819559
1116	23	-4.198945617355189
1116	24	-3.283669000971294
1116	25	-3.7910221844135243
1201	1	0.734530326699371
1201	2	0.6960557975856183
1201	3	0.5518147352566132
1201	4	0.6546983176744448
1201	5	0.712791693013834
1201	6	0.7475882598146768
1201	7	0.7179184459174763
1201	8	0.8471876802561766
1201	9	0.9655288646052682
1201	10	0.8544864400784533
1201	11	1.0956333536626133
1201	12	0.8240249467789198
1201	13	0.8199876446772052
1201	14	0.839905659550304
1201	15	1.0094026557571922
1201	16	1.0251153627180685
1201	17	0.9018132955442937
1201	18	0.9816344686291316
1201	19	0.9004463467727319
1201	20	0.9979760716567654
1201	21	0.8747387780598928
1201	22	0.9620856067562187
1201	23	0.953746770725644
1201	24	0.9548013054812259
1201	25	0.9222398296895804
1202	1	0.7279432516350184
1202	2	0.770597486728601
1202	3	0.7976470326196008
1202	4	0.6848374696957578
1202	5	0.8233259241396667
1202	6	0.8773444784235593
1202	7	0.8621841622540174
1202	8	0.7480964746387215
1202	9	0.950370013395812
1202	10	0.9241186718178724
1202	11	0.9967165303281575
1202	12	0.9367586822868503
1202	13	0.903410035110312
1202	14	0.9814690805642883
1202	15	1.0863361641513407
1202	16	0.9915358682909883
1202	17	1.1447617422673062
1202	18	1.0744001241402756
1202	19	1.2868083727284514
1202	20	1.3532076128462194
1202	21	1.2800359670543244
1202	22	1.16783450305401
1202	23	1.2043760833308426
1202	24	1.0525103449101394
1202	25	1.123786297139759
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2211	11	-0.102934740896238
2211	12	0.024051611105655013
2211	13	-0.03163713499470333
2211	14	-1.3341260070585435
2211	15	-0.5261638538095568
2211	16	-0.24258466321745686
2211	17	-0.01646015431720116
2211	18	-0.2985444427841841
2211	19	-0.284666465970723
2211	20	-0.3389998963413774
2211	21	-0.4217731023844209
2211	22	-0.2585344884871219
2211	23	-0.4642355968080949
2211	24	-0.43428418099163224
2211	25	-0.4200884382962329
2212	1	-0.210692820175705
2212	2	-0.1170767493907654
2212	3	-0.15501257979271443
2212	4	0.022387344859239988
2212	5	-0.11561451100478592
2212	6	-0.10772650005699802
2212	7	-0.18964225553222036
2212	8	-0.39247896228911117
2212	9	-0.3561191079928463
2212	10	-0.4220361254713936
2212	11	-0.23952886260966721
2212	12	-0.16371878769294312
2212	13	-0.28193772377685633
2212	14	-0.30813168034404015
2212	15	-0.2228537062564584
2212	16	-0.1454024267987748
2212	17	-0.36735572295680896
2212	18	-0.04285442347594426
2212	19	0.04724066377588798
2212	20	-0.2145883686066412
2212	21	-0.1050383404001991
2212	22	-0.1667273801941178
2212	23	-0.5891566713951093
2212	24	-0.41444007715620756
2212	25	-0.48397306559951403
2213	1	-0.323719425149676
2213	2	-0.41834414865816427
2213	3	-0.2846800176180473
2213	4	-0.36500787395703327
2213	5	-0.22680093861226425
2213	6	-0.05094146053512105
2213	7	-0.6359206438106071
2213	8	-0.2074712507024543
2213	9	-0.28016769098879735
2213	10	-0.42170144261039155
2213	11	-0.32054968389364547
2213	12	-0.40503901132895237
2213	13	-0.23835376387710738
2213	14	-0.1301494169933548
2213	15	-0.341769842751539
2213	16	-0.5700947344910858
2213	17	-0.5075060988966555
2213	18	-0.5865162704009492
2213	19	-0.8796789429331895
2213	20	-0.2900570402858378
2213	21	-1.057555209496016
2213	22	-0.4755860653685886
2213	23	-0.5906280421296373
2213	24	-0.7593668170829488
2213	25	-0.8196896647897987
2214	1	-1.0496171995552146
2214	2	-0.9302166592607826
2214	3	-0.7018780881414628
2214	4	-1.2337867675167047
2214	5	-1.121455455025206
2214	6	-2.5531205452261894
2214	7	-1.1512279180918057
2214	8	-1.8389813457843829
2214	9	-1.1927097980342651
2214	10	-1.4486350991357213
2214	11	-1.2108711742093448
2214	12	-1.2122513360791984
2214	13	-1.5128622073528817
2214	14	-1.5559482944782985
2214	15	-1.8515910007939524
2214	16	-1.2302284967667347
2214	17	-1.4498955130008326
2214	18	-1.5498285045929308
2214	19	-3.3586413336712937
2214	20	-1.8848775660329906
2214	21	-2.305050322999385
2214	22	-2.04566665967522
2214	23	-1.9912719648385462
2214	24	-1.2823116028536674
2214	25	-1.0518665767615616
2215	1	-1.2782327175406962
2215	2	-1.1173178618904345
2215	3	-1.0939958018938079
2215	4	-0.9006605426145029
2215	5	-1.6636128092844515
2215	6	-1.5126453226792478
2215	7	-1.7371460976976536
2215	8	-2.512933640690555
2215	9	-1.8955187197878942
2215	10	-1.2088676817478188
2215	11	-1.8497460978459088
2215	12	-2.576832563650825
2215	13	-1.9530472986126668
2215	14	-1.9442770710749855
2215	15	-3.268047037639903
2215	16	-2.5966674963300065
2215	17	-2.234600797274126
2215	18	-2.9066016116489877
2215	19	-3.7991345123787568
2215	20	-3.1043512445140067
2215	21	-2.163031373982591
2215	22	-3.2380294621425847
2215	23	-3.168186265375276
2215	24	-2.0592676450255993
2215	25	-2.2961346226736197
2216	1	-1.3345165952402596
2216	2	-1.0829535066989386
2216	3	-1.426523306943725
2216	4	-1.0043596984000303
2216	5	-0.8703783861681629
2216	6	-1.3816106532018253
2216	7	-2.833594947680517
2216	8	-1.8110890550558558
2216	9	-2.062596203214248
2216	10	-1.9132748643537312
2216	11	-2.3403309283433487
2216	12	-1.8138267286537655
2216	13	-2.3786306371626864
2216	14	-2.2409530007559155
2216	15	-2.3158536743778484
2216	16	-2.1771709881172754
2216	17	-2.4102649645647674
2216	18	-2.138018056361915
2216	19	-2.4987660307314243
2216	20	-2.822895040798933
2216	21	-2.6474674901977693
2216	22	-2.940217131222198
2216	23	-1.9035461642064817
2216	24	-2.137514449944625
2216	25	-1.9801745383911253
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43365.0
3	0.0
4	676.0
5	5697.0
6	6777.0
7	5352.0
8	4442.0
9	3985.0
10	3476.0
11	3207.0
12	2887.0
13	2894.0
14	2871.0
15	2923.0
16	3010.0
17	3230.0
18	3507.0
19	4179.0
20	5260.0
21	7070.0
22	9088.0
23	11320.0
24	13531.0
25	15957.0
26	18372.0
27	20330.0
28	23340.0
29	28465.0
30	36065.0
31	45666.0
32	58901.0
33	75356.0
34	98209.0
35	126983.0
36	173685.0
37	268262.0
38	870617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.80883406414177	19.23580453190898	13.23859226468915	27.716769139260105
2	13.883308311995506	20.623787925246596	39.69388019225944	25.79902357049845
3	17.10725227792559	24.769245702367648	30.0301898250583	28.093312194648462
4	11.625977843757111	17.32103548494229	37.531922606009935	33.52106406529066
5	12.865719923108843	37.979778960335494	36.03443663876648	13.120064477789183
6	31.97952019511408	37.42406377074096	17.820100316771452	12.776315717373512
7	27.76481168233304	31.457816812724047	22.481948024851746	18.295423480091163
8	25.192366414495265	36.98570906955278	20.43430201888222	17.387622497069735
9	26.338567795086536	14.772168686759525	19.79614634313971	39.09311717501423
10	14.607878802980089	28.884608676151796	34.88879475743154	21.618717763436575
11	34.36229376321648	22.044735909393207	24.714763029306233	18.878207298084085
12	23.18593172967876	24.541012873245972	31.793850832046406	20.479204565028862
13	28.995145904135715	21.599656315476903	25.585082578370677	23.820115202016705
14	20.903396798246092	21.831574880997064	27.47680923787345	29.788219082883387
15	23.211845544753583	30.598833426507216	23.04632293386712	23.142998094872087
16	22.390178279814872	27.693564640373445	27.529016274605084	22.3872408052066
17	22.045744815927783	28.43174502007902	27.454009953617835	22.068500210375362
18	21.9896562366223	27.25541828028712	29.239399882525113	21.51552560056547
19	23.65496014945634	26.791333085434132	27.27782282773345	22.275883937376083
20	23.74150499694349	27.178882413028894	27.516342862604464	21.563269727423155
21	23.054795209245814	26.569412027060864	26.92377571799838	23.452017045694948
22	23.358447588593457	27.74376889044636	26.682469267558407	22.215314253401775
23	22.377405168358674	27.470650163891175	27.335256389515944	22.816688278234206
24	23.242543134387347	26.90051638813034	27.69859339736002	22.158347080122294
25	22.942776714446072	27.0352459200004	27.38195566197169	22.640021703581837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	206.0
3	333.0
4	333.0
5	333.0
6	754.5
7	1176.0
8	1176.0
9	1176.0
10	1784.5
11	2393.0
12	2393.0
13	2393.0
14	4724.5
15	7056.0
16	7056.0
17	7056.0
18	13658.5
19	20261.0
20	20261.0
21	20261.0
22	33678.0
23	47095.0
24	47095.0
25	47095.0
26	68641.0
27	90187.0
28	90187.0
29	90187.0
30	117046.0
31	143905.0
32	143905.0
33	143905.0
34	165279.5
35	186654.0
36	186654.0
37	186654.0
38	203783.5
39	220913.0
40	220913.0
41	220913.0
42	234514.5
43	248116.0
44	248116.0
45	248116.0
46	259385.0
47	270654.0
48	270654.0
49	270654.0
50	261138.5
51	251623.0
52	251623.0
53	251623.0
54	224989.5
55	198356.0
56	198356.0
57	198356.0
58	170674.5
59	142993.0
60	142993.0
61	142993.0
62	116784.5
63	90576.0
64	90576.0
65	90576.0
66	70161.5
67	49747.0
68	49747.0
69	49747.0
70	36215.5
71	22684.0
72	22684.0
73	22684.0
74	15913.5
75	9143.0
76	9143.0
77	9143.0
78	6306.5
79	3470.0
80	3470.0
81	3470.0
82	2310.0
83	1150.0
84	1150.0
85	1150.0
86	730.5
87	311.0
88	311.0
89	311.0
90	190.0
91	69.0
92	69.0
93	69.0
94	39.0
95	9.0
96	9.0
97	9.0
98	5.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07909584833906184
2	1.4933136879621495E-4
3	0.0
4	0.028970285546465697
5	0.07177861126804733
6	0.07536256411915647
7	0.03613819124868402
8	0.07257504523496047
9	0.05196731634108281
10	0.11473626835842515
11	0.004927935170275093
12	0.014584697019096992
13	0.006769688718761745
14	0.009009659250704969
15	0.008263002406723894
16	0.021254831491994596
17	0.03185735867652586
18	7.466568439810748E-4
19	0.003683507096973302
20	0.006321694612373099
21	9.95542458641433E-5
22	1.4933136879621495E-4
23	0.0
24	4.977712293207165E-5
25	0.0034843986052450152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2008955.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.47754558977431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.13654964197005	56.37168326746961
2	12.535349236698998	17.41850596152995
3	3.432118620718555	7.153655338214601
4	1.2366183936693387	3.436688432932599
5	0.5594908117479157	1.9436024190137828
6	0.3026327681082393	1.2615709165919877
7	0.18175054058338194	0.8839307028543634
8	0.12683904919515135	0.7049972658415804
9	0.07906206747937	0.4943734557947769
>10	0.3699194180011123	4.428410726347824
>50	0.021079143880823204	1.0013577011812147
>100	0.014716202509779588	1.9863842512204763
>500	0.002726222344799526	1.2773794149925308
>1k	0.0011478830925471075	1.6374601460146687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3122	0.1554041777939277	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3074	0.15301487589318824	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2991	0.1488833746898263	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2959	0.14729050675600003	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2864	0.1425616800774532	No Hit
CCCATGTACTCTGCGTTGATACCAC	2753	0.13703641943199327	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2735	0.13614043121921596	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.977712293207165E-5	0.0
10	0.0	0.0	0.0	4.977712293207165E-5	0.0
11	0.0	0.0	0.0	4.977712293207165E-5	0.0
12	0.0	0.0	0.0	4.977712293207165E-5	0.0
13	0.0	0.0	0.0	4.977712293207165E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	750	0.0	12.17782	4
TAACCTC	155	2.5465852E-11	11.662227	5
CTAGGAC	345	0.0	11.573215	3
AGGACCT	1445	0.0	10.6002865	5
TGGCGAG	1810	0.0	10.539676	18
TAGAAAT	600	0.0	10.465315	4
GGCGAGG	745	0.0	10.446677	19
GTATTAA	220	0.0	10.371061	1
GGACCTG	1370	0.0	10.274481	6
GTCCTAC	1790	0.0	10.197245	1
TCCAACG	160	6.2700565E-9	10.084137	18
GTACCGT	85	6.52596E-4	10.070298	6
GAAATGT	605	0.0	10.06105	6
ATTTAGA	550	0.0	10.025359	1
ACGGAGG	95	1.639931E-4	10.002676	8
CTACACT	400	0.0	9.989618	4
TATGGCG	1995	0.0	9.94513	16
GCCCTAA	115	1.017915E-5	9.920145	1
TCTAGGA	405	0.0	9.857927	2
AGGACGT	1575	0.0	9.846154	5
>>END_MODULE