FastQCFastQC Report
Thu 26 May 2016
SRR1512933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049819
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34750.16952716313001295No Hit
GTCCTACAGTGGACATTTCTAAATT29790.14532990473793053No Hit
GTATCAACGCAGAGTACTTTTTTTT28120.13718284394866084No Hit
CCCATGTACTCTGCGTTGATACCAC27850.135865654479737No Hit
GTCCTAAAGTGTGTATTTCTCATTT27110.13225557963898277No Hit
CTGTAGGACGTGGAATATGGCAAGA24630.12015695044294156No Hit
CTTTAGGACGTGAAATATGGCGAGG24050.11732743232451255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6150.012.3532314
CCAACGA1201.0095391E-811.86894619
AATCCCG2050.011.57945919
GGCGAGG6900.011.28409919
GGACCGT855.3602635E-511.1691376
TCCAACG1451.2114469E-911.141767518
AGGACCG600.005893733411.0790355
ACTAGTT700.001487169910.86114912
CGTGCGC700.001497808410.851871510
AACCCGT700.00149994410.85001856
GTAGGAC15900.010.6313533
GTCCTAA9250.010.608191
TACTAGC1002.4083933E-510.4464562
GTCCTAC17100.010.4181651
GTATAAA3250.010.2596011
TGTAGGA17050.010.2487232
GAACAGT4450.010.2404676
TGGCGAG15700.010.04804618
GTCCTAG3250.09.966471
AGGACCT12350.09.9192295
Produced by FastQC (version 0.11.5)