##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512933_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2049819
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.748152397845857	31.0	30.0	34.0	22.0	34.0
2	28.85345876879861	31.0	30.0	34.0	16.0	34.0
3	29.082704863209873	31.0	30.0	34.0	22.0	34.0
4	32.4670100140549	35.0	33.0	37.0	23.0	37.0
5	32.590565313327666	35.0	33.0	37.0	25.0	37.0
6	32.61659151368975	37.0	33.0	37.0	25.0	37.0
7	32.43078388872383	36.0	33.0	37.0	23.0	37.0
8	32.42281001395733	36.0	33.0	37.0	23.0	37.0
9	33.70869720692412	38.0	34.0	39.0	22.0	39.0
10	33.58101520183001	38.0	34.0	39.0	19.0	39.0
11	33.63761093052606	38.0	34.0	39.0	21.0	39.0
12	33.52991508030709	38.0	34.0	39.0	19.0	39.0
13	33.522924707010716	38.0	34.0	39.0	18.0	39.0
14	34.49553741086408	39.0	34.0	41.0	17.0	41.0
15	34.427944613646375	39.0	34.0	41.0	17.0	41.0
16	34.31034008368544	39.0	34.0	41.0	17.0	41.0
17	34.24745892198287	39.0	34.0	41.0	17.0	41.0
18	33.94970043696541	39.0	33.0	41.0	11.0	41.0
19	34.18182776137795	39.0	34.0	41.0	18.0	41.0
20	34.25998734522414	39.0	34.0	41.0	17.0	41.0
21	34.183818668867836	39.0	34.0	41.0	15.0	41.0
22	34.100999161389375	39.0	34.0	41.0	10.0	41.0
23	33.827796503008315	39.0	34.0	41.0	2.0	41.0
24	33.82427228940701	39.0	34.0	41.0	2.0	41.0
25	33.74240652467364	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6766457686105305
1101	2	0.8836408723150697
1101	3	1.0331575668866044
1101	4	0.9621439215181837
1101	5	0.8577185937473928
1101	6	1.0535210948465732
1101	7	1.1135086488782662
1101	8	0.9224800587549993
1101	9	0.8159620993981136
1101	10	0.9637902609055828
1101	11	1.0454956436389438
1101	12	1.263301102687663
1101	13	1.0594873929943276
1101	14	0.9219199940892153
1101	15	0.8263901926366444
1101	16	1.2641765968140817
1101	17	1.3810974365421487
1101	18	1.5352871733026916
1101	19	0.8368831329848874
1101	20	1.1156181490560968
1101	21	1.1206185979560246
1101	22	1.097967301398846
1101	23	1.3201860908209824
1101	24	1.0955329185463043
1101	25	1.2827499191123053
1102	1	0.6221877952599755
1102	2	0.6867121924769997
1102	3	0.6665456988813112
1102	4	0.7690379647407184
1102	5	0.5676199405861695
1102	6	0.530514947618073
1102	7	0.8635639509939068
1102	8	0.8238249252394425
1102	9	0.8097248719550194
1102	10	0.8583206403222121
1102	11	0.8854696425616524
1102	12	0.7897695620044587
1102	13	0.764317969214865
1102	14	0.7465667690182727
1102	15	1.0383829507843814
1102	16	0.8843711128580267
1102	17	0.6880407352388644
1102	18	1.0795661837129842
1102	19	1.1075283132614828
1102	20	1.060809314793552
1102	21	0.8996069496851575
1102	22	1.2023211219990912
1102	23	1.3508641025440937
1102	24	1.12611428781112
1102	25	1.0825447381422961
1103	1	0.26179910584705723
1103	2	0.5050612824332035
1103	3	0.5138330641411528
1103	4	0.6054672605808875
1103	5	0.035466352104236876
1103	6	0.46916454145535624
1103	7	0.38290297154911457
1103	8	0.4788934671324938
1103	9	0.7536225462103019
1103	10	0.6513398845016098
1103	11	0.598249105558736
1103	12	0.7936434858820718
1103	13	0.8121651053620127
1103	14	1.0149296969541126
1103	15	0.9933634918549643
1103	16	1.0876530127502164
1103	17	0.982177927820814
1103	18	1.2561972613490724
1103	19	1.2733164432891684
1103	20	1.1013558637374246
1103	21	0.8212887549087213
1103	22	0.9907305479822455
1103	23	1.3319600116678956
1103	24	1.1126603412873237
1103	25	1.009623101702509
1104	1	0.6867953841359267
1104	2	0.5774674840952088
1104	3	0.6449108826530683
1104	4	0.918771522854982
1104	5	0.7888027053498163
1104	6	0.740833101466734
1104	7	1.0448804649933692
1104	8	0.9616728380142447
1104	9	0.877257540724699
1104	10	1.0835883394555452
1104	11	1.0963396907612903
1104	12	0.9670811075605883
1104	13	1.0454094533217173
1104	14	1.1460152929199694
1104	15	1.4081560497089995
1104	16	1.2451047758342142
1104	17	1.3227241184687486
1104	18	1.511757717143766
1104	19	1.3849499289775054
1104	20	1.3280212833525056
1104	21	1.3583427889693809
1104	22	1.2622632019715923
1104	23	1.4672950888127616
1104	24	1.413843541308779
1104	25	1.208153880826906
1105	1	0.4626336919879144
1105	2	0.5461263818430453
1105	3	0.530805468890069
1105	4	0.6941364930371492
1105	5	0.391911021111909
1105	6	0.23950077792043345
1105	7	0.5884737420369319
1105	8	0.6977094011533396
1105	9	0.5656961461450507
1105	10	0.6616835408363215
1105	11	0.580999500050126
1105	12	0.7365901588015902
1105	13	0.7635243787319936
1105	14	0.7847346070976045
1105	15	0.9156424461307608
1105	16	0.8562509508022629
1105	17	0.8245979988665653
1105	18	0.8738042340372871
1105	19	0.9414634700946891
1105	20	1.0211486643751755
1105	21	0.8805575993634847
1105	22	0.5355985295041776
1105	23	0.799174283279882
1105	24	0.9402423639179247
1105	25	0.970558084846175
1106	1	0.5761730090961485
1106	2	0.5879161962484147
1106	3	0.6175518161112343
1106	4	0.6803779147523841
1106	5	0.5702017913642692
1106	6	0.6727454607037089
1106	7	0.3887641529082515
1106	8	0.6024650002565366
1106	9	0.6507245357935219
1106	10	0.8977408887496168
1106	11	0.8004701056362222
1106	12	0.8459477724727904
1106	13	0.7277053265970395
1106	14	0.9756601876166329
1106	15	0.9907334174815077
1106	16	0.9846795887239352
1106	17	0.9164892100629771
1106	18	1.2106329918817949
1106	19	0.8279420736039356
1106	20	0.8323428507331272
1106	21	0.6877732149052136
1106	22	0.7196115950873434
1106	23	1.0481763228398862
1106	24	0.9215506755487439
1106	25	1.0012356633929684
1107	1	0.37464645996792356
1107	2	0.5195956424807946
1107	3	0.6884933759149483
1107	4	0.6528639305867401
1107	5	0.6996122480799087
1107	6	0.5198781060356694
1107	7	0.6106348692682602
1107	8	0.6330039922981712
1107	9	0.7904676947465532
1107	10	0.6501159847679858
1107	11	0.5881143620401872
1107	12	0.7731435324844469
1107	13	0.7064176620308942
1107	14	0.9881002404944397
1107	15	0.9714292427131923
1107	16	0.7596811715491754
1107	17	1.0530236199208929
1107	18	1.2790022699685508
1107	19	1.0633101891722987
1107	20	0.9866869050694262
1107	21	0.9269165593729909
1107	22	1.0184572959432927
1107	23	1.128792949599017
1107	24	1.0542146009400852
1107	25	1.0572717769810822
1108	1	0.3841673444888052
1108	2	0.5057750036601547
1108	3	0.39856708598865964
1108	4	0.6086378863606967
1108	5	0.47971052817818816
1108	6	0.6629984991560605
1108	7	0.6502540338874212
1108	8	0.6142693485635249
1108	9	0.6881949674738266
1108	10	0.7182978029498059
1108	11	0.6252765242023486
1108	12	0.7946423039832169
1108	13	0.7601645907778192
1108	14	0.9223754247696192
1108	15	0.8835914048753537
1108	16	0.7974575843255849
1108	17	0.7720039639012342
1108	18	0.9420858080520915
1108	19	1.0018851277472365
1108	20	0.8659866593691774
1108	21	0.8654914979479287
1108	22	0.7816636841496774
1108	23	1.0547757505818183
1108	24	1.003846050571532
1108	25	1.1749007696100762
1109	1	0.210721171042632
1109	2	0.23563592518774357
1109	3	0.48142949385106704
1109	4	0.5206589983818048
1109	5	0.5238865223541822
1109	6	0.4251265362841039
1109	7	0.5290358460025715
1109	8	0.5918765011706739
1109	9	0.7548874174996101
1109	10	0.9931635778155794
1109	11	0.4110152932744455
1109	12	0.6392990819733342
1109	13	0.6861891001356639
1109	14	0.5184343624952277
1109	15	0.8399553445726298
1109	16	0.7185658137671496
1109	17	0.5702067121039818
1109	18	-0.8294675635012823
1109	19	0.3878786970741359
1109	20	0.6044622561781097
1109	21	0.672194412984183
1109	22	0.7774139798999755
1109	23	0.355409717882452
1109	24	0.6641002441590587
1109	25	0.8691776222202634
1110	1	0.4095189742708172
1110	2	0.3941779964935286
1110	3	0.5143107476183957
1110	4	0.35406432957194767
1110	5	0.5085292120601608
1110	6	0.5100019632481363
1110	7	0.6056923548320725
1110	8	0.43442221143790505
1110	9	0.7018858324305128
1110	10	0.8600428893151459
1110	11	0.7634018268846106
1110	12	0.2939895470013525
1110	13	0.46699469475982625
1110	14	0.6803184738519121
1110	15	0.7709032605416368
1110	16	0.9163880181294388
1110	17	0.491809417883907
1110	18	1.0274090690082645
1110	19	0.8315568387480639
1110	20	0.9216116991714287
1110	21	0.9216156395151103
1110	22	0.900968999657529
1110	23	0.8129254011365319
1110	24	1.1189028918942014
1110	25	0.9738697616170455
1111	1	0.27905776960593087
1111	2	0.5346625756837469
1111	3	0.5410012822641654
1111	4	0.34312844515855545
1111	5	0.4588198183705998
1111	6	0.6924299227940338
1111	7	0.43213859190548476
1111	8	0.330338577856935
1111	9	0.7267395803257415
1111	10	0.6178020702122566
1111	11	0.5991329659429212
1111	12	0.6530931237060784
1111	13	0.6432241158398639
1111	14	0.48885993080072865
1111	15	0.6355107446410386
1111	16	0.5899491828625258
1111	17	0.5824210044820077
1111	18	0.8960642025216714
1111	19	0.5220664322484367
1111	20	0.368916340825308
1111	21	0.6846310519741365
1111	22	0.3305159483671858
1111	23	0.691510722329383
1111	24	0.7006715972081636
1111	25	0.2770056223446531
1112	1	-0.017784723774887823
1112	2	0.36646274003261325
1112	3	0.41560478295636116
1112	4	0.4535014726272024
1112	5	0.2946468749161184
1112	6	0.4633476088478403
1112	7	0.18118845001660588
1112	8	0.3638620289991721
1112	9	0.6043828451650484
1112	10	0.6219619592767742
1112	11	0.5899408510090964
1112	12	0.5877677068164715
1112	13	0.2257461810550936
1112	14	0.4465132681405777
1112	15	0.37699952001201353
1112	16	0.5839434352514843
1112	17	0.6705640079268917
1112	18	0.5679034380541381
1112	19	0.41122446625577425
1112	20	0.2913931564433554
1112	21	0.3577784094375218
1112	22	0.36423122928569285
1112	23	0.5851009631955293
1112	24	0.2774935776426801
1112	25	0.5059698871977574
1113	1	-0.09812846200054892
1113	2	0.08069338664305548
1113	3	-0.1969883248893325
1113	4	0.16257314755402064
1113	5	-0.08032921411961524
1113	6	-0.058270801145496876
1113	7	-0.09288626812384848
1113	8	0.2062902236489137
1113	9	0.033319459372513904
1113	10	-0.12186031704702316
1113	11	-0.07695478125476285
1113	12	0.05832678863544771
1113	13	0.0757621216979345
1113	14	0.13816326958972525
1113	15	-0.3052599936534648
1113	16	-0.2040518115386476
1113	17	-0.2989603812566557
1113	18	-0.1753072972442382
1113	19	-0.26995398199510134
1113	20	-0.3226143211672863
1113	21	-0.15968673852062665
1113	22	6.289609727616607E-5
1113	23	-0.4174582345553901
1113	24	0.08336263250747322
1113	25	0.0734886536818351
1114	1	-1.2345753177420846
1114	2	-1.0987465644046708
1114	3	-0.8958404541770584
1114	4	-1.0174364419071544
1114	5	-1.0693752505774157
1114	6	-1.5555971635698356
1114	7	-1.5610497736025657
1114	8	-1.0061584184937153
1114	9	-1.1930961719188105
1114	10	-1.375337107216744
1114	11	-2.1437716295905354
1114	12	-1.617584832410838
1114	13	-1.369780211170955
1114	14	-1.6865642032773138
1114	15	-1.6594628497350001
1114	16	-2.2176154046468213
1114	17	-4.397108187632192
1114	18	-3.1888471280245305
1114	19	-2.1969701051572166
1114	20	-1.3154737885999808
1114	21	-2.2325435385615755
1114	22	-2.2242493465264204
1114	23	-2.2402947708720724
1114	24	-1.8428054667082314
1114	25	-1.6898358368721702
1115	1	-1.1375125546667277
1115	2	-0.7517068430232001
1115	3	-0.9456531845519507
1115	4	-0.8508259178453308
1115	5	-0.7286208419733633
1115	6	-0.8399464666150571
1115	7	-1.8178774431642033
1115	8	-1.2338613869846888
1115	9	-0.979671557277328
1115	10	-0.853409535694027
1115	11	-1.735684634149571
1115	12	-1.2745262544182836
1115	13	-1.152363785244738
1115	14	-1.1240855570890318
1115	15	-2.4701802599620137
1115	16	-1.1379341998165913
1115	17	-1.1230512896890659
1115	18	-1.1687572697432032
1115	19	-1.4230512651630676
1115	20	-1.1595444231408436
1115	21	-1.9699806736606718
1115	22	-2.0002578447067734
1115	23	-1.233850977372164
1115	24	-1.126306239554033
1115	25	-1.4576779166466949
1116	1	-1.1218292610745735
1116	2	-1.9626144888554187
1116	3	-1.60188392114744
1116	4	-1.2199829806154163
1116	5	-1.724779502499473
1116	6	-1.468291283986126
1116	7	-1.1549825489484995
1116	8	-1.949083894540241
1116	9	-1.8285663479878167
1116	10	-2.823669351618186
1116	11	-1.4274800240725796
1116	12	-1.2745727472306356
1116	13	-1.9179252347948257
1116	14	-1.7302802594068254
1116	15	-1.2736453739628573
1116	16	-1.2219357225266378
1116	17	-1.6516608531008998
1116	18	-1.2427290248431149
1116	19	-1.767130856349418
1116	20	-1.9536866481290502
1116	21	-2.529811898177094
1116	22	-1.653633171224861
1116	23	-1.7253056454242852
1116	24	-2.1590488261342173
1116	25	-1.8520559682071323
1201	1	0.14380992016911875
1201	2	0.10710356861200054
1201	3	0.3701886879668308
1201	4	0.35524693617568914
1201	5	0.4771881130398512
1201	6	0.46492595354594357
1201	7	0.38101926797053665
1201	8	-0.09196495141078032
1201	9	0.24849190568889412
1201	10	0.41890739806691357
1201	11	-0.03171548248585765
1201	12	-0.6109231955221546
1201	13	-0.22070357497202053
1201	14	0.35737289862223065
1201	15	0.020860997053802066
1201	16	0.5595977261827088
1201	17	0.7152167205134177
1201	18	0.9725373755599449
1201	19	0.4940875629061807
1201	20	0.3608351651706698
1201	21	0.3294455747098084
1201	22	0.5794689610083594
1201	23	0.9157641774480823
1201	24	0.6698566588191142
1201	25	0.6247119567893478
1202	1	0.6883273714922851
1202	2	0.7307834422468673
1202	3	0.5173719858638464
1202	4	0.779075162149411
1202	5	0.7640237842166826
1202	6	0.8265198625176851
1202	7	0.6968572083659978
1202	8	0.852455130918635
1202	9	1.0427981184028567
1202	10	1.2131700859211278
1202	11	0.9166102918939387
1202	12	1.001325272167783
1202	13	0.9381247684983833
1202	14	1.1259117813411237
1202	15	0.777960708829319
1202	16	0.9153334546774943
1202	17	1.102800487988496
1202	18	1.392745784031554
1202	19	1.147133025950403
1202	20	1.1078652065192927
1202	21	1.1679355481555618
1202	22	1.0963821493473773
1202	23	1.2955667012769183
1202	24	1.4137935743369496
1202	25	1.2438620777087692
1203	1	0.4092610570825208
1203	2	0.6060537789389322
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2211	18	0.28776835500582365
2211	19	0.3247821002204816
2211	20	0.288218367194105
2211	21	0.220343036697777
2211	22	0.08637363292742606
2211	23	0.7221600840678448
2211	24	0.4738279353839303
2211	25	0.589982183844036
2212	1	-0.1862656559318978
2212	2	-0.22398890355533396
2212	3	-0.30189359253073533
2212	4	-0.40145193056127937
2212	5	-0.35760520607882995
2212	6	-0.4329728249892568
2212	7	-0.5178262148491406
2212	8	-0.47608298921426595
2212	9	-0.27979130261819307
2212	10	-0.36751346104313853
2212	11	-0.5820055839405072
2212	12	-0.24259132877762113
2212	13	-0.478442882497653
2212	14	-0.4925743739797497
2212	15	-0.5637291935365454
2212	16	-0.4164186070713569
2212	17	-0.4397903276655697
2212	18	-3.2028092651783666
2212	19	-1.4799805060166449
2212	20	-0.7481198807287583
2212	21	-0.8574920492747822
2212	22	-0.4511465511198267
2212	23	-0.46916057430747315
2212	24	-1.0581030971061693
2212	25	-0.6567282841985005
2213	1	-0.04540941505061724
2213	2	0.07487647770873451
2213	3	-0.3044136922099163
2213	4	-0.3053354933096344
2213	5	-0.20293824890120504
2213	6	-0.18476940508352868
2213	7	-0.022337966100344886
2213	8	-0.3990555322940281
2213	9	-0.13228751931233518
2213	10	-0.19023255945423045
2213	11	-0.2611708214858197
2213	12	-0.2578449714349844
2213	13	-0.11340776389285168
2213	14	-0.19263507138586533
2213	15	-0.24518105712672877
2213	16	-0.8451527170432698
2213	17	-0.29070158262509693
2213	18	-0.13719590478594057
2213	19	-0.0955401033898795
2213	20	-0.8688111157210656
2213	21	-0.47213037747730624
2213	22	-0.3159230410039413
2213	23	-0.926278421200422
2213	24	-0.5454035087122477
2213	25	-0.796178996584807
2214	1	-1.6456259884960147
2214	2	-3.670453933207078
2214	3	-1.8938445229123104
2214	4	-1.5705599284754186
2214	5	-1.6902190726024742
2214	6	-1.5693232810380557
2214	7	-1.6621054726210183
2214	8	-1.4210936256292364
2214	9	-1.744895279446208
2214	10	-1.7353001373502615
2214	11	-1.5147900413961324
2214	12	-2.050257237724832
2214	13	-1.9962589023477975
2214	14	-2.886788671628519
2214	15	-3.2877627345638913
2214	16	-2.5257580394219517
2214	17	-3.0124245652081427
2214	18	-2.2402616748273054
2214	19	-4.545113757017607
2214	20	-2.7794077679401425
2214	21	-2.6451772946357544
2214	22	-2.7902341324289885
2214	23	-2.6998115252820547
2214	24	-3.01863072988397
2214	25	-2.8002286150937756
2215	1	-0.8291402773553997
2215	2	-2.471236613595366
2215	3	-1.2291660082078515
2215	4	-0.7940012510345404
2215	5	-0.7245994993025704
2215	6	-1.439448658169141
2215	7	-1.8629694762873044
2215	8	-1.7437392381036005
2215	9	-1.3559476071565513
2215	10	-2.96676820223815
2215	11	-1.8066294077035856
2215	12	-1.9858433606487864
2215	13	-1.4033683989746777
2215	14	-2.809789904956684
2215	15	-2.4690911795145496
2215	16	-1.9755532710023402
2215	17	-2.3169746250773535
2215	18	-1.351021957616652
2215	19	-1.9055567217677805
2215	20	-1.949573357410351
2215	21	-1.6798239315138375
2215	22	-1.3130293617628794
2215	23	-2.0427980665041936
2215	24	-1.7279179830217686
2215	25	-1.7850445257010712
2216	1	-2.303607532661072
2216	2	-2.262624105676455
2216	3	-2.762320506148935
2216	4	-3.5043872979144908
2216	5	-2.5871782924606315
2216	6	-2.6613665192714535
2216	7	-2.5836879194295292
2216	8	-3.2305540543848785
2216	9	-3.363901162747304
2216	10	-2.9676466073696055
2216	11	-3.56588671289839
2216	12	-3.950542739014324
2216	13	-4.559486054185324
2216	14	-3.7445594646652687
2216	15	-3.5591688838224336
2216	16	-2.748833877590876
2216	17	-2.9521775471552765
2216	18	-3.0574189523901687
2216	19	-3.327125160950551
2216	20	-3.129384194439176
2216	21	-3.5212237968923645
2216	22	-4.0521462052852115
2216	23	-3.908290127796562
2216	24	-3.9041906509026667
2216	25	-4.058974383952577
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109858.0
3	5.0
4	880.0
5	5326.0
6	6573.0
7	6012.0
8	5282.0
9	4965.0
10	4561.0
11	4349.0
12	4084.0
13	4017.0
14	4196.0
15	4205.0
16	4235.0
17	4542.0
18	5096.0
19	6235.0
20	7547.0
21	9943.0
22	12415.0
23	15158.0
24	17381.0
25	19906.0
26	22089.0
27	24320.0
28	27796.0
29	32280.0
30	39945.0
31	49699.0
32	62457.0
33	79378.0
34	102140.0
35	133778.0
36	181634.0
37	278270.0
38	749262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.577850056983515	18.527965003113533	12.434204600195036	25.459980339707915
2	14.660121774812252	20.322513894392856	41.16293188770481	23.85443244309009
3	18.704675400222925	24.788298127770716	29.73225422964529	26.774772242361067
4	12.237173377606062	16.984486488570443	38.49233596715073	32.28600416667277
5	13.105679788386643	37.93062049174728	35.98388497916077	12.979814740705311
6	31.590007767702748	37.07058070408956	18.432973312671503	12.906438215536188
7	27.5635674161278	31.54352952427328	22.670512125539823	18.2223909340591
8	24.85122329680196	36.77131620294919	21.02235798143829	17.35510251881057
9	26.44750164068809	15.008599889238305	20.245139537777302	38.29875893229631
10	14.805713917671651	28.990481357943487	34.83057910114509	21.373225623239772
11	34.048798672054694	22.39814743432731	24.89321692017788	18.659836973440118
12	23.11534593729766	24.711131365759094	32.01915040100787	20.15437229593537
13	28.925751697641477	22.02242443257032	25.745549683550944	23.30627418623726
14	20.95081711226815	22.200176646545877	27.69403061548146	29.15497562570451
15	23.163343631254083	30.68798276078293	23.3206671022666	22.82800650569639
16	22.222357761462106	27.975331641478324	27.77537331166224	22.026937285397334
17	21.89655651946488	28.37232587541091	27.879403281628996	21.851714323495212
18	21.725040216362565	27.55067457026851	29.5585625446238	21.165722668745126
19	23.499835802316724	27.001272105082613	27.42745414541815	22.07143794718251
20	23.490975912997143	27.521367187728725	27.790128251921047	21.197528647353085
21	22.979780728549596	26.85416516211136	27.120886220485506	23.045167888853538
22	23.069221277100286	28.035185664983793	27.005114978450536	21.890478079465385
23	22.37316568800911	27.78844211444682	27.46425061700805	22.37414158053602
24	23.01440806605783	27.119162596115988	27.943275284377428	21.923154053448755
25	22.752328853483387	27.243206422956195	27.59740782541613	22.407056898144283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	270.0
2	424.5
3	579.0
4	579.0
5	579.0
6	1160.5
7	1742.0
8	1742.0
9	1742.0
10	2646.5
11	3551.0
12	3551.0
13	3551.0
14	6112.0
15	8673.0
16	8673.0
17	8673.0
18	16072.0
19	23471.0
20	23471.0
21	23471.0
22	37420.5
23	51370.0
24	51370.0
25	51370.0
26	73866.0
27	96362.0
28	96362.0
29	96362.0
30	125486.5
31	154611.0
32	154611.0
33	154611.0
34	175171.0
35	195731.0
36	195731.0
37	195731.0
38	211977.0
39	228223.0
40	228223.0
41	228223.0
42	239886.0
43	251549.0
44	251549.0
45	251549.0
46	260799.5
47	270050.0
48	270050.0
49	270050.0
50	259180.0
51	248310.0
52	248310.0
53	248310.0
54	221841.5
55	195373.0
56	195373.0
57	195373.0
58	168445.5
59	141518.0
60	141518.0
61	141518.0
62	115742.5
63	89967.0
64	89967.0
65	89967.0
66	69899.5
67	49832.0
68	49832.0
69	49832.0
70	36872.0
71	23912.0
72	23912.0
73	23912.0
74	16645.0
75	9378.0
76	9378.0
77	9378.0
78	6523.5
79	3669.0
80	3669.0
81	3669.0
82	2430.5
83	1192.0
84	1192.0
85	1192.0
86	773.0
87	354.0
88	354.0
89	354.0
90	228.5
91	103.0
92	103.0
93	103.0
94	63.0
95	23.0
96	23.0
97	23.0
98	14.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34749409582016755
2	0.038588772959954026
3	0.034685989348327825
4	0.03424692619201988
5	0.03897905132111664
6	0.014976932109615532
7	0.014733008133888895
8	0.021416525068798757
9	0.01824551338435247
10	0.01790401981833518
11	0.016440475963975354
12	0.017464956662027232
13	0.017464956662027232
14	0.02580715663187823
15	0.026246219788186177
16	0.019123639696968366
17	0.02000176600958426
18	0.10464338558672742
19	0.022441005766850635
20	0.0202456899853109
21	0.023758195235774476
22	0.01697710871057396
23	0.02014812039502024
24	0.016684399939701992
25	0.020489613961037535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2049819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.57034760768114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07483711207439	57.92049784842899
2	12.000281332285793	16.93728050018751
3	3.2316041380677416	6.841662820615839
4	1.1397017884946892	3.2171660553266443
5	0.5339162269409549	1.8839326864302377
6	0.2760522626747701	1.168866248090726
7	0.16585768047651162	0.8193243915241795
8	0.11483463762083958	0.6483136235443798
9	0.07417791454043406	0.4711285092538166
>10	0.3495242757318161	4.207854361021961
>50	0.021517110761132278	1.036655471551683
>100	0.014158151194266458	2.0402147167428075
>500	0.0023582460911845465	1.1717594578099901
>1k	0.0011791230455922208	1.635343309471175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3475	0.16952716313001295	No Hit
GTCCTACAGTGGACATTTCTAAATT	2979	0.14532990473793053	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2812	0.13718284394866084	No Hit
CCCATGTACTCTGCGTTGATACCAC	2785	0.135865654479737	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2711	0.13225557963898277	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2463	0.12015695044294156	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2405	0.11732743232451255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.878479514532746E-5	0.0
9	0.0	0.0	0.0	4.878479514532746E-5	0.0
10	0.0	0.0	0.0	4.878479514532746E-5	0.0
11	0.0	0.0	0.0	4.878479514532746E-5	0.0
12	0.0	0.0	0.0	4.878479514532746E-5	0.0
13	0.0	0.0	0.0	4.878479514532746E-5	4.878479514532746E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	615	0.0	12.353231	4
CCAACGA	120	1.0095391E-8	11.868946	19
AATCCCG	205	0.0	11.579459	19
GGCGAGG	690	0.0	11.284099	19
GGACCGT	85	5.3602635E-5	11.169137	6
TCCAACG	145	1.2114469E-9	11.1417675	18
AGGACCG	60	0.0058937334	11.079035	5
ACTAGTT	70	0.0014871699	10.861149	12
CGTGCGC	70	0.0014978084	10.8518715	10
AACCCGT	70	0.001499944	10.8500185	6
GTAGGAC	1590	0.0	10.631353	3
GTCCTAA	925	0.0	10.60819	1
TACTAGC	100	2.4083933E-5	10.446456	2
GTCCTAC	1710	0.0	10.418165	1
GTATAAA	325	0.0	10.259601	1
TGTAGGA	1705	0.0	10.248723	2
GAACAGT	445	0.0	10.240467	6
TGGCGAG	1570	0.0	10.048046	18
GTCCTAG	325	0.0	9.96647	1
AGGACCT	1235	0.0	9.919229	5
>>END_MODULE