FastQCFastQC Report
Thu 26 May 2016
SRR1512933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049819
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36900.18001589408625834No Hit
CCCATGTACTCTGCGTTGATACCAC33250.16220944385821381No Hit
GTACATGGGAAGCAGTGGTATCAAC32890.16045319123298205No Hit
GTCCTACAGTGGACATTTCTAAATT28610.13957329891078188No Hit
TATCAACGCAGAGTACTTTTTTTTT27200.13269464279529072No Hit
GTCCTAAAGTGTGTATTTCTCATTT25500.12440122762058503No Hit
CTTTAGGACGTGAAATATGGCGAGG25300.12342553171767849No Hit
CTGTAGGACGTGGAATATGGCAAGA24890.12142535511672006No Hit
GGTATCAACGCAGAGTACTTTTTTT22980.11210745924396252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG400.00527833114.25062210
TCCAACG1850.013.84957418
TAGGACC7200.012.9529464
CCAACGA1251.862827E-811.38742819
GGCGAGG8200.010.99395919
GCCGGTT1658.185452E-1110.92946211
GGACGTG15000.010.9081946
TGGCGAG18300.010.83776218
AGGACGT15850.010.6872445
GACGTGA9500.010.6043477
TTAGGAC11900.010.5470323
GACGTGG6300.010.4090297
TGTAGGA18050.010.3774912
GGCGTGC1752.3464963E-1010.3180118
GCACCGT1201.4976868E-610.3057066
ATAGGAC3050.010.287683
CTATAGG1951.4551915E-1110.241221
GTAGGAC17550.010.2397183
CTAGGAC2700.010.2126283
ATGTCGA1409.820724E-810.16933117
Produced by FastQC (version 0.11.5)