##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2049819 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.33388606506233 31.0 31.0 34.0 26.0 34.0 2 30.492850344347477 31.0 31.0 34.0 27.0 34.0 3 30.60937282755209 31.0 31.0 34.0 27.0 34.0 4 34.08517142245242 37.0 35.0 37.0 30.0 37.0 5 33.72164566725159 37.0 35.0 37.0 28.0 37.0 6 33.578897453872756 37.0 35.0 37.0 28.0 37.0 7 33.45689497462947 37.0 35.0 37.0 28.0 37.0 8 33.34380840454694 37.0 35.0 37.0 27.0 37.0 9 34.78627381246832 39.0 35.0 39.0 27.0 39.0 10 34.670920700803336 39.0 35.0 39.0 27.0 39.0 11 34.70025304673242 39.0 35.0 39.0 27.0 39.0 12 34.54248887340785 39.0 35.0 39.0 27.0 39.0 13 34.52718801025846 39.0 35.0 39.0 27.0 39.0 14 35.57563619031729 40.0 36.0 41.0 27.0 41.0 15 35.5881782733012 40.0 36.0 41.0 27.0 41.0 16 35.562882381322446 40.0 36.0 41.0 27.0 41.0 17 35.536196610530006 40.0 36.0 41.0 27.0 41.0 18 35.46034991382166 40.0 36.0 41.0 26.0 41.0 19 35.44070915529615 40.0 36.0 41.0 26.0 41.0 20 35.412411534872106 40.0 36.0 41.0 25.0 41.0 21 35.357762807350305 40.0 36.0 41.0 25.0 41.0 22 35.3157488539232 40.0 36.0 41.0 25.0 41.0 23 35.21209238474226 40.0 36.0 41.0 25.0 41.0 24 35.19484744750634 40.0 36.0 41.0 25.0 41.0 25 35.100387400058246 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.632711083034966 1101 2 0.711835035894751 1101 3 0.6643736211360718 1101 4 0.507794845939749 1101 5 0.7966390501335923 1101 6 0.8662893698111276 1101 7 0.7789178525923077 1101 8 0.768401848603709 1101 9 0.6870385445687077 1101 10 1.0328440120532534 1101 11 0.9391015875720043 1101 12 1.0668483760487248 1101 13 1.1536997515793743 1101 14 1.2081486260661833 1101 15 1.1755785844563746 1101 16 1.2949217819047831 1101 17 1.2731742306682534 1101 18 0.8402650355641725 1101 19 1.0505856079668376 1101 20 1.0471021934278113 1101 21 1.0882344076329105 1101 22 1.0670499240444116 1101 23 1.062079769781974 1101 24 0.9884622114243697 1101 25 1.0426919730363409 1102 1 0.5987086122682719 1102 2 0.577120327857223 1102 3 0.5126530583925373 1102 4 0.5036650676786536 1102 5 0.6020036304338703 1102 6 0.5560574247970536 1102 7 0.7626052076999841 1102 8 0.6617949404014851 1102 9 0.81446458428222 1102 10 0.8566110395772526 1102 11 0.7236612576464836 1102 12 0.8020734725276881 1102 13 0.8635065320599224 1102 14 0.8841246292559077 1102 15 0.852993636410261 1102 16 0.9054877610397654 1102 17 0.8370259859122484 1102 18 0.7954894739194103 1102 19 0.9016665346590145 1102 20 0.8891663060969321 1102 21 0.9878125129569924 1102 22 0.8827182928017265 1102 23 0.9694420047120005 1102 24 0.9751667627651344 1102 25 1.038411573715102 1103 1 0.45896907075144 1103 2 0.45191325291992257 1103 3 0.5064037922824554 1103 4 0.5152386321950502 1103 5 0.5051723806121089 1103 6 0.4650336609213923 1103 7 0.5059308309393487 1103 8 0.473061745275416 1103 9 0.5525373571362735 1103 10 0.6908562993207497 1103 11 0.604633015684918 1103 12 0.6497772457849607 1103 13 0.6797196915472341 1103 14 0.7217777769218117 1103 15 0.5653434978833047 1103 16 0.6599509534855201 1103 17 0.8412763312528071 1103 18 0.6819678040086572 1103 19 0.7959059060301001 1103 20 0.9315788256576027 1103 21 0.49314975116824655 1103 22 0.7407569614043865 1103 23 0.7962195254189908 1103 24 0.7996897127524321 1103 25 0.8166836012972851 1104 1 0.6121841156577617 1104 2 0.6931179246969066 1104 3 0.6197707528006084 1104 4 0.5714511776470417 1104 5 0.6813388915136613 1104 6 0.7642468717445396 1104 7 0.6958630364687366 1104 8 0.6257562990335259 1104 9 0.8198821513124344 1104 10 0.7954964579648589 1104 11 0.8845878546023798 1104 12 0.8291443784042087 1104 13 0.8965220033256145 1104 14 1.0961825150265199 1104 15 1.0897839973194863 1104 16 1.0520268646267112 1104 17 1.0307492681560149 1104 18 1.1419532521929199 1104 19 1.1199321862463805 1104 20 1.1201099926575893 1104 21 0.9651660231972627 1104 22 1.027434511853393 1104 23 0.9029999795604766 1104 24 1.054178674700644 1104 25 1.1362686561816844 1105 1 0.3708793042535454 1105 2 0.4639825759861189 1105 3 0.44452924079417855 1105 4 0.29839194748927866 1105 5 0.3898832220263202 1105 6 0.5100869899911231 1105 7 0.5136193475970074 1105 8 0.6661308311246046 1105 9 0.618411974269506 1105 10 0.6758191278625816 1105 11 0.6841185183437872 1105 12 0.6027137884937162 1105 13 0.6175249716895834 1105 14 0.7036295317060635 1105 15 0.6142216404492373 1105 16 0.686761846769194 1105 17 0.45283461825754046 1105 18 0.7679948268555918 1105 19 0.7981836866535943 1105 20 0.9282398843218189 1105 21 0.902044444220877 1105 22 0.9224447032526015 1105 23 0.804506803674542 1105 24 0.9597359029328416 1105 25 0.8886363448836008 1106 1 0.48019226168336004 1106 2 0.47202622092347113 1106 3 0.4865987137003849 1106 4 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-2.5091021593599265 2216 21 -2.499166554219066 2216 22 -2.6666075588378817 2216 23 -1.841453609741997 2216 24 -2.070394264773867 2216 25 -1.809863557612502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56264.0 3 2.0 4 940.0 5 7789.0 6 9171.0 7 7277.0 8 5632.0 9 4966.0 10 4340.0 11 4023.0 12 3591.0 13 3470.0 14 3358.0 15 3427.0 16 3460.0 17 3594.0 18 4048.0 19 4646.0 20 5955.0 21 7760.0 22 9853.0 23 11976.0 24 14156.0 25 16421.0 26 18581.0 27 20689.0 28 23585.0 29 28029.0 30 35679.0 31 45420.0 32 57884.0 33 74540.0 34 97469.0 35 127450.0 36 173854.0 37 273106.0 38 877414.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.41504096675887 19.544185610517488 13.393459574679994 27.64731384804364 2 13.518517253726916 20.821019231951325 39.69571932727686 25.964744187044897 3 16.843584726261195 24.802970408606807 30.415319596510713 27.938125268621278 4 11.46073721272396 17.578670752804136 37.609999975600296 33.35059205887161 5 12.729111061530626 37.989852057587356 36.14802931410826 13.133007566773763 6 31.56127331315301 37.670735279757835 17.95898838004101 12.80900302704814 7 27.262137040532767 31.6281920765542 22.75025889945526 18.359411983457765 8 24.803978572261986 37.14484617791779 20.71238978447773 17.338785465342493 9 26.090103686450377 14.873438882687346 20.08909723039107 38.94736020047121 10 14.440358808782971 29.25145283174599 34.90014666287026 21.408041696600772 11 34.026321618248716 22.356885470322315 24.761211520391548 18.85558139103742 12 22.785262831253057 24.823352846407484 32.11426223893253 20.277122083406926 13 28.82106764788247 21.863568460195676 25.80862974227473 23.50673414964712 14 20.733029965501544 22.117749457592172 27.66633636749954 29.482884209406745 15 22.955353788658662 30.84018402962428 23.31487507745149 22.889587104265566 16 22.07208328148978 27.957071095204288 27.717639251793784 22.25320637151215 17 21.64101352871393 28.45841566233017 27.9506069938615 21.9499638150944 18 21.634854387167437 27.63967928343704 29.51192442134175 21.21354190805377 19 23.331347728238473 26.992910853161334 27.509363323288223 22.166378095311973 20 23.346260409434926 27.410995641807617 27.890235804894658 21.352508143862796 21 22.794848703345767 26.812920586941058 27.269840092808423 23.122390616904752 22 22.949969533776528 27.877106718619427 27.154942577632767 22.01798116997128 23 22.139027884901058 27.651319457961897 27.624243896656242 22.585408760480803 24 22.882361808530412 27.109271599102165 28.06257528103701 21.945791311330414 25 22.666866365427264 27.161552069175134 27.693079267073024 22.478502298324578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 206.0 1 206.0 2 335.0 3 464.0 4 464.0 5 464.0 6 1126.0 7 1788.0 8 1788.0 9 1788.0 10 2592.5 11 3397.0 12 3397.0 13 3397.0 14 6086.0 15 8775.0 16 8775.0 17 8775.0 18 16177.5 19 23580.0 20 23580.0 21 23580.0 22 37980.5 23 52381.0 24 52381.0 25 52381.0 26 75423.5 27 98466.0 28 98466.0 29 98466.0 30 127204.5 31 155943.0 32 155943.0 33 155943.0 34 175835.5 35 195728.0 36 195728.0 37 195728.0 38 211795.5 39 227863.0 40 227863.0 41 227863.0 42 239543.0 43 251223.0 44 251223.0 45 251223.0 46 260376.5 47 269530.0 48 269530.0 49 269530.0 50 260028.5 51 250527.0 52 250527.0 53 250527.0 54 222942.0 55 195357.0 56 195357.0 57 195357.0 58 167913.0 59 140469.0 60 140469.0 61 140469.0 62 114813.0 63 89157.0 64 89157.0 65 89157.0 66 68892.0 67 48627.0 68 48627.0 69 48627.0 70 35575.5 71 22524.0 72 22524.0 73 22524.0 74 15744.0 75 8964.0 76 8964.0 77 8964.0 78 6187.0 79 3410.0 80 3410.0 81 3410.0 82 2237.5 83 1065.0 84 1065.0 85 1065.0 86 686.0 87 307.0 88 307.0 89 307.0 90 185.0 91 63.0 92 63.0 93 63.0 94 34.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07673848276360011 2 4.878479514532746E-5 3 0.0 4 0.029953864219231064 5 0.0742504582111884 6 0.07898258334028517 7 0.0371252291055942 8 0.07054281378014352 9 0.05102889572201253 10 0.11054634579931204 11 0.004732125129096764 12 0.012342553171767849 13 0.005707821032003313 14 0.007903136813543049 15 0.007268934476653791 16 0.018733361335805745 17 0.030344142580393682 18 0.0016586830349411337 19 0.003219796479591613 20 0.005659036236857986 21 2.4392397572663732E-4 22 3.902783611626197E-4 23 0.0 24 0.0 25 0.003171011684446285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2049819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.17669694718309 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85068451908285 55.92983300948903 2 12.770156280766912 17.66794462005559 3 3.478506497386094 7.218947693954555 4 1.2537591303010738 3.4692366160640495 5 0.5689670706114743 1.9679631308308239 6 0.28869204743803834 1.198245736600979 7 0.18017641645091495 0.8724806550498078 8 0.12577258133650696 0.6960425394704383 9 0.07952095497151537 0.4950897302713601 >10 0.36439607905531096 4.344381639922994 >50 0.02020526554014176 0.9707994617870631 >100 0.015346665499312288 2.093243212885997 >500 0.0024736519369565743 1.1893753215757332 >1k 0.00134283962291917 1.886416632041568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3690 0.18001589408625834 No Hit CCCATGTACTCTGCGTTGATACCAC 3325 0.16220944385821381 No Hit GTACATGGGAAGCAGTGGTATCAAC 3289 0.16045319123298205 No Hit GTCCTACAGTGGACATTTCTAAATT 2861 0.13957329891078188 No Hit TATCAACGCAGAGTACTTTTTTTTT 2720 0.13269464279529072 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2550 0.12440122762058503 No Hit CTTTAGGACGTGAAATATGGCGAGG 2530 0.12342553171767849 No Hit CTGTAGGACGTGGAATATGGCAAGA 2489 0.12142535511672006 No Hit GGTATCAACGCAGAGTACTTTTTTT 2298 0.11210745924396252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.878479514532746E-5 0.0 0.0 0.0 0.0 9 4.878479514532746E-5 0.0 0.0 0.0 0.0 10 4.878479514532746E-5 0.0 0.0 0.0 0.0 11 4.878479514532746E-5 0.0 0.0 4.878479514532746E-5 0.0 12 4.878479514532746E-5 0.0 0.0 4.878479514532746E-5 4.878479514532746E-5 13 4.878479514532746E-5 0.0 0.0 4.878479514532746E-5 4.878479514532746E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTCG 40 0.005278331 14.250622 10 TCCAACG 185 0.0 13.849574 18 TAGGACC 720 0.0 12.952946 4 CCAACGA 125 1.862827E-8 11.387428 19 GGCGAGG 820 0.0 10.993959 19 GCCGGTT 165 8.185452E-11 10.929462 11 GGACGTG 1500 0.0 10.908194 6 TGGCGAG 1830 0.0 10.837762 18 AGGACGT 1585 0.0 10.687244 5 GACGTGA 950 0.0 10.604347 7 TTAGGAC 1190 0.0 10.547032 3 GACGTGG 630 0.0 10.409029 7 TGTAGGA 1805 0.0 10.377491 2 GGCGTGC 175 2.3464963E-10 10.318011 8 GCACCGT 120 1.4976868E-6 10.305706 6 ATAGGAC 305 0.0 10.28768 3 CTATAGG 195 1.4551915E-11 10.24122 1 GTAGGAC 1755 0.0 10.239718 3 CTAGGAC 270 0.0 10.212628 3 ATGTCGA 140 9.820724E-8 10.169331 17 >>END_MODULE