FastQCFastQC Report
Thu 26 May 2016
SRR1512932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236862
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC45820.20484053106539427No Hit
GTCCTACAGTGGACATTTCTAAATT44640.19956528386641642No Hit
GTCCTAAAGTGTGTATTTCTCATTT41340.18481247390317326No Hit
CTGTAGGACGTGGAATATGGCAAGA40070.17913487734156153No Hit
CTTTAGGACGTGAAATATGGCGAGG38170.17064083524151244No Hit
CCCATGTACTCTGCGTTGATACCAC36210.16187856023304076No Hit
GTATCAACGCAGAGTACTTTTTTTT34210.15293746328562066No Hit
GTCCTACAGTGTGCATTTCTCATTT27440.12267185011860365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC250.005922296619.0609551
TAGGACC7500.012.6632534
GGCGAGG10750.011.4816819
GTCCTAA12800.011.1685291
CGACTGA600.005864364611.08654513
AGGACCT15650.010.9230825
TCCAACG2100.010.86004518
CCTATAC1605.475158E-1010.6846193
GGACCTG15600.010.58974556
GTCCTAC24300.010.510981
ATTTAGA7350.010.3733091
GACGTGA12750.010.2761347
CTTACAC2500.010.2572343
TGTAGGA23550.010.2439812
GTAGGAC22900.010.2439633
TGGCGAG24600.010.19784718
TTAGGAC15400.010.1758283
GTGTAGG3100.010.1453481
AGGACGT23150.010.1328835
GGACGTG22850.010.0967346
Produced by FastQC (version 0.11.5)