##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512932_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236862 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.991576145510987 31.0 30.0 34.0 23.0 34.0 2 29.100098262655454 31.0 30.0 34.0 22.0 34.0 3 29.323774108550282 31.0 30.0 34.0 23.0 34.0 4 32.721252808622076 35.0 33.0 37.0 25.0 37.0 5 32.85468482186206 35.0 35.0 37.0 26.0 37.0 6 32.881806298287515 37.0 35.0 37.0 26.0 37.0 7 32.707749516957236 36.0 33.0 37.0 25.0 37.0 8 32.70465723857797 37.0 33.0 37.0 25.0 37.0 9 34.01097698472235 39.0 34.0 39.0 25.0 39.0 10 33.88112230437103 38.0 34.0 39.0 23.0 39.0 11 33.93816784406012 38.0 34.0 39.0 25.0 39.0 12 33.83514226626408 38.0 34.0 39.0 23.0 39.0 13 33.828759664208164 38.0 34.0 39.0 23.0 39.0 14 34.81473778892037 39.0 34.0 41.0 22.0 41.0 15 34.75626793248757 39.0 34.0 41.0 22.0 41.0 16 34.64148615337021 39.0 34.0 41.0 19.0 41.0 17 34.590530841866865 39.0 34.0 41.0 19.0 41.0 18 34.276839161289345 39.0 33.0 41.0 17.0 41.0 19 34.51500181951323 39.0 34.0 41.0 18.0 41.0 20 34.60233577216654 39.0 34.0 41.0 18.0 41.0 21 34.52884308464268 39.0 34.0 41.0 18.0 41.0 22 34.44695962468852 39.0 34.0 41.0 18.0 41.0 23 34.177917994047014 39.0 34.0 41.0 11.0 41.0 24 34.17755364434641 39.0 34.0 41.0 10.0 41.0 25 34.09434064327616 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2650233731265992 1101 2 0.47298969591160755 1101 3 0.5246777902603235 1101 4 0.5275410310274111 1101 5 0.350923798159414 1101 6 0.5322896288264829 1101 7 0.463256138012369 1101 8 0.3053543757302606 1101 9 0.19129254200764478 1101 10 0.2659437670064122 1101 11 0.548014270204213 1101 12 0.685938739029595 1101 13 0.5094075957446549 1101 14 0.50730775100687 1101 15 0.2883463334279668 1101 16 0.6175714964222436 1101 17 0.5367922263877674 1101 18 0.7225336264739255 1101 19 0.12187964640109072 1101 20 0.34426935602729714 1101 21 0.30675160644369015 1101 22 0.3465833401267062 1101 23 0.6573181273748148 1101 24 0.2545694817223847 1101 25 0.46615880536525367 1102 1 0.5245076720258375 1102 2 0.6325670249078001 1102 3 0.6614088089146435 1102 4 0.782563570799546 1102 5 0.5278891557236562 1102 6 0.6000594796367054 1102 7 0.8229257566344117 1102 8 0.8234116050590359 1102 9 0.794236369028205 1102 10 0.891154822307314 1102 11 0.7790863562442638 1102 12 0.9369937841369094 1102 13 0.7396163597923646 1102 14 0.9167450215765243 1102 15 0.9424886886612427 1102 16 1.0676568441314842 1102 17 0.883374270670295 1102 18 1.1302749657808349 1102 19 1.1381020219905906 1102 20 1.0033042899394076 1102 21 0.8407037211870971 1102 22 1.1598419969491474 1102 23 1.3816044688972582 1102 24 1.2417613510803918 1102 25 1.1256486241399983 1103 1 0.4130753374905929 1103 2 0.5704252648480406 1103 3 0.6172359537473575 1103 4 0.7630368666686991 1103 5 0.41538061028661133 1103 6 0.7794805864343033 1103 7 0.6671709240920691 1103 8 0.6493616807853897 1103 9 0.9441573520146633 1103 10 0.8372365080474822 1103 11 0.8473137837448164 1103 12 1.0192308746654746 1103 13 1.014860136122799 1103 14 1.201239506016222 1103 15 1.154479942082503 1103 16 1.3241825964384404 1103 17 1.1235891179712496 1103 18 1.3230247331721898 1103 19 1.4160791864438735 1103 20 1.1776173848807545 1103 21 1.0511671203912485 1103 22 1.2797903171074907 1103 23 1.5613041302059045 1103 24 1.2499603637173564 1103 25 1.1683002064135337 1104 1 0.2678307869589709 1104 2 0.2297659982551039 1104 3 0.30407145848408845 1104 4 0.4310969960693001 1104 5 0.20180550668927566 1104 6 0.28308211182104515 1104 7 0.39446386402061506 1104 8 0.3837490088475164 1104 9 0.458614784740611 1104 10 0.4340459197773754 1104 11 0.5044418926408909 1104 12 0.407772226676947 1104 13 0.3941431353102942 1104 14 0.5941184042986976 1104 15 0.8166750835802432 1104 16 0.5502712469051687 1104 17 0.5290753483516681 1104 18 0.73150485764927 1104 19 0.7215988293531268 1104 20 0.5609816443247269 1104 21 0.658871400195082 1104 22 0.5715840274147013 1104 23 0.9031272049065322 1104 24 0.7243469273600027 1104 25 0.519926837637442 1105 1 0.25719381898615623 1105 2 0.47526943644942676 1105 3 0.49369841066290476 1105 4 0.46066518439806003 1105 5 0.24964414529442536 1105 6 -0.005253482126569509 1105 7 0.3357583576167542 1105 8 0.43983511178688417 1105 9 0.24515033173856438 1105 10 0.4769870860280818 1105 11 0.4203542333322332 1105 12 0.45907924153073765 1105 13 0.4299485988453142 1105 14 0.455153777760664 1105 15 0.6872893201572694 1105 16 0.6736124630954521 1105 17 0.544488172075063 1105 18 0.573674546347128 1105 19 0.6406555855191058 1105 20 0.5824102474994461 1105 21 0.6312531987551537 1105 22 0.3434424301321286 1105 23 0.5516363105962157 1105 24 0.5990787546676586 1105 25 0.6195210583841018 1106 1 0.7072969902977988 1106 2 0.8046024247624537 1106 3 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1107 16 0.7797850410544811 1107 17 0.9735038197867567 1107 18 1.3018504315697825 1107 19 1.130599032488135 1107 20 0.9800045368791288 1107 21 0.8791473192407722 1107 22 1.0210524908045215 1107 23 1.0325583427390512 1107 24 1.0878052227808155 1107 25 1.079216578277702 1108 1 0.3900254437943502 1108 2 0.4005149442536933 1108 3 0.23287514974481027 1108 4 0.40572778869302795 1108 5 0.34473664292559647 1108 6 0.4408682672658131 1108 7 0.42642586098463653 1108 8 0.37514554198555317 1108 9 0.5221645416100742 1108 10 0.4615666514741932 1108 11 0.37521014432970645 1108 12 0.4588444656683208 1108 13 0.5021367910555625 1108 14 0.7073508208960462 1108 15 0.728781504962086 1108 16 0.6675988292365247 1108 17 0.6238817510176702 1108 18 0.8000897967877236 1108 19 0.736437268943213 1108 20 0.7634442915605035 1108 21 0.5448521691131418 1108 22 0.45770779554109 1108 23 0.5851746825567332 1108 24 0.8277703475375944 1108 25 0.843535241718449 1109 1 0.27990751458433394 1109 2 0.1789928634261173 1109 3 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-2.8359261547890995 2216 15 -2.8153176331142618 2216 16 -1.92109522323247 2216 17 -2.0259223512338806 2216 18 -2.052348662498133 2216 19 -2.2931354578066987 2216 20 -2.1682248418687635 2216 21 -2.589215166565104 2216 22 -3.100717956006232 2216 23 -3.0010118985631813 2216 24 -2.951572669519095 2216 25 -2.9465069587615957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106797.0 3 2.0 4 827.0 5 5171.0 6 6518.0 7 5849.0 8 5269.0 9 5025.0 10 4744.0 11 4415.0 12 4147.0 13 4257.0 14 4217.0 15 4296.0 16 4402.0 17 4689.0 18 5239.0 19 6206.0 20 8061.0 21 10460.0 22 13204.0 23 15841.0 24 18295.0 25 21022.0 26 23499.0 27 26076.0 28 29952.0 29 35680.0 30 43284.0 31 54486.0 32 68629.0 33 87330.0 34 112216.0 35 146567.0 36 200432.0 37 306281.0 38 833477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21508573253888 18.72607355784092 12.551868197241141 25.50697251237905 2 14.462618828689905 20.184821770382005 41.69361965516871 23.658939745759376 3 18.458905426714356 25.05574472538176 29.77045605580465 26.714893792099232 4 12.253049980322707 17.01906908518479 38.52438195413402 32.20349898035849 5 12.899728754888784 38.139254064763406 36.14478630223369 12.816230878114121 6 31.461577465242165 37.19506687759392 18.469519431032957 12.873836226130955 7 27.600636893405312 31.65347704720049 22.80135623776817 17.94452982162603 8 24.68303318705357 37.20667658161454 20.925622687642477 17.184667543689418 9 26.533805219514267 15.176923782035088 20.382218863672442 37.907052134778205 10 14.994298949665316 28.840562861345987 34.92217507366651 21.24296311532219 11 33.70508237366725 22.382162842534278 25.279381878911046 18.63337290488742 12 23.417513513706872 24.78697103825601 31.990763863706178 19.80475158433094 13 28.93949522342478 22.1823571890084 25.74615403532855 23.13199355223827 14 20.658084843693338 22.492786001018658 27.77133601964336 29.077793135644647 15 22.86730276387921 31.361557184578942 23.189629382521833 22.581510669020023 16 21.839349776135652 28.033369716226847 28.36568966450083 21.761590843136666 17 21.654638299394556 28.796671406469383 28.05587601391534 21.492814280220713 18 21.580666541063508 27.390379543809917 30.095755254635048 20.933198660491527 19 23.462208517191605 27.14655836989631 27.610489356640628 21.780743756271452 20 23.635560388771832 27.49457157472625 27.99529238374942 20.87457565275249 21 22.603283371320202 27.023846095714394 27.319960345195344 23.052910187770063 22 23.170818954068924 28.38451144185531 26.945303200893562 21.4993664031822 23 21.862485400565554 28.206107348098808 27.86162329613645 22.06978395519919 24 23.043105776470945 27.229111109918748 28.246660335209516 21.48112277840079 25 22.581676492051457 27.406831742670313 27.848213902442513 22.16327786283572 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 285.0 1 285.0 2 446.5 3 608.0 4 608.0 5 608.0 6 1292.5 7 1977.0 8 1977.0 9 1977.0 10 2956.0 11 3935.0 12 3935.0 13 3935.0 14 6991.0 15 10047.0 16 10047.0 17 10047.0 18 18448.5 19 26850.0 20 26850.0 21 26850.0 22 42418.5 23 57987.0 24 57987.0 25 57987.0 26 83210.5 27 108434.0 28 108434.0 29 108434.0 30 140753.5 31 173073.0 32 173073.0 33 173073.0 34 195926.5 35 218780.0 36 218780.0 37 218780.0 38 235392.5 39 252005.0 40 252005.0 41 252005.0 42 264023.5 43 276042.0 44 276042.0 45 276042.0 46 288197.5 47 300353.0 48 300353.0 49 300353.0 50 286117.0 51 271881.0 52 271881.0 53 271881.0 54 238935.0 55 205989.0 56 205989.0 57 205989.0 58 176712.5 59 147436.0 60 147436.0 61 147436.0 62 120144.0 63 92852.0 64 92852.0 65 92852.0 66 71773.5 67 50695.0 68 50695.0 69 50695.0 70 37060.0 71 23425.0 72 23425.0 73 23425.0 74 16380.5 75 9336.0 76 9336.0 77 9336.0 78 6353.5 79 3371.0 80 3371.0 81 3371.0 82 2224.0 83 1077.0 84 1077.0 85 1077.0 86 704.5 87 332.0 88 332.0 89 332.0 90 203.5 91 75.0 92 75.0 93 75.0 94 42.5 95 10.0 96 10.0 97 10.0 98 8.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35294980199940806 2 0.0388937717212774 3 0.03469145615598995 4 0.034959689064412554 5 0.0392067101144371 6 0.017345728077994976 7 0.01703278968483527 8 0.022710386246447032 9 0.019983351677483906 10 0.019312769406427396 11 0.018195132287999884 12 0.019178652952216095 13 0.019089241982741895 14 0.02615270857120377 15 0.027762106021739383 16 0.02069863943327751 17 0.02154804364328242 18 0.10331437522743915 19 0.024051550788560045 20 0.021726865582230824 21 0.025169187906987557 22 0.018955125528530594 23 0.021190399765385618 24 0.018329248742211188 25 0.021369221704334015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2236862.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.03216625744379 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19888192413303 57.565657650868765 2 11.986557275688664 16.78889143970802 3 3.169655286674037 6.659334780454255 4 1.141732937797022 3.198321236856031 5 0.5111104088581966 1.789708456453365 6 0.2685415210075616 1.1283926667737032 7 0.16531146666268662 0.8103984082308203 8 0.1093665319490375 0.6127340118764026 9 0.07333814049285436 0.4622425963206613 >10 0.33630919211510985 4.058802910036079 >50 0.02043200603612683 1.0040168603312913 >100 0.014732042887435638 2.1901028939185143 >500 0.002495545432257037 1.2753615798505364 >1k 0.0015357202660033393 2.4560345083217348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4582 0.20484053106539427 No Hit GTCCTACAGTGGACATTTCTAAATT 4464 0.19956528386641642 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4134 0.18481247390317326 No Hit CTGTAGGACGTGGAATATGGCAAGA 4007 0.17913487734156153 No Hit CTTTAGGACGTGAAATATGGCGAGG 3817 0.17064083524151244 No Hit CCCATGTACTCTGCGTTGATACCAC 3621 0.16187856023304076 No Hit GTATCAACGCAGAGTACTTTTTTTT 3421 0.15293746328562066 No Hit GTCCTACAGTGTGCATTTCTCATTT 2744 0.12267185011860365 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.941096947420091E-5 2 0.0 4.4705484737100455E-5 0.0 0.0 8.941096947420091E-5 3 0.0 4.4705484737100455E-5 0.0 8.941096947420091E-5 8.941096947420091E-5 4 0.0 4.4705484737100455E-5 0.0 8.941096947420091E-5 8.941096947420091E-5 5 0.0 4.4705484737100455E-5 0.0 8.941096947420091E-5 1.3411645421130137E-4 6 0.0 4.4705484737100455E-5 0.0 8.941096947420091E-5 1.3411645421130137E-4 7 0.0 4.4705484737100455E-5 0.0 8.941096947420091E-5 1.3411645421130137E-4 8 0.0 4.4705484737100455E-5 0.0 1.3411645421130137E-4 1.3411645421130137E-4 9 0.0 4.4705484737100455E-5 0.0 1.3411645421130137E-4 1.7882193894840182E-4 10 0.0 4.4705484737100455E-5 0.0 1.3411645421130137E-4 1.7882193894840182E-4 11 0.0 4.4705484737100455E-5 0.0 1.3411645421130137E-4 1.7882193894840182E-4 12 0.0 4.4705484737100455E-5 0.0 1.3411645421130137E-4 2.2352742368550227E-4 13 4.4705484737100455E-5 4.4705484737100455E-5 0.0 1.3411645421130137E-4 2.2352742368550227E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTAC 25 0.0059222966 19.060955 1 TAGGACC 750 0.0 12.663253 4 GGCGAGG 1075 0.0 11.48168 19 GTCCTAA 1280 0.0 11.168529 1 CGACTGA 60 0.0058643646 11.086545 13 AGGACCT 1565 0.0 10.923082 5 TCCAACG 210 0.0 10.860045 18 CCTATAC 160 5.475158E-10 10.684619 3 GGACCTG 1560 0.0 10.5897455 6 GTCCTAC 2430 0.0 10.51098 1 ATTTAGA 735 0.0 10.373309 1 GACGTGA 1275 0.0 10.276134 7 CTTACAC 250 0.0 10.257234 3 TGTAGGA 2355 0.0 10.243981 2 GTAGGAC 2290 0.0 10.243963 3 TGGCGAG 2460 0.0 10.197847 18 TTAGGAC 1540 0.0 10.175828 3 GTGTAGG 310 0.0 10.145348 1 AGGACGT 2315 0.0 10.132883 5 GGACGTG 2285 0.0 10.096734 6 >>END_MODULE