FastQCFastQC Report
Thu 26 May 2016
SRR1512932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236862
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44510.19898411256483411No Hit
CCCATGTACTCTGCGTTGATACCAC43760.1956312012095516No Hit
GTCCTACAGTGGACATTTCTAAATT42320.18919361140740912No Hit
CTGTAGGACGTGGAATATGGCAAGA41360.18490188487264747No Hit
GTATCAACGCAGAGTACTTTTTTTT40680.18186191191052464No Hit
CTTTAGGACGTGAAATATGGCGAGG40170.17958193218893254No Hit
GTCCTAAAGTGTGTATTTCTCATTT38250.17099847911940924No Hit
TATCAACGCAGAGTACTTTTTTTTT30100.13456350905867237No Hit
GTCCTACAGTGTGCATTTCTCATTT26670.11922952779384692No Hit
GGTATCAACGCAGAGTACTTTTTTT26090.1166366096790951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCA604.084942E-412.6697259
GGCGAGG9650.012.48911719
TAGGACC7500.011.9250254
GTCGTAG600.005841101511.0924661
GTCCTAA11150.010.5737291
CGTGCGC909.505937E-510.55692410
TGGCGAG23200.010.55350518
TAGACTG2800.010.5340745
GTCCTAT2800.010.5265241
AGGACGT24600.010.4815845
GGACGTG24250.010.4331786
AGGACCT16500.010.3796165
TGTAGGA25100.010.3016882
CCTATAC1951.4551915E-1110.2380523
CCCCTAT1606.0917955E-910.1020681
CTAGACT1803.8380676E-1010.0432394
CTGTAGG26550.09.9912771
TAATACC2002.3646862E-119.990384
TGGACTG4000.09.990385
CTCGAAC2002.5465852E-119.96447218
Produced by FastQC (version 0.11.5)