##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512932_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236862 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.610431488397584 31.0 31.0 34.0 27.0 34.0 2 30.740139981813808 31.0 31.0 34.0 27.0 34.0 3 30.83815183949658 31.0 31.0 34.0 27.0 34.0 4 34.323863519519755 37.0 35.0 37.0 31.0 37.0 5 34.01894573737673 37.0 35.0 37.0 30.0 37.0 6 33.9028438052951 37.0 35.0 37.0 30.0 37.0 7 33.78468765618979 37.0 35.0 37.0 29.0 37.0 8 33.67450517734219 37.0 35.0 37.0 28.0 37.0 9 35.13988435585208 39.0 35.0 39.0 28.0 39.0 10 35.03220404298522 39.0 35.0 39.0 28.0 39.0 11 35.070944921948694 39.0 35.0 39.0 28.0 39.0 12 34.929920576235816 39.0 35.0 39.0 27.0 39.0 13 34.91832799698864 39.0 35.0 39.0 27.0 39.0 14 35.99438499111702 40.0 36.0 41.0 27.0 41.0 15 36.01666978114877 40.0 36.0 41.0 27.0 41.0 16 35.99381991378994 40.0 36.0 41.0 27.0 41.0 17 35.97504852780368 40.0 36.0 41.0 27.0 41.0 18 35.90467270667569 40.0 36.0 41.0 27.0 41.0 19 35.88029972345187 40.0 36.0 41.0 27.0 41.0 20 35.86182607599396 40.0 36.0 41.0 27.0 41.0 21 35.80869405443876 40.0 36.0 41.0 27.0 41.0 22 35.76854092921244 40.0 36.0 41.0 27.0 41.0 23 35.6640950581663 40.0 36.0 41.0 27.0 41.0 24 35.658401814685035 40.0 36.0 41.0 27.0 41.0 25 35.564208699508505 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4380337284364728 1101 2 0.4436291660525278 1101 3 0.4128638466614447 1101 4 0.229270690997744 1101 5 0.4671385428503001 1101 6 0.49290599899442356 1101 7 0.5378666792189009 1101 8 0.47498546983431567 1101 9 0.3337040010686039 1101 10 0.6909674704954014 1101 11 0.6445738759553805 1101 12 0.6771187714910809 1101 13 0.6401040371364246 1101 14 0.790324595778678 1101 15 0.732787196270472 1101 16 0.9639669928672134 1101 17 0.8201844771962783 1101 18 0.2326088062207674 1101 19 0.6352562589693562 1101 20 0.4434666660686162 1101 21 0.7386101245568852 1101 22 0.5884107876293925 1101 23 0.5795311310849414 1101 24 0.5679680137464302 1101 25 0.5169135023976423 1102 1 0.6027170264207271 1102 2 0.46674987142530355 1102 3 0.4983822008365131 1102 4 0.4884484516885905 1102 5 0.6280246545237773 1102 6 0.5025245503535842 1102 7 0.5456314443389445 1102 8 0.6719795610431945 1102 9 0.8281716721843253 1102 10 0.8874613229864892 1102 11 0.7976006441541017 1102 12 0.8221560247606376 1102 13 0.8354419132599844 1102 14 1.0566865249280468 1102 15 0.9960118839809908 1102 16 0.83018195533122 1102 17 0.9119131120385049 1102 18 0.989145525257257 1102 19 1.0350165819359276 1102 20 1.0482287703730577 1102 21 1.053680074674375 1102 22 0.8991259262077165 1102 23 0.982668423010459 1102 24 0.9022797786710868 1102 25 1.0538553533700892 1103 1 0.5390771103655041 1103 2 0.5161509298666331 1103 3 0.5194083772274993 1103 4 0.5363781730271313 1103 5 0.5337012104514471 1103 6 0.6282110440795279 1103 7 0.6321252525476666 1103 8 0.7032194576835096 1103 9 0.8005450043330242 1103 10 0.8547031973144712 1103 11 0.8331369932143247 1103 12 0.8233196027299883 1103 13 0.9164240276911002 1103 14 1.0567460859044218 1103 15 0.9012604070908949 1103 16 0.8911334830620419 1103 17 0.9925408222068981 1103 18 1.0242517025696216 1103 19 1.0385611181624128 1103 20 1.0406536671902487 1103 21 0.7562251546827028 1103 22 1.0145748423871623 1103 23 0.9743701146573613 1103 24 1.0342802294917348 1103 25 1.0528066194933032 1104 1 0.29860792119675494 1104 2 0.3183471152490398 1104 3 0.29614922957165746 1104 4 0.298451377288643 1104 5 0.3123448919285394 1104 6 0.37520858108708666 1104 7 0.36608343715825953 1104 8 0.3571199080745373 1104 9 0.44181099806395707 1104 10 0.5359147939689421 1104 11 0.5239402460247007 1104 12 0.3891533398494147 1104 13 0.5239542993189303 1104 14 0.56138639982602 1104 15 0.6334644544865426 1104 16 0.651651052586196 1104 17 0.6775886539063904 1104 18 0.727237829178037 1104 19 0.8058424849444421 1104 20 0.7719487129711666 1104 21 0.4671884506293438 1104 22 0.5520904544220855 1104 23 0.5810764571608544 1104 24 0.573148946199936 1104 25 0.695946098512664 1105 1 0.3533506657158263 1105 2 0.3862595238194153 1105 3 0.3886851620255527 1105 4 0.22710331986136367 1105 5 0.1958768320446822 1105 6 0.3696234294643119 1105 7 0.41515707015328474 1105 8 0.46569387864654743 1105 9 0.5398494959817342 1105 10 0.5270790446672322 1105 11 0.48141572775372055 1105 12 0.4343697466969587 1105 13 0.4287653471166806 1105 14 0.5493485378701592 1105 15 0.5493990982892925 1105 16 0.6056261579502191 1105 17 0.4374053966743432 1105 18 0.6278747203828914 1105 19 0.6162507652169751 1105 20 0.6993982269289916 1105 21 0.6651704230890445 1105 22 0.717096226160244 1105 23 0.7354573821823962 1105 24 0.6325900512382958 1105 25 0.6833046788064934 1106 1 0.5826336733476261 1106 2 0.5795222540249583 1106 3 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0.6646354617116614 1107 16 0.5917003418740876 1107 17 0.6099021453164184 1107 18 0.7798499570994082 1107 19 0.9785973923377114 1107 20 0.8534166699257781 1107 21 0.833338304969125 1107 22 0.7932904013745983 1107 23 0.7407572427123839 1107 24 0.7558944901510145 1107 25 0.7483800926558004 1108 1 0.3595540403066444 1108 2 0.24788756330047335 1108 3 0.268120029675341 1108 4 0.2373518169992579 1108 5 0.4054906226289816 1108 6 0.2739315040993091 1108 7 0.1743002163059444 1108 8 0.39420181872250737 1108 9 0.3018944031094577 1108 10 0.40666064723103545 1108 11 0.3276512747549418 1108 12 0.49256153547360526 1108 13 0.4405717319586486 1108 14 0.4701524266575845 1108 15 0.5667224036961613 1108 16 0.4781910998572414 1108 17 0.4355800299541315 1108 18 0.4823672283167255 1108 19 0.3819335274548976 1108 20 0.3884904373343758 1108 21 0.4342992703926356 1108 22 0.56497497004694 1108 23 0.5063785808505443 1108 24 0.5391871124813932 1108 25 0.4619993936958693 1109 1 0.17338668969383875 1109 2 0.09826284935309104 1109 3 0.24126822521860092 1109 4 0.30541267676137807 1109 5 0.22281863836952454 1109 6 -0.05733459318791745 1109 7 0.20643798598817398 1109 8 0.3798851427132277 1109 9 0.5087230861500487 1109 10 0.32641797223799074 1109 11 0.30831268209012563 1109 12 0.4362333586821663 1109 13 0.48912639375799927 1109 14 0.5967222047693923 1109 15 0.6591958958414992 1109 16 0.4257618530136469 1109 17 0.4125403877118572 1109 18 0.5520763628991006 1109 19 0.7038493090698807 1109 20 0.6491840923034147 1109 21 0.7389975870986021 1109 22 0.7138637188312558 1109 23 0.6270771797077046 1109 24 0.35719678552309375 1109 25 0.5195654152017823 1110 1 0.24476862593683535 1110 2 0.2381938292784369 1110 3 0.2614122858299943 1110 4 0.2778165006594264 1110 5 0.1720438392127619 1110 6 0.18177008611485235 1110 7 0.28164119997910575 1110 8 0.3032066035705512 1110 9 0.4544470607530542 1110 10 0.46465823172302834 1110 11 0.3651439725215866 1110 12 0.4005309267274271 1110 13 0.18551464680707141 1110 14 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2216 12 -1.0467260934530245 2216 13 -1.5488536897321552 2216 14 -1.501803820751988 2216 15 -1.5733436601505773 2216 16 -1.3986319522871895 2216 17 -1.6356337758958972 2216 18 -1.3673084348171471 2216 19 -1.3687352747957888 2216 20 -1.943065097214955 2216 21 -1.6699370390749095 2216 22 -1.9533996570466456 2216 23 -1.0144532789254015 2216 24 -1.391294636670338 2216 25 -1.1857859073956973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49931.0 3 2.0 4 749.0 5 6855.0 6 8126.0 7 6355.0 8 5199.0 9 4657.0 10 4092.0 11 3710.0 12 3472.0 13 3200.0 14 3178.0 15 3084.0 16 3243.0 17 3497.0 18 3906.0 19 4619.0 20 5978.0 21 7808.0 22 9958.0 23 12280.0 24 15002.0 25 17437.0 26 19935.0 27 22094.0 28 24995.0 29 30337.0 30 38623.0 31 49501.0 32 62779.0 33 82061.0 34 106458.0 35 139138.0 36 192192.0 37 300001.0 38 982410.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.40213087514059 19.699410058797266 13.487489363072886 27.410969702989256 2 13.397759983977533 20.80166876857236 40.38926905987369 25.411302187576418 3 16.935376433593134 25.126807107456784 30.241069855896342 27.69674660305374 4 11.523196518275075 17.59613582913002 37.76134961560873 33.11931803698617 5 12.486337392035757 38.32621587703541 36.27562787597059 12.911818854958245 6 31.57165457763556 37.6650273546766 18.04648057970081 12.716837487987029 7 27.38240908204575 31.671013769403196 22.874078924065238 18.072498224485816 8 24.817099277076306 37.51208489152565 20.517897718757226 17.15291811264081 9 26.18949534256992 14.924709539624498 20.3222293043488 38.56356581345678 10 14.636017417777358 29.16121559221383 34.88689141728234 21.31587557272647 11 33.718313775826125 22.282995273919106 25.18731527220232 18.81137567805245 12 23.146837044704117 24.854566875093614 32.09038739691361 19.90820868328866 13 28.79837764686369 22.088198462859747 25.815345561486282 23.298078328790286 14 20.55560448679058 22.416337647577972 27.71047870097854 29.317579164652912 15 22.737462187834474 31.357920592440518 23.257019001982375 22.647598217742633 16 21.764037809752914 28.011142609302205 28.2677982168248 21.957021364120084 17 21.568139050671856 28.83482277013781 28.124731686724573 21.472306492465762 18 21.535257113810687 27.402449290530136 30.075719405886325 20.98657418977285 19 23.27566088600974 27.14271024736562 27.759045409228484 21.822583457396156 20 23.564999526092624 27.388984961935403 28.047805630019617 20.998209881952356 21 22.4537576195149 26.898167292917957 27.45694289527037 23.191132192296774 22 23.057991896629495 28.176491284383776 27.140345316420838 21.625171502565887 23 21.689769726415722 28.13178825148277 27.93530755822557 22.24313446387594 24 22.94989404790644 27.270772422055916 28.28540901084556 21.49392451919208 25 22.405024356397398 27.37546405911344 27.948116581663673 22.271395002825486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 148.0 1 148.0 2 342.5 3 537.0 4 537.0 5 537.0 6 1297.0 7 2057.0 8 2057.0 9 2057.0 10 3021.5 11 3986.0 12 3986.0 13 3986.0 14 6913.5 15 9841.0 16 9841.0 17 9841.0 18 18384.0 19 26927.0 20 26927.0 21 26927.0 22 43025.0 23 59123.0 24 59123.0 25 59123.0 26 84765.0 27 110407.0 28 110407.0 29 110407.0 30 142018.5 31 173630.0 32 173630.0 33 173630.0 34 196135.5 35 218641.0 36 218641.0 37 218641.0 38 235372.0 39 252103.0 40 252103.0 41 252103.0 42 263881.0 43 275659.0 44 275659.0 45 275659.0 46 288410.0 47 301161.0 48 301161.0 49 301161.0 50 287017.0 51 272873.0 52 272873.0 53 272873.0 54 239457.0 55 206041.0 56 206041.0 57 206041.0 58 176266.5 59 146492.0 60 146492.0 61 146492.0 62 119034.0 63 91576.0 64 91576.0 65 91576.0 66 70662.0 67 49748.0 68 49748.0 69 49748.0 70 36125.5 71 22503.0 72 22503.0 73 22503.0 74 15714.0 75 8925.0 76 8925.0 77 8925.0 78 6049.0 79 3173.0 80 3173.0 81 3173.0 82 2066.0 83 959.0 84 959.0 85 959.0 86 622.5 87 286.0 88 286.0 89 286.0 90 174.5 91 63.0 92 63.0 93 63.0 94 32.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07707225568676118 2 1.3411645421130137E-4 3 0.0 4 0.03259029837334623 5 0.0781004818357145 6 0.07948635186256461 7 0.03978788141601941 8 0.07300405657568505 9 0.053065410382938244 10 0.11592132192330148 11 0.005588185592137557 12 0.011936364424805822 13 0.00496230880581815 14 0.007286994012147375 15 0.0066164117410908675 16 0.017971604864314383 17 0.029952674773857303 18 0.002279979721592123 19 0.004112904595813242 20 0.006169356893719863 21 3.129383931597032E-4 22 6.705822710565069E-4 23 4.4705484737100455E-5 24 8.941096947420091E-5 25 0.003487027809493836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2236862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.76114038437952 #Duplication Level Percentage of deduplicated Percentage of total 1 81.1405223137458 55.793148456773544 2 12.656291676896572 17.40522097481478 3 3.3874670346619515 6.987782889535447 4 1.2233114505066762 3.3646516152843398 5 0.5457932094635903 1.8764681748383496 6 0.2864363023151281 1.1817412076803857 7 0.17917877282349848 0.8624375726412198 8 0.1078243547648311 0.5931300475871754 9 0.08095504977277962 0.5009905388025477 >10 0.3505688002101773 4.162573050519345 >50 0.021099948643378014 1.0036323731734231 >100 0.016509646922695183 2.3717013780276694 >500 0.002216273149649781 1.1123378601383425 >1k 0.001825166123239508 2.784183860183505 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4451 0.19898411256483411 No Hit CCCATGTACTCTGCGTTGATACCAC 4376 0.1956312012095516 No Hit GTCCTACAGTGGACATTTCTAAATT 4232 0.18919361140740912 No Hit CTGTAGGACGTGGAATATGGCAAGA 4136 0.18490188487264747 No Hit GTATCAACGCAGAGTACTTTTTTTT 4068 0.18186191191052464 No Hit CTTTAGGACGTGAAATATGGCGAGG 4017 0.17958193218893254 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3825 0.17099847911940924 No Hit TATCAACGCAGAGTACTTTTTTTTT 3010 0.13456350905867237 No Hit GTCCTACAGTGTGCATTTCTCATTT 2667 0.11922952779384692 No Hit GGTATCAACGCAGAGTACTTTTTTT 2609 0.1166366096790951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.4705484737100455E-5 0.0 4 0.0 0.0 0.0 4.4705484737100455E-5 0.0 5 0.0 0.0 0.0 4.4705484737100455E-5 0.0 6 0.0 0.0 0.0 4.4705484737100455E-5 0.0 7 0.0 0.0 0.0 4.4705484737100455E-5 0.0 8 0.0 0.0 0.0 4.4705484737100455E-5 0.0 9 0.0 0.0 0.0 8.941096947420091E-5 0.0 10 0.0 0.0 0.0 1.3411645421130137E-4 0.0 11 0.0 0.0 0.0 1.3411645421130137E-4 0.0 12 0.0 0.0 0.0 1.3411645421130137E-4 4.4705484737100455E-5 13 0.0 0.0 0.0 1.3411645421130137E-4 8.941096947420091E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCCA 60 4.084942E-4 12.669725 9 GGCGAGG 965 0.0 12.489117 19 TAGGACC 750 0.0 11.925025 4 GTCGTAG 60 0.0058411015 11.092466 1 GTCCTAA 1115 0.0 10.573729 1 CGTGCGC 90 9.505937E-5 10.556924 10 TGGCGAG 2320 0.0 10.553505 18 TAGACTG 280 0.0 10.534074 5 GTCCTAT 280 0.0 10.526524 1 AGGACGT 2460 0.0 10.481584 5 GGACGTG 2425 0.0 10.433178 6 AGGACCT 1650 0.0 10.379616 5 TGTAGGA 2510 0.0 10.301688 2 CCTATAC 195 1.4551915E-11 10.238052 3 CCCCTAT 160 6.0917955E-9 10.102068 1 CTAGACT 180 3.8380676E-10 10.043239 4 CTGTAGG 2655 0.0 9.991277 1 TAATACC 200 2.3646862E-11 9.99038 4 TGGACTG 400 0.0 9.99038 5 CTCGAAC 200 2.5465852E-11 9.964472 18 >>END_MODULE