FastQCFastQC Report
Thu 26 May 2016
SRR1512931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75115
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA2030.2702522798375824No Hit
GTATCAACGCAGAGTACTTTTTTTT1760.23430739532716502No Hit
GGTATCAACGCAGAGTACTTTTTTT1270.16907408640085203No Hit
TATCAACGCAGAGTACTTTTTTTTT1060.14111695400386073No Hit
GTCCTACAGTGGACATTTCTAAATT880.11715369766358251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG751.9770919E-411.4288391
TGTAGGA752.026832E-411.3984022
ATGGCAA700.001464697110.85562117
GTATCAA856.2904233E-410.0842691
TAGGACG856.447057E-410.0574134
TGGAATA856.447057E-410.05741311
GTAGGAC856.447057E-410.0574133
ATATGGC1304.049107E-69.49866915
GGAATAT900.00108916459.49866912
TATGGCA800.00443670959.49866916
AATATGG1401.0372876E-58.82019214
GAATATG1000.00284240848.54880213
Produced by FastQC (version 0.11.5)