FastQCFastQC Report
Thu 26 May 2016
SRR1512931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75115
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3340.44465153431405174No Hit
GGTATCAACGCAGAGTACTTTTTTT2190.2915529521400519No Hit
TATCAACGCAGAGTACTTTTTTTTT2060.2742461558942954No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1420.1890434666844172No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1080.14377953804166943No Hit
GTACTTTTTTTTTTTTTTTTTTTTT840.11182852958796512No Hit
CTGTAGGACGTGGAATATGGCAAGA830.11049723756906078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTGC550.003017173412.0934528
GTATCAA1303.6232632E-710.2329221
ATTTCTC1106.637808E-59.49567215
CATTTCT950.00178918918.995914
TATCAAC1701.3736908E-57.82517532
TCAACGC1752.0034546E-57.60666664
CAACGCA1752.0034546E-57.60666665
ATCAACG1752.021367E-57.60159873
AGAGTAC2001.534093E-57.12175411
AACGCAG1905.947551E-57.006146
CGCAGAG1906.0001446E-57.0014738
GAGTACT1500.0018711126.96349312
CAGAGTA2052.1488531E-56.952682510
ACGCAGA2051.6217632E-46.48917067
GCAGAGT2102.217779E-46.3346669
AGTACTT1650.00508807466.33044813
GTACTTT1750.0093698025.968708514
Produced by FastQC (version 0.11.5)