FastQCFastQC Report
Thu 26 May 2016
SRR1512930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932829
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29350.1518499567214689No Hit
GTCCTACAGTGGACATTTCTAAATT19370.10021579767273774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3950.012.0219424
TGTACCG802.8754508E-511.8716685
GACCGAG658.0631167E-411.6856997
CGCTTTC600.005866169511.08596212
GGTATCA8200.011.04131
GTAGGAC10700.011.0062563
TGTAGGA11800.010.9460792
AGGACGT11300.010.842095
GTGTACG803.6618827E-410.72168351
GGACGTG11700.010.7118926
GACGTGG5600.010.681467
GGCGAGG5900.010.62166219
TAGGACG12250.010.1563334
GTCCTAC11350.010.0761791
CTGTAGG12250.010.0360791
GTATCAA16950.010.008311
ACCGAGT951.6528352E-49.9948668
ATAGGAC2201.8189894E-129.9290313
ACGTGGA6350.09.8689468
GTACTAA1451.543649E-79.8590191
Produced by FastQC (version 0.11.5)