##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1932829 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.869873641175708 31.0 30.0 34.0 23.0 34.0 2 29.037150208321584 31.0 30.0 34.0 22.0 34.0 3 29.261696197646042 31.0 30.0 34.0 23.0 34.0 4 32.703654591275274 35.0 33.0 37.0 25.0 37.0 5 32.84995775622158 37.0 35.0 37.0 26.0 37.0 6 32.84742054263466 37.0 35.0 37.0 26.0 37.0 7 32.695542130214314 37.0 33.0 37.0 25.0 37.0 8 32.63904566829243 37.0 33.0 37.0 25.0 37.0 9 33.9907079208766 39.0 35.0 39.0 24.0 39.0 10 33.89107003257919 38.0 34.0 39.0 23.0 39.0 11 33.923317065296516 38.0 34.0 39.0 23.0 39.0 12 33.83479552510853 38.0 34.0 39.0 23.0 39.0 13 33.823462913687656 38.0 34.0 39.0 23.0 39.0 14 34.82195993541074 39.0 34.0 41.0 22.0 41.0 15 34.74990958848403 39.0 34.0 41.0 20.0 41.0 16 34.653044320009684 39.0 34.0 41.0 19.0 41.0 17 34.57943667029003 39.0 34.0 41.0 18.0 41.0 18 34.296065508123064 39.0 34.0 41.0 17.0 41.0 19 34.524644446042565 39.0 34.0 41.0 18.0 41.0 20 34.59332098183543 39.0 34.0 41.0 18.0 41.0 21 34.53496299983082 39.0 34.0 41.0 18.0 41.0 22 34.44035452696539 39.0 34.0 41.0 18.0 41.0 23 34.185990586854814 39.0 34.0 41.0 10.0 41.0 24 34.17496684911081 39.0 34.0 41.0 10.0 41.0 25 34.08830941588728 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5060817363892234 1101 2 0.8063870136458782 1101 3 0.8354534912335545 1101 4 0.7521252709912929 1101 5 0.5655660145320454 1101 6 0.7222983139914945 1101 7 0.8662053573761597 1101 8 0.6138003123480686 1101 9 0.48184449854946365 1101 10 0.6588560748446426 1101 11 0.7867926776372727 1101 12 1.0628666200038523 1101 13 0.8610144897898522 1101 14 0.7465393375507361 1101 15 0.48507645614014194 1101 16 0.9888405370938997 1101 17 1.0580437757325 1101 18 1.2759019422238893 1101 19 0.7222219485116526 1101 20 0.9755156532791247 1101 21 0.8443100523273728 1101 22 0.9317595071701206 1101 23 1.198760041508251 1101 24 0.7426183528420509 1101 25 1.0644994947213746 1102 1 0.4329621563435282 1102 2 0.5346652883863321 1102 3 0.6486211977903373 1102 4 0.7113535030244762 1102 5 0.5071847762941957 1102 6 0.46159557309711374 1102 7 0.634632099175235 1102 8 0.6992048886119164 1102 9 0.7596188337271599 1102 10 0.8910551250814507 1102 11 0.8456729717896323 1102 12 0.7990939794157654 1102 13 0.6674305137196299 1102 14 0.7526193498735196 1102 15 0.8876449748668307 1102 16 0.8654974354261 1102 17 0.7897806698837613 1102 18 1.1662187316668238 1102 19 1.1056483306541693 1102 20 0.9456709055467982 1102 21 0.7964321350222292 1102 22 1.1196276945456844 1102 23 1.1797854332614293 1102 24 1.0615192340888484 1102 25 1.033014339403195 1103 1 0.24164146365617256 1103 2 0.4787017400837996 1103 3 0.5712514738667878 1103 4 0.6618517409968874 1103 5 0.1114435239499656 1103 6 0.5953474299937085 1103 7 0.5038091128272058 1103 8 0.5015162092801617 1103 9 0.7870019095850935 1103 10 0.6105227342663611 1103 11 0.7557279866478623 1103 12 0.9450251225430009 1103 13 0.9501621545043193 1103 14 1.0972495807306046 1103 15 1.13967541605507 1103 16 1.1989138746210202 1103 17 1.0518849469635896 1103 18 1.3201356759131784 1103 19 1.3170067020725966 1103 20 1.246199727698425 1103 21 1.1118448503412637 1103 22 1.2279005775307965 1103 23 1.596478834350485 1103 24 1.3202513075128834 1103 25 1.2098245042277824 1104 1 0.6055488211701281 1104 2 0.6262081135700974 1104 3 0.48898099179946186 1104 4 0.7999083346581699 1104 5 0.6109443882333352 1104 6 0.55726826356846 1104 7 0.8155251015866938 1104 8 0.6808429373640621 1104 9 0.9053538081114638 1104 10 0.9364004164418418 1104 11 0.9274053240756785 1104 12 0.8603434588699415 1104 13 0.865216136001898 1104 14 1.1630618622455628 1104 15 1.1953396105815983 1104 16 1.1432110529048174 1104 17 1.0881057834353811 1104 18 1.3066741003101114 1104 19 1.1395449429903763 1104 20 1.1962783098874255 1104 21 1.2952690782803131 1104 22 1.0923638044462223 1104 23 1.4331093604528107 1104 24 1.3106476224789887 1104 25 0.9371382728895483 1105 1 0.6137477517365362 1105 2 0.7705291896605146 1105 3 0.6612345083402253 1105 4 0.8255054591987019 1105 5 0.5868468584946882 1105 6 0.35492607376208696 1105 7 0.5523769654980129 1105 8 0.7742989878401687 1105 9 0.635742400337449 1105 10 0.7645303909722472 1105 11 0.7061219314097613 1105 12 0.7479136449716464 1105 13 0.7632436894735761 1105 14 0.7825247576755032 1105 15 1.071077650001584 1105 16 1.0530219122142412 1105 17 0.9749587326987879 1105 18 1.0210383734136386 1105 19 0.9071945768649385 1105 20 0.9290347785858728 1105 21 0.8760310316246418 1105 22 0.7784456110509552 1105 23 0.9711826072473997 1105 24 1.0833387168033184 1105 25 1.1039387996470964 1106 1 0.7955362831189277 1106 2 0.7946676774528108 1106 3 0.8441427434859037 1106 4 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0.5177408334690767 1107 17 0.5377248186147625 1107 18 0.9526162807350502 1107 19 0.8507126545891239 1107 20 0.7372984248299517 1107 21 0.7540661002238878 1107 22 0.62848797720833 1107 23 0.7643071138488367 1107 24 0.7767395765019742 1107 25 0.8216819858815327 1108 1 0.39576092969253907 1108 2 0.47273240167729824 1108 3 0.27532273841130817 1108 4 0.4612310467032401 1108 5 0.5479098341497846 1108 6 0.6874202863084236 1108 7 0.7378307586599391 1108 8 0.6149262326282638 1108 9 0.5538694291459336 1108 10 0.7589681160510011 1108 11 0.6361320384556564 1108 12 0.6404973850665812 1108 13 0.6001724833954256 1108 14 0.7859712323282935 1108 15 0.7948872223779517 1108 16 0.7392880286928474 1108 17 0.8066210237785114 1108 18 0.9974417009395253 1108 19 0.9174972042969074 1108 20 0.9154946567733688 1108 21 0.7302707913913196 1108 22 0.6014401203692756 1108 23 0.7922963941059251 1108 24 0.8087822349352081 1108 25 0.8398494296836176 1109 1 0.3025829502913453 1109 2 0.2103865853451552 1109 3 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-1.987407126791382 2216 18 -2.017694079479753 2216 19 -2.320768533136352 2216 20 -2.067804536650236 2216 21 -2.4148529755917423 2216 22 -3.1113779301690023 2216 23 -2.7740792424436265 2216 24 -2.7807786888268424 2216 25 -2.9773631672143033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95475.0 3 3.0 4 809.0 5 4601.0 6 5627.0 7 5127.0 8 4609.0 9 4369.0 10 4073.0 11 3826.0 12 3784.0 13 3631.0 14 3607.0 15 3710.0 16 3876.0 17 4090.0 18 4501.0 19 5502.0 20 7085.0 21 8740.0 22 11056.0 23 13446.0 24 15544.0 25 17655.0 26 19572.0 27 22013.0 28 24852.0 29 29148.0 30 36421.0 31 45397.0 32 57390.0 33 73622.0 34 95311.0 35 125324.0 36 171347.0 37 268409.0 38 729277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32057927549543 18.569382057330433 12.51602478396073 25.5940138832134 2 15.129161986884535 20.243987019103862 40.731029414048145 23.895821579963457 3 18.42617099879202 24.740160463399917 29.815946347961912 27.01772218984615 4 12.308937627739361 16.83041284947341 38.328821105117235 32.53182841766999 5 13.257390290787772 37.659712783544215 35.985749939701726 13.097146985966287 6 31.668177669040805 37.159688112551045 18.255289025776044 12.916845192632104 7 27.676265474150803 31.661937896928173 22.7273315301395 17.934465098781523 8 24.976570379681462 36.31552337191954 20.97348196501418 17.734424283384815 9 26.366965650109165 15.152887261007733 20.168936943351838 38.31121014553126 10 14.806519819958746 28.724631891395667 34.83600576678469 21.632842521860894 11 34.09802082001917 22.475933612320418 24.794627799595027 18.63141776806538 12 22.805840053652155 24.775724883670865 31.876200549768996 20.54223451290798 13 28.681969167652895 21.944825509159095 25.835635677178875 23.537569646009135 14 21.14831471140759 21.6300179577811 27.813860094912307 29.407807235899003 15 23.13148621492534 30.17045745653689 23.487285168591775 23.210771159945992 16 22.652630445931003 27.971118214433748 26.980240929938436 22.396010409696814 17 21.952379449193 28.198495032863264 27.55344258256956 22.29568293537418 18 21.833599840923966 27.670685683985518 28.859406198994176 21.63630827609634 19 23.429816689902573 27.09845090495841 27.436018452105564 22.03571395303345 20 23.477062990549065 27.268719070213688 27.704549935546503 21.549668003690744 21 23.327238788011773 26.71517117426691 27.09745597152452 22.860134066196803 22 23.210798290670827 27.516670513243785 27.164631085655856 22.107900110429537 23 22.712124379788076 27.388268705715785 27.2776810646221 22.621925849874042 24 22.961069014748475 26.948813203406413 27.81935666822426 22.27076111362085 25 22.972779962740635 27.061633202235562 27.494514593251917 22.47107224177189 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 248.0 2 414.5 3 581.0 4 581.0 5 581.0 6 1070.0 7 1559.0 8 1559.0 9 1559.0 10 2452.0 11 3345.0 12 3345.0 13 3345.0 14 5968.0 15 8591.0 16 8591.0 17 8591.0 18 15688.5 19 22786.0 20 22786.0 21 22786.0 22 35326.5 23 47867.0 24 47867.0 25 47867.0 26 67788.5 27 87710.0 28 87710.0 29 87710.0 30 112436.0 31 137162.0 32 137162.0 33 137162.0 34 158022.0 35 178882.0 36 178882.0 37 178882.0 38 196110.5 39 213339.0 40 213339.0 41 213339.0 42 226288.5 43 239238.0 44 239238.0 45 239238.0 46 244945.0 47 250652.0 48 250652.0 49 250652.0 50 243575.0 51 236498.0 52 236498.0 53 236498.0 54 214173.5 55 191849.0 56 191849.0 57 191849.0 58 165534.0 59 139219.0 60 139219.0 61 139219.0 62 113936.5 63 88654.0 64 88654.0 65 88654.0 66 68619.5 67 48585.0 68 48585.0 69 48585.0 70 35570.5 71 22556.0 72 22556.0 73 22556.0 74 15708.0 75 8860.0 76 8860.0 77 8860.0 78 6045.5 79 3231.0 80 3231.0 81 3231.0 82 2115.0 83 999.0 84 999.0 85 999.0 86 645.5 87 292.0 88 292.0 89 292.0 90 189.5 91 87.0 92 87.0 93 87.0 94 56.0 95 25.0 96 25.0 97 25.0 98 19.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35730010259572886 2 0.039268864446880714 3 0.034922903164221974 4 0.03476769026126988 5 0.03968276552141964 6 0.017435582764952304 7 0.017176894593365476 8 0.023074984905545187 9 0.02028115265240743 10 0.019815513943551136 11 0.01872902362288645 12 0.019712038674916405 13 0.019556825771964305 14 0.026851832210712898 15 0.028869599949090167 16 0.02069505372694636 17 0.0221437074878326 18 0.08676401275022261 19 0.0245753763007488 20 0.0221437074878326 21 0.025868817158682948 22 0.019401612869012208 23 0.021988494584880502 24 0.01852207308561699 25 0.02219544512214997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1932829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.49272783591367 #Duplication Level Percentage of deduplicated Percentage of total 1 84.25650280473273 63.60753234643586 2 10.888922640256975 16.440689466144768 3 2.6931696174460273 6.099441628374141 4 0.9651916418470904 2.914597997098442 5 0.4103111116560888 1.5487752540152147 6 0.22144953500908499 1.0030697685498293 7 0.135817780018354 0.7177278291542515 8 0.07907889015813169 0.47759049058191577 9 0.06407291349427507 0.4353335118069613 >10 0.2614975727860736 3.317711538133349 >50 0.013148427567541478 0.6895623715980145 >100 0.009260312973176012 1.440205709247502 >500 0.0010283165573205689 0.5427401569461671 >1k 5.484354972376263E-4 0.7650219319136193 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2935 0.1518499567214689 No Hit GTCCTACAGTGGACATTTCTAAATT 1937 0.10021579767273774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1737634317365895E-5 2 0.0 0.0 0.0 0.0 5.1737634317365895E-5 3 0.0 0.0 0.0 0.0 5.1737634317365895E-5 4 0.0 0.0 0.0 0.0 5.1737634317365895E-5 5 0.0 5.1737634317365895E-5 0.0 0.0 5.1737634317365895E-5 6 0.0 5.1737634317365895E-5 0.0 0.0 1.0347526863473179E-4 7 0.0 5.1737634317365895E-5 0.0 5.1737634317365895E-5 1.5521290295209767E-4 8 0.0 5.1737634317365895E-5 0.0 5.1737634317365895E-5 1.5521290295209767E-4 9 0.0 5.1737634317365895E-5 0.0 1.0347526863473179E-4 1.5521290295209767E-4 10 0.0 5.1737634317365895E-5 0.0 1.0347526863473179E-4 1.5521290295209767E-4 11 0.0 5.1737634317365895E-5 0.0 1.0347526863473179E-4 1.5521290295209767E-4 12 0.0 5.1737634317365895E-5 0.0 1.0347526863473179E-4 2.0695053726946358E-4 13 0.0 5.1737634317365895E-5 0.0 1.0347526863473179E-4 2.0695053726946358E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 395 0.0 12.021942 4 TGTACCG 80 2.8754508E-5 11.871668 5 GACCGAG 65 8.0631167E-4 11.685699 7 CGCTTTC 60 0.0058661695 11.085962 12 GGTATCA 820 0.0 11.0413 1 GTAGGAC 1070 0.0 11.006256 3 TGTAGGA 1180 0.0 10.946079 2 AGGACGT 1130 0.0 10.84209 5 GTGTACG 80 3.6618827E-4 10.7216835 1 GGACGTG 1170 0.0 10.711892 6 GACGTGG 560 0.0 10.68146 7 GGCGAGG 590 0.0 10.621662 19 TAGGACG 1225 0.0 10.156333 4 GTCCTAC 1135 0.0 10.076179 1 CTGTAGG 1225 0.0 10.036079 1 GTATCAA 1695 0.0 10.00831 1 ACCGAGT 95 1.6528352E-4 9.994866 8 ATAGGAC 220 1.8189894E-12 9.929031 3 ACGTGGA 635 0.0 9.868946 8 GTACTAA 145 1.543649E-7 9.859019 1 >>END_MODULE