FastQCFastQC Report
Thu 26 May 2016
SRR1512930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932829
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35150.1818577846255411No Hit
TATCAACGCAGAGTACTTTTTTTTT25000.12934408579341472No Hit
GGTATCAACGCAGAGTACTTTTTTT22250.11511623635613911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG456.681403E-414.8015645
TTCGCAG400.005273322614.25260310
GGTATCA7300.012.6331211
GGCGAGG4550.012.09643619
TAGGACA4550.011.7111274
GTACCGT752.0474958E-411.4159816
GTATCAA15200.010.1954361
TAGGACC4300.010.1791494
CGGATCA750.002641218710.13649759
TGTAGGA11500.010.0853272
TTATACT3050.09.9832564
CTTAGAC2002.5465852E-119.9825113
AGGACGT9450.09.96845
CGAAATC1054.1454434E-59.94363913
GTCCTAC10250.09.9247971
TATGGCG10000.09.87132116
TAAGGGT1356.303071E-79.8677094
TGGCGAG9300.09.79555718
GTAGGAC10350.09.7367943
GGACGTG9650.09.562616
Produced by FastQC (version 0.11.5)