##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512930_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1932829 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.410126296739133 31.0 31.0 34.0 27.0 34.0 2 30.577778479110155 31.0 31.0 34.0 27.0 34.0 3 30.664061331861227 31.0 31.0 34.0 27.0 34.0 4 34.13352034763551 37.0 35.0 37.0 30.0 37.0 5 33.7893305615758 37.0 35.0 37.0 28.0 37.0 6 33.66442090841973 37.0 35.0 37.0 28.0 37.0 7 33.53458841935836 37.0 35.0 37.0 28.0 37.0 8 33.41278716327207 37.0 35.0 37.0 27.0 37.0 9 34.87250915626783 39.0 35.0 39.0 27.0 39.0 10 34.75787149302913 39.0 35.0 39.0 27.0 39.0 11 34.785713066184336 39.0 35.0 39.0 27.0 39.0 12 34.63784483779993 39.0 35.0 39.0 27.0 39.0 13 34.61890110299463 39.0 35.0 39.0 27.0 39.0 14 35.67824623906202 40.0 36.0 41.0 27.0 41.0 15 35.68189425965773 40.0 36.0 41.0 27.0 41.0 16 35.65508847394157 40.0 36.0 41.0 27.0 41.0 17 35.62605124405729 40.0 36.0 41.0 27.0 41.0 18 35.55138504233949 40.0 36.0 41.0 27.0 41.0 19 35.53750797406289 40.0 36.0 41.0 26.0 41.0 20 35.51747205779714 40.0 36.0 41.0 26.0 41.0 21 35.46127256989625 40.0 36.0 41.0 26.0 41.0 22 35.42487566153033 40.0 36.0 41.0 26.0 41.0 23 35.31377012658647 40.0 36.0 41.0 25.0 41.0 24 35.2906325391434 40.0 36.0 41.0 25.0 41.0 25 35.18280665283892 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5848484900244593 1101 2 0.6003061342509888 1101 3 0.6301334881676546 1101 4 0.39534823282619413 1101 5 0.6137773244409743 1101 6 0.6628152570996164 1101 7 0.7231890613683447 1101 8 0.5871552847471477 1101 9 0.46616167466694947 1101 10 0.7474135458158173 1101 11 0.8963011988138518 1101 12 0.8017984276406551 1101 13 0.9264270865382898 1101 14 0.9825460099093135 1101 15 0.8807121118223264 1101 16 1.0624698124728198 1101 17 1.0208141356274112 1101 18 0.62978772919449 1101 19 0.8764662035842292 1101 20 0.877293264854039 1101 21 1.0391907682160948 1101 22 0.9258585671721633 1101 23 0.8461844699884011 1101 24 0.8243785191910931 1101 25 0.8309783551242091 1102 1 0.7142841634504897 1102 2 0.6910464984393272 1102 3 0.5751273127659005 1102 4 0.5833317386545573 1102 5 0.7658344094624354 1102 6 0.5936536637701977 1102 7 0.6339123384966427 1102 8 0.5957566296862282 1102 9 0.9031179276621373 1102 10 0.9169014664933286 1102 11 0.8543486363205375 1102 12 0.8912935393734429 1102 13 0.9070012727880794 1102 14 0.9525368477492506 1102 15 0.930575250304841 1102 16 0.9939890235825075 1102 17 0.9892744868909489 1102 18 0.98984899432822 1102 19 1.0668318502690397 1102 20 1.0670604181106071 1102 21 1.0808362992212466 1102 22 1.164826322741888 1102 23 1.1477441961760988 1102 24 0.9473431770435283 1102 25 1.1297029704032724 1103 1 0.6442955843957314 1103 2 0.5922861812438072 1103 3 0.6975984655603042 1103 4 0.6767683921281673 1103 5 0.7108736311515997 1103 6 0.7247528548935094 1103 7 0.7210063648350911 1103 8 0.8050068009392959 1103 9 0.9020885760161832 1103 10 0.9993595912217828 1103 11 0.8890615269562829 1103 12 0.9741934695278545 1103 13 1.0239782726298117 1103 14 1.1090850702309751 1103 15 0.8491269229019665 1103 16 0.9165468875663194 1103 17 1.204793956577781 1103 18 1.1759462391855848 1103 19 1.3378721462236811 1103 20 1.2751269419085176 1103 21 0.8603967792064822 1103 22 1.067892195747575 1103 23 1.1633115739478512 1103 24 1.1847075724147729 1103 25 1.2540366722133243 1104 1 0.8030768724193713 1104 2 0.8604669130359177 1104 3 0.7517990517279074 1104 4 0.7837373406960637 1104 5 0.8313783919527253 1104 6 0.9790766859381819 1104 7 0.933461340017125 1104 8 0.8797582591157109 1104 9 1.142386512358577 1104 10 1.0962782278751249 1104 11 1.0838391309117057 1104 12 1.034056560282437 1104 13 1.1216435004336631 1104 14 1.2449640560741102 1104 15 1.1411599443207407 1104 16 1.333406690181377 1104 17 1.2710401904267883 1104 18 1.3303004891421324 1104 19 1.3526923125137316 1104 20 1.2761161600941975 1104 21 1.0346862803363237 1104 22 1.2443369733515297 1104 23 1.1661633469358534 1104 24 1.3460219591115674 1104 25 1.3408844762575924 1105 1 0.59367537173922 1105 2 0.6224229707477065 1105 3 0.5845996580779413 1105 4 0.4793114557723257 1105 5 0.5222954421104617 1105 6 0.6684235934082992 1105 7 0.6804160229496006 1105 8 0.7956407588986423 1105 9 0.9259428855460357 1105 10 0.9076702642850663 1105 11 0.8919922957983957 1105 12 0.8457199347144382 1105 13 0.8722241638008583 1105 14 0.9097684394494152 1105 15 0.9404086997836814 1105 16 1.0217876342929841 1105 17 0.8172698581643374 1105 18 1.066458632177067 1105 19 1.2218883922219916 1105 20 1.1353667968340915 1105 21 1.1206371211224067 1105 22 1.1114763108526162 1105 23 1.2100812147254985 1105 24 1.1308726146642343 1105 25 1.1750851868014252 1106 1 0.5458538603039358 1106 2 0.5572757243949837 1106 3 0.5269388066926055 1106 4 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-3.432015986559744 2216 24 -3.7574677573299518 2216 25 -3.491613444719242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51567.0 3 1.0 4 817.0 5 7098.0 6 8317.0 7 6400.0 8 5219.0 9 4650.0 10 4006.0 11 3772.0 12 3370.0 13 3297.0 14 3149.0 15 3048.0 16 3144.0 17 3382.0 18 3745.0 19 4357.0 20 5582.0 21 7352.0 22 9250.0 23 11089.0 24 13213.0 25 15330.0 26 17354.0 27 19193.0 28 21876.0 29 26121.0 30 33166.0 31 42268.0 32 53773.0 33 69355.0 34 90196.0 35 118105.0 36 162499.0 37 253809.0 38 843959.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.57750402313015 19.387966439742105 13.497886432783176 27.536643104344567 2 13.871391490288577 20.829748342712513 39.41811657225401 25.8807435947449 3 16.80148631875867 24.77270363803523 30.418831671089375 28.006978372116727 4 11.549489086151501 17.564112649465073 37.56753875327084 33.31885951111258 5 12.89808158692091 37.94411066031269 35.99841150509375 13.159396247672658 6 31.622865971737525 37.74172536560411 17.874680788930288 12.760727873728076 7 27.503108020400624 31.7832221761008 22.651387248472904 18.06228255502568 8 25.08629888954415 36.62562669277595 20.58379315397524 17.704281263704658 9 26.1318899421094 14.980067265537361 19.951939876184614 38.93610291616863 10 14.39960965381639 29.10228665374491 34.82011235920137 21.677991333237333 11 34.2171845216783 22.458188373252977 24.637194497277406 18.687432607791315 12 22.570230707771536 24.97795587863935 31.860844672774164 20.59096874081495 13 28.628500644712634 21.83759657753834 25.9316252760467 23.602277501702332 14 21.040964788050985 21.616817869710978 27.735948931264492 29.606268410973545 15 22.95932596816014 30.289313565237457 23.487886064899833 23.26347440170257 16 22.559593209041847 28.045589070132216 26.898876160284164 22.495941560541773 17 21.85262722061925 28.305976425530815 27.49766454901859 22.343731804831343 18 21.8138460877794 27.652491725636224 28.77414207640095 21.75952011018343 19 23.31994623082869 27.028166725823795 27.55525154886465 22.096635494482864 20 23.46509632827675 27.145512252013027 27.742874853440487 21.646516566269735 21 23.123272277435188 26.624996960398256 27.315077097234663 22.936653664931896 22 23.083223174489024 27.471589365769617 27.225885560697737 22.21930189904362 23 22.50907866138184 27.289325646500544 27.472373396715383 22.72922229540223 24 22.89194899908321 26.93483331160352 27.83993195462814 22.333285734685134 25 22.908283562899083 27.05909379952574 27.572104824994163 22.460517812581006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 173.0 1 173.0 2 315.0 3 457.0 4 457.0 5 457.0 6 1025.5 7 1594.0 8 1594.0 9 1594.0 10 2476.0 11 3358.0 12 3358.0 13 3358.0 14 5958.5 15 8559.0 16 8559.0 17 8559.0 18 15664.5 19 22770.0 20 22770.0 21 22770.0 22 35843.0 23 48916.0 24 48916.0 25 48916.0 26 68927.5 27 88939.0 28 88939.0 29 88939.0 30 113632.0 31 138325.0 32 138325.0 33 138325.0 34 158955.5 35 179586.0 36 179586.0 37 179586.0 38 196749.0 39 213912.0 40 213912.0 41 213912.0 42 226859.5 43 239807.0 44 239807.0 45 239807.0 46 245146.0 47 250485.0 48 250485.0 49 250485.0 50 243296.5 51 236108.0 52 236108.0 53 236108.0 54 213753.5 55 191399.0 56 191399.0 57 191399.0 58 165173.0 59 138947.0 60 138947.0 61 138947.0 62 113094.0 63 87241.0 64 87241.0 65 87241.0 66 67468.5 67 47696.0 68 47696.0 69 47696.0 70 34737.5 71 21779.0 72 21779.0 73 21779.0 74 15159.0 75 8539.0 76 8539.0 77 8539.0 78 5789.5 79 3040.0 80 3040.0 81 3040.0 82 1973.0 83 906.0 84 906.0 85 906.0 86 568.0 87 230.0 88 230.0 89 230.0 90 141.5 91 53.0 92 53.0 93 53.0 94 31.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07745123857309674 2 1.0347526863473179E-4 3 0.0 4 0.03130126876200636 5 0.07455393105132425 6 0.07786513964763567 7 0.03782021068599447 8 0.07553694610335421 9 0.055980120331389896 10 0.11563361269931276 11 0.005846352677862346 12 0.01764253330222177 13 0.00957146234871269 14 0.011278804281185766 15 0.011382279549820497 16 0.02255760856237153 17 0.0338364128435573 18 0.0026903569845030265 19 0.006363729021036005 20 0.008640184931000104 21 5.173763431736589E-4 22 7.243268804431225E-4 23 0.0 24 5.1737634317365895E-5 25 0.006311991386718638 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1932829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.3870983936385 #Duplication Level Percentage of deduplicated Percentage of total 1 83.26070242699414 61.93522063760268 2 11.66846079344612 17.359658822887795 3 2.8097646455739946 6.270307174598385 4 0.9770385353713865 2.907162466601911 5 0.44312591675248153 1.6481425585119052 6 0.2359553467404327 1.0531220156691392 7 0.1364271838427879 0.7103895643656329 8 0.10217303011825084 0.6080284195666011 9 0.06452834528638973 0.4320068732997616 >10 0.27717427580191517 3.4580707374438595 >50 0.012728845279353751 0.6576974054148127 >100 0.010387799312862168 1.5656715927305418 >500 9.057782381258974E-4 0.48056216058419293 >1k 6.27077241779466E-4 0.9139595707227829 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3515 0.1818577846255411 No Hit TATCAACGCAGAGTACTTTTTTTTT 2500 0.12934408579341472 No Hit GGTATCAACGCAGAGTACTTTTTTT 2225 0.11511623635613911 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.1737634317365895E-5 0.0 5 0.0 5.1737634317365895E-5 0.0 5.1737634317365895E-5 0.0 6 0.0 5.1737634317365895E-5 0.0 5.1737634317365895E-5 5.1737634317365895E-5 7 0.0 5.1737634317365895E-5 0.0 5.1737634317365895E-5 5.1737634317365895E-5 8 0.0 5.1737634317365895E-5 0.0 5.1737634317365895E-5 5.1737634317365895E-5 9 0.0 5.1737634317365895E-5 0.0 1.5521290295209767E-4 5.1737634317365895E-5 10 0.0 5.1737634317365895E-5 0.0 1.5521290295209767E-4 5.1737634317365895E-5 11 0.0 5.1737634317365895E-5 0.0 1.5521290295209767E-4 5.1737634317365895E-5 12 0.0 5.1737634317365895E-5 0.0 1.5521290295209767E-4 1.0347526863473179E-4 13 0.0 5.1737634317365895E-5 0.0 1.5521290295209767E-4 1.5521290295209767E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCTG 45 6.681403E-4 14.801564 5 TTCGCAG 40 0.0052733226 14.252603 10 GGTATCA 730 0.0 12.633121 1 GGCGAGG 455 0.0 12.096436 19 TAGGACA 455 0.0 11.711127 4 GTACCGT 75 2.0474958E-4 11.415981 6 GTATCAA 1520 0.0 10.195436 1 TAGGACC 430 0.0 10.179149 4 CGGATCA 75 0.0026412187 10.1364975 9 TGTAGGA 1150 0.0 10.085327 2 TTATACT 305 0.0 9.983256 4 CTTAGAC 200 2.5465852E-11 9.982511 3 AGGACGT 945 0.0 9.9684 5 CGAAATC 105 4.1454434E-5 9.943639 13 GTCCTAC 1025 0.0 9.924797 1 TATGGCG 1000 0.0 9.871321 16 TAAGGGT 135 6.303071E-7 9.867709 4 TGGCGAG 930 0.0 9.795557 18 GTAGGAC 1035 0.0 9.736794 3 GGACGTG 965 0.0 9.56261 6 >>END_MODULE