FastQCFastQC Report
Thu 26 May 2016
SRR1512929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21075
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA6523.0937129300118626No Hit
GTATCAACGCAGAGTACTTTTTTTT3961.8790035587188614No Hit
GTACATGGGAAGCAGTGGTATCAAC3591.7034400948991695No Hit
CCCATGTACTCTGCGTTGATACCAC3191.5136417556346382No Hit
GGTATCAACGCAGAGTACTTTTTTT2561.214709371293001No Hit
TATCAACGCAGAGTACTTTTTTTTT2501.1862396204033214No Hit
GAGTACATGGGAAGCAGTGGTATCA1490.7069988137603795No Hit
CATGTACTCTGCGTTGATACCACTG1170.5551601423487544No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1040.49347568208778175No Hit
GTATCAACGCAGAGTACATGGGAAG990.46975088967971534No Hit
GCGTTGATACCACTGCTTCCCATGT990.46975088967971534No Hit
GTACTTTTTTTTTTTTTTTTTTTTT970.4602609727164887No Hit
GGTATCAACGCAGAGTACATGGGAA930.4412811387900356No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA910.431791221826809No Hit
ACGCAGAGTACATGGGAAGCAGTGG890.42230130486358247No Hit
TATCAACGCAGAGTACATGGGAAGC790.3748517200474496No Hit
ACTCTGCGTTGATACCACTGCTTCC770.365361803084223No Hit
GAGTACTTTTTTTTTTTTTTTTTTT670.3179122182680902No Hit
GTGGTATCAACGCAGAGTACATGGG650.3084223013048636No Hit
GGGAAGCAGTGGTATCAACGCAGAG530.2514827995255041No Hit
GCAGAGTACTTTTTTTTTTTTTTTT520.24673784104389088No Hit
GCTTCCCATGTACTCTGCGTTGATA470.22301304863582444No Hit
GTACTCTGCGTTGATACCACTGCTT440.20877817319098457No Hit
GAAACAACACAAAAAAAAAAAAAAA440.20877817319098457No Hit
GATACCACTGCTTCCCATGTACTCT380.18030842230130487No Hit
GCAGTGGTATCAACGCAGAGTACAT370.17556346381969157No Hit
CAGTGGTATCAACGCAGAGTACATG370.17556346381969157No Hit
GTGGTATCAACGCAGAGTACTTTTT370.17556346381969157No Hit
ACATGGGAAGCAGTGGTATCAACGC360.1708185053380783No Hit
ATACCACTGCTTCCCATGTACTCTG360.1708185053380783No Hit
GCAGAGTACATGGGAAGCAGTGGTA350.166073546856465No Hit
GAACAAAAAAAAAAAAAAAAAAAAA310.14709371293001186No Hit
TAACAACACAAAAAAAAAAAAAAAA300.1423487544483986No Hit
CATGGGAAGCAGTGGTATCAACGCA300.1423487544483986No Hit
ATCAACGCAGAGTACTTTTTTTTTT300.1423487544483986No Hit
GTACTGGTTCACTATCGGTCAGTCA290.1376037959667853No Hit
ACACAAAAAAAAAAAAAAAAAAAAA270.12811387900355872No Hit
CTGCTTCCCATGTACTCTGCGTTGA270.12811387900355872No Hit
AAGCAGTGGTATCAACGCAGAGTAC270.12811387900355872No Hit
CCACTGCTTCCCATGTACTCTGCGT250.11862396204033215No Hit
TACCACTGCTTCCCATGTACTCTGC250.11862396204033215No Hit
GGAAACAACACAAAAAAAAAAAAAA240.11387900355871886No Hit
GTTGATACCACTGCTTCCCATGTAC240.11387900355871886No Hit
CTTCCCATGTACTCTGCGTTGATAC240.11387900355871886No Hit
CCATGTACTCTGCGTTGATACCACT230.10913404507710558No Hit
GAATGGAATGGAATGGAATGGAATG220.10438908659549229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC1052.9454532E-610.86394517
TGCGTTG1157.2990224E-710.74585912
TTGATAC1157.2990224E-710.74585916
GTTGATA1201.2632099E-610.29811615
TACTCTG1201.3030167E-610.2736557
CCATGTA1053.582396E-59.9586162
GATACCA1158.74545E-69.91925518
GCGTTGA1252.1336564E-69.8861913
CGTTGAT1252.1336564E-69.8861914
GTACTCT1252.200637E-69.8627086
ACTCTGC1252.200637E-69.8627088
CTGCGTT1252.200637E-69.86270811
CTCTGCG1303.6342371E-69.4833739
TCTGCGT1303.6342371E-69.48337310
ATACCAC1106.1329905E-59.48337319
CCCATGT1053.8269756E-49.0966051
CATGTAC1159.715736E-59.092653
GAAGCAG950.00170002418.9842499
GGAAGCA950.00170002418.9842498
GTGGTAT1106.394475E-48.64177515
Produced by FastQC (version 0.11.5)