##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512929_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21075
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.08526690391459	30.0	12.0	31.0	2.0	34.0
2	23.114021352313166	30.0	12.0	31.0	2.0	34.0
3	23.280664294187424	30.0	15.0	31.0	2.0	34.0
4	25.862680901542113	35.0	16.0	37.0	2.0	37.0
5	26.19250296559905	35.0	16.0	37.0	2.0	37.0
6	25.80749703440095	35.0	10.0	37.0	2.0	37.0
7	25.605077105575326	35.0	8.0	37.0	2.0	37.0
8	25.20194543297746	35.0	7.0	37.0	2.0	37.0
9	25.633689205219454	35.0	2.0	39.0	2.0	39.0
10	25.679905100830368	35.0	2.0	39.0	2.0	39.0
11	25.58846975088968	35.0	2.0	39.0	2.0	39.0
12	25.63397390272835	35.0	2.0	39.0	2.0	39.0
13	25.394163701067615	35.0	2.0	39.0	2.0	39.0
14	26.130106761565838	35.0	2.0	40.0	2.0	41.0
15	25.95905100830368	34.0	2.0	40.0	2.0	41.0
16	25.903629893238435	34.0	2.0	40.0	2.0	41.0
17	25.905907473309608	34.0	2.0	40.0	2.0	41.0
18	25.719952550415183	34.0	2.0	40.0	2.0	41.0
19	25.817461447212338	34.0	2.0	40.0	2.0	41.0
20	25.868801897983392	35.0	2.0	40.0	2.0	41.0
21	25.840569395017795	35.0	2.0	40.0	2.0	41.0
22	25.759335705812575	35.0	2.0	40.0	2.0	41.0
23	25.557769869513642	34.0	2.0	40.0	2.0	41.0
24	25.562419928825623	35.0	2.0	40.0	2.0	41.0
25	25.457841043890866	35.0	2.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5052221867531195
1101	2	0.8618903264017668
1101	3	0.9694626311356807
1101	4	0.4106245182758528
1101	5	0.6154495641090669
1101	6	-0.0674090437893824
1101	7	1.077611979492147
1101	8	0.7240704297111726
1101	9	1.543087821949257
1101	10	1.6428088510978718
1101	11	1.645495237463372
1101	12	0.30736985259144944
1101	13	2.0338790797091946
1101	14	0.49968971566215714
1101	15	-1.4657919055641564
1101	16	0.36017306797524284
1101	17	1.3973924437039074
1101	18	0.36465236435552484
1101	19	0.37347730516027866
1101	20	1.0488037681600701
1101	21	0.7528605443944549
1101	22	0.7136618190480455
1101	23	1.8956882205082835
1101	24	0.1388380280833914
1101	25	-0.4288934280814338
1102	1	3.80522218675312
1102	2	3.98569985021129
1102	3	3.531367393040444
1102	4	5.272529280180613
1102	5	4.834497183156689
1102	6	4.070686194305857
1102	7	3.787135789015956
1102	8	3.9859751916159354
1102	9	4.038325917187354
1102	10	4.033285041574061
1102	11	4.164542856510991
1102	12	3.612131757353353
1102	13	3.771974317804432
1102	14	4.713975429947872
1102	15	4.877065237292989
1102	16	5.641125448927625
1102	17	4.459297205608667
1102	18	4.850366650069809
1102	19	5.363953495636469
1102	20	4.42975614911245
1102	21	4.000479592013505
1102	22	5.1755665809528075
1102	23	5.200450125270191
1102	24	4.810266599511962
1102	25	5.566344667156663
1103	1	1.5923189609466668
1103	2	2.8704924769394005
1103	3	1.9027959644690142
1103	4	1.3407320451575728
1103	5	0.27781515550691793
1103	6	3.563773751909544
1103	7	2.3862141300297814
1103	8	4.344500537238055
1103	9	3.861367391841732
1103	10	2.9954970231408815
1103	11	3.67237695789348
1103	12	3.3439289923763944
1103	13	3.148932843150053
1103	14	3.70291552211377
1103	15	4.585820997661653
1103	16	3.4171623152870687
1103	17	2.1016935189727235
1103	18	2.629168493387784
1103	19	3.661649348171032
1103	20	1.605793015471896
1103	21	1.705548716437466
1103	22	2.411511281413638
1103	23	2.2096667151319416
1103	24	2.0581928667930676
1103	25	2.092611948262654
1104	1	-0.24639071647268906
1104	2	0.3866215091974645
1104	3	-0.09720403553098578
1104	4	-1.5624937612940393
1104	5	-0.7221848444930821
1104	6	-0.5975165706711039
1104	7	-0.29120522480892674
1104	8	-3.010338172439365
1104	9	-2.719277769448592
1104	10	-3.3270836220204068
1104	11	-3.7147198163000716
1104	12	-3.591554878591346
1104	13	-3.076873608462847
1104	14	-2.3616006069184863
1104	15	-3.027082228144799
1104	16	-2.8086441363258317
1104	17	-2.5434677713498566
1104	18	-2.30631537757996
1104	19	-2.854479684087032
1104	20	-2.1038844038829403
1104	21	-2.06864483194963
1104	22	-3.2336500089089455
1104	23	-3.338720381642254
1104	24	-3.038581326755317
1104	25	-2.552549342059926
1105	1	1.2337936153245472
1105	2	1.214271278782718
1105	3	0.645653107326158
1105	4	0.7582435658949009
1105	5	0.03449718315668804
1105	6	2.2992576228772847
1105	7	1.0728500747302405
1105	8	-1.0711676655269216
1105	9	-0.3045312256697912
1105	10	0.17614218443120677
1105	11	0.2502571422252764
1105	12	1.1549889002104976
1105	13	0.4291171749472902
1105	14	0.3711182870907308
1105	15	1.2770652372929874
1105	16	1.3268397346419079
1105	17	1.7450114913229555
1105	18	1.3646523643555248
1105	19	1.4496677813507546
1105	20	0.7154704348267344
1105	21	0.029051020584933696
1105	22	0.08985229523852212
1105	23	1.6575929824130462
1105	24	1.1531237423691074
1105	25	1.5377732385852347
1106	1	-1.1947778132468798
1106	2	-0.43810967359823394
1106	3	0.9361292978023492
1106	4	1.3439578516091863
1106	5	-0.18455043589093023
1106	6	-1.467409043789381
1106	7	-1.4890546871745194
1106	8	-0.07592957028882807
1106	9	-0.9902455113840745
1106	10	1.0761421844312054
1106	11	-0.6545047625366287
1106	12	1.6407031859247816
1106	13	0.100545746375861
1106	14	0.4663563823288257
1106	15	-0.19912523889748712
1106	16	-0.9731602653580929
1106	17	-1.702607556296094
1106	18	-0.33534763564447445
1106	19	-2.059856028173055
1106	20	-0.817862898506597
1106	21	-0.8138061222722115
1106	22	-0.11967151428528666
1106	23	0.12902155384161773
1106	24	-0.22782863858327573
1106	25	0.9044399052519019
1107	1	-0.1461291645982321
1107	2	-1.2498213853099465
1107	3	0.2541473158203651
1107	4	-0.08036647271513786
1107	5	-0.06743331877381564
1107	6	-1.2034450798254177
1107	7	-0.6259916241114567
1107	8	-1.0696232639825212
1107	9	-2.347002268140834
1107	10	-1.5319659236769034
1107	11	-2.440090348122215
1107	12	-3.478215732994137
1107	13	-2.9129677671376513
1107	14	-2.1462562302837895
1107	15	-3.271197310969562
1107	16	-2.7380251302229546
1107	17	-2.4719769256654622
1107	18	-2.5434557437525847
1107	19	-2.0715677398847667
1107	20	-2.143989024632724
1107	21	-2.119211527677617
1107	22	-1.6530048476186217
1107	23	-1.1277352029151402
1107	24	-1.7935944043490402
1107	25	-1.9649294641174677
1108	1	-1.961444479913549
1108	2	-1.593665229153789
1108	3	-2.0416484799754286
1108	4	-2.617153259501926
1108	5	-1.6845504358909302
1108	6	-2.056297932678273
1108	7	-2.0501657982856294
1108	8	-1.6537073480666038
1108	9	-1.3124677336062973
1108	10	-0.06274670445768393
1108	11	-3.2156158736477387
1108	12	-1.2815190362974391
1108	13	-1.8161209202908069
1108	14	-1.4780880621156207
1108	15	-1.5324585722308228
1108	16	-1.3453824875803129
1108	17	-0.8803853340738712
1108	18	-1.0742365245333652
1108	19	-0.7209671392841663
1108	20	-2.128974009617707
1108	21	-2.0471394556055422
1108	22	-1.6807826253964002
1108	23	-1.3154228906028251
1108	24	-1.7500508608054979
1108	25	-1.3733378725258767
1109	1	-1.6697778132468812
1109	2	-0.8964430069315661
1109	3	-0.42220403553098507
1109	4	-0.5310421483908137
1109	5	-0.30121710255759737
1109	6	-0.7007423771227153
1109	7	-1.255721353841185
1109	8	0.20740376304450692
1109	9	0.7597544886159255
1109	10	0.20114218443120535
1109	11	0.37882857079670273
1109	12	0.7657031859247816
1109	13	0.6505457463758617
1109	14	-0.025310284337841438
1109	15	0.9842080944358464
1109	16	0.051839734641909274
1109	17	0.9307257770372388
1109	18	0.4646523643555227
1109	19	1.356810638493613
1109	20	1.5904704348267344
1109	21	0.3611938777277892
1109	22	0.8969951523813791
1109	23	0.004021553841617731
1109	24	0.8888380280833914
1109	25	1.362773238585234
1110	1	2.8614721867531188
1110	2	2.5660569930684325
1110	3	2.8402959644690142
1110	4	2.650207851609185
1110	5	2.742532897442402
1110	6	3.218007622877284
1110	7	3.0505286461588135
1110	8	3.769903763044507
1110	9	3.816004488615924
1110	10	3.7011421844312054
1110	11	4.728828570796704
1110	12	2.0344531859247823
1110	13	3.788045746375861
1110	14	3.362189715662158
1110	15	2.977958094435845
1110	16	3.1893397346419086
1110	17	3.7932257770372395
1110	18	2.689652364355524
1110	19	2.9630606384936122
1110	20	1.8779704348267359
1110	21	2.50494387772779
1110	22	3.3157451523813783
1110	23	2.5665215538416177
1110	24	3.5138380280833914
1110	25	3.550273238585234
1111	1	-6.472197168085589
1111	2	-5.323055910157375
1111	3	-5.645591132305178
1111	4	-7.497977632261783
1111	5	-6.883475167073726
1111	6	-5.274935925509812
1111	7	-4.7105600635186065
1111	8	-7.171628495020009
1111	9	-7.10637454364214
1111	10	-7.327083622020407
1111	11	-6.295364977590392
1111	12	-7.430264556010702
1111	13	-5.722034898785431
1111	14	-7.813213510144294
1111	15	-6.156114486209315
1111	16	-6.937676394390351
1111	17	-6.672500029414373
1111	18	-7.080508925967056
1111	19	-6.628673232474128
1111	20	-5.87807795227004
1111	21	-5.617031928723822
1111	22	-6.523972589554106
1111	23	-6.532268768739026
1111	24	-6.1998716493359645
1111	25	-7.036420309801862
1112	1	-5.71583044482583
1112	2	-3.5556535332473587
1112	3	-2.8340461407941433
1112	4	-3.0455158326013425
1112	5	-4.035427628873389
1112	6	-3.7639002718595584
1112	7	-5.19782661699908
1112	8	-4.542596236955493
1112	9	-5.721824458752497
1112	10	-4.976489394516165
1112	11	-5.389592481834875
1112	12	-3.848770498285745
1112	13	-4.873138464150454
1112	14	-5.068731336969421
1112	15	-4.997370852932576
1112	16	-4.767897107463355
1112	17	-4.348221591383812
1112	18	-5.040610793539212
1112	19	-5.5616104141379665
1112	20	-6.105582196752213
1112	21	-5.582227174903789
1112	22	-6.258268005513358
1112	23	-5.134136340895225
1112	24	-5.334846182442924
1112	25	-5.446437287730557
1113	1	2.1169868926354702
1113	2	2.5991452283625485
1113	3	1.5792665527043077
1113	4	1.5674872633738914
1113	5	0.9134887797953439
1113	6	0.9698458581714
1113	7	1.820749234394107
1113	8	2.8985802336327424
1113	9	3.7332839003806306
1113	10	3.2232010079606184
1113	11	2.331769747267291
1113	12	2.2936443623953693
1113	13	2.4240751581405675
1113	14	2.652630892132745
1113	15	2.4283257414946675
1113	16	2.332722087583086
1113	17	2.5836669535078265
1113	18	2.2411229525908176
1113	19	2.3068106384936122
1113	20	3.433117493650265
1113	21	3.6097232894924964
1113	22	1.2881716229696138
1113	23	2.8643156714886757
1113	24	2.697661557495156
1113	25	4.355420297408763
1114	1	-3.8260278132468812
1114	2	-2.6214430069315675
1114	3	-2.0659540355309858
1114	4	-2.412292148390815
1114	5	-4.038717102557598
1114	6	-2.625742377122716
1114	7	-4.4182213538411865
1114	8	-1.386346236955493
1114	9	-2.808995511384076
1114	10	-1.8301078155687946
1114	11	-3.396171429203296
1114	12	-2.9655468140752177
1114	13	-3.993204253624139
1114	14	-5.762810284337842
1114	15	-4.490791905564155
1114	16	-5.466910265358091
1114	17	-10.26927422296276
1114	18	-7.685347635644476
1114	19	-5.505689361506388
1114	20	-4.184529565173264
1114	21	-4.96380612227221
1114	22	-5.371754847618622
1114	23	-5.495978446158382
1114	24	-3.6424119719166086
1114	25	-3.074726761414766
1115	1	-4.394777813246883
1115	2	-4.185728721217281
1115	3	-3.0114897498167004
1115	4	-4.127470719819385
1115	5	-3.0512171025575974
1115	6	-4.757885234265572
1115	7	-5.270007068126901
1115	8	-4.271167665526921
1115	9	-5.418816939955505
1115	10	-5.909572101283079
1115	11	-6.578314286346153
1115	12	-5.216439671218076
1115	13	-5.456597110766996
1115	14	-4.800310284337844
1115	15	-5.494363334135585
1115	16	-4.3017316939295185
1115	17	-4.426417080105619
1115	18	-3.5210619213587613
1115	19	-4.150332218649243
1115	20	-3.684529565173264
1115	21	-4.628091836557925
1115	22	-4.224433419047195
1115	23	-3.170978446158383
1115	24	-3.5897334004880364
1115	25	-4.262226761414766
1116	1	-3.8830131073645298
1116	2	-3.959678301049216
1116	3	-2.8030863884721633
1116	4	-2.403100971920228
1116	5	-2.7629818084399496
1116	6	-1.5889776712403645
1116	7	-0.5027801773705995
1116	8	-1.7190668251907866
1116	9	-1.7078925702076049
1116	10	-3.3944460508629106
1116	11	-0.6388184880268248
1116	12	0.14658553886595982
1116	13	-0.8112189595064905
1116	14	-0.49442793139666463
1116	15	0.487149270906432
1116	16	-0.43198379476985593
1116	17	-0.092803634727467
1116	18	-0.023582929762124394
1116	19	-1.9284834791534458
1116	20	-1.0080589769379706
1116	21	-1.5373355340369166
1116	22	-2.0353577887950927
1116	23	-1.9298019755701468
1116	24	-3.655279618975431
1116	25	-2.9681091143559435
1201	1	2.136256669511738
1201	2	1.5078673378960197
1201	3	0.385554585158669
1201	4	1.5887854378160817
1201	5	1.1832656560630923
1201	6	1.4647748642565936
1201	7	2.95462347374502
1201	8	1.9229210044238165
1201	9	3.616651040340063
1201	10	2.989935287879483
1201	11	3.0650354673484266
1201	12	3.509668703166163
1201	13	3.083304367065516
1201	14	4.592793163938019
1201	15	-0.17613673315036138
1201	16	1.4027018036074246
1201	17	2.9893464666924103
1201	18	3.564652364355524
1201	19	2.1688796040108542
1201	20	0.31547043482673587
1201	21	0.42412491221054793
1201	22	1.8642365316917235
1201	23	1.008331898669205
1201	24	1.8112518211868398
1201	25	2.0481180661714404
1202	1	1.3052221867531202
1202	2	0.8952236597350982
1202	3	0.03612929780234708
1202	4	0.6106245182758521
1202	5	-0.3178837692242631
1202	6	0.29925762287728475
1202	7	0.4442786461588142
1202	8	0.7907370963778391
1202	9	0.24308782194925627
1202	10	0.17614218443120677
1202	11	-1.9211714292032944
1202	12	-1.792630147408552
1202	13	-0.7661209202908061
1202	14	-1.0669769510045093
1202	15	-0.19912523889748712
1202	16	-0.33982693202475645
1202	17	-1.335940889629427
1202	18	1.0646523643555241
1202	19	0.9068106384936137
1202	20	0.4821371014934037
1202	21	0.4528605443944578
1202	22	1.113661819048044
1202	23	0.09568822050828274
1202	24	0.23883802808339283
1202	25	0.8711065719185669
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2211	18	-2.7383779386747804
2211	19	-2.6628863312033566
2211	20	-2.4118022924459908
2211	21	-2.259260667726757
2211	22	-2.5317927264065005
2211	23	-2.8558269310068667
2211	24	-3.088434699189335
2211	25	-2.8076813068693127
2212	1	0.6337936153245458
2212	2	0.47141413592557413
2212	3	-0.6114897498167018
2212	4	0.9582435658949002
2212	5	0.32021146887097274
2212	6	-0.3007423771227167
2212	7	-1.384292782412615
2212	8	-0.8711676655269223
2212	9	-0.5331026542412189
2212	10	-1.1667149584259384
2212	11	-1.6640285720604382
2212	12	-1.5878682426466462
2212	13	-1.1137399679098543
2212	14	-1.6288817129092692
2212	15	-0.9515061912784404
2212	16	-1.1874459796438046
2212	17	-2.054988508677045
2212	18	-5.406776207073047
2212	19	-2.950332218649244
2212	20	-1.5702438508875503
2212	21	-2.1709489794150656
2212	22	-1.9672905619043348
2212	23	-1.4566927318726677
2212	24	-1.7611619719166072
2212	25	-0.4050839042719083
2213	1	-1.8830131073645298
2213	2	-2.783207712813919
2213	3	-2.744262859060399
2213	4	-3.138395089567286
2213	5	-3.6747465143223046
2213	6	-3.5595659065344805
2213	7	-4.1204272361941285
2213	8	-4.39553741342608
2213	9	-4.296127864325253
2213	10	-5.306210756745266
2213	11	-4.815289076262118
2213	12	-4.029885049369334
2213	13	-4.428866018330023
2213	14	-3.817957343161371
2213	15	-5.0422624937994485
2213	16	-4.314336735946327
2213	17	-4.210450693550996
2213	18	-3.170641753291534
2213	19	-3.9873070085652103
2213	20	-4.508058976937971
2213	21	-5.007923769331033
2213	22	-4.329475435853915
2213	23	-3.3709784461583823
2213	24	-2.919985501328373
2213	25	-3.0563444084735885
2214	1	-3.0006601661880588
2214	2	-3.3714430069315675
2214	3	-3.8913216825898083
2214	4	-2.9619245013319926
2214	5	-1.9100406319693626
2214	6	-2.7948600241815384
2214	7	-3.8263095891353025
2214	8	-3.572008001661377
2214	9	-3.8843631584428984
2214	10	-3.747387227333501
2214	11	-3.0799949586150603
2214	12	-2.000473284663453
2214	13	-3.9582777830359035
2214	14	-4.553251460808429
2214	15	-4.1599095526229775
2214	16	-4.696689677122798
2214	17	-3.1516271641392315
2214	18	-1.8765241062327114
2214	19	-2.6637775968005037
2214	20	-2.3610001534085576
2214	21	-1.860864945801623
2214	22	-2.2706519064421506
2214	23	-2.5474490343936758
2214	24	-1.802338442504844
2214	25	-2.144579702591237
2215	1	-4.251920670389737
2215	2	-6.471443006931569
2215	3	-4.440061178388127
2215	4	-3.3560421483908165
2215	5	-3.8797885311290266
2215	6	-4.986456662837
2215	7	-4.955721353841188
2215	8	-4.0711676655269216
2215	9	-3.1331026542412204
2215	10	-6.195286386997367
2215	11	-3.2640285720604396
2215	12	-4.387868242646647
2215	13	-4.170882825052711
2215	14	-5.371738855766413
2215	15	-5.522934762707013
2215	16	-4.330303122500947
2215	17	-4.512131365819904
2215	18	-4.8353476356444745
2215	19	-5.207475075792104
2215	20	-4.627386708030407
2215	21	-4.570948979415068
2215	22	-4.624433419047193
2215	23	-4.1424070175869545
2215	24	-4.104019114773752
2215	25	-4.690798189986193
2216	1	-6.208570916695159
2216	2	-6.043856800035016
2216	3	-6.028238518289605
2216	4	-8.031904217356331
2216	5	-7.333975723247253
2216	6	-6.293845825398577
2216	7	-7.666066181427393
2216	8	-7.146044512817561
2216	9	-8.762659304487524
2216	10	-6.97558195349983
2216	11	-7.555654187823986
2216	12	-8.17998646924763
2216	13	-7.020143908796552
2216	14	-7.786517180889565
2216	15	-8.3830332848745
2216	16	-8.666263713633953
2216	17	-7.73479146434207
2216	18	-7.5387959115065435
2216	19	-7.58974108564432
2216	20	-7.546598530690506
2216	21	-8.334495777444623
2216	22	-8.480591054515173
2216	23	-7.853737066848037
2216	24	-8.292196454675228
2216	25	-9.331192278656145
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	4360.0
3	0.0
4	27.0
5	165.0
6	331.0
7	234.0
8	199.0
9	139.0
10	129.0
11	118.0
12	139.0
13	108.0
14	80.0
15	80.0
16	62.0
17	73.0
18	84.0
19	93.0
20	164.0
21	212.0
22	292.0
23	309.0
24	276.0
25	272.0
26	293.0
27	251.0
28	322.0
29	322.0
30	335.0
31	426.0
32	482.0
33	631.0
34	791.0
35	1038.0
36	1561.0
37	2357.0
38	4320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.44535974477406	21.2942240845674	11.347078710537593	19.913337460120946
2	15.5646271419756	26.52489675796269	35.79531969430864	22.115156405753073
3	19.660148091892918	34.51680273400417	25.716726789443705	20.106322384659197
4	12.244897959183673	22.325581395348838	31.238728049359278	34.19079259610821
5	11.833111828365293	43.49724700968293	29.974368710841087	14.69527245111069
6	28.97684130599848	38.041002277904326	16.45785876993166	16.52429764616553
7	24.278663629460894	38.876233864844345	19.480827638572514	17.364274867122248
8	26.331276696725205	36.55434266729948	18.993830090175607	18.120550545799716
9	26.9672520170859	19.833887043189367	17.81205505457997	35.38680588514475
10	17.987660180351213	34.10536307546274	26.336022781205504	21.57095396298054
11	29.20127189027573	31.51725119832946	17.621375350007117	21.66010156138769
12	23.27479829140959	35.775984812529664	24.6843853820598	16.26483151400095
13	28.927384907451355	29.65828191741813	21.07736117702895	20.336971998101568
14	21.025154247745608	31.73232083531087	24.105363075462744	23.137161841480776
15	27.599791162371258	32.099292799848115	20.931225971806917	19.369690065973703
16	19.39250118652112	35.007119126720454	25.334598955861416	20.26578073089701
17	19.966777408637874	35.41053630754627	24.855244423350737	19.767441860465116
18	19.631318890155836	32.04104903078677	28.00266058532877	20.32497149372862
19	20.142382534409112	33.69719981015662	29.39250118652112	16.767916468913146
20	22.600854295206453	30.71191267204556	27.707641196013288	18.979591836734695
21	21.661129568106315	32.406264831514	27.70289511153299	18.229710488846703
22	20.488846701471285	32.2828666350261	26.540104413858568	20.688182249644044
23	19.05078310393925	33.33649738965354	28.381585192216424	19.231134314190793
24	20.246796392975792	33.97247271001424	27.16658756525866	18.614143331751304
25	20.20408163265306	31.1390602752729	26.264831514000946	22.39202657807309
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	677.0
1	677.0
2	442.0
3	207.0
4	207.0
5	207.0
6	251.5
7	296.0
8	296.0
9	296.0
10	289.5
11	283.0
12	283.0
13	283.0
14	265.5
15	248.0
16	248.0
17	248.0
18	284.0
19	320.0
20	320.0
21	320.0
22	420.5
23	521.0
24	521.0
25	521.0
26	760.5
27	1000.0
28	1000.0
29	1000.0
30	1168.0
31	1336.0
32	1336.0
33	1336.0
34	1441.5
35	1547.0
36	1547.0
37	1547.0
38	1639.0
39	1731.0
40	1731.0
41	1731.0
42	1834.5
43	1938.0
44	1938.0
45	1938.0
46	2714.0
47	3490.0
48	3490.0
49	3490.0
50	3197.5
51	2905.0
52	2905.0
53	2905.0
54	2331.0
55	1757.0
56	1757.0
57	1757.0
58	1442.5
59	1128.0
60	1128.0
61	1128.0
62	949.5
63	771.0
64	771.0
65	771.0
66	593.5
67	416.0
68	416.0
69	416.0
70	343.0
71	270.0
72	270.0
73	270.0
74	198.0
75	126.0
76	126.0
77	126.0
78	97.0
79	68.0
80	68.0
81	68.0
82	46.5
83	25.0
84	25.0
85	25.0
86	17.0
87	9.0
88	9.0
89	9.0
90	7.0
91	5.0
92	5.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35112692763938314
2	0.03795966785290628
3	0.033214709371293005
4	0.02372479240806643
5	0.033214709371293005
6	0.014234875444839857
7	0.014234875444839857
8	0.02372479240806643
9	0.02372479240806643
10	0.02372479240806643
11	0.01897983392645314
12	0.02372479240806643
13	0.02372479240806643
14	0.02372479240806643
15	0.028469750889679714
16	0.02372479240806643
17	0.02372479240806643
18	0.12811387900355872
19	0.02372479240806643
20	0.02372479240806643
21	0.02372479240806643
22	0.02372479240806643
23	0.02372479240806643
24	0.02372479240806643
25	0.02372479240806643
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	21075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.75214569813691	62.39145907473309
2	5.017095806294048	6.8232502965599044
3	1.2141511408833996	2.4768683274021353
4	0.6628986114018561	1.8030842230130486
5	0.2930709650408206	0.99644128113879
6	0.19538064336054708	0.797153024911032
7	0.11862396204033215	0.564650059311981
8	0.090712441560254	0.49347568208778175
9	0.06280092108017585	0.3843416370106762
>10	0.4465843276812504	6.362989323843417
>50	0.08373456144023446	4.559905100830368
>100	0.0558230409601563	9.252669039145907
>500	0.006977880120019537	3.0937129300118626
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	652	3.0937129300118626	No Hit
GTATCAACGCAGAGTACTTTTTTTT	396	1.8790035587188614	No Hit
GTACATGGGAAGCAGTGGTATCAAC	359	1.7034400948991695	No Hit
CCCATGTACTCTGCGTTGATACCAC	319	1.5136417556346382	No Hit
GGTATCAACGCAGAGTACTTTTTTT	256	1.214709371293001	No Hit
TATCAACGCAGAGTACTTTTTTTTT	250	1.1862396204033214	No Hit
GAGTACATGGGAAGCAGTGGTATCA	149	0.7069988137603795	No Hit
CATGTACTCTGCGTTGATACCACTG	117	0.5551601423487544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	104	0.49347568208778175	No Hit
GTATCAACGCAGAGTACATGGGAAG	99	0.46975088967971534	No Hit
GCGTTGATACCACTGCTTCCCATGT	99	0.46975088967971534	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	97	0.4602609727164887	No Hit
GGTATCAACGCAGAGTACATGGGAA	93	0.4412811387900356	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	91	0.431791221826809	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	89	0.42230130486358247	No Hit
TATCAACGCAGAGTACATGGGAAGC	79	0.3748517200474496	No Hit
ACTCTGCGTTGATACCACTGCTTCC	77	0.365361803084223	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	67	0.3179122182680902	No Hit
GTGGTATCAACGCAGAGTACATGGG	65	0.3084223013048636	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	53	0.2514827995255041	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	52	0.24673784104389088	No Hit
GCTTCCCATGTACTCTGCGTTGATA	47	0.22301304863582444	No Hit
GTACTCTGCGTTGATACCACTGCTT	44	0.20877817319098457	No Hit
GAAACAACACAAAAAAAAAAAAAAA	44	0.20877817319098457	No Hit
GATACCACTGCTTCCCATGTACTCT	38	0.18030842230130487	No Hit
GCAGTGGTATCAACGCAGAGTACAT	37	0.17556346381969157	No Hit
CAGTGGTATCAACGCAGAGTACATG	37	0.17556346381969157	No Hit
GTGGTATCAACGCAGAGTACTTTTT	37	0.17556346381969157	No Hit
ACATGGGAAGCAGTGGTATCAACGC	36	0.1708185053380783	No Hit
ATACCACTGCTTCCCATGTACTCTG	36	0.1708185053380783	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	35	0.166073546856465	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	31	0.14709371293001186	No Hit
TAACAACACAAAAAAAAAAAAAAAA	30	0.1423487544483986	No Hit
CATGGGAAGCAGTGGTATCAACGCA	30	0.1423487544483986	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	30	0.1423487544483986	No Hit
GTACTGGTTCACTATCGGTCAGTCA	29	0.1376037959667853	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	27	0.12811387900355872	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	27	0.12811387900355872	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	27	0.12811387900355872	No Hit
CCACTGCTTCCCATGTACTCTGCGT	25	0.11862396204033215	No Hit
TACCACTGCTTCCCATGTACTCTGC	25	0.11862396204033215	No Hit
GGAAACAACACAAAAAAAAAAAAAA	24	0.11387900355871886	No Hit
GTTGATACCACTGCTTCCCATGTAC	24	0.11387900355871886	No Hit
CTTCCCATGTACTCTGCGTTGATAC	24	0.11387900355871886	No Hit
CCATGTACTCTGCGTTGATACCACT	23	0.10913404507710558	No Hit
GAATGGAATGGAATGGAATGGAATG	22	0.10438908659549229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACC	105	2.9454532E-6	10.863945	17
TGCGTTG	115	7.2990224E-7	10.745859	12
TTGATAC	115	7.2990224E-7	10.745859	16
GTTGATA	120	1.2632099E-6	10.298116	15
TACTCTG	120	1.3030167E-6	10.273655	7
CCATGTA	105	3.582396E-5	9.958616	2
GATACCA	115	8.74545E-6	9.919255	18
GCGTTGA	125	2.1336564E-6	9.88619	13
CGTTGAT	125	2.1336564E-6	9.88619	14
GTACTCT	125	2.200637E-6	9.862708	6
ACTCTGC	125	2.200637E-6	9.862708	8
CTGCGTT	125	2.200637E-6	9.862708	11
CTCTGCG	130	3.6342371E-6	9.483373	9
TCTGCGT	130	3.6342371E-6	9.483373	10
ATACCAC	110	6.1329905E-5	9.483373	19
CCCATGT	105	3.8269756E-4	9.096605	1
CATGTAC	115	9.715736E-5	9.09265	3
GAAGCAG	95	0.0017000241	8.984249	9
GGAAGCA	95	0.0017000241	8.984249	8
GTGGTAT	110	6.394475E-4	8.641775	15
>>END_MODULE