FastQCFastQC Report
Thu 26 May 2016
SRR1512929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21075
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7063.3499406880189797No Hit
TATCAACGCAGAGTACTTTTTTTTT6032.8612099644128115No Hit
GGTATCAACGCAGAGTACTTTTTTT4992.3677342823250296No Hit
GTACATGGGAAGCAGTGGTATCAAC3521.6702253855278766No Hit
CCCATGTACTCTGCGTTGATACCAC3221.527876631079478No Hit
CTTATACACATCTCCGAGCCCACGA2771.3143534994068802No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2691.276393831553974No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2501.1862396204033214No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1920.9110320284697508No Hit
ATACACATCTCCGAGCCCACGAGAC1570.7449584816132859No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1510.7164887307236062No Hit
GAGTACATGGGAAGCAGTGGTATCA1370.6500593119810202No Hit
TATCAACGCAGAGTACATGGGAAGC1160.5504151838671412No Hit
ACGCAGAGTACATGGGAAGCAGTGG1160.5504151838671412No Hit
ACACATCTCCGAGCCCACGAGACAA1100.5219454329774614No Hit
CATGTACTCTGCGTTGATACCACTG1080.5124555160142349No Hit
ACTCTGCGTTGATACCACTGCTTCC1050.498220640569395No Hit
GCAGAGTACTTTTTTTTTTTTTTTT890.42230130486358247No Hit
GCGTTGATACCACTGCTTCCCATGT870.4128113879003558No Hit
GGTATCAACGCAGAGTACATGGGAA820.38908659549228947No Hit
GTGGTATCAACGCAGAGTACTTTTT810.3843416370106762No Hit
ATCAACGCAGAGTACTTTTTTTTTT790.3748517200474496No Hit
GTATCAACGCAGAGTACATGGGAAG770.365361803084223No Hit
GTGGTATCAACGCAGAGTACATGGG650.3084223013048636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA620.2941874258600237No Hit
GTACTCTGCGTTGATACCACTGCTT480.2277580071174377No Hit
GGGAAGCAGTGGTATCAACGCAGAG460.21826809015421117No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT440.20877817319098457No Hit
GCTTCCCATGTACTCTGCGTTGATA440.20877817319098457No Hit
ACATGGGAAGCAGTGGTATCAACGC390.18505338078291814No Hit
TACCACTGCTTCCCATGTACTCTGC380.18030842230130487No Hit
ATACCACTGCTTCCCATGTACTCTG380.18030842230130487No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT360.1708185053380783No Hit
GCAGTGGTATCAACGCAGAGTACAT330.15658362989323843No Hit
TCAACGCAGAGTACTTTTTTTTTTT330.15658362989323843No Hit
CACATCTCCGAGCCCACGAGACAAG330.15658362989323843No Hit
CAACGCAGAGTACTTTTTTTTTTTT330.15658362989323843No Hit
GATACCACTGCTTCCCATGTACTCT320.15183867141162513No Hit
CATGGGAAGCAGTGGTATCAACGCA310.14709371293001186No Hit
CAGTGGTATCAACGCAGAGTACATG310.14709371293001186No Hit
AACGCAGAGTACTTTTTTTTTTTTT300.1423487544483986No Hit
AAGCAGTGGTATCAACGCAGAGTAC300.1423487544483986No Hit
GCAGAGTACATGGGAAGCAGTGGTA300.1423487544483986No Hit
TTCCCATGTACTCTGCGTTGATACC290.1376037959667853No Hit
CCACTGCTTCCCATGTACTCTGCGT280.13285883748517202No Hit
GTACATGGTAAGCAGTGGTATCAAC280.13285883748517202No Hit
CTGCTTCCCATGTACTCTGCGTTGA270.12811387900355872No Hit
GTACTGGTTCACTATCGGTCAGTCA260.12336892052194544No Hit
CTTCCCATGTACTCTGCGTTGATAC260.12336892052194544No Hit
ATTCCATTCCATTCCATTCCATTCC240.11387900355871886No Hit
ATCAACGCAGAGTACATGGGAAGCA240.11387900355871886No Hit
GTTGATACCACTGCTTCCCATGTAC240.11387900355871886No Hit
CCATGTACTCTGCGTTGATACCACT230.10913404507710558No Hit
ATGGGAAGCAGTGGTATCAACGCAG220.10438908659549229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2051.7510936E-56.97572661
GTACATG2301.1852766E-56.63199571
GTATCAA2804.8180154E-66.1286741
TACATGG2351.21202305E-46.07068542
ACATGGG2450.00138975955.43470863
AACGCAG3401.7782371E-55.3403165
ATCAACG3452.4637991E-55.23779872
CAACGCA3553.657629E-55.1146694
TATCAAC3553.8091297E-55.10243271
Produced by FastQC (version 0.11.5)