FastQCFastQC Report
Thu 26 May 2016
SRR1512928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297247
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35010.15239980724754457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1001.4398756E-712.35243518
TAGGACC5500.012.2609794
GGTATCA9650.012.149581
CGTGCGC907.510069E-611.60480510
GTCCTAC13250.010.7909281
CGGAACT803.7797095E-410.68518454
GTAGGAC13400.010.6320253
GGCGAGG5900.010.45996319
CTTATAC1951.4551915E-1110.2285533
AGGACGT12900.010.1605895
GGACGTG12700.010.0922816
GGACCGT856.6325E-410.0527046
GTTCTAA2950.010.0166651
GTATCAA20750.09.9683911
TGGACTG3750.09.8778595
TCCTACA14850.09.8497182
GTAGAAC4050.09.8497173
TGTAGGA15150.09.84275152
CTTAGAC1951.6370905E-109.7414793
AGGACCT10850.09.7167895
Produced by FastQC (version 0.11.5)