##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512928_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297247 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.096993488292725 31.0 30.0 34.0 23.0 34.0 2 29.23151406879626 31.0 30.0 34.0 23.0 34.0 3 29.455759437274267 31.0 30.0 34.0 23.0 34.0 4 32.903647278677475 35.0 35.0 37.0 26.0 37.0 5 33.05275401382612 37.0 35.0 37.0 26.0 37.0 6 33.057689486589815 37.0 35.0 37.0 26.0 37.0 7 32.91623408366623 37.0 35.0 37.0 26.0 37.0 8 32.85550530700443 37.0 35.0 37.0 26.0 37.0 9 34.2221994413313 39.0 35.0 39.0 25.0 39.0 10 34.122248935356104 39.0 35.0 39.0 25.0 39.0 11 34.16996779188307 39.0 35.0 39.0 25.0 39.0 12 34.07829414947544 39.0 35.0 39.0 25.0 39.0 13 34.06856119520452 39.0 35.0 39.0 25.0 39.0 14 35.089040925943095 40.0 35.0 41.0 24.0 41.0 15 35.02430169677009 40.0 35.0 41.0 24.0 41.0 16 34.9163159207521 39.0 35.0 41.0 22.0 41.0 17 34.8589920892268 39.0 35.0 41.0 22.0 41.0 18 34.56284457004406 39.0 34.0 41.0 18.0 41.0 19 34.801670434219744 39.0 34.0 41.0 19.0 41.0 20 34.87265801195953 40.0 35.0 41.0 22.0 41.0 21 34.81244463481724 40.0 35.0 41.0 20.0 41.0 22 34.7224530057064 39.0 35.0 41.0 19.0 41.0 23 34.46370372885458 39.0 34.0 41.0 18.0 41.0 24 34.46327364885013 39.0 34.0 41.0 18.0 41.0 25 34.38380635604269 39.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.553145933573262 1101 2 0.8066354897053536 1101 3 0.8967772819862638 1101 4 0.7723755103885566 1101 5 0.6675230754078356 1101 6 0.7679760063274585 1101 7 0.8339858293312901 1101 8 0.6317979042957518 1101 9 0.6227947219625705 1101 10 0.7201687092468632 1101 11 0.850569656426643 1101 12 0.9972081361452823 1101 13 0.8909680805897437 1101 14 0.9880613387862311 1101 15 0.745005352811063 1101 16 1.1456753413658305 1101 17 1.1931952704534652 1101 18 1.3289301062102297 1101 19 0.839961394085833 1101 20 0.9735291674258306 1101 21 0.936510315168249 1101 22 1.0080862956843362 1101 23 1.2045740000455183 1101 24 0.9061840472315055 1101 25 1.1087597880276334 1102 1 0.673288406081209 1102 2 0.8056907611368374 1102 3 0.807507166579299 1102 4 0.9460105924638569 1102 5 0.6871609871792899 1102 6 0.7468635996619 1102 7 0.9028102355248109 1102 8 0.8575639810361864 1102 9 0.7729379384522872 1102 10 0.9131813094759593 1102 11 0.9255421739968028 1102 12 0.9778075289022325 1102 13 0.9279141508908921 1102 14 1.0183516584290189 1102 15 1.1559946689763763 1102 16 0.9938581659054222 1102 17 0.9210679059062485 1102 18 1.2011797787941205 1102 19 1.2455279810951367 1102 20 1.1332655798174898 1102 21 0.9293578284092021 1102 22 1.1952826901947802 1102 23 1.3703212720054907 1102 24 1.255278890796511 1102 25 1.1854999036526621 1103 1 0.28237501907549856 1103 2 0.4685619942186534 1103 3 0.6087774925750509 1103 4 0.6218393066662387 1103 5 0.19721098920682323 1103 6 0.505669165701697 1103 7 0.4097462622507848 1103 8 0.4301849856308806 1103 9 0.7049623187297129 1103 10 0.6713985796709423 1103 11 0.665885045042728 1103 12 0.7428791007376461 1103 13 0.6876505095080532 1103 14 0.8655937487196397 1103 15 0.9340836120713831 1103 16 0.9980969528805659 1103 17 0.8660371342280442 1103 18 1.1927401040724348 1103 19 1.1853131252213842 1103 20 1.076895078206384 1103 21 0.9366503886190714 1103 22 1.1167186810490506 1103 23 1.3983303615817988 1103 24 1.223422015751673 1103 25 1.1490987083453064 1104 1 0.6243980467205255 1104 2 0.5996219139705659 1104 3 0.6244200968740898 1104 4 0.8629547904830801 1104 5 0.6477200250059667 1104 6 0.55226200864373 1104 7 0.8578210701549125 1104 8 0.7536424266667012 1104 9 0.8921770420138984 1104 10 0.9435764462540774 1104 11 0.9335408942015704 1104 12 0.8813647779242118 1104 13 0.8320822112739563 1104 14 1.139197777595406 1104 15 1.1689962250910284 1104 16 0.943509857998329 1104 17 1.1508529584796534 1104 18 1.2214274482579128 1104 19 1.209759158652183 1104 20 1.16245727278298 1104 21 1.2011252547119042 1104 22 1.2022197620814552 1104 23 1.2706463109083543 1104 24 1.1178681492424332 1104 25 0.882024823301883 1105 1 0.6628189576602566 1105 2 0.7598278700211054 1105 3 0.7746732249289145 1105 4 0.7629683979405257 1105 5 0.552922074125334 1105 6 0.3914613248419698 1105 7 0.6603004848210929 1105 8 0.7401528953733347 1105 9 0.7995792208443078 1105 10 0.916814624176908 1105 11 0.8787591394388414 1105 12 0.9861554833357289 1105 13 0.8793258825319938 1105 14 1.0724413830557538 1105 15 1.2172336577700307 1105 16 1.2534275012175868 1105 17 1.142275240513591 1105 18 1.1859665840623492 1105 19 1.1054896605634852 1105 20 1.2828491366663926 1105 21 1.1850109205606287 1105 22 0.9145816660813821 1105 23 1.1998864510234668 1105 24 1.2130424289546085 1105 25 1.2363029322390773 1106 1 0.7143642575197546 1106 2 0.8365478234560548 1106 3 0.8505939769033617 1106 4 0.8864047286860028 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4 -1.5400442345575414 2214 5 -1.663602965494647 2214 6 -1.6922239221917081 2214 7 -1.9706801759891768 2214 8 -1.6641980801051908 2214 9 -1.764782859985452 2214 10 -1.8165795663523312 2214 11 -1.6153509661892187 2214 12 -1.995916041503392 2214 13 -1.7884629141719586 2214 14 -2.9252206313126052 2214 15 -3.152006065311209 2214 16 -2.294370358844361 2214 17 -2.734048975191108 2214 18 -2.1596329607728535 2214 19 -4.1592656327967035 2214 20 -2.537588477944105 2214 21 -2.4840870546935676 2214 22 -2.5990045136826225 2214 23 -2.66480814618129 2214 24 -2.8128893552659697 2214 25 -2.680729658649348 2215 1 -1.0336238258769122 2215 2 -2.6077402262167553 2215 3 -1.4397061086208396 2215 4 -1.0142797015243872 2215 5 -0.961342903973609 2215 6 -1.5029289192166395 2215 7 -1.9068503676904704 2215 8 -1.7393361163228072 2215 9 -1.5367127236273532 2215 10 -3.0664865026660806 2215 11 -2.018846151133495 2215 12 -2.3094928947825544 2215 13 -1.61733661585928 2215 14 -2.9830154080637072 2215 15 -2.8217185876242183 2215 16 -2.4359397789090877 2215 17 -2.7764496321811265 2215 18 -1.6381374768367323 2215 19 -2.27344066776984 2215 20 -2.403045861211055 2215 21 -2.065494266733239 2215 22 -1.6720397066066113 2215 23 -2.285975828133175 2215 24 -2.1505169836963347 2215 25 -2.055615927894472 2216 1 -1.8934871809610243 2216 2 -2.0263283080399184 2216 3 -2.5385104103888665 2216 4 -3.1818486296808928 2216 5 -2.440314884850867 2216 6 -2.383043589082888 2216 7 -2.1498607082306833 2216 8 -2.7685870243609436 2216 9 -2.9543845270935414 2216 10 -2.6181150417551287 2216 11 -3.2548034589843304 2216 12 -3.5032398938987264 2216 13 -4.261959592715652 2216 14 -3.431472329388818 2216 15 -3.393779556408912 2216 16 -2.5014723705887434 2216 17 -2.6083224079591645 2216 18 -2.653907981474532 2216 19 -3.046399276725751 2216 20 -2.8495388920247144 2216 21 -3.165820630370529 2216 22 -3.718936576104994 2216 23 -3.742603988342058 2216 24 -3.4999601997535095 2216 25 -3.6602145476832995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104330.0 3 5.0 4 899.0 5 5302.0 6 6408.0 7 5780.0 8 5117.0 9 4937.0 10 4511.0 11 4234.0 12 4169.0 13 4157.0 14 4170.0 15 4150.0 16 4415.0 17 4632.0 18 5320.0 19 6163.0 20 8159.0 21 10325.0 22 12647.0 23 15710.0 24 17807.0 25 20351.0 26 22707.0 27 25567.0 28 28871.0 29 34387.0 30 41935.0 31 53128.0 32 66811.0 33 85258.0 34 111732.0 35 146332.0 36 201411.0 37 313158.0 38 902252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.234722086309816 18.643667957446215 12.576057553856263 25.54555240238771 2 15.041080392326784 20.177795119300143 40.9103834736066 23.87074101476647 3 18.299897668139955 24.680702823923884 29.917916784602323 27.10148272333384 4 12.240491887513825 16.867221723870653 38.587260392081724 32.3050259965338 5 13.111155336326231 37.74244695986243 36.18513391167721 12.961263792134133 6 31.576039490675473 37.364803735209065 18.294043222749806 12.765113551365657 7 27.562301603369132 31.693218236620485 22.83966178041153 17.90481837959885 8 24.9668222508621 36.51990664948274 20.983097634888015 17.530173464767145 9 26.33623681091752 15.142072443841089 20.27938170991986 38.24230903532153 10 14.58531163180725 28.84817421827005 35.07758848518176 21.48892566474094 11 34.12036385975561 22.52293280402156 24.771608037028603 18.585095299194236 12 22.634636057162936 24.76895058043724 32.10240495157834 20.494008410821476 13 28.599890543317024 21.88275944042121 25.952989356055973 23.56436066020579 14 21.007263222474233 21.689651704077132 27.944356949437854 29.35872812401078 15 23.092799306108304 30.106565671519615 23.623929510078323 23.17670551229376 16 22.6283909518307 28.02038859722376 27.039969975278368 22.311250475667173 17 21.868799654467818 28.21699560291142 27.655244569819835 22.258960172800922 18 21.76858654256756 27.743296121031484 28.90511298770338 21.583004348697568 19 23.20268595122287 27.20487867109681 27.60954782336849 21.982887554311834 20 23.338008060966228 27.3047053071148 27.843293144608438 21.513993487310533 21 23.232375569846777 26.8457375243287 27.17264561299621 22.749241292828312 22 23.124521072796934 27.58912399860676 27.305686172065478 21.980668756530825 23 22.601561166590617 27.36108160073565 27.407886991323373 22.629470241350365 24 22.84755568462295 26.96677448244725 27.874203409149782 22.31146642378002 25 22.93411252165561 27.094906757333952 27.572015923376913 22.398964797633525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 275.0 1 275.0 2 441.5 3 608.0 4 608.0 5 608.0 6 1246.5 7 1885.0 8 1885.0 9 1885.0 10 3018.0 11 4151.0 12 4151.0 13 4151.0 14 7473.5 15 10796.0 16 10796.0 17 10796.0 18 19318.0 19 27840.0 20 27840.0 21 27840.0 22 43122.0 23 58404.0 24 58404.0 25 58404.0 26 82212.5 27 106021.0 28 106021.0 29 106021.0 30 135823.5 31 165626.0 32 165626.0 33 165626.0 34 190176.0 35 214726.0 36 214726.0 37 214726.0 38 235343.5 39 255961.0 40 255961.0 41 255961.0 42 270600.5 43 285240.0 44 285240.0 45 285240.0 46 291115.5 47 296991.0 48 296991.0 49 296991.0 50 287739.0 51 278487.0 52 278487.0 53 278487.0 54 251931.0 55 225375.0 56 225375.0 57 225375.0 58 194838.0 59 164301.0 60 164301.0 61 164301.0 62 133816.0 63 103331.0 64 103331.0 65 103331.0 66 80017.5 67 56704.0 68 56704.0 69 56704.0 70 41225.5 71 25747.0 72 25747.0 73 25747.0 74 17767.0 75 9787.0 76 9787.0 77 9787.0 78 6636.5 79 3486.0 80 3486.0 81 3486.0 82 2271.5 83 1057.0 84 1057.0 85 1057.0 86 697.5 87 338.0 88 338.0 89 338.0 90 201.0 91 64.0 92 64.0 93 64.0 94 43.5 95 23.0 96 23.0 97 23.0 98 23.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3565572182703906 2 0.03900320688197656 3 0.03469370076443674 4 0.03425839711620039 5 0.04004793563774378 6 0.017455676294277456 7 0.01723802447015928 8 0.022940502262055407 9 0.020067498183695527 10 0.019588664170635546 11 0.018630996144515587 12 0.01932748198169374 13 0.01950160344098828 14 0.026466461812769806 15 0.02764178166300794 16 0.02058986256157914 17 0.021765182411817276 18 0.1004245516481249 19 0.02420288284194081 20 0.021765182411817276 21 0.025117020503237133 22 0.019458073076164643 23 0.02189577350628818 24 0.018630996144515587 25 0.021678121682170006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2297247.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.40833897358104 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63753385766027 59.11238224532664 2 12.293646892784789 17.803251028685448 3 3.3554418523301077 7.288859131489773 4 1.2177426030511187 3.5269887673718547 5 0.5337311056956512 1.932329141097746 6 0.28937400711207373 1.2571854718268691 7 0.16478184965181492 0.8352106018397597 8 0.10127814066277917 0.586670555177963 9 0.0724515219882147 0.472148492694706 >10 0.3085527615359893 3.7511223198752686 >50 0.014917012281583632 0.7351364855082828 >100 0.008983499938996607 1.3590255351107667 >500 0.0010833890584675925 0.6012045100849781 >1k 4.81506248207786E-4 0.7384857139100696 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3501 0.15239980724754457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.353036482363455E-5 2 0.0 0.0 0.0 0.0 4.353036482363455E-5 3 0.0 0.0 0.0 0.0 4.353036482363455E-5 4 0.0 0.0 0.0 4.353036482363455E-5 4.353036482363455E-5 5 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 6 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 7 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 8 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 9 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 10 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 11 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 12 0.0 0.0 0.0 4.353036482363455E-5 8.70607296472691E-5 13 0.0 0.0 0.0 4.353036482363455E-5 1.3059109447090364E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 100 1.4398756E-7 12.352435 18 TAGGACC 550 0.0 12.260979 4 GGTATCA 965 0.0 12.14958 1 CGTGCGC 90 7.510069E-6 11.604805 10 GTCCTAC 1325 0.0 10.790928 1 CGGAACT 80 3.7797095E-4 10.6851845 4 GTAGGAC 1340 0.0 10.632025 3 GGCGAGG 590 0.0 10.459963 19 CTTATAC 195 1.4551915E-11 10.228553 3 AGGACGT 1290 0.0 10.160589 5 GGACGTG 1270 0.0 10.092281 6 GGACCGT 85 6.6325E-4 10.052704 6 GTTCTAA 295 0.0 10.016665 1 GTATCAA 2075 0.0 9.968391 1 TGGACTG 375 0.0 9.877859 5 TCCTACA 1485 0.0 9.849718 2 GTAGAAC 405 0.0 9.849717 3 TGTAGGA 1515 0.0 9.8427515 2 CTTAGAC 195 1.6370905E-10 9.741479 3 AGGACCT 1085 0.0 9.716789 5 >>END_MODULE