FastQCFastQC Report
Thu 26 May 2016
SRR1512928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297247
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39340.17124845521617832No Hit
TATCAACGCAGAGTACTTTTTTTTT29330.12767456002772012No Hit
GGTATCAACGCAGAGTACTTTTTTT24480.10656233308825738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1155.298716E-912.3960688
GGTATCA9600.011.7826221
TAGGACC5050.011.4929374
CCGCTCG600.005872692411.0844529
GTACTAG1659.2040864E-1010.3694281
TGGCGAG12650.010.27792918
GGCGAGG5650.010.24673119
GTATCAA18550.010.197071
GCGTGCG1054.1019757E-59.9533859
CGTGCGC1054.1049236E-59.95273510
GTAATAC1356.3774314E-79.8595083
GTCCTAA7300.09.7657291
AGGACCT10550.09.7401095
GGCGAGA7750.09.67451819
TATGGCG13850.09.59549216
GTCCTAC11800.09.5858621
ATGGCGA13850.09.52695317
AACACCG1201.670227E-59.5146445
TTAGGAC9500.09.4073053
TAATCCC3350.09.3726355
Produced by FastQC (version 0.11.5)