##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512928_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297247 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.46984803984944 31.0 31.0 34.0 27.0 34.0 2 30.622165574707466 31.0 31.0 34.0 27.0 34.0 3 30.705453962939117 31.0 31.0 34.0 27.0 34.0 4 34.186960305095624 37.0 35.0 37.0 30.0 37.0 5 33.82989682868233 37.0 35.0 37.0 29.0 37.0 6 33.68407097713045 37.0 35.0 37.0 29.0 37.0 7 33.5666225704071 37.0 35.0 37.0 28.0 37.0 8 33.45327211222825 37.0 35.0 37.0 28.0 37.0 9 34.917168680599 39.0 35.0 39.0 28.0 39.0 10 34.81074085633804 39.0 35.0 39.0 27.0 39.0 11 34.82669821747509 39.0 35.0 39.0 27.0 39.0 12 34.68620135318492 39.0 35.0 39.0 27.0 39.0 13 34.65959951193755 39.0 35.0 39.0 27.0 39.0 14 35.73061625502177 40.0 36.0 41.0 27.0 41.0 15 35.738260187084805 40.0 36.0 41.0 27.0 41.0 16 35.71070372493685 40.0 36.0 41.0 27.0 41.0 17 35.685042574873314 40.0 36.0 41.0 27.0 41.0 18 35.60798991140265 40.0 36.0 41.0 27.0 41.0 19 35.59616532310196 40.0 36.0 41.0 27.0 41.0 20 35.564620173625215 40.0 36.0 41.0 26.0 41.0 21 35.51459812549543 40.0 36.0 41.0 26.0 41.0 22 35.471679362297564 40.0 36.0 41.0 26.0 41.0 23 35.36405358239667 40.0 36.0 41.0 25.0 41.0 24 35.34270411496892 40.0 36.0 41.0 25.0 41.0 25 35.24544944448725 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5678562528570446 1101 2 0.6078076505003196 1101 3 0.6422709424770794 1101 4 0.41281941476047734 1101 5 0.5512064480307473 1101 6 0.686991079990527 1101 7 0.5819580517491261 1101 8 0.6171136316476478 1101 9 0.422982801302517 1101 10 0.7874574456057246 1101 11 0.8499340620818074 1101 12 0.8416133948238311 1101 13 0.804469610017982 1101 14 1.036835214961684 1101 15 0.8887672603663503 1101 16 1.1319592872612816 1101 17 0.8699578587560808 1101 18 0.5162137987252677 1101 19 0.8327829965782172 1101 20 0.8138791258052294 1101 21 1.0243262663327926 1101 22 0.8812822442959032 1101 23 0.8276547261273492 1101 24 0.8465841480544469 1101 25 0.8991665629585839 1102 1 0.5463529350831919 1102 2 0.5372271907965533 1102 3 0.5366864614236029 1102 4 0.4449793092689944 1102 5 0.6930359889811726 1102 6 0.545174686993704 1102 7 0.5038386837231528 1102 8 0.580315074972269 1102 9 0.7719240382876436 1102 10 0.8447857301248689 1102 11 0.7883939428651416 1102 12 0.7805831596614539 1102 13 0.9699920840395464 1102 14 1.0757598919389153 1102 15 1.0254787198661646 1102 16 0.949210107885996 1102 17 0.9768022985827045 1102 18 0.9723050852878004 1102 19 1.0340460980329524 1102 20 0.9511867327622312 1102 21 0.9801895481389735 1102 22 1.0523621338274438 1102 23 1.0337367129178219 1102 24 1.1332056883788937 1102 25 1.1408204985579857 1103 1 0.6098570696862815 1103 2 0.46464221386361615 1103 3 0.569219128063235 1103 4 0.5624773675183974 1103 5 0.62787177458695 1103 6 0.7273821688940956 1103 7 0.5873823083866583 1103 8 0.6409452627216154 1103 9 0.7909179463375651 1103 10 0.7890177170738113 1103 11 0.7180451763836544 1103 12 0.8457916678043347 1103 13 0.827708242211898 1103 14 0.9256925155325106 1103 15 0.8516734813505664 1103 16 0.7904187345614417 1103 17 0.9416414842015826 1103 18 0.9977858440210028 1103 19 1.0285815907385256 1103 20 1.0913907959338474 1103 21 0.7963098212629589 1103 22 1.0173530723246316 1103 23 1.0566875811688732 1103 24 1.1115061185273447 1103 25 1.1616819183909826 1104 1 0.4305605527734535 1104 2 0.540063100687334 1104 3 0.4018462176807809 1104 4 0.43083248185229195 1104 5 0.6329801433710145 1104 6 0.5805108513892208 1104 7 0.6450888109755155 1104 8 0.6101902949370768 1104 9 0.7075852415860311 1104 10 0.8889241225275413 1104 11 0.8559394992124894 1104 12 0.7831718363822731 1104 13 0.8207250576728597 1104 14 0.9636069273223455 1104 15 0.8945469416779446 1104 16 0.8511494339574597 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0.5108432473991158 1107 17 0.48108601655830796 1107 18 0.5902405727720819 1107 19 0.8143897725367424 1107 20 0.7373995742063713 1107 21 0.6876323996855547 1107 22 0.759452967763707 1107 23 0.6955276135441508 1107 24 0.730387051565188 1107 25 0.8308705906791545 1108 1 0.38474265103926797 1108 2 0.3633724135554637 1108 3 0.31409552556462117 1108 4 0.3760747808600229 1108 5 0.34380689560886424 1108 6 0.383295127232806 1108 7 0.26027704323140455 1108 8 0.4054272859013537 1108 9 0.35194881470489037 1108 10 0.40799641205609305 1108 11 0.3929034338806403 1108 12 0.4018313784750589 1108 13 0.34961210777852614 1108 14 0.48507408559163423 1108 15 0.3460333820738839 1108 16 0.3676784554027108 1108 17 0.4121901624100488 1108 18 0.4726175366579852 1108 19 0.39540663457054137 1108 20 0.3789280816123508 1108 21 0.5615918262267954 1108 22 0.522315388173233 1108 23 0.414126880463769 1108 24 0.45281902980830324 1108 25 0.44211424251263054 1109 1 0.19692473932385468 1109 2 0.17259228778868874 1109 3 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2216 18 -2.0519155662545856 2216 19 -2.300702347555415 2216 20 -2.560001011461523 2216 21 -2.521215044687729 2216 22 -2.8337384513960373 2216 23 -1.9215373076708033 2216 24 -2.3235629491967913 2216 25 -2.2207338034508126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59223.0 3 1.0 4 980.0 5 8530.0 6 9862.0 7 7665.0 8 6169.0 9 5566.0 10 4782.0 11 4384.0 12 4049.0 13 3823.0 14 3602.0 15 3674.0 16 3866.0 17 4048.0 18 4327.0 19 5241.0 20 6770.0 21 8711.0 22 10783.0 23 13235.0 24 15721.0 25 18239.0 26 20644.0 27 23008.0 28 25701.0 29 30875.0 30 38744.0 31 49826.0 32 62790.0 33 81804.0 34 106436.0 35 139411.0 36 192941.0 37 298840.0 38 1012976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.41851265822785 19.39410758099593 13.47968828416885 27.707691476607373 2 13.678863160002525 20.623834201402115 39.75074491401657 25.94655772457879 3 16.585417240723352 24.709924531406504 30.412924687680515 28.29173354018963 4 11.4092212193941 17.408918164812924 37.91382625567357 33.26803436011941 5 12.752697905581037 37.98130056190666 36.19682546043979 13.06917607207251 6 31.619434803387048 37.76773084481511 17.92677598151821 12.686058370279627 7 27.35054719383534 31.79311143663856 22.773035179608126 18.083306189917977 8 24.90256848084366 36.77189895087152 20.661231903785968 17.66430066449886 9 26.003884712870946 14.965595031748382 20.057399681209663 38.97312057417102 10 14.21378922970531 29.12984688752942 35.09389942431942 21.56246445844585 11 34.093317209033444 22.43066855770227 24.66682686489456 18.80918736836973 12 22.448147954652317 24.862032635006877 32.004989290005746 20.684830120335057 13 28.456022490908122 21.83068608936636 26.030797011544095 23.682494408181427 14 20.851719058000796 21.603719865985916 27.855678881637147 29.68888219437614 15 22.86132585476876 30.233159516493735 23.59243622483407 23.313078403903432 16 22.49902584185767 28.08270439361649 26.95181019263297 22.466459571892873 17 21.788695413634997 28.28680783248207 27.642591842688507 22.281904911194424 18 21.73203866251787 27.838440683750566 28.722642925424385 21.706877728307184 19 23.095852830259318 27.2043225604519 27.60742953137291 22.092395077915878 20 23.304334728412336 27.253188990397547 27.777801962629763 21.664674318560355 21 22.96530103027802 26.77699887343269 27.281429399750312 22.976270696538982 22 22.932327717736946 27.504132145063213 27.353211450471065 22.210328686728772 23 22.42849811099982 27.34163979754898 27.464961321094332 22.764900770356867 24 22.713893070224085 26.987401436328547 27.92269526206597 22.3760102313814 25 22.71427433365968 27.055809035333983 27.634007027007968 22.59590960399837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 172.0 2 328.5 3 485.0 4 485.0 5 485.0 6 1201.0 7 1917.0 8 1917.0 9 1917.0 10 2938.0 11 3959.0 12 3959.0 13 3959.0 14 7421.5 15 10884.0 16 10884.0 17 10884.0 18 19206.0 19 27528.0 20 27528.0 21 27528.0 22 43586.0 23 59644.0 24 59644.0 25 59644.0 26 83266.0 27 106888.0 28 106888.0 29 106888.0 30 135760.5 31 164633.0 32 164633.0 33 164633.0 34 189526.5 35 214420.0 36 214420.0 37 214420.0 38 235740.5 39 257061.0 40 257061.0 41 257061.0 42 270849.0 43 284637.0 44 284637.0 45 284637.0 46 291269.5 47 297902.0 48 297902.0 49 297902.0 50 289175.5 51 280449.0 52 280449.0 53 280449.0 54 253441.5 55 226434.0 56 226434.0 57 226434.0 58 194834.5 59 163235.0 60 163235.0 61 163235.0 62 132672.5 63 102110.0 64 102110.0 65 102110.0 66 78889.0 67 55668.0 68 55668.0 69 55668.0 70 40334.0 71 25000.0 72 25000.0 73 25000.0 74 17278.5 75 9557.0 76 9557.0 77 9557.0 78 6461.0 79 3365.0 80 3365.0 81 3365.0 82 2156.5 83 948.0 84 948.0 85 948.0 86 624.0 87 300.0 88 300.0 89 300.0 90 171.0 91 42.0 92 42.0 93 42.0 94 25.5 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07935585507348579 2 8.70607296472691E-5 3 0.0 4 0.0274676602037134 5 0.0726957092554697 6 0.07826759595289491 7 0.03495488295337854 8 0.06668851890980813 9 0.04644689926681807 10 0.10438581484707565 11 0.004048323928598013 12 0.014234429297328498 13 0.006137781440132471 14 0.007617813844136047 15 0.008401360410961468 16 0.017063903010864745 17 0.028599449689127903 18 0.0015235627688272092 19 0.0039177328341271096 20 0.005441295602954319 21 1.3059109447090364E-4 22 6.094251075308837E-4 23 0.0 24 4.353036482363455E-5 25 0.0047883401305998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2297247.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.5487992766046 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84535623525124 57.84388165721586 2 12.927409517721022 18.498812574997785 3 3.4002869592116256 7.298593473824664 4 1.2529504599687808 3.585884038553428 5 0.5808846983563619 2.0780801342775175 6 0.2776619422069061 1.1919827141828467 7 0.17669327270822896 0.8849534051769201 8 0.10976116664727265 0.6282623744649326 9 0.0717485832715073 0.46201724845864434 >10 0.33177121684111216 3.964247200685612 >50 0.014457186838005802 0.7164623760987545 >100 0.009255623881898697 1.3545947544116124 >500 0.0010943609561634 0.5372264572218126 >1k 6.687761398745816E-4 0.9550015904296758 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3934 0.17124845521617832 No Hit TATCAACGCAGAGTACTTTTTTTTT 2933 0.12767456002772012 No Hit GGTATCAACGCAGAGTACTTTTTTT 2448 0.10656233308825738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.353036482363455E-5 0.0 0.0 0.0 4 0.0 4.353036482363455E-5 0.0 0.0 0.0 5 0.0 4.353036482363455E-5 0.0 0.0 0.0 6 0.0 4.353036482363455E-5 0.0 0.0 0.0 7 0.0 8.70607296472691E-5 0.0 0.0 0.0 8 0.0 8.70607296472691E-5 0.0 0.0 0.0 9 0.0 8.70607296472691E-5 0.0 4.353036482363455E-5 0.0 10 0.0 1.3059109447090364E-4 0.0 8.70607296472691E-5 0.0 11 0.0 1.3059109447090364E-4 0.0 8.70607296472691E-5 0.0 12 0.0 1.3059109447090364E-4 0.0 8.70607296472691E-5 4.353036482363455E-5 13 0.0 1.3059109447090364E-4 0.0 8.70607296472691E-5 4.353036482363455E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGC 115 5.298716E-9 12.396068 8 GGTATCA 960 0.0 11.782622 1 TAGGACC 505 0.0 11.492937 4 CCGCTCG 60 0.0058726924 11.084452 9 GTACTAG 165 9.2040864E-10 10.369428 1 TGGCGAG 1265 0.0 10.277929 18 GGCGAGG 565 0.0 10.246731 19 GTATCAA 1855 0.0 10.19707 1 GCGTGCG 105 4.1019757E-5 9.953385 9 CGTGCGC 105 4.1049236E-5 9.952735 10 GTAATAC 135 6.3774314E-7 9.859508 3 GTCCTAA 730 0.0 9.765729 1 AGGACCT 1055 0.0 9.740109 5 GGCGAGA 775 0.0 9.674518 19 TATGGCG 1385 0.0 9.595492 16 GTCCTAC 1180 0.0 9.585862 1 ATGGCGA 1385 0.0 9.526953 17 AACACCG 120 1.670227E-5 9.514644 5 TTAGGAC 950 0.0 9.407305 3 TAATCCC 335 0.0 9.372635 5 >>END_MODULE