FastQCFastQC Report
Thu 26 May 2016
SRR1512927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570590
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44480.1730342061550072No Hit
GGTATCAACGCAGAGTACTTTTTTT26290.10227224100303821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6150.013.7441654
GGTATCA10750.012.4102091
GTAGGAC14650.011.9284413
GTCCTAC14700.011.6684891
TGTAGGA15900.011.3492822
GTACCGT855.3566866E-511.170126
GTGTAGG5150.011.1020581
GTCCTAG5100.010.8372061
AGGACCT13400.010.6311815
TCCTACA16450.010.5656722
GGACGTG10850.010.4134346
TACACCG1201.530485E-610.288625
AGGACGT10900.010.281365
GTATCAA22000.010.265621
GATATAC4750.010.2314241
TGTACCG1303.8597682E-710.2277415
TAGGACA7450.010.1985224
GTATTAA3850.010.148051
TAGGACG11800.010.1412624
AATACGG750.002655089410.1303345
Produced by FastQC (version 0.11.5)