##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512927_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2570590 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.766106224641035 31.0 30.0 34.0 22.0 34.0 2 28.879382943215372 31.0 30.0 34.0 16.0 34.0 3 29.093156045888296 31.0 30.0 34.0 22.0 34.0 4 32.50220416324657 35.0 33.0 37.0 24.0 37.0 5 32.63713194247235 35.0 33.0 37.0 25.0 37.0 6 32.64664376660611 37.0 33.0 37.0 25.0 37.0 7 32.4883380858091 37.0 33.0 37.0 23.0 37.0 8 32.42959320622892 37.0 33.0 37.0 23.0 37.0 9 33.747213285665936 38.0 34.0 39.0 22.0 39.0 10 33.64261667554919 38.0 34.0 39.0 19.0 39.0 11 33.683571864824806 38.0 34.0 39.0 22.0 39.0 12 33.571596792954146 38.0 34.0 39.0 19.0 39.0 13 33.56107663999315 38.0 34.0 39.0 18.0 39.0 14 34.55302557000533 39.0 34.0 41.0 18.0 41.0 15 34.48227294123139 39.0 34.0 41.0 17.0 41.0 16 34.371459081378205 39.0 34.0 41.0 17.0 41.0 17 34.30935310570725 39.0 34.0 41.0 17.0 41.0 18 34.004163635585606 39.0 33.0 41.0 11.0 41.0 19 34.239072353039575 39.0 34.0 41.0 18.0 41.0 20 34.308568849952735 39.0 34.0 41.0 17.0 41.0 21 34.243764272015376 39.0 34.0 41.0 16.0 41.0 22 34.15456062615975 39.0 34.0 41.0 10.0 41.0 23 33.889663851489345 39.0 34.0 41.0 2.0 41.0 24 33.88687655363166 39.0 34.0 41.0 2.0 41.0 25 33.80004084665388 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5178473759040756 1101 2 0.6782293147487515 1101 3 0.7291620100388201 1101 4 0.7481796609512443 1101 5 0.5687523794362193 1101 6 0.77644746041328 1101 7 0.8388767717771728 1101 8 0.6128097967117725 1101 9 0.520700102991384 1101 10 0.6372004488765768 1101 11 0.7311575571600102 1101 12 1.0075071432785236 1101 13 0.7784599753679871 1101 14 0.6902820648564543 1101 15 0.4818726208480797 1101 16 0.9713053026892169 1101 17 1.0219134366962237 1101 18 1.2087115834679096 1101 19 0.6161260512754225 1101 20 0.7997210958366736 1101 21 0.7332619090276253 1101 22 0.7577129535412794 1101 23 0.9029355172851297 1101 24 0.5944341588629456 1101 25 0.79356533524615 1102 1 0.7978126592350847 1102 2 0.9065567363331901 1102 3 0.8548809445793566 1102 4 1.0508327662493073 1102 5 0.9184038247926054 1102 6 0.8932981986009025 1102 7 1.1176437672063528 1102 8 1.1232896746940852 1102 9 1.0690399193842097 1102 10 1.2533913360565734 1102 11 1.2373435648551308 1102 12 1.2839775210362205 1102 13 1.1917275137544578 1102 14 1.2276172549067894 1102 15 1.3499958833592771 1102 16 1.2627012759427174 1102 17 1.190435143782473 1102 18 1.5579118165422585 1102 19 1.526869296652393 1102 20 1.4270940509606973 1102 21 1.13735870262542 1102 22 1.525228674345101 1102 23 1.6742232887255781 1102 24 1.6563167741445994 1102 25 1.4909560332569711 1103 1 0.44551859052602083 1103 2 0.5744283511866328 1103 3 0.6516452127481998 1103 4 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-4.151027522327279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138879.0 3 0.0 4 1211.0 5 6604.0 6 7891.0 7 7458.0 8 6524.0 9 6170.0 10 5691.0 11 5371.0 12 5238.0 13 4986.0 14 5077.0 15 5242.0 16 5288.0 17 5574.0 18 6286.0 19 7487.0 20 9842.0 21 12327.0 22 15219.0 23 18322.0 24 21228.0 25 23961.0 26 27071.0 27 30161.0 28 33937.0 29 40106.0 30 49060.0 31 61204.0 32 77302.0 33 97591.0 34 125468.0 35 165042.0 36 223347.0 37 345677.0 38 962748.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47323021209463 17.78398259424053 12.323991362708089 25.418795830956753 2 15.417743559367613 19.073464287118114 40.85943334655247 24.649358806961803 3 19.620783215520476 23.145945769244538 28.85034783461472 28.382923180620267 4 12.585275188335093 15.558591886277437 37.182317087882964 34.673815837504506 5 13.612989674592631 37.4354491772093 35.348806979759 13.602754168439068 6 33.634654136037526 36.091845973490045 17.0632680305152 13.210231859957226 7 29.16672827175548 31.049509480828625 21.05026430528525 18.733497942130644 8 25.741922404813423 36.86232455595941 19.525568107754964 17.870184931472203 9 26.206457813323787 15.450192893954043 19.315425874442866 39.0279234182793 10 15.102901906524687 28.646701703339485 33.74026144823349 22.510134941902336 11 35.607561253381675 22.200977937460095 23.368752147287484 18.82270866187075 12 23.431430722569274 24.23218868005284 30.911747368257224 21.424633229120666 13 29.247129748555583 21.17883124629631 25.395134998216 24.178904006932104 14 21.33966108448606 21.12252728181166 26.81851505383206 30.71929657987022 15 23.48257239015837 29.761010234413636 22.838646011318133 23.91777136410986 16 23.24084176252784 27.879103639015174 26.016717275555674 22.863337322901316 17 22.362671526836902 28.10187991779007 26.885540345041996 22.649908210331027 18 22.452241329757776 27.381585680538173 28.12011499311085 22.046057996593202 19 23.816620485464888 26.771893138735436 26.826407839221712 22.585078536577964 20 23.832123349128366 26.900832138135083 27.27281570548862 21.99422880724792 21 23.598642603132628 26.375772932921233 26.687533731607882 23.338050732338257 22 23.515326583600412 27.080317048866632 26.817562275371515 22.58679409216144 23 23.17351664800913 26.91506287726065 26.904595979699664 23.00682449503056 24 23.419196366212848 26.404565423693466 27.330326707004897 22.84591150308879 25 23.243237774765255 26.75512799162027 27.07384245676674 22.927791776847734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 195.0 2 413.5 3 632.0 4 632.0 5 632.0 6 1325.5 7 2019.0 8 2019.0 9 2019.0 10 3160.0 11 4301.0 12 4301.0 13 4301.0 14 7183.5 15 10066.0 16 10066.0 17 10066.0 18 17700.5 19 25335.0 20 25335.0 21 25335.0 22 39489.5 23 53644.0 24 53644.0 25 53644.0 26 75803.5 27 97963.0 28 97963.0 29 97963.0 30 128423.0 31 158883.0 32 158883.0 33 158883.0 34 185588.5 35 212294.0 36 212294.0 37 212294.0 38 237874.5 39 263455.0 40 263455.0 41 263455.0 42 285291.5 43 307128.0 44 307128.0 45 307128.0 46 321893.5 47 336659.0 48 336659.0 49 336659.0 50 333802.0 51 330945.0 52 330945.0 53 330945.0 54 306075.5 55 281206.0 56 281206.0 57 281206.0 58 246200.5 59 211195.0 60 211195.0 61 211195.0 62 175009.5 63 138824.0 64 138824.0 65 138824.0 66 108157.0 67 77490.0 68 77490.0 69 77490.0 70 56933.5 71 36377.0 72 36377.0 73 36377.0 74 25503.0 75 14629.0 76 14629.0 77 14629.0 78 9890.5 79 5152.0 80 5152.0 81 5152.0 82 3364.5 83 1577.0 84 1577.0 85 1577.0 86 1018.0 87 459.0 88 459.0 89 459.0 90 288.5 91 118.0 92 118.0 93 118.0 94 70.0 95 22.0 96 22.0 97 22.0 98 22.0 99 22.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3555214950653352 2 0.04213040586013328 3 0.038045740472031714 4 0.03765672472078394 5 0.042908437362628817 6 0.017933626132522106 7 0.017739118256898223 8 0.023768862401238627 9 0.020423326940507823 10 0.020267720640008712 11 0.018945067085766303 12 0.020423326940507823 13 0.02018991748975916 14 0.029020575043083495 15 0.02843705141621184 16 0.02155147261912635 17 0.022718519872869655 18 0.10830198514737861 19 0.02493590965498193 20 0.022718519872869655 21 0.026102956908725234 22 0.019956508039010497 23 0.022679618297744876 24 0.019022870236015857 25 0.022485110422120994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2570590.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.92298009370316 #Duplication Level Percentage of deduplicated Percentage of total 1 80.28858563826003 55.33728589697377 2 12.55362347983241 17.304662824086677 3 3.624592941952053 7.494532415578151 4 1.4393826670753695 3.968261716402282 5 0.6790356134053928 2.34005790328277 6 0.3846201262561051 1.5905499183352274 7 0.23943426552695035 1.1551766181665104 8 0.1610625666973631 0.8880729662658475 9 0.11656337122220553 0.7230505422962705 >10 0.4818769406854196 5.50877937072195 >50 0.01976679737254485 0.9393097029877123 >100 0.009532692348318373 1.2491018578175055 >500 0.0013007848652213517 0.5971733959795209 >1k 6.221145007104074E-4 0.903984871105736 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4448 0.1730342061550072 No Hit GGTATCAACGCAGAGTACTTTTTTT 2629 0.10227224100303821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.89015751247768E-5 0.0 3 0.0 0.0 0.0 3.89015751247768E-5 0.0 4 0.0 0.0 0.0 3.89015751247768E-5 0.0 5 0.0 0.0 0.0 3.89015751247768E-5 0.0 6 0.0 0.0 0.0 3.89015751247768E-5 0.0 7 0.0 0.0 0.0 3.89015751247768E-5 0.0 8 0.0 0.0 0.0 3.89015751247768E-5 0.0 9 0.0 0.0 0.0 3.89015751247768E-5 0.0 10 0.0 0.0 0.0 7.78031502495536E-5 3.89015751247768E-5 11 0.0 0.0 0.0 7.78031502495536E-5 3.89015751247768E-5 12 0.0 0.0 0.0 1.1670472537433041E-4 1.556063004991072E-4 13 0.0 0.0 0.0 1.1670472537433041E-4 1.9450787562388401E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 615 0.0 13.744165 4 GGTATCA 1075 0.0 12.410209 1 GTAGGAC 1465 0.0 11.928441 3 GTCCTAC 1470 0.0 11.668489 1 TGTAGGA 1590 0.0 11.349282 2 GTACCGT 85 5.3566866E-5 11.17012 6 GTGTAGG 515 0.0 11.102058 1 GTCCTAG 510 0.0 10.837206 1 AGGACCT 1340 0.0 10.631181 5 TCCTACA 1645 0.0 10.565672 2 GGACGTG 1085 0.0 10.413434 6 TACACCG 120 1.530485E-6 10.28862 5 AGGACGT 1090 0.0 10.28136 5 GTATCAA 2200 0.0 10.26562 1 GATATAC 475 0.0 10.231424 1 TGTACCG 130 3.8597682E-7 10.227741 5 TAGGACA 745 0.0 10.198522 4 GTATTAA 385 0.0 10.14805 1 TAGGACG 1180 0.0 10.141262 4 AATACGG 75 0.0026550894 10.130334 5 >>END_MODULE