FastQCFastQC Report
Thu 26 May 2016
SRR1512927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570590
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55160.21458108838826886No Hit
TATCAACGCAGAGTACTTTTTTTTT37070.1442081389875476No Hit
GGTATCAACGCAGAGTACTTTTTTT32250.12545757977740518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7000.012.2348514
GGTATCA12400.011.962171
GTAGGAC14400.011.9517523
TGTAGGA16650.011.4785972
TAGACCG752.0426519E-411.4191945
CTGTAGG15800.011.1332421
GTCCTAG4150.010.9976561
GGCGAGA7200.010.93931519
TGGCGAG11100.010.77189418
GTCCTAC15000.010.6493971
CTAGGAC3350.010.5020093
TAGGACG10650.010.4541924
GGACGTG10000.010.2738686
GTATTAC1951.4551915E-1110.2398051
AGGACGT10650.010.1861355
TAGGCGG750.002611869710.1503955
ATGGCGA12000.010.12432717
GTATCAA24150.010.118661
TAGGACA6500.010.1015954
TAGGACT3300.010.0927224
Produced by FastQC (version 0.11.5)