##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2570590 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.29571615854726 31.0 31.0 34.0 26.0 34.0 2 30.445024294033665 31.0 31.0 34.0 27.0 34.0 3 30.538651048980974 31.0 31.0 34.0 27.0 34.0 4 33.99792498998284 37.0 35.0 37.0 30.0 37.0 5 33.62172769675444 37.0 35.0 37.0 28.0 37.0 6 33.477470152766486 37.0 35.0 37.0 28.0 37.0 7 33.33576182899645 37.0 35.0 37.0 27.0 37.0 8 33.22240925235063 37.0 35.0 37.0 26.0 37.0 9 34.66228959110554 39.0 35.0 39.0 27.0 39.0 10 34.55478080907496 39.0 35.0 39.0 27.0 39.0 11 34.569139380453514 39.0 35.0 39.0 27.0 39.0 12 34.411704316907795 39.0 35.0 39.0 26.0 39.0 13 34.39429080483469 39.0 35.0 39.0 26.0 39.0 14 35.43518841977912 40.0 36.0 41.0 26.0 41.0 15 35.440899949038936 40.0 36.0 41.0 26.0 41.0 16 35.41305964778514 40.0 36.0 41.0 26.0 41.0 17 35.38515321385363 40.0 36.0 41.0 26.0 41.0 18 35.30656891997557 40.0 36.0 41.0 25.0 41.0 19 35.2876055691495 40.0 36.0 41.0 25.0 41.0 20 35.258628952886305 40.0 36.0 41.0 25.0 41.0 21 35.19954290649228 40.0 35.0 41.0 25.0 41.0 22 35.158149685480765 40.0 36.0 41.0 25.0 41.0 23 35.049918890215864 40.0 35.0 41.0 24.0 41.0 24 35.03128892588861 40.0 35.0 41.0 24.0 41.0 25 34.93260224306482 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.505491009152987 1101 2 0.49563719711915155 1101 3 0.49643410620326023 1101 4 0.280217124593257 1101 5 0.5358170343354232 1101 6 0.5948947725910969 1101 7 0.5929521736120833 1101 8 0.6090848607967843 1101 9 0.42463683916279393 1101 10 0.7156983546381639 1101 11 0.7404290585649989 1101 12 0.7546290528976272 1101 13 0.7934705795507497 1101 14 0.8877014253295812 1101 15 0.7732187478975021 1101 16 0.941778446339967 1101 17 0.9399637147492896 1101 18 0.41875325089510795 1101 19 0.7953016025835282 1101 20 0.5706431700290153 1101 21 0.8468540961734021 1101 22 0.7994654078549175 1101 23 0.7499664230279208 1101 24 0.8016766294327837 1101 25 0.7708300861964474 1102 1 0.6303777444923746 1102 2 0.6494081399395348 1102 3 0.5717987178156214 1102 4 0.5971235591974633 1102 5 0.7445473139329621 1102 6 0.7885730128390023 1102 7 0.8627957728790392 1102 8 0.8789837872082416 1102 9 0.9992493454149525 1102 10 1.1380237551583008 1102 11 0.9766665427881662 1102 12 1.0318204025590703 1102 13 1.183948682480377 1102 14 1.2996020203415029 1102 15 1.1845295899295252 1102 16 1.1367965075647675 1102 17 1.1393803157958828 1102 18 1.1634578255904486 1102 19 1.21939040462415 1102 20 1.096798966174525 1102 21 1.2089292550113342 1102 22 1.240884403312613 1102 23 1.2627746251465766 1102 24 1.1988917831365598 1102 25 1.1552376107899676 1103 1 0.5464259001481757 1103 2 0.485246542583603 1103 3 0.5403273775142061 1103 4 0.5530027301529756 1103 5 0.5557112536657343 1103 6 0.6545861710064358 1103 7 0.6602373540894959 1103 8 0.630907230418309 1103 9 0.802435350465835 1103 10 0.8222305741543039 1103 11 0.7302917496644028 1103 12 0.756719851086487 1103 13 0.8197257377470493 1103 14 0.9405702257956321 1103 15 0.6821088172362977 1103 16 0.8138693655013611 1103 17 0.8658067872694133 1103 18 0.9535762101152656 1103 19 0.8552889087004871 1103 20 0.9041705450065862 1103 21 0.5688537507366291 1103 22 0.7392936027793056 1103 23 0.871374718210042 1103 24 0.8896774391254354 1103 25 0.8070789696547322 1104 1 0.4869902001055131 1104 2 0.5640110215379863 1104 3 0.4749337577007573 1104 4 0.4855573519490193 1104 5 0.5036591663495571 1104 6 0.6659529198676282 1104 7 0.6256878011917024 1104 8 0.5444294752923398 1104 9 0.7911627911603887 1104 10 0.8385503902412026 1104 11 0.8774927490082121 1104 12 0.6232048647104662 1104 13 0.730228813878341 1104 14 0.8756950174273683 1104 15 0.9133463284359138 1104 16 0.8894405602940338 1104 17 1.005737176761059 1104 18 0.9637178799374695 1104 19 0.9339312855284305 1104 20 1.0069228044031462 1104 21 0.8291965724380788 1104 22 0.82864709046423 1104 23 0.7301946571061038 1104 24 0.8122795079879594 1104 25 0.9986382697574783 1105 1 0.5464134049999494 1105 2 0.6649267740644511 1105 3 0.5868860064563322 1105 4 0.5541380210351505 1105 5 0.5147842794566984 1105 6 0.6389147561021744 1105 7 0.7652264046825366 1105 8 0.846160356521537 1105 9 0.9234756684691874 1105 10 0.974964378491741 1105 11 0.8963853055324691 1105 12 0.8399863607734588 1105 13 0.8798187455352462 1105 14 1.0648726308129994 1105 15 1.0122727511429375 1105 16 1.0621511911924628 1105 17 0.8355395763912981 1105 18 1.1589698718934258 1105 19 1.1542876267592348 1105 20 1.113733197752154 1105 21 1.0425289115034744 1105 22 1.1345587217867532 1105 23 1.1124546452237425 1105 24 1.1858935791022986 1105 25 1.2555166128918032 1106 1 0.5046188928262296 1106 2 0.44118568059415253 1106 3 0.47617033486401894 1106 4 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0.7781489177830849 1107 17 0.7212959163216794 1107 18 0.9084018779800616 1107 19 1.0532219272832108 1107 20 1.0500467906087607 1107 21 0.978251033205666 1107 22 1.0088242365990396 1107 23 0.9462347032609202 1107 24 1.0010082746843239 1107 25 0.9079839693711804 1108 1 0.3988177221754441 1108 2 0.34235874131037747 1108 3 0.31369532890213847 1108 4 0.3704597110798886 1108 5 0.3995589546130418 1108 6 0.45164247307600647 1108 7 0.40324189205761485 1108 8 0.43378105902631603 1108 9 0.4298624654354697 1108 10 0.5670036718507703 1108 11 0.5481673948628014 1108 12 0.6193317105105152 1108 13 0.5644247207142712 1108 14 0.5949495578120505 1108 15 0.6606352737061556 1108 16 0.5157945555567736 1108 17 0.6167364444162402 1108 18 0.5169109339992275 1108 19 0.5340618206304271 1108 20 0.5975573704658572 1108 21 0.6299589464609738 1108 22 0.6520723192836897 1108 23 0.540600081973345 1108 24 0.6245663542782793 1108 25 0.5865365014051918 1109 1 0.17731397725648534 1109 2 0.16525534591118785 1109 3 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-2.2057494091343557 2216 17 -2.47974069102888 2216 18 -2.1859746848278903 2216 19 -2.3722536048059766 2216 20 -2.791835466299446 2216 21 -2.5508084538500313 2216 22 -2.912624274990378 2216 23 -2.0056460726164786 2216 24 -2.159997541946801 2216 25 -2.1041141614207177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75256.0 3 3.0 4 1209.0 5 10412.0 6 12147.0 7 9432.0 8 7743.0 9 6741.0 10 5802.0 11 5290.0 12 4780.0 13 4447.0 14 4394.0 15 4380.0 16 4586.0 17 4822.0 18 5187.0 19 6153.0 20 7768.0 21 9953.0 22 12639.0 23 15425.0 24 18157.0 25 21093.0 26 23959.0 27 26235.0 28 29693.0 29 35348.0 30 44575.0 31 56904.0 32 72152.0 33 92104.0 34 120820.0 35 157460.0 36 216985.0 37 338548.0 38 1097988.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4517434612902 18.582502863398563 13.252960921519911 27.712792753791327 2 14.08013581339041 19.53854101749562 39.51775976372703 26.86356340538694 3 17.686134311578275 23.328185358225152 29.41110795576113 29.574572374435444 4 11.751537084598024 16.17978052766752 36.29796093081173 35.770721456922715 5 13.19841952999354 37.627729937246045 35.47223918994099 13.701611342819422 6 33.69849542421625 36.54460294218983 16.653696073900846 13.103205559693082 7 28.946874785954908 31.141420761284454 20.894381588697172 19.017322864063463 8 25.75926336144288 37.194726705678214 19.120655630740146 17.925354302138757 9 25.868466480224043 15.282184220103497 19.121695654797005 39.72765364487545 10 14.754391417428266 28.96816587488345 33.72178615076518 22.555656556923097 11 35.67006074007736 22.21360292959123 23.178254795540568 18.93808153479084 12 23.162723812406693 24.40333875838123 30.832818128868166 21.601119300343907 13 29.01863263179067 21.116736299609826 25.516897676338505 24.347733392261 14 21.212098812022557 21.02508038835405 26.73865468898023 31.024166110643165 15 23.341572188281635 29.834212658942917 22.87062300294008 23.953592149835373 16 23.160183434977935 27.957219775678908 25.948321949708685 22.93427483963447 17 22.300189318395404 28.166413398994845 26.807368046245834 22.72602923636392 18 22.339647480435897 27.56924269137343 27.996626368787066 22.09448345940361 19 23.736516481725207 26.758396282678575 26.872267261840616 22.6328199737556 20 23.71812728079117 26.803585462071755 27.33447972673301 22.143807530404064 21 23.327594027491095 26.34306370007057 26.805605587537123 23.523736684901216 22 23.382275766745988 26.922267465088495 26.938839658581692 22.756617109583825 23 22.90746482325069 26.802368327893593 27.106150728042977 23.184016120812732 24 23.249176259146733 26.423311379877774 27.432885057515975 22.894627303459515 25 23.074663690301357 26.664625537790126 27.205848884621624 23.054861887286894 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 113.0 2 267.0 3 421.0 4 421.0 5 421.0 6 1136.0 7 1851.0 8 1851.0 9 1851.0 10 3019.0 11 4187.0 12 4187.0 13 4187.0 14 7045.0 15 9903.0 16 9903.0 17 9903.0 18 17673.5 19 25444.0 20 25444.0 21 25444.0 22 39602.0 23 53760.0 24 53760.0 25 53760.0 26 76463.5 27 99167.0 28 99167.0 29 99167.0 30 128970.0 31 158773.0 32 158773.0 33 158773.0 34 186023.5 35 213274.0 36 213274.0 37 213274.0 38 239037.0 39 264800.0 40 264800.0 41 264800.0 42 286689.0 43 308578.0 44 308578.0 45 308578.0 46 322738.0 47 336898.0 48 336898.0 49 336898.0 50 334878.0 51 332858.0 52 332858.0 53 332858.0 54 306377.5 55 279897.0 56 279897.0 57 279897.0 58 245577.0 59 211257.0 60 211257.0 61 211257.0 62 174554.5 63 137852.0 64 137852.0 65 137852.0 66 107129.5 67 76407.0 68 76407.0 69 76407.0 70 55609.0 71 34811.0 72 34811.0 73 34811.0 74 24190.5 75 13570.0 76 13570.0 77 13570.0 78 9222.0 79 4874.0 80 4874.0 81 4874.0 82 3155.5 83 1437.0 84 1437.0 85 1437.0 86 912.0 87 387.0 88 387.0 89 387.0 90 227.0 91 67.0 92 67.0 93 67.0 94 35.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07640269354506164 2 1.556063004991072E-4 3 0.0 4 0.030732244348573676 5 0.07729742977293151 6 0.07620818566943775 7 0.04037983497951832 8 0.07581916991818999 9 0.05582376030405471 10 0.11545987497033755 11 0.0050183031910962074 12 0.016066350526532817 13 0.008052626050828798 14 0.009919901656818084 15 0.009686492206069423 16 0.020384425365383044 17 0.0328718309804364 18 0.002567503958235269 19 0.005329515792094422 20 0.008169330776203129 21 4.668189014973216E-4 22 7.391299273707592E-4 23 0.0 24 0.0 25 0.004551484289598885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2570590.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.65564707740283 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3678632804122 53.69684147387128 2 13.004627322472187 17.596729530046574 3 3.8343556806391317 7.782474440956675 4 1.548560305998552 4.190753981628519 5 0.7372665063039026 2.4940121276243317 6 0.41400191710692785 1.6805740555892696 7 0.2565811802049603 1.2151416042255205 8 0.17241346463590276 0.933179561183913 9 0.1161426245924978 0.7071933978065986 >10 0.5158196725588332 5.780284626033499 >50 0.020200820752839403 0.9500836695718662 >100 0.010154470028143764 1.2991895921295848 >500 0.0013226671074956097 0.5876111012873905 >1k 6.325799209253131E-4 0.8713203080510857 >5k 5.750726553866483E-5 0.21461052999373914 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5516 0.21458108838826886 No Hit TATCAACGCAGAGTACTTTTTTTTT 3707 0.1442081389875476 No Hit GGTATCAACGCAGAGTACTTTTTTT 3225 0.12545757977740518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.78031502495536E-5 2 0.0 0.0 0.0 0.0 7.78031502495536E-5 3 0.0 0.0 0.0 0.0 7.78031502495536E-5 4 0.0 0.0 0.0 0.0 7.78031502495536E-5 5 0.0 0.0 0.0 0.0 7.78031502495536E-5 6 0.0 0.0 0.0 0.0 7.78031502495536E-5 7 0.0 0.0 0.0 0.0 7.78031502495536E-5 8 0.0 0.0 0.0 0.0 7.78031502495536E-5 9 0.0 0.0 0.0 3.89015751247768E-5 7.78031502495536E-5 10 0.0 0.0 0.0 7.78031502495536E-5 7.78031502495536E-5 11 0.0 0.0 0.0 7.78031502495536E-5 7.78031502495536E-5 12 0.0 3.89015751247768E-5 0.0 7.78031502495536E-5 1.1670472537433041E-4 13 0.0 3.89015751247768E-5 0.0 7.78031502495536E-5 1.1670472537433041E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 700 0.0 12.234851 4 GGTATCA 1240 0.0 11.96217 1 GTAGGAC 1440 0.0 11.951752 3 TGTAGGA 1665 0.0 11.478597 2 TAGACCG 75 2.0426519E-4 11.419194 5 CTGTAGG 1580 0.0 11.133242 1 GTCCTAG 415 0.0 10.997656 1 GGCGAGA 720 0.0 10.939315 19 TGGCGAG 1110 0.0 10.771894 18 GTCCTAC 1500 0.0 10.649397 1 CTAGGAC 335 0.0 10.502009 3 TAGGACG 1065 0.0 10.454192 4 GGACGTG 1000 0.0 10.273868 6 GTATTAC 195 1.4551915E-11 10.239805 1 AGGACGT 1065 0.0 10.186135 5 TAGGCGG 75 0.0026118697 10.150395 5 ATGGCGA 1200 0.0 10.124327 17 GTATCAA 2415 0.0 10.11866 1 TAGGACA 650 0.0 10.101595 4 TAGGACT 330 0.0 10.092722 4 >>END_MODULE