FastQCFastQC Report
Thu 26 May 2016
SRR1512926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338462
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT34010.14543747129523593No Hit
GTCCTAAAGTGTGTATTTCTCATTT31680.13547365747230444No Hit
CTGTAGGACGTGGAATATGGCAAGA31070.1328651053555713No Hit
CTTTAGGACGTGAAATATGGCGAGG27750.11866773973663032No Hit
GTATCAACGCAGAGTACTTTTTTTT27370.11704274005735393No Hit
GTCCTACAGTGTGCATTTCTCATTT25760.11015787299515664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.012.6723551
TAGGACC7150.011.823474
TAGTACC907.4769378E-611.6094374
GACGTGA9450.010.9521087
TACGACC700.001493684810.8555774
CGTGCGC803.7869535E-410.68298810
GGACGTG17000.010.6681116
AGGACGT17200.010.6576635
TTAGGAC11400.010.5818083
CTTATAC2350.010.5091243
GTCCTAA9850.010.4482721
GGCGAGG8300.010.29641519
AGGACCT13550.010.234035
TGTAGGA19900.010.2146082
GTCCTAG3000.010.1644931
GTCCTAC19600.010.1612511
TACACCG750.002653005310.1312235
GTATAAT3400.010.0897541
GTCCTAT2550.010.0897531
GGACCTG13000.010.0794956
Produced by FastQC (version 0.11.5)